Researchers Database

OGAWA, Atsushi

    Proteo-Science Center Associate Professor
Last Updated :2020/10/15

Researcher Information

Degree

  • Doctor of Engineering (Ph. D.)(Kyoto University)

URL

J-Global ID

Research Interests

  • Bio-related Chemistry   biomolecular engineering   bioengineering   life science   biotechnology   protein engineering   RNA engineering   

Research Areas

  • Nanotechnology/Materials / Biochemistry

Academic & Professional Experience

  • 2013/04 - Today  Ehime UniversityAssociate Professor (PI)
  • 2010/04 - 2013/03  Ehime UniversityLecturer (PI)
  • 2008/12 - 2013/03  Ehime UniversitySenior Research Fellow (PI)
  • 2008/12 - 2010/03  RIKENVisiting Researcher
  • 2006/04 - 2008/12  RIKENSpecial Postdoctoral Researcher
  • 2003/04 - 2006/03  Japan Society for the Promotion of Science (JSPS)JSPS Fellows DC1

Education

  • 2003/04 - 2006/03  Kyoto University  Graduate School of Engineering  Department of Synthetic Chemistry and Biological Chemistry
  • 2001/04 - 2003/03  Kyoto University  Graduate School of Engineering  Department of Molecular Engineering
  • 1997/04 - 2001/03  Kyoto University  Faculty of Engineering  Undergraduate School of Industrial Chemistry

Association Memberships

  • Japanese Society for Cell Synthesis Research   Division of Biofunctional Chemistry, The Chemical Society of Japan   無細胞生命科学研究会   JAPANESE SOCIETY FOR CHEMICAL BIOLOGY   THE CHEMICAL SOCIETY OF JAPAN   日本癌学会   

Published Papers

  • Atsushi Ogawa, Yu Itoh
    ACS Synthetic Biology 2161-5063 2020/10 [Peer-reviewed]
     Scientific journal
  • Mari Tezuka-Kagajo*, Masashi Maekawa*, Atsushi Ogawa*, Yoshiko Hatta, Eiichi Ishii, Mariko Eguchi, Shigeki Higashiyama, *These authors equally contributed to this work.
    Nucleic acid therapeutics 2020/09 [Peer-reviewed]
     Scientific journal 
    C promoter binding factor 1 (CBF1) (alias RBPJ) is a critical transcription factor involved in Notch signaling. The activation of Notch signaling through CBF1 maintains the angiostatic state of endothelial cells suppressing angiogenesis, that is, the formation of new blood vessels. Vascular endothelial growth factor (VEGF) induces angiogenesis by promoting the proteasomal degradation of CBF1, in addition to endothelial cell proliferation. To date, angiogenic inhibitors targeting VEGF have been successfully used in clinics for cancer and age-related macular degeneration. Most antiangiogenic drugs, however, only target VEGF or VEGF receptors. In this study, to expand the repertoire of antiangiogenic therapeutics, we developed 15 single-stranded deoxyribonucleic acid (ssDNA) aptamers capable of binding to CBF1 with high affinity (Kd; 10-300 nM). To this end, systematic evolution of ligands by the exponential enrichment (SELEX) method was applied. One of the CBF1-binding ssDNA aptamers, Apt-3, inhibited angiogenesis through the activation of Notch signaling in vitro. We found that Apt-3 directly interacted with the LAG1 domain of CBF1. We suggest that the Apt-3 ssDNA aptamer may contribute to the development of a novel angiogenic inhibitor, which does not target VEGF.
  • Atsushi Ogawa
    Analytical Sciences 0910-6340 2020/09 [Peer-reviewed]
     Scientific journal
  • Hajime Takahashi, Atsushi Ogawa
    ACS synthetic biology 9 (7) 1608 - 1614 2020/06 [Peer-reviewed]
     Scientific journal 
    We sought to prepare millimeter-sized supergiant unilamellar vesicles (SGUVs) by spontaneous emulsion transfer for efficient, eukaryotic cell-free translation in the interior. Although the conventional protocols require that a considerably high concentration of sucrose be encapsulated into the SGUVs for their efficient formation, such high amounts of sucrose severely inhibited cell-free translation based on wheat germ extract (WGE). We thus optimized the preparation conditions to permit SGUV formation at a much lower concentration of sucrose that has almost no effect on WGE translation. Under the optimized conditions, we successfully prepared WGE translation system-encapsulating SGUVs that allow for protein synthesis with a high efficiency comparable to that outside a liposome. The optimization also resulted in a high rate of successful SGUV formation (>90%) and a decent stability of the formed SGUVs (>60 min). These SGUVs are expected to serve as research tools in cell-free synthetic biology and as foundations for artificial cell-based biosensors.
  • Atsushi Ogawa, Masashi Takamatsu
    Bioorganic & medicinal chemistry letters 29 (22) 126729  0960-894X 2019/11 [Peer-reviewed]
     Scientific journal 
    Wheat germ extract (WGE) is one of the most widely used eukaryotic cell-free translation systems for easy synthesis of a broad range of proteins merely by adding template mRNAs. Its productivity has thus far been improved by removing translational inhibitors from the extract and stabilizing the template with terminal protectors. Nonetheless, there remains room for increasing the yield by designing a terminally protected template with higher susceptibility to translation. Given the fact that a 5′ terminal protector is a strong inhibitor of the canonical translation, we herein focused on Cripavirus internal ribosome entry sites (IRESes), which allow for a unique translation initiation from a non-AUG start codon without the help of any initiation factors. We mutated their start codons to enhance the IRES-mediated translation efficiency in WGE. One of the mutants showed considerably higher efficiency, 3–4-fold higher than that of its wild type, and also 3–4-fold higher than the canonical translation efficiency by an IRES-free mRNA having one of the most effective canonical-translation enhancers. Because this mutated IRES is compatible with different types of genes and terminal protectors, we expect it will be widely used to synthesize proteins in WGE.
  • Atsushi Ogawa, Akane Kutsuna, Masashi Takamatsu, Tatsuya Okuzono
    Bioorganic & medicinal chemistry letters 29 (16) 2141 - 2144 0960-894X 2019/08 [Peer-reviewed]
     Scientific journal 
    Wheat cell-free expression systems based on wheat germ extract (WGE) enable us to briefly synthesize various types of proteins in vitro merely by exogenously adding their mRNA templates. Moreover, it is possible to produce larger amounts of protein by thoroughly removing the endosperm, which contains many translation inhibitors, including ribonucleases (RNases). However, because small amounts of RNases are also present even in an endosperm-free, high-quality WGE (hqWGE), the in-vitro transcribed mRNA is rapidly degraded. In particular, 3′ exonucleases have been considered as the major RNases that degrade mRNA. We thus herein performed in vitro selection to find an effective, short 3′ protector sequence from a random RNA pool. The selected sequences stabilized in vitro transcripts in the hqWGE more effectively than the previously reported, longer 3′ protectors did. In addition, when one of these 3′ protectors was minimized and then fused to mRNA, the translation efficiency increased 5–6-fold in the hqWGE, mainly due to the mRNA stabilization.
  • Yuki Yano, Masamichi Nisougi, Yuki Yano-Ozawa, Tsuyoshi Ohguni, Atsushi Ogawa, Mizuo Maeda, Tsuyoshi Asahi, Tamotsu Zako
    Analytical Sciences 35 (6) 685 - 690 1348-2246 2019/06 [Peer-reviewed]
     Scientific journal 
    Gold nanoparticles (AuNPs) have been commonly used in molecular sensing, in the form of observation of the color change from red to blue of the AuNP solution, caused by target-molecule-induced AuNP aggregation. In this work, the changes in absorbance and scattering spectra caused by AuNP aggregation were studied using thrombin-induced AuNP aggregation as a model. We demonstrated for the first time that scattering spectra is more sensitive to the changes owing to AuNP aggregation than absorbance spectra. Moreover, a digital color analysis of darkfield images using dark field microscopy (DFM) facilitated a simple method for detection of AuNPs aggregation without the use of spectroscopic analysis. Furthermore, we demonstrated that DFM is useful for detecting AuNPs aggregation in a colored solution, in which the color change by AuNPs aggregation is not visible.
  • Sawa Naruhiko, Tatsuke Tsuneyuki, Ogawa Atsushi, Hirokawa Yasutaka, Osanai Takashi, Hanai Taizo
    Journal of bioscience and bioengineering Society for Biotechnology 127 (2) 256 - 264 1389-1723 2019/02 [Peer-reviewed]
     Scientific journal 
    Many cyanophages, which infect cyanobacteria, most of possess putative sigma factors that have high amino acid sequence homology with the σ70-type sigma factor present in cyanobacteria, allowing them to obtain energy and metabolites for their own propagation. In this study, we aimed to modify the carbon metabolism of Synechococcus elongatus PCC 7942 by expressing putative sigma factors from Synechococcus phages to improve bioproduction. Four cyanophage-derived putative sigma factors—putative RpsD4 from Synechococcus phage S-CBS1, putative RpoD and putative RpoS from S-CBS2, and putative RpsD4 from S-CBS3—were selected for this purpose. These were introduced into S. elongatus PCC 7942, and their expression was controlled with a theophylline-dependent riboswitch. The expression of the putative RpoD from S-CBS2 and putative RpsD4 from S-CBS3 resulted in a significant decrease in the growth rate of S. elongatus PCC 7942. In addition, metabolome analysis showed a 3.2-fold increase in acetyl-CoA concentration with the expression of the putative RpoD from S-CBS2 and a 1.9-fold increase with the putative RpsD4 from S-CBS3. The results of RT-qPCR showed that several sugar metabolism genes were repressed by the putative RpoD and activated by the putative RpsD4. In particular, the engineered strain overexpressing the putative RpsD4 and expressing phosphate acetyltransferase succeeded in improving the productivity of the model target product acetate to 217% of its previous value. To the best of our knowledge, this study is the first to modify the metabolism of S. elongatus PCC 7942 by expressing their putative sigma factors from cyanophages.
  • Atsushi Ogawa, Yuta Murashige, Hajime Takahashi
    Bioorganic and Medicinal Chemistry Letters 28 (14) 2353 - 2357 1464-3405 2018 [Peer-reviewed]
     Scientific journal 
    We have found that OFF-riboswitches that ligand-dependently downregulate the canonical translation in a higher eukaryotic expression system (wheat germ extract) can be easily created by inserting a single aptamer into the 5′ untranslated region (UTR) of mRNA, even if its ligand is as small as theophylline. The key is the position of the inserted aptamer: the 5′ end (+0 position) is much better than other positions for inhibiting canonical translation with the aptamer-ligand complex. The data showed that ribosome loading is suppressed by a rigid structure in the 5′ end, and this suppression is dependent on the structure's stability but not on its size. Although this preference of aptamer insertion point contradicts the results in a lower eukaryote, it accords with the fact that the 5′-end structural hindrance is more effective for blocking the ribosome in higher eukaryotes. Therefore, the present type of OFF-riboswitch would function in various higher eukaryotic expression systems.
  • Atsushi Ogawa, Hiroki Masuoka, Tsubasa Ota
    ACS SYNTHETIC BIOLOGY 6 (9) 1656 - 1662 2161-5063 2017/09 [Peer-reviewed]
     Scientific journal 
    We constructed novel artificial riboswitches that function in a eukaryotic translation system (wheat germ extract), by rationally implanting an in vitro-selected aptamer into the intergenic internal ribosome entry site (IRES) of Plautia stali intestine virus. These eukaryotic OFF-riboswitches (OFF-eRSs) ligand-dose-dependently downregulate IRES-mediated translation without hybridization switches, which typical riboswitches utilize for gene regulation. The hybridization-switch-free mechanism not only allows for easy design but also requires less energy for regulation, resulting in a higher switching efficiency than hybridization-switch-based OFF-eRSs provide. In addition, even a small ligand such as theophylline can induce satisfactory repression, in contrast to other types of OFF-eRSs that modulate the 5' cap-dependent canonical translation. Because our proposed hybridization-switch-free OFF-eRSs are based on a versatile IRES that functions well in many types of eukaryotic translation systems, they would be widely usable elements for synthetic gene circuits in both cell-free and cell-based synthetic biology.
  • Atsushi Ogawa, Yuta Murashige, Junichiro Tabuchi, Taiki Omatsu
    MOLECULAR BIOSYSTEMS 13 (2) 314 - 319 1742-206X 2017/02 [Peer-reviewed]
     Scientific journal 
    We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 30 cap-independent translation element (30 CITE) with no major hybridization switches in a plant expression system (wheat germ extract).
  • Atsushi Ogawa, Junichiro Tabuchi, Yasunori Doi, Masashi Takamatsu
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 26 (15) 3658 - 3661 0960-894X 2016/08 [Peer-reviewed]
     Scientific journal 
    We have developed a novel type of biofunction-assisted, signal-turn-on sensor for simply and homogenously detecting DNA. This sensor system is composed of two types of in vitro-transcribed label-free RNAs (a 3' premature amber suppressor tRNA probe and an amber-mutated mRNA encoding a reporter protein), RNase H, and a wheat germ extract (WGE). A target DNA induces the 3' end maturation of the tRNA probe, which is enhanced by RNase H and leads to the expression of a full-length reporter protein through amber suppression in WGE, while there is almost no expression without the target due to the inactivity of the premature probe. Therefore, the target can be readily detected with the activity of the translated reporter. The catalytic reuse of the target with the help of RNase H in addition to various bioprocesses in WGE enables this sensor system to exhibit relatively high selectivity and sensitivity. (C) 2016 Elsevier Ltd. All rights reserved.
  • Atsushi Ogawa, Yuki Namba, Mai Gakumasawa
    ORGANIC & BIOMOLECULAR CHEMISTRY 14 (9) 2671 - 2678 1477-0520 2016 [Peer-reviewed]
     Scientific journal 
    Amber suppression is a useful method of genetically incorporating a non-natural amino acid (NAA) into a protein during translation by utilizing an NAA-charged amber suppressor tRNA (sup-tRNA). A wheat germ extract (WGE) is suitable for this method by virtue of its high productivity and versatility in addition to its advantages as a cell-free translation system. However, in spite of this high potential, a genetic NAA incorporation system in WGE has not been sufficiently optimized in terms of sup-tRNAs, in contrast to that in E. coli and its cell extracts. We herein rationally optimized amber sup-tRNAs to efficiently incorporate a model NAA, p-acetyl-phenylalanine (AcPhe), into a protein in WGE, via flexizyme-based aminoacylation. The optimized sup-tRNA (named tLys-opt) that was pre-charged with AcPhe exclusively yielded up to 220 mu g mL(-1) of AcPhe-incorporated protein (yellow fluorescent protein, YPet) under the optimal conditions. This high productivity is comparable to the best reported yield of a similar NAA-incorporated protein synthesized with an engineered aminoacyl-tRNA synthetase/sup-tRNA pair in WGE, despite the fact that tLys-opt that has released AcPhe was not reused at all in this study. The results clearly show both the necessity of optimizing sup-tRNAs for efficient NAA incorporation and the validity of our strategy for their optimization. Because the optimization strategy described here is expected to be applicable not only to amber sup-tRNAs for other NAAs but also to ones used in other acylation methods, it would facilitate the synthesis of large amounts of various types of NAA-incorporated proteins in WGE.
  • Atsushi Ogawa
    RIBOSWITCHES AS TARGETS AND TOOLS 550 109 - 128 0076-6879 2015 [Invited]
     In book 
    A number of natural and artificial bacterial riboswitches have been reported thus far. However, they generally function only in bacteria, not in eukaryotes. This is because of the differences of expression mechanisms (transcription, translation, and so on) between these two main types of organisms. For example, the mechanism of translation initiation is quite different between bacteria and eukaryotes, especially in ribosome loading on mRNA. While the bacterial ribosome binds to a well-conserved, internal sequence some bases before the start codon to initiate translation, the eukaryotic one is loaded on the 50 terminus with the help of certain eukaryotic initiation factors. This means not only that bacterial riboswitches regulating translation initiation are not available in eukaryotic translation systems, but also that it is physically difficult to construct eukaryotic ON riboswitches that regulate the eukaryotic canonical translation initiation, because an aptamer cannot be inserted upstream of the ribosome loading site. However, the mechanism of noncanonical translation initiation via "ribosomal shunt" enables us to design translation initiation-modulating (specifically, ribosomal shunt-modulating) eukaryotic ON riboswitches. This chapter describes a facile method for engineering these ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation system. Because these riboswitches do not require hybridization switching thanks to a unique shunting mechanism, they have the major advantages of a low energy requirement for upregulation and relatively straightforward design over common hybridization switch-based ON riboswitches.
  • Atsushi Ogawa, Yasunori Doi
    ORGANIC & BIOMOLECULAR CHEMISTRY 13 (4) 1008 - 1012 1477-0520 2015 [Peer-reviewed]
     Scientific journal 
    We investigated the end processing and degradation of premature tRNAs in wheat germ extract (WGE), which led to the discovery of end protectors useful for stabilizing an in vitro transcript against various ribonucleases and thereby enhancing its apparent activity in WGE.
  • Atsushi Ogawa, Junichiro Tabuchi
    ORGANIC & BIOMOLECULAR CHEMISTRY 13 (24) 6681 - 6685 1477-0520 2015 [Peer-reviewed]
     Scientific journal 
    We have developed a novel type of biofunction-assisted aptasensor that harnesses ligand-dependent 3' processing of a premature amber suppressor tRNA and the subsequent amber suppression of a reporter gene in a wheat germ extract.
  • Ayumi Kimura, Naoki Kanayama, Atsushi Ogawa, Hideaki Shibata, Hideo Nakashita, Tohru Takarada, Mizuo Maeda
    ANALYTICAL CHEMISTRY 86 (22) 11425 - 11433 0003-2700 2014/11 [Peer-reviewed]
     Scientific journal 
    Diblock copolymers composed of allele-specific oligodeoxyribonucleotide (ODN) and poly(ethylene glycol) (PEG) are used as an affinity probe of free-solution capillary electrophoresis to quantitatively detect single-base substitutions in genetic samples. During electrophoresis, the probe binds strongly to a wild-type single-stranded DNA analyte (WT) through hybridization, while it binds weakly to its single-base-mutated DNA analyte (MT) due to a mismatch. Complex formation with the probe augments the hydrodynamic friction of either analyte, thereby retarding its migration. The difference in affinity strength leads to separation of the WT, MT, and contaminants, including the PCR primers used for sample preparation. The optimal sequence of the probe's ODN segment is rationally determined in such a way that the binding constant between the ODN segment and MT at the capillary temperature is on the order of 106 M. The validity of this guideline is verified using various chemically synthesized DNA analytes, as well as those derived from a bacterial genome. The peak area ratio of MT agrees well with its feed ratio, suggesting the prospective use of the present method in SNP allele frequency estimation.
  • Atsushi Ogawa, Junichiro Tabuchi, Yasunori Doi
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 24 (16) 3724 - 3727 0960-894X 2014/08 [Peer-reviewed]
     Scientific journal 
    High-quality wheat germ extract (hqWGE) is very useful for the high-yield production of various types of protein. The most important key to high productivity is the design of mRNA templates. Although the design has been refined for straightforward and efficient translation in hqWGE, there is still room for improvement in untranslated regions (UTRs), especially the 3' UTR length, because a long, cumbersome 3' UTR is commonly used for translation enhancement. Here we examined some short viral 3' cap-independent translation enhancers (3' CITEs) to identify effective ones for efficient translation in hqWGE. We then combined the most effective 3' CITE and a 5' enhancer to further increase the translation efficiency. mRNA with the optimal short 3' and 5' UTRs, both of whose length was less than 150 nt, exhibited a productivity of 1.4 mg/mL in prolonged large-scale protein synthesis in hqWGE, which was comparable to that of control mRNA with a commonly-used long 30 UTR (similar to 1200 nt). (C) 2014 Elsevier Ltd. All rights reserved.
  • Atsushi Ogawa
    Methods in Molecular Biology 1111 165 - 181 1064-3745 2014 [Invited]
     Scientific journal 
    Riboswitches are composed of two regions: one for binding to the ligand (the aptamer domain) and the other for regulating the expression of the gene (the expression platform). In most riboswitches (both natural and artificial), a part of the aptamer domain required for ligand binding is directly involved in the regulation of expression, so that it is difficult to design other ligand-responsive riboswitches based on these riboswitches even by using artificial aptamers obtained through in vitro selection. This chapter describes a method for rationally constructing a foundational ON-riboswitch, which is easily available for the design of other ligand-dependent riboswitches, by introducing a new region (a modulator sequence: MS) in addition to the two basic regions. A facile method for preparing arbitrary molecule-dependent riboswitches based on the foundational riboswitch is also presented. © Springer Science+Business Media New York 2014.
  • Yoichi Nakahira, Atsushi Ogawa, Hiroyuki Asano, Tokitaka Oyama, Yuzuru Tozawa
    Plant and Cell Physiology 54 (10) 1724 - 1735 0032-0781 2013/10 [Peer-reviewed]
     Scientific journal 
    The cyanobacterium Synechococcus elongatus PCC 7942 is a major model species for studies of photosynthesis. It is are also a potential cell factory for the production of renewable biofuels and valuable chemicals. We employed engineered riboswitches to control translational initiation of target genes in this cyanobacterium. A firefly luciferase reporter assay revealed that three theophylline riboswitches performed as expected in the cyanobacterium. Riboswitch-E* exhibited very low leaky expression of luciferase and superior and dose-dependent on/off regulation of protein expression by theophylline. The maximum magnitude of the induction vs. basal level was ∼190-fold. Furthermore, the induction level was responsive to a wide range of theophylline concentrations in the medium, from 0 to 2 mM, facilitating the fine-tuning of luciferase expression. We adapted this riboswitch to another gene regulation system, in which expression of the circadian clock kaiC gene product is controlled by the theophylline concentration in the culture medium. The results demonstrated that the adequately adjusted expression level of KaiC restored complete circadian rhythm in the kaiC-deficient arrhythmic mutant. This theophylline-dependent riboswitch system has potential for various applications as a useful genetic tool in cyanobacteria. © 2013 The Author.
  • Atsushi Ogawa
    CHEMBIOCHEM 14 (13) 1539 - 1543 1439-4227 2013/09 [Peer-reviewed]
     Scientific journal 
    A new response: A new type of hybridization switch-free ON-eRS (eukaryotic upregulating riboswitch) activates ribosomal shunting in response to a ligand. Whereas the ribosome that has finished sORF translation scans the 5′-side strand of the aptamer and stops at the rigid stem in the absence of the ligand, it shunts over the stem bearing the aptamer-ligand complex and then reinitiates translation of dORF in the presence of the ligand. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
  • Hideaki Shibata, Atsushi Ogawa, Naoki Kanayama, Tohru Takarada, Mizuo Maeda
    ANALYTICAL CHEMISTRY 85 (11) 5347 - 5352 0003-2700 2013/06 [Peer-reviewed]
     Scientific journal 
    A sample preparation method was developed for single-nucleotide polymorphism (SNP) genotyping based on hybridization between a single-stranded DNA (ssDNA) analyte and an allele-specific oligonucleotide (ASO) probe. When the SNP site is located in the stable secondary structure, the folding of this analyte imposes kinetic penalties on the hybridization with the ASO probe. To address this issue, the sequence of the ssDNA analyte was converted from the original one so that the analyte exhibited a clear dumbbell-shaped structure composed of two stemloop moieties and an unfolded probe-binding site. The as-prepared analyte was structurally favorable for hybridization with the ASO probe, irrespective of the original sequence and secondary structure of the analyte. The sequence conversion was easily achieved by polymerase chain reaction using forward and reverse primers having an additional sequence at the 5'-terminus. These ssDNA analytes were subjected to affinity capillary electrophoresis using a diblock copolymer probe composed of an ASO segment and a poly(ethylene glycol) segment. The 70-base dumbbell-shaped analytes with a single-base difference were clearly separated within 12 min, although the original ones exhibited almost no separation due to the undesired folding of the probe-binding site. This sample preparation method should open up a wide range of applications for the ASO probes in genetic analysis.
  • Atsushi Ogawa, Yukiko Susaki
    ORGANIC & BIOMOLECULAR CHEMISTRY 11 (20) 3272 - 3276 1477-0520 2013 [Peer-reviewed]
     Scientific journal 
    We report a facile design method for constructing multiple-input and visible-output logic gates by combining signal-converting DNA machines and the gold nanoparticle aggregation-based foundational AND gate.
  • Atsushi Ogawa
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 22 (4) 1639 - 1642 0960-894X 2012/02 [Peer-reviewed]
     Scientific journal 
    A strategy for rationally constructing a novel type of eukaryotic OFF-riboswitch, which ligand-dependently turns off translation mediated by an internal ribosome entry site (IRES), has been established. The theophylline-dependent IRES-based OFF-riboswitch obtained through the proposed strategy functioned well in wheat germ extract, independently from the downstream gene, indicating that it can regulate any gene. Despite the fact that it has one theophylline aptamer, its switching efficiency was as high as that of a previously reported theophylline-dependent OFF-riboswitch that was constructed by inserting three continuous theophylline aptamers into a 5' untranslated region in mRNA to downregulate the normal 5'-terminus-mediated translation. In addition, because the riboswitch part that was optimized in the theophylline-dependent IRES-based OFF-riboswitch, except for the aptamer domain, can be used as-is for other aptamer-ligand pairs, an arbitrary ligand-dependent IRES-based OFF-riboswitch is easy to construct with the corresponding well-minimized aptamer. (C) 2011 Elsevier Ltd. All rights reserved.
  • Atsushi Ogawa, Masayoshi Hayami, Shinsuke Sando, Yasuhiro Aoyama
    Journal of Nucleic Acids 2012 (538129) 1 - 7 2090-0201 2012 [Peer-reviewed][Invited]
     Scientific journal 
    Here is presented a concept for in vitro selection of suppressor tRNAs. It uses a pool of dsDNA templates in compartmentalized water-in-oil micelles. The template contains a transcription/translation trigger, an amber stop codon, and another transcription trigger for the anticodon- or anticodon loop-randomized gene for tRN A Ser. Upon transcription are generated two types of RNAs, a tRNA and a translatable mRNA (mRNA-tRNA). When the tRNA suppresses the stop codon (UAG) of the mRNA, the full-length protein obtained upon translation remains attached to the mRNA (read-through ribosome display) that contains the sequence of the tRNA. In this way, the active suppressor tRNAs can be selected (amplified) and their sequences read out. The enriched anticodon (CUA) was complementary to the UAG stop codon and the enriched anticodon-loop was the same as that in the natural tRN A Ser. © 2012 Atsushi Ogawa et al.
  • Atsushi Ogawa
    RNA-A PUBLICATION OF THE RNA SOCIETY 17 (3) 478 - 488 1355-8382 2011/03 [Peer-reviewed]
     Scientific journal 
    Riboswitches are RNA elements in mRNA that control gene expression in cis in response to their specific ligands. Because artificial riboswitches make it possible to regulate any gene with an arbitrary molecule, they are expected to function as biosensors, in which the output is easily detectable protein expression. I report herein a fully rational design strategy for artificially constructing novel riboswitches that work in a eukaryotic cell-free translation system (wheat germ extract). In these riboswitches, translation mediated by an internal ribosome entry site (IRES) is promoted only in the presence of a specific ligand (ON), while it is inhibited in the absence of the ligand (OFF). The first rationally designed riboswitch, which is regulated by theophylline, showed a high switching efficiency and dependency on theophylline. In addition, based on the design of the theophylline-dependent riboswitch, other three kinds of riboswitches controlled by FMN, tetracycline, and sulforhodamine B, were constructed only by calculating the Delta G value of one stem-loop structure. The rational design strategy described herein is therefore useful for easily producing various ligand-dependent riboswitches, which are available as biosensors for detecting their ligands.
  • Atsushi Ogawa
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 21 (1) 155 - 159 0960-894X 2011/01 [Peer-reviewed]
     Scientific journal 
    A single-step sensing system was developed to visually detect ligands of a cleavase-like RNA aptazyme at room temperature using aptazyme-tethered gold nanoparticles, the electrosteric stability of which was adjusted by increasing their diameter. In this system, the ligand induces self-cleavage of the aptazyme on gold nanoparticles to decrease the electrosteric stability of the gold nanoparticles, which causes them to visibly aggregate. In comparison to a previous multi-step system using aptazymes and gold nanoparticles separately, the present system requires only single handling and no special equipment, making it more suitable for on-the-spot sensing. (C) 2010 Elsevier Ltd. All rights reserved.
  • Atsushi Ogawa, Yasunori Doi, Nobuto Matsushita
    ORGANIC & BIOMOLECULAR CHEMISTRY 9 (24) 8495 - 8503 1477-0520 2011 [Peer-reviewed]
     Scientific journal 
    In vitro-transcribed, unmodified, and non-aminoacylated amber suppressor tRNAs that are recognized by natural aminoacyl-tRNA synthetase were improved toward higher suppression efficiency in batch-mode cell-free translation in wheat germ extract. The suppression efficiency of the suppressor obtained through four sequence optimization steps (anticodon alteration of natural tRNAs (the first generation); chimerization of the efficient suppressors in the first generation; investigation and optimization of the effective parts in the second generation; combination of the optimized parts in the third generation) and by the terminal tuning was approximately 60%, which was 2.4-fold higher than that of the best suppressor in the first generation. In addition, an eRF1 aptamer further increased the efficiency up to 85%. This highly efficient suppression system also functioned well in a dialysis-based large-scale protein synthesis.
  • Atsushi Ogawa
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 20 (20) 6056 - 6060 0960-894X 2010/10 [Peer-reviewed]
     Scientific journal 
    An autonomous DNA machine recycling the output as the input for isothermal, sensitive, and specific detection of miRNAs has been developed. This machine shows considerably high signal amplification efficiency (similar to 1000-fold) and thus a low detection limit (similar to 20 amol). The machine also shows high specificity, discriminating 50 amol of synthetic miRNA from 100-fold larger amounts of its family member and from 100 ng of unrelated total RNAs. Moreover, it is available for practically detecting natural miRNAs in total RNAs. (c) 2010 Elsevier Ltd. All rights reserved.
  • Atsushi Ogawa
    Nucleic acids symposium series 53 261 - 262 2009/12 International conference proceedings 
    I have constructed a novel aptazyme-based biosensor system for detecting cofactors of the aptazymes using a cell-free luciferase synthesis in wheat germ extract. In this system, the activity of the aptazyme that is fused to a 5'-untranslated region of a luciferase gene can be detected as luciferase expression. In translating the aptazyme-fused mRNA as-is using a wheat germ cell-free translation system, the luciferase is almost not expressed because of the following triple suppression effects: (1) 5'-terminal three bases and (2) 5'-terminal duplex prevent the ribosome from binding to own mRNA; (3) if the ribosome binds, translation of a mimic gene in the aptazyme inhibits that of the downstream luciferase gene (OFF state). In contrast, in the presence of the aptazyme cofactor, the aptazyme in mRNA is self-cleaved to produce an aptazyme-free luciferase gene, which is translated efficiently (ON state). The ON/OFF efficiency and the detection limit of the aptazyme-based biosensor for theophylline are much higher and lower, respectively, compared to those of previously-reported one that utilizes a prokaryotic translation system.
  • Atsushi Ogawa
    CHEMBIOCHEM 10 (15) 2465 - 2468 1439-4227 2009/10 [Peer-reviewed]
     Scientific journal 
    (Figure Presented) Sensible wheat germ: I have developed novel biosensors based on a new method for converting aptazyme activity into easily detectable protein expression by translating aptazyme-fused mRNA in eukaryotic wheat germ extract. Some newly elucidated unique features of the wheat germ translation system make the sensitivity of this method much higher than that of previously reported aptazyme-based biosensors. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
  • Atsushi Ogawa, Mizuo Maeda
    CHEMISTRY LETTERS 38 (8) 848 - 849 0366-7022 2009/08 [Peer-reviewed]
     Scientific journal 
    We have developed a detector-free and multiple detection method for various analytes using some orthogonal pairs of cleavase-aptazymes and the corresponding probe-DNA-tethered gold nanoparticles (AuNPs) for each analyte. In this method, each cleavase-aptazyrne cleaves itself only in the presence of its cofactor (i.e. analyte), and each resulting cleaved RNA hybridizes only to the corresponding probe DNA on AuNPs to induce AuNP aggregation that can be detected with the naked eye.
  • Atsushi Ogawa, Mizuo Maeda
    CHEMICAL COMMUNICATIONS (31) 4666 - 4668 1359-7345 2009 [Peer-reviewed]
     Scientific journal 
    We have developed an easy method for constructing aptazyme-based logic gates using noncrosslinking gold nanoparticle aggregation.
  • Atsushi Ogawa, Mizuo Maeda
    Nucleic acids symposium series 52 527 - 528 2008/12 International conference proceedings 
    We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using a unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline dependent aptazyme, 100 microM, 10 microM, and 1 microM theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is applicable to other cleavage-aptazymes without altering the probe-DNA sequence.
  • Atsushi Ogawa, Mizuo Maeda
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 18 (24) 6517 - 6520 0960-894X 2008/12 [Peer-reviewed]
     Scientific journal 
    We developed a method for simply and rapidly detecting cofactors of aptazymes with high sensitivity using unique noncrosslinking gold nanoparticle aggregation. Applying this method to a theophylline-dependent aptazyme, 100 mu M, 10 mu M, and 1 mu M theophylline were detected easily by the naked eye within 10 min, 20 min, and 65 min, respectively. This method is also applicable to other cleavase-aptazymes without altering the probe-DNA sequence on the gold nanoparticle. (C) 2008 Elsevier Ltd. All rights reserved.
  • Atsushi Ogawa, Mizuo Maeda
    CHEMBIOCHEM 9 (14) 2204 - 2208 1439-4227 2008/09 [Peer-reviewed]
     Scientific journal
  • 機能性核酸を用いたターゲット分子簡易検出システムの開発
    小川 敦司, 前田 瑞夫
    日本化学会 生体機能関連化学部会 NEWS LETTER 23 (2) 6 - 9 2008/08 [Invited]
  • Atsushi Ogawa, Mizuo Maeda
    CHEMBIOCHEM 9 (2) 206 - 209 1439-4227 2008/01 [Peer-reviewed]
     Scientific journal
  • Atsushi Ogawa, Mizuo Maeda
    Nucleic acids symposium series 51 389 - 390 2007/12 International conference proceedings 
    We constructed a new-type riboswitch, which functions in E. coli, using an aptazyme and an anti-RBS sequence. This riboswitch usually suppresses the gene expression with its anti-RBS sequence bound to the RBS of its own mRNA(OFF), while it activates the translation only when a cofactor of the aptazyme is added to release the anti-RBS sequence from itself as a result of cofactor-induced self-cleavage by the aptazyme (ON). Although this aptazyme-based riboswitch did not function at 37 degrees C in vivo in spite of its high activity at this temperature in vitro, it worked well at lower temperature (23 degrees C). We also improved the efficiency of this riboswitch by constructing a cascading system.
  • Atsushi Ogawa, Mizuo Maeda
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 17 (11) 3156 - 3160 0960-894X 2007/06 [Peer-reviewed]
     Scientific journal 
    We constructed a label-free and detector-free aptazyme-based riboswitch sensor for detecting the cofactor of the aptazyme. This riboswitch, which usually suppresses the gene expression with its anti-RBS sequence bound to the RBS of its own mRNA (OFF), activates the translation only when a cofactor is added to release the anti-RBS sequence from itself as a result of cofactor-induced self-cleavage by the aptazyme (ON). The rationally optimized one with beta-galactosidase as a reporter gene enabled us to detect the cofactor of the aptazyme visibly with high ON/OFF efficiency. (C) 2007 Elsevier Ltd. All rights reserved.
  • Shinsuke Sando, Atsushi Ogawa, Teruyuki Nishi, Masayoshi Hayami, Yasuhiro Aoyama
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 17 (5) 1216 - 1220 0960-894X 2007/03 [Peer-reviewed]
     Scientific journal 
    A pool of 84-nt RNAs containing a randomized sequence of 50 nt was selected against gel-immobilized Escherichia coli release factor 1 (RF-1) responsible for translation termination at amber (UAG) stop codon. The strongest aptamer (class II-I) obtained from 43 clones bound to RF-1, but not to UAA/UGA-targeting RF-2, with K-d = 30 +/- 6 nM (SPR). A couple of impaired hairpin domains in the aptamer were suggested as the sites of attachment of RF-1. By binding to and hence inhibiting the action of RF-1 specifically or bio-orthogonally, aptamer class II-I enhanced the amber suppression efficiency in the presence of an anticodon-adjusted (CUA) suppressor tRNA without practically damaging the protein translation machinery of the cell-free extract of E coli, as confirmed by the translation of amber-mutated (gfp(amber141) or gfp(amber178)) and wild-type (gfp(wild)) genes of GFP. (c) 2006 Elsevier Ltd. All rights reserved.
  • A Ogawa, S Sando, Y Aoyama
    CHEMBIOCHEM 7 (2) 249 - 252 1439-4227 2006/02 [Peer-reviewed]
     Scientific journal 
    Don't stop me now. Anticodon-adjusted tRNASer can be used as unified suppressors of the three types of stop codon. In their presence, translation of pseudonatural mRNAs for DHFR is rendered termination-free and proceeds into the untranslated region (UTR), giving rise to protein-ribosome- mRNA complex with full display of the protein when the translated UTR peptide serving as a spacer arm has a chain-length of ≥50 amino acids (aa). The mRNA template is recovered from the tag-selected complex with a 74-aa UTR in a yield of 2-4% based on input mRNA. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
  • Atsushi Ogawa, Teruyuki Nishi, Shinsuke Sando, Yasuhiro Aoyama
    Nucleic acids symposium series 49 269 - 270 2005/12 International conference proceedings 
    We carried out an in vitro selection of RNA aptamers that bind to Escherichia coli release factor 1 (E. coli RF1). The selected aptamer (class II) showed an apparent dissociation constant of nM range. The binding of the class II aptamer with E. coli RF1 is highly specific (orthogonal), allowing selective inhibition of RF1 activity in the E. coli translation system.
  • Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    Nucleic acids symposium series 49 267 - 268 2005/12 International conference proceedings 
    Combination of nonsense suppression and protein-ribosome-mRNA (PRM) complexation techniques leads to a new strategy "read-through polysome/ribosome display", which is designed to display full-length open reading frame (ORF) domain of the protein on the natural mRNA templates. The optimised conditions are to use the anticodon-adjusted tRNA for Leu as a nonsense suppressor in a reconstituted translation system containing diminished amounts of release factors (RFs). When applied to pseudo-natural mRNAs of Escherichia coli dihydrofolate reductase (E. coli DHFR), the input mRNA was recovered as a polysome complex displaying full-length DHFR.
  • T Ohwada, H Hirao, A Ogawa
    JOURNAL OF ORGANIC CHEMISTRY 69 (22) 7486 - 7494 0022-3263 2004/10 [Peer-reviewed]
     Scientific journal 
    It has been experimentally established that the proton affinities (PA), as well as the solution basicities (pK(BH)(+)), of aziridine derivatives are much smaller than those of the corresponding pyrrolidines and piperidines, though the basic strength of azetidines is close to those of pyrrolidines and piperidines. A simple idea of dependence of the basic strength on bond angles seems to be invalid. Because the basicity of cyclic amines is a fundamental property in organic chemistry, we revisited this topic in order to clarify quantitatively the intrinsic origin of the strength of Lewis basicity of the relevant amines, in particular, based on the local electron-donating ability of the amine nitrogen atoms evaluated in terms of the localized reactive hybrid orbital (RHO) concept. In the cases of representative N-substituents such as hydrogen, methyl, and phenyl groups, the electron-donating energy level of the nitrogen center, obtained by maximizing a kind of superdelocalizability, was shown to be correlated with the magnitudes of experimental and calculated gas-phase proton affinities. The present results strongly support the view that the C-N-C bond angle, i.e., angle strain, in the cyclic amines is not the major source of the difference in strength of basicity of these amines, but rather, the degree of pyramidalization around the nitrogen atom has a significant impact on the electron-donating ability of the nitrogen lone-pair orbital.
  • A Ogawa, N Tomita, N Kikuchi, S Sando, Y Aoyama
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 14 (15) 4001 - 4004 0960-894X 2004/08 [Peer-reviewed]
     Scientific journal 
    An affinity column immunobilizing a decapeptide H2N-RGDSPASSKP-CO2H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion. (C) 2004 Elsevier Ltd. All right, reserved.
  • A Ogawa, M Tachibana, M Kondo, K Yoshizawa, H Fujimoto, R Hoffmann
    JOURNAL OF PHYSICAL CHEMISTRY B 107 (46) 12672 - 12679 1520-6106 2003/11 [Peer-reviewed]
     Scientific journal 
    C-Cu orbital interactions between a two-layer Cu-10 or three-layer Cu-34 cluster model of a Cu(111) surface and an adsorbed single C-60 molecule have been theoretically investigated, so as to elucidate the nature of the C-60-Cu(111) bonding and orientational configuration of the C-60 molecule on a Cu surface. Geometry optimizations and single-point calculations at the B3LYP/LanL2MB level of theory and fragment molecular orbital (FMO) analyses, coupled with a paired-interaction-orbital (PIO) scheme at the extended Hackel level of theory, have been performed for five symmetric adsorption models, in which a C-60 molecule is attached to the Cu-10 or Cu-34 cluster respectively by a six-membered ring (6-ring), by a five-membered ring (5-ring), by a C-C bond belonging to two 6-rings (6-6 bond), by a C-C bond belonging to a 6-ring and a 5-ring (5-6 bond), and by an edge carbon atom that is located at the center of two 6-rings and a 5-ring. Large stabilization is obtained for adsorption by an edge carbon atom or a 6-6 bond, whereas the other coordination types are not favored. Our result differs from an XPD experimental result for a C-60 monolayer on Cu(111), in which adsorption by a 6-ring is most favored. The discrepancy strongly suggests that C-60-C-60 interactions contribute significantly to the determination of C-60 orientations in C-60/Cu(111) monolayer systems.
  • C Dohno, A Ogawa, K Nakatani, Saito, I
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 125 (34) 10154 - 10155 0002-7863 2003/08 [Peer-reviewed]
     Scientific journal 
    Recent studies predict that adenine radical cation (A•+) contributes to the hole-trapping process through long A/T sequences and exists as a real chemical intermediate. However, the experimental evidence for the existence of A•+ has not been observed in the DNA-mediated hole transport reaction. To examine the direct contribution of A•+, we have developed a novel hole-trapping nucleobase NA6-cyclopropyldeoxyadenosine (dCPA) which possesses a cyclopropyl group as a radical trapping device. One-electron oxidation of dCPA revealed that dCPA radical cation undergoes a rapid cyclopropane ring opening. With the use of the dCPA-containing DNA, we have demonstrated that the migrating hole was trapped at CPA incorporated into a long A/T bridge between two GG sites. The present results indicate that nucleobases possessing ionization potential higher than that of dG, such as dA, are able to participate directly in the multistep hopping mechanism. Copyright © 2003 American Chemical Society.
  • M Tachibana, K Yoshizawa, A Ogawa, H Fujimoto, R Hoffmann
    JOURNAL OF PHYSICAL CHEMISTRY B 106 (49) 12727 - 12736 1520-6106 2002/12 [Peer-reviewed]
     Scientific journal 
    With the aim of understanding the nature of the S -Au(111) bonding in organosulfur/Au(111) self-assembled monolayer (SAM) systems, orbital interactions in the adsorption of methanethiolate (-SCH3) in various binding sites of a three-layer slab model and an Au-42 Cluster model of Au(111) surface are investigated. The methods of choice are crystal orbital overlap population (COOP) and crystal orbital Hamilton population (COHP) analyses for a periodic slab model and fragment molecular orbital (FMO) analyses for the cluster model. The origin of the S -Au(111) bond and the binding site preference are discussed from the viewpoint of orbital interaction. The site preference is in the order of three-fold hollow (fcc and hcp) > bridge > on-top. The second layer Au atoms have little influence on the S-Au(111) bonding, and adsorptions, to the fcc and hcp, sites are almost identical with respect to energy and S-Au bonding nature. Although sigma-type S-Au orbital interactions dominate the S-Au(111) bonding in the on-top model,pi-type S-Au orbital interactions play an important role in the bridge, fcc, and hcp models. FMO results,explain the vertical S-C bonds in the hollow models and the tilted S-C bonds in the on-top and bridge models.
  • S Matsubara, K Ukai, H Fushimi, Y Yokota, H Yoshino, K Oshima, K Omoto, A Ogawa, Y Hioki, H Fujimoto
    TETRAHEDRON 58 (41) 8255 - 8262 0040-4020 2002/10 [Peer-reviewed]
     Scientific journal 
    A reaction of 1,2-diketone with bis(iodozincio)methane gave a cyclopropanediol derivative via [2+1] cycloaddition. The reaction proceeded via a sequential nucleophilic attack of the dizinc reagent to a couple of carbonyl group in the substrate. The reaction proceeded with high diastereoselectivity to give cis-isomer. Detailed mechanistic studies have been examined by ab initio calculation. (C) 2002 Elsevier Science Ltd. All rights reserved.
  • A Ogawa, H Fujimoto
    INORGANIC CHEMISTRY 41 (19) 4888 - 4894 0020-1669 2002/09 [Peer-reviewed]
     Scientific journal 
    The Lewis acidity of GaF3, GaF2Cl, GaFCl2, and GaCl3 in acid-base interactions has been studied by taking ammonia as their electron-donating counterpart. We have derived an unoccupied reactive orbital that shows the maximum localization on the Ga atomic center for each species. The orbital is located lower in energy compared to those in the corresponding boron and aluminum halides. In contrast to boron halides, the unoccupied reactive orbital of the acid site tends to be delocalized considerably on the halogens as the fluorines are substituted by chlorines in gallium halides. The trend observed in the effects of fluorine and chlorine on the acidity of the gallium halides is opposite to those found in the boron halides. This cannot be interpreted solely in terms of the electron-accepting strength of the gallium center, but can be understood by including electrostatic interactions and closed-shell repulsion with ammonia in the adducts. The origin of the difference in Lewis acidity Of BCl3, AlCl3, and GaCl3 has been clarified.
  • A Ogawa, H Fujimoto
    TETRAHEDRON LETTERS 43 (11) 2055 - 2057 0040-4039 2002/03 [Peer-reviewed]
     Scientific journal 
    An application of the perturbation theory to the [4+2] addition of butadiene has demonstrated that the effect of secondary orbital interactions should be much less significant than has been assumed within the frontier orbital scheme. This has been confirmed by a numerical analysis with respect to the endo transition state of the Diels-Alder reaction between butadiene and maleic anhydride. (C) 2002 Elsevier Science Ltd. All rights reserved.
  • Kazuhiko Nakatani, Chikara Dohno, Atsushi Ogawa, Isao Saito
    Chemistry and Biology 9 (3) 361 - 366 1074-5521 2002 [Peer-reviewed]
     Scientific journal 
    A guanine radical cation produced by one-electron DNA oxidation migrates over long distances through the DNA π-stack. Fundamental questions regarding the likelihood of charge transport in genomic DNA, the effects of protein binding, and its biological consequences arise as the next issues of study. Electronic effects of protein binding on the efficiency of charge transport were investigated for the endonuclease BamHI-DNA complex. Direct contact of a positively charged guanidium group of BamHI to guanines in the recognition sequence 5′-GGATCC-3′ completely suppressed one-electron oxidation of the guanine in the protein binding site and dramatically lowered the charge transport efficiency through the sequence. Electronically insulated guanines, by the hydrogen bonding contact of a guanidium group in BamHI, no longer function as a stepping stone in the charge transport through the DNA π-stack.

Books etc

  • Applied RNA Bioscience
    Atsushi Ogawa (Contributor, Chapter6 "Rational Design of Artificial Riboswitches")
    Springer 2018/05 9789811083716 286 79-93
  • Methods in Molecular Biology: Artificial Riboswitches
    Atsushi Ogawa (Editor)
    Springer 2014/03 9781627037556 289
  • 金ナノテクノロジー -その基礎と応用-
    小川 敦司, 前田 瑞夫 (Contributor, 第23章「金ナノ粒子を用いるバイオセンシング」)
    シーエムシー出版 2009/03 9784781300917 345 294-308

Conference Activities & Talks

  • Development of human CBF1-targeting single-stranded DNA aptamers as angiogenic inhibitors
    Mari Kagajo, Yoshiko Hatta, Masashi Maekawa, Atsushi Ogawa, Minenori Ishimae, Eiichi Ishii, Mariko Eguch, Shigeki Higashiyama
    第79回日本癌学会学術総会(Web配信)  2020/10
  • 血管新生を阻害するCBF1-single stranded DNAアプタマーの開発  [Not invited]
    加賀城 真理, 八田 佳子, 前川 大志, 小川 敦司, 石井 榮一, 江口 真理子, 東山 繁樹
    第61回生化学会中国・四国支部例会(誌上発表)  2020/07
  • 2種の核酸アプタマー修飾ナノ粒子の架橋による混合色輝点の形成を利用したトロンビン検出
    吉村健, 矢野湧暉, 矢野雄暉, 小川敦司, 前田瑞夫, 朝日剛, 齋藤伸吾, 吉本敬太郎, 座古保
    第80回分析化学討論会(誌上発表)  2020/05
  • リボスイッチ媒介化学コミュニケーション型の真核系超巨大人工細胞  [Not invited]
    髙橋 萌, 小川 敦司
    「細胞を創る」研究会12.0  2019/10
  • ナノDNA応答性真核系人工リボスイッチの構築  [Not invited]
    伊藤 優, 小川 敦司
    「細胞を創る」研究会12.0  2019/10
  • 異なる2種のナノ粒子の架橋による混合色輝点の形成を利用したトロンビン検出  [Not invited]
    吉村 健, 矢野 湧暉, 矢野 雄暉, 小川 敦司, 前田 瑞夫, 朝日 剛, 齋藤 伸吾, 吉本 敬太郎, 座古 保
    日本分析化学会第68年会  2019/09
  • Gene expression in a super-giant liposome encapsulating a wheat germ extract  [Not invited]
    Hajime Takahashi, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2019  2019/09
  • 高密度固定化DNAを介した核酸アプタマー修飾金ナノ粒子を用いた夾雑試料中からの分子検出  [Not invited]
    矢野 雄暉, 矢野 湧暉, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    日本分析化学会第68年会  2019/09
  • マルチターンオーバー型真核系リボスイッチの開発  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2019(第14 回)  2019/06
  • 固定化DNAを介して核酸アプタマーを修飾した金ナノ粒子によるトロンビンタンパク質の検出  [Not invited]
    吉村 健, 矢野 湧暉, 矢野 雄暉, 小川 敦司, 前田 瑞夫, 朝日 剛, 古性 均, 齋藤 伸吾, 吉本 敬太郎, 座古 保
    第79回分析化学討論会  2019/05
  • 固定化DNAを介した核酸アプタマー修飾金ナノ粒子を用いた選択的分子検出の検討  [Not invited]
    矢野 雄暉, 矢野 湧暉, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    第79回分析化学討論会  2019/05
  • Development of a strictly controlled inducible gene expression system for plastids by using synthetic riboswitches  [Not invited]
    Rika Yamane, Atsushi Ogawa, Yuzuru Tozawa, Takashi Shiina, Yoichi Nakahira
    Gordon Research Conference (Chloroplast Biotechnology 2019)  2019/01
  • 人工リボスイッチを基盤とした葉緑体遺伝子発現誘導系の改良  [Not invited]
    山根 里佳, 小川 敦司, 戸澤 譲, 椎名 隆, 中平 洋一
    第41回日本分子生物学会年会  2018/11
  • 金ナノ粒子凝集を用いたトロンビン検出におけるアプタマーの影響評価  [Not invited]
    吉村 健, 矢野 湧暉, 矢野 雄暉, 小川 敦司, 前田 瑞夫, 古性 均, 齋藤 伸吾, 吉本 敬太郎, 座古 保
    2018年日本化学会中国四国支部大会  2018/11
  • 暗視野イメージング画像の色分解による標的分子検出に向けた夾雑系での金ナノ粒子輝点解析法  [Not invited]
    矢野 湧暉, 矢野 雄暉, 二艘木 優充, 中西 文香, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    2018年日本化学会中国四国支部大会  2018/11
  • 標的分子の選択的検出を目的とした金ナノ粒子表面修飾の検討  [Not invited]
    矢野 雄暉, 矢野 湧暉, 小川 敦司, 朝日 剛, 前田 瑞夫, 座古 保
    2018年日本化学会中国四国支部大会  2018/11
  • 小麦無細胞系におけるRNA3’末端保護配列のin vitro selection  [Not invited]
    忽那 茜, 奥園 達也, 高松 将史, 小川 敦司
    2018年日本化学会中国四国支部大会  2018/11
  • 鎖置換反応を必要としないIRES基盤真核系人工OFFリボスイッチの開発  [Not invited]
    升岡 宏紀, 太田 翼, 髙橋 萌, 小川 敦司
    2018年日本化学会中国四国支部大会  2018/11
  • 省エネ型・IRES基盤真核系人工OFFリボスイッチの開発  [Not invited]
    升岡 宏紀, 太田 翼, 髙橋 萌, 小川 敦司
    第13回無細胞生命科学研究会  2018/10
  • コムギ無細胞系におけるmRNA安定化および翻訳効率上昇を指向した3’末端保護配列のin vitro selection  [Not invited]
    忽那 茜, 奥園 達也, 高松 将史, 小川 敦司
    第13回無細胞生命科学研究会  2018/10
  • 選択的バイオセンシングのための金ナノ粒子表面修飾の検討  [Not invited]
    矢野 雄暉, 矢野 湧暉, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    日本分析化学会第67年会  2018/09
  • 暗視野イメージング画像の色分解による標的分子検出に向けた金ナノ粒子凝集解析法  [Not invited]
    矢野 湧暉, 二艘木 優充, 矢野 雄暉, 中西 文香, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    日本分析化学会第67年会  2018/09
  • In vitro selection of a 3’ protector that stabilizes mRNA to increase the translation efficiency in a wheat germ cell-free expression system  [Not invited]
    Akane Kutsuna, Tatsuya Okuzono, Masashi Takamatsu, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2018  2018/09
  • Artificial OFF-riboswitches that downregulate internal ribosome entry without hybridization switches in a wheat germ extract  [Not invited]
    Hiroki Masuoka, Tsubasa Ota, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2018  2018/09
  • EVALUATION OF THEOPHYLLINE-DEPENDENT SYNTHETIC RIBOSWITCHES FOR STRICT CONTROL OF PLASTID GENE EXPRESSION  [Not invited]
    Rika Yamane, Atsushi Ogawa, Yuzuru Tozawa, Takashi Shiina, Yoichi Nakahira
    INTERNATIONAL PLANT MOLECULAR BIOLOGY 2018  2018/08
  • 暗視野イメージング下での金ナノ粒子の散乱分光による標的分子の検出  [Not invited]
    矢野 湧暉, 二艘木 優充, 矢野 雄暉, 中西 文香, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    第78回分析化学討論会  2018/05
  • 暗視野顕微鏡を利用したナノ粒子凝集体の観察による有色サンプル中での分子検出  [Not invited]
    矢野 雄暉, 矢野 湧暉, 小川 敦司, 前田 瑞夫, 朝日 剛, 座古 保
    第78回分析化学討論会  2018/05
  • 人工リボスイッチを活用した葉緑体遺伝子発現誘導系の評価  [Not invited]
    山根 里佳, 小川 敦司, 戸澤 譲, 椎名 隆, 中平 洋一
    第40回日本分子生物学会年会  2017/12
  • Ligand-responsive upregulation of 3' CITE-mediated translation in wheat germ extract  [Not invited]
    Yuta Murashige, Junichiro Tabuchi, Taiki Omatsu, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2017  2017/09
  • Rational design of artificial riboswitches that function in a eukaryotic expression system  [Invited]
    Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2017  2017/09
  • 金ナノ粒子凝集の単一クラスター解析を用いた疾病マーカーmiRNAの高感度検出  [Not invited]
    矢野 湧暉, 二艘木 優充, 小川 敦司, 朝日 剛, 前田 瑞夫, 座古 保
    日本分析化学会第66年会  2017/09
  • 人工リボスイッチを活用した葉緑体遺伝子発現制御系の評価  [Not invited]
    山根 里佳, 小川 敦司, 戸澤 譲, 椎名 隆, 中平 洋一
    第35回日本植物細胞分子生物学会  2017/08
  • 暗視野顕微鏡を用いた金ナノ粒子凝集の単一クラスター解析によるマイクロRNAの高感度検出  [Not invited]
    矢野 湧暉, 二艘木 優充, 小川 敦司, 朝日 剛, 前田 瑞夫, 座古 保
    第77回分析化学討論会  2017/05
  • 人工リボスイッチを基盤とした葉緑体遺伝子発現誘導系  [Not invited]
    中平 洋一, 山根 里佳, 小川 敦司, 戸澤 譲, 椎名 隆
    第39回日本分子生物学会年会  2016/12
  • 鎖置換を利用しない真核系人工ONリボスイッチの合理設計  [Not invited]
    村重 裕太, 田渕 潤一郎, 大松 大希, 小川 敦司
    「細胞を創る」研究会9.0  2016/11
  • Facile design of artificial riboswitches that ligand-dose dependently upregulate translation without hybridization switches in a wheat germ extract  [Not invited]
    Yuta Murashige, Junichiro Tabuchi, Taiki Omatsu, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2016  2016/09
  • アプタマー修飾金ナノ粒子凝集の単一クラスター観察による高感度分子検出  [Not invited]
    座古 保, 矢野 湧暉, 大國 烈, 二艘木 優充, 前田 瑞夫, 小川 敦司, 朝日 剛
    日本分析化学会 第65年会  2016/09
  • 人工リボスイッチを用いたタバコ葉緑体遺伝子発現誘導系  [Not invited]
    中平 洋一, 小川 敦司, 戸澤 譲, 椎名 隆
    第34回日本植物細胞分子生物学会  2016/09
  • 省エネ型真核リボスイッチの設計  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2016  2016/06
  • 金ナノ粒子凝集の一分子観察による、タンパク質高感度検出  [Invited]
    座古 保, 大國 烈, 二艘木 優充, 前田 瑞夫, 小川 敦司, 朝日 剛
    第76回分析化学討論会  2016/05
  • Utilization of the theophylline-dependent engineered riboswitches for strict control of plastid gene expression in tobacco  [Not invited]
    中平 洋一, 小川 敦司, 戸澤 譲, 椎名 隆
    第57回植物生理学会年会  2016/03
  • Rational engineering of ribosomal shunt-modulating eukaryotic ON-riboswitches  [Not invited]
    Atsushi Ogawa
    Pacifichem 2015  2015/12
  • Riboswitch制御でのシアノファージ由来Sigma factorによるシアノバクテリアの糖代謝改変  [Not invited]
    沢 稔彦, 小川 敦司, 田附 常幸, 廣川 安孝, 小山 内崇, 花井 泰三
    第22回日本生物工学会九州支部宮崎大会  2015/12
  • コムギ胚芽抽出液中におけるtRNA末端processingを利用したaptamer基盤センサー(aptasensor)の開発  [Not invited]
    田渕 潤一郎, 小川 敦司
    BMB 2015  2015/12
  • Biofunction-assisted aptasensors based on ligand-dependent 3' processing of a suppressor tRNA in a wheat germ extract  [Not invited]
    Junichiro Tabuchi, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2015  2015/09
  • RNAプロセシングを利用したシュードリボスイッチの開発  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2015  2015/06
  • Identification of short untranslated regions that sufficiently enhance translation in wheat germ extract  [Not invited]
    Junichiro Tabuchi, Yasunori Doi, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2014  2014/09
  • コムギ胚芽抽出液中で高い翻訳促進効果を発揮する短い非翻訳領域  [Not invited]
    田渕 潤一郎, 土居 靖典, 小川 敦司
    第8回バイオ関連化学シンポジウム  2014/09
  • Hybridization switch-freeの真核系ONリボスイッチを創る  [Not invited]
    小川 敦司
    第8回バイオ関連化学シンポジウム  2014/09
  • 分子応答性RNAプロセシングシステムの開発  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2014  2014/06
  • 人工リボスイッチを基盤としたラン藻の新規遺伝子発現制御技術  [Not invited]
    中平 洋一, 小川 敦司, 浅野 宏幸, 小山 時隆, 戸澤 譲
    第55回日本植物生理学会年会  2014/03
  • 小麦胚芽無細胞翻訳システムを利用した真核系人工リボスイッチの開発  [Invited]
    小川 敦司
    第1回合成生物学研究部会セミナー  2014/03
  • 人工リボスイッチを用いたラン藻のための新規遺伝子発現制御技術  [Not invited]
    中平 洋一, 小川 敦司, 浅野 宏幸, 小山 時隆, 戸澤 譲
    第36回日本分子生物学会年会  2013/12
  • Theophylline-dependent Riboswitch as a Useful Genetic Tool for Synthetic Biology in Cyanobacteria  [Not invited]
    Yoichi Nakahira, Atsushi Ogawa, Hiroyuki Asano, Tokitaka Oyama, Yuzuru Tozawa
    CBI Annual Meeting 2013  2013/10
  • t-Riboregulator: Regulation of Nonsense Suppression by Modulating 3' Processing of Suppressor tRNA  [Not invited]
    Yasunori Doi, Atsushi Ogawa
    CBI Annual Meeting 2013  2013/10
  • 真核系無細胞翻訳システムを利用したshunting基盤人工リボスイッチの開発  [Not invited]
    小川 敦司
    新学術領域「合成生物学」:第4回領域全体会議  2013/10
  • Theophylline-dependent synthetic riboswitch is a useful genetic tool for strict regulation of protein expression in cyanobacteria  [Not invited]
    Yoichi Nakahira, Atsushi Ogawa, Hiroyuki Asano, Tokitaka Oyama, Yuzuru Tozawa
    Protein Island Matsuyama International Symposium 2013  2013/09
  • Assessment and Applications of tRNA processing in Wheat Germ Extract by Using a Nonsense Suppression System  [Not invited]
    Yasunori Doi, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2013  2013/09
  • コムギ無細胞システムを利用した真核リボスイッチの合理設計  [Invited]
    小川 敦司
    第15回日本RNA学会年会  2013/07
  • 無細胞翻訳システムを利用した真核系人工リボスイッチの開発  [Invited]
    小川 敦司
    第190回応用化学セミナー  2013/06
  • ラン藻のための新規遺伝子発現制御技術の開発  [Not invited]
    中平 洋一, 小川 敦司, 淺野 宏幸, 小山 時隆, 戸澤 譲
    第54回日本植物生理学会年会  2013/03
  • IRES依存翻訳を利用した真核系リボスイッチの合理設計  [Not invited]
    小川 敦司
    第7回無細胞生命科学研究会  2012/11
  • 小麦胚芽抽出液中で働くsuppressor-tRNAの人工進化  [Not invited]
    土居 靖典, 松下 修門, 小川 敦司
    第7回無細胞生命科学研究会  2012/11
  • Evaluation and Regulation of tRNA Processing by Using Terminal-Premature Suppressor tRNAs in Wheat Germ Extract  [Not invited]
    Yasunori Doi, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2012  2012/09
  • Rational Design of IRES-Based Eukaryotic ON-Riboswitches  [Not invited]
    Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2012  2012/09
  • 合成生物学基盤技術を指向した人工リボスイッチの開発  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2012  2012/06
  • 真核系無細胞翻訳システムを利用したShunting基盤人工リボスイッチの開発  [Not invited]
    小川 敦司
    新学術領域「合成生物学」:第3回領域全体会議  2012/05
  • Artificial Evolution of Unmodified Suppressor tRNAs toward Higher Suppression Efficiency in Wheat Germ Extract  [Not invited]
    Yasunori Doi, Nobuto Matsushita, Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2011  2011/09
  • IRESを用いた真核系リボスイッチの合理的設計  [Not invited]
    小川 敦司
    第5回バイオ関連化学シンポジウム  2011/09
  • 小麦胚芽抽出液中で働くsuppressor-tRNAの人工進化  [Not invited]
    土居 靖典, 松下 修門, 小川 敦司
    第5回バイオ関連化学シンポジウム  2011/09
  • 真核生物の翻訳系で働く「分子応答性遺伝子発現制御システム」の合理的設計  [Not invited]
    小川 敦司
    第10回バイオテクノロジー国際会議  2011/07
  • 無細胞翻訳システムを利用した人工リボスイッチの開発  [Invited]
    小川 敦司
    生体機能関連化学部会若手の会・第23回サマースクール  2011/07
  • 無細胞生命システムを利用した新しいバイオテクノロジーの開発  [Invited]
    小川 敦司
    第4回稲盛フロンティア研究セミナー  2011/04
  • Biofunction-assisted sensors based on artificial riboswitches in wheat germ extract  [Not invited]
    Atsushi Ogawa
    Pacifichem 2010  2010/12
  • Controlling Salt Resistance of Oligonucleotide-tethered AuNPs with Their Diameter for Their Electrosteric Stability-based Visible Sensing Systems  [Not invited]
    Atsushi Ogawa
    The 7th International symposium on Nucleic Acid Chemistry  2010/11
  • Development of New Biotechnologies Utilizing Biomolecules and Biofunctions  [Invited]
    小川 敦司
    24th Luncheon Seminar  2010/10
  • 小麦胚芽の無細胞翻訳システムを用いたリボスイッチ型・アプタザイム基盤バイオセンサー  [Not invited]
    小川 敦司
    第5回無細胞生命科学研究会  2010/09
  • Aptazyme-Based Biosensors Utilizing Wheat Germ Extract  [Not invited]
    Atsushi Ogawa
    Protein Island Matsuyama International Symposium 2010  2010/09
  • 無細胞生命システムを利用したニューバイオテクノロジー  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2010  2010/05
  • 真核生物の無細胞翻訳システムを用いたアプタザイム基盤バイオセンサー  [Not invited]
    小川 敦司
    日本化学会第90春季年会  2010/03
  • Aptazyme-Based Biosensors Using a Eukaryotic Cell-Free Translation System  [Not invited]
    Atsushi Ogawa
    The 6th International symposium on Nucleic Acid Chemistry  2009/09
  • 無細胞翻訳システムを用いた新しいバイオテクノロジーの開発  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2009  2009/05
  • Molecule-Dependent Gene Regulation  [Invited]
    小川 敦司
    3rd Luncheon Seminar  2009/04
  • アプタザイム基盤人工リボスイッチの開発  [Not invited]
    小川 敦司, 前田 瑞夫
    日本化学会第89春季年会  2009/03
  • 分子応答性タンパク質発現システムの開発  [Not invited]
    小川 敦司, 前田 瑞夫
    第3回無細胞生命科学研究会  2009/03
  • 機能性核酸アプタザイムを用いた新規遺伝子発現制御法・分子検出法の開発  [Invited]
    小川 敦司
    第37回環境分子科学研究会  2008/10
  • Simple and Rapid Colorimetric Detection of a Cofactor of an Aptazyme Using Noncrosslinking Gold Nanoparticle Aggregation  [Not invited]
    Atsushi Ogawa, Mizuo Maeda
    Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th International Symposium on Nucleic Acids Chemistry  2008/09
  • 非架橋型金ナノ粒子凝集反応を用いた小分子の迅速・簡便検出  [Invited]
    小川 敦司, 前田 瑞夫
    第57回高分子討論会  2008/09
  • サプレッサーtRNA基盤リボスイッチシステムの開発  [Not invited]
    小川 敦司, 前田 瑞夫
    第3回バイオ関連化学合同シンポジウム  2008/09
  • アプタザイムを利用した小分子検出システムの開発  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2008  2008/06
  • 機能性核酸を用いた小分子検出および遺伝子発現制御  [Invited]
    小川 敦司
    京都大学青山研セミナー  2008/06
  • Development of a New-type Riboswitch Using an Aptazyme and an anti-RBS Sequence  [Not invited]
    Atsushi Ogawa, Mizuo Maeda
    5th International Symposium on Nucleic Acids Chemistry  2007/11
  • Aptazyme-based riboswitches  [Not invited]
    Atsushi Ogawa, Mizuo Maeda
    234th ACS National Meeting  2007/08
  • 新規リボスイッチシステムの開発とその応用  [Not invited]
    小川 敦司
    新世代の生物有機化学研究会2007  2007/06
  • 蛋白質翻訳システムの応用に向けた化学的アプローチ  [Not invited]
    益 啓貴, 速水 将勝, 小川 敦司, 西 輝之, 山東 信介, 青山 安宏
    日本ケミカルバイオロジー研究会第1回年会  2007/05
  • 蛋白質翻訳システムの応用に向けた化学的アプローチ  [Not invited]
    益 啓貴, 速水 将勝, 小川 敦司, 西 輝之, 山東 信介, 青山 安宏
    日本化学会第87回春季年会  2007/03
  • アプタザイムを利用した新規リボスイッチの開発  [Not invited]
    小川 敦司, 前田 瑞夫
    日本化学会第87回春季年会  2007/03
  • Read-Through Polysome/Ribosome Display of Full-Length Protein ORF Using Pseudo-Native mRNAs with a 3'-Untranslated Region (UTR) as a Buried Spacer Arm  [Not invited]
    Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    Pacifichem 2005  2005/12
  • 大腸菌解離因子(RF1)に特異的に結合するRNAアプタマーのIn vitro selection  [Not invited]
    西 輝之, 小川 敦司, 速水 将勝, 山東 信介, 青山 安宏
    第20回生体機能関連化学シンポジウム  2005/09
  • In vitro selection of RNA aptamers for the Escherichia coli release factor 1  [Not invited]
    Atsushi Ogawa, Teruyuki Nishi, Shinsuke Sando, Yasuhiro Aoyama
    4th International Symposium on Nucleic Acids Chemistry  2005/09
  • In vitro Read-Through Polysome/Ribosome Display of Full-Length Protein ORF and it’s Applications  [Not invited]
    Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    4th International Symposium on Nucleic Acids Chemistry  2005/09
  • In vitroでsuppressor tRNAをセレクションする  [Not invited]
    小川 敦司, 山東 信介, 青山 安宏
    第20回生体関連化学シンポジウム  2005/09
  • Ribosome Display in the compartmentalized reversed phase micelle  [Not invited]
    Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    Anglo-German-Japanese Biochemistry Meeting  2005/09
  • Read-through ribosome/polysome display and its application to the in vitro selection of suppressor tRNAs  [Not invited]
    Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    230th ACS National Meeting  2005/08
  • 天然mRNAを鋳型とする全長タンパク質ディスプレイ法の開発  [Not invited]
    小川 敦司, 山東 信介, 青山 安宏
    遺伝子・デリバリー研究会5周年記念国際シンポジウム  2005/05
  • 天然mRNAを鋳型とする全長タンパク質ディスプレイ法の開発  [Not invited]
    小川 敦司, 山東 信介, 青山 安宏
    COE拠点領域(生体関連物質化学)融合集中講義  2005/02
  • プロテインコードを拡張する:蛋白のグリコシル化を触媒するリボザイム  [Not invited]
    小川 敦司, 菊地 直子, 山東 信介, 青山 安宏
    日本化学会第84回春季年会  2004/03
  • 機能性オリゴヌクレオチド:アプタマー、リボザイムの進化と機能獲得  [Not invited]
    小川 敦司, 菊地 直子, 富田 尚利, 山東 信介, 青山 安宏
    COE拠点領域(生体関連物質化学)融合集中講義  2003/12
  • Short ODN inhibits cell-adhesion  [Not invited]
    Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    The 4th International Forum on Chemistry of Functional Organic Chemicals (IFOC-4)  2003/11
  • DNAが細胞接着を阻害する  [Not invited]
    小川 敦司, 山東 信介, 青山 安宏
    第1回生体機能関連・バイオテクノロジー合同シンポジウム  2003/10
  • Short ODN inhibits cell-adhesion  [Not invited]
    Atsushi Ogawa, Shinsuke Sando, Yasuhiro Aoyama
    Japanese-German Biochemistry Meeting  2003/09
  • 銅(111)表面とそれに吸着するフラーレン分子間の軌道相互作用解析  [Not invited]
    小川 敦司, 立花 正満, 吉澤 一成, 藤本 博
    分子構造総合討論会2002  2002/10
  • Au(111)表面上のアルカンチオレート自己組織化単分子膜におけるS-Au結合に関する理論的研究  [Not invited]
    立花 正満, 吉澤 一成, 小川 敦司, 藤本 博, Roald Hoffmann
    日本化学会第81春季年会  2002/03
  • ジベンゾビシクロ[2.2.2]オクタトリエンのDiels-Alder反応における面選択性についての理論的研究  [Not invited]
    小川 敦司, 大和田 智彦, 藤本 博
    日本化学会第81回春季年会  2002/03
  • N6-シクロプロピルアデニンの一電子酸化反応  [Not invited]
    小川 敦司, 中谷 和彦, 齋藤 烈
    日本化学会第79春季年会  2001/03

Industrial Property Rights

Awards & Honors

  • 2010/04 日本化学会 日本化学会第90春季年会優秀講演賞(学術)
     
    受賞者: 小川 敦司

Research Grants & Projects

Social Contribution

  • 「細胞を創る」研究会 12.0 大会運営事務局・オーガナイザー
    Date (from-to) : 2019/10/17-2019/10/18
    Role : Organizing member
    Sponser, Organizer, Publisher  : 「細胞を創る」研究会
  • オープンキャンパス(実験)
    Date (from-to) : 2019/08/09
    Role : Lecturer
  • 2018年日本化学会中国四国支部大会実行委員
    Date (from-to) : 2018/11/18-2018/11/19
    Role : Organizing member
    Sponser, Organizer, Publisher  : 日本化学会中国四国支部
  • PROSセミナー世話人
    Date (from-to) : 2018/08/03
    Role : Presenter
  • オープンキャンパス(コース紹介)
    Date (from-to) : 2017/08/08-2017/08/09
    Role : Lecturer
  • 高大連携授業(研究紹介)
    Date (from-to) : 2017/08/04
    Role : Lecturer
  • 高大連携授業(研究紹介)
    Date (from-to) : 2017/06/22
    Role : Lecturer
  • 高大連携授業(研究紹介)
    Date (from-to) : 2017/05/17
    Role : Lecturer
  • 高大連携授業(研究紹介)
    Date (from-to) : 2016/08/25
    Role : Lecturer
  • オープンキャンパス(研究紹介)
    Date (from-to) : 2016/08/08-2016/08/09
    Role : Lecturer
  • サイエンス・リーダーズキャンプ(研究紹介)
    Date (from-to) : 2016/08/03
    Role : Lecturer
  • 高大連携授業(研究紹介)
    Date (from-to) : 2016/06/22
    Role : Lecturer
  • 新世代の生物有機化学研究会2016世話人
    Date (from-to) : 2016/06/11
    Role : Organizing member
  • PROSセミナー世話人
    Date (from-to) : 2016/06/10
    Role : Presenter
  • PROSセミナー世話人
    Date (from-to) : 2015/11/06
    Role : Presenter
  • サイエンス・リーダーズキャンプ(研究紹介)
    Date (from-to) : 2015/07/29
    Role : Lecturer
  • 高大連携授業(研究紹介)
    Date (from-to) : 2015/06/17
    Role : Lecturer
  • 第15回日本RNA学会年会世話人
    Date (from-to) : 2013/07/24-2013/07/26
    Role : Organizing member
    Sponser, Organizer, Publisher  : 日本RNA学会

Media Coverage

  • 研究室からこんにちは
    Date : 2011/03/26
    Publisher, broadcasting station: 南海放送
    Media report

愛媛大学教員活動実績

教育活動(B)

担当授業科目(B01)

  • 2019, the first semester, under graduate, 化学特別講義
  • 2019, the first semester, under graduate, 卒業研究Ⅰ
  • 2019, the first semester, under graduate, 生物化学基礎Ⅱ
  • 2019, the first semester, master course, 核酸化学特論
  • 2019, the first semester, master course, 核酸化学特論
  • 2019, the first semester, master course, 化学特別講義
  • 2019, the first semester, master course, 化学ゼミナールⅠ
  • 2019, the first semester, master course, 分子科学課題演習I
  • 2019, the first semester, under graduate, 生物化学基礎Ⅱ


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