研究者総覧

野見山 桂 (ノミヤマ ケイ)

  • 沿岸環境科学研究センター 准教授
Last Updated :2020/11/10

研究者情報

論文上での記載著者名

  • Kei Nomiyama

ホームページURL

ORCID ID

J-Global ID

プロフィール

  • 愛媛大学沿岸環境科学研究センター化学汚染・毒性解析部門 准教授


    野生動物に蓄積する様々な環境汚染物質の分析法開発とその曝露リスク評価法の開発に従事している。特に汚染物質の生体内代謝物やPPCPs等、比較的極性の高い化学物質を対象に体内動態の実態と影響について多層オミクス解析を用いた研究を進めている。

    また、百聞は一見に如かずをモットーに、ライフワークとして世界各地を訪れ環境に関わる野生動物や自然の写真を撮影しており、それらを用いた環境教育・自然保護普及活動にも取り組んでいる。


    現在の研究テーマ

    1.野生生物に残留するPOPs代謝物: 種特異的な蓄積パターンの解明とリスク評価

    2. PPCPsによる水圏生態系の汚染実態解明とリスク評価

    3.哺乳類に残留するイオン性化合物の脳移行と甲状腺ホルモンへの影響評価

    4.ペット動物の化学汚染:有機ハロゲン化合物および代謝物の暴露実態解明とリスク評価

    5.メタボロミクスによる環境汚染物質の生体影響評価

    6.POPs代謝物によるヒトへの汚染実態の解明

    7.海棲・陸棲哺乳類に対する有機汚染物質の残留機構と薬物代謝能の種差解明

    8.水環境中における有機汚染物質の挙動および酸化分解過程の解明

    9.極性環境汚染物質の新規分析法の開発


    研究室 HP: http://kanka.cmes.ehime-u.ac.jp/

    研究室 FB: https://www.facebook.com/kankaatcmes/

    共同利用・共同研究拠点 LaMer: http://lamer-cmes.jp/

    CMES: http://www.cmes.ehime-u.ac.jp/

    es-BANK: http://esbank-ehime.com/dnn/Default.aspx?alias=esbank-ehime.com/dnn/j


    FB: https://www.facebook.com/kei.nomiyama

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研究キーワード

  • PPCPs   スペシメンバンク   ペット動物   野生生物   環境汚染   ミトコンドリア   核内受容体   プロテオミクス   メタボロミクス   オミクス   トキシコロジー   wildlife   PCBs   PBDEs   異物代謝   環境分析化学   水環境化学   Environmental Analytical Chemistry   Aquatic Environmental Sciences   

研究分野

  • 環境・農学 / 環境負荷、リスク評価管理
  • 環境・農学 / 生物資源保全学
  • 環境・農学 / 環境影響評価 / 毒性学
  • 環境・農学 / 化学物質影響 / 微量化学物質
  • 環境・農学 / 放射線影響 / 微量化学物質

経歴

  • 2015年 - 現在  愛媛大学沿岸環境科学研究センター 化学汚染・毒性解析部門准教授
  • 2013年04月 - 現在  愛媛大学大学院理工学研究科(理学系)分子科学コース兼任
  • 2011年 - 2015年  愛媛大学沿岸環境科学研究センター 化学汚染・毒性解析部門講師
  • 2008年 - 2011年  愛媛大学沿岸環境科学研究センター 生態環境計測分野 (農学部環境保全コース兼任)助教
  • 2008年 - 2011年  Center for Marine Environmental Studies (CMES) Ehime University. Environmental chemistry and ecotoxicology
  • 2011年  - Center for Marine Environmental Studies (CMES)
  • 2007年04月 - 2008年03月  日本学術振興会熊本県立大学特別研究員(DC2)化学
  • 2005年04月 - 2007年03月  福岡大学資源循環・環境システム研究所リサーチ・アシスタント
  • Environmental chemistry and ecotoxicology

学歴

  •         - 2008年   熊本県立大学

所属学協会

  • 日本毒性学会   環境ホルモン学会 [正式名称;日本内分泌撹乱化学物質学会]   日本セトロジー研究会   SETAC   日本水環境学会   日本環境化学会   SETAC   

研究活動情報

論文

  • Kraisiri Khidkhan, Yoshinori Ikenaka, Takahiro Ichise, Shouta M M Nakayama, Hazuki Mizukawa, Kei Nomiyama, Hisato Iwata, Koji Arizono, Keisuke Takahashi, Keisuke Kato, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 108898 - 108898 2020年09月 [査読有り]
     研究論文(学術雑誌) 
    Neonicotinoid insecticides are used for agricultural and non-agricultural purposes worldwide. Pets are directly exposed to neonicotinoids in veterinary products and through environmental contamination. Cytochrome P450 (CYP) is among the most significant xenobiotic metabolizing enzymes that oxidizes several chemicals, including neonicotinoids. However, CYP activities and metabolite compositions of neonicotinoid metabolites are unknown in most domesticated pet species. Our objectives were to reveal the differences in metabolites of neonicotinoids (imidacloprid, clothianidin, and acetamiprid) and CYP activities among common pet species (cats and dogs), humans, and rats. The results indicated that the CYP-mediated neonicotinoid metabolism was different depending on species and each neonicotinoid. Among these four species, the kinetics of imidacloprid metabolism indicated that rats have the highest rate of oxidation of imidacloprid to 4OH-imidacloprid, while the greatest enzyme kinetics of imidacloprid metabolism to 5OH-imidacloprid were found in rats and humans. Clothianidin was rapidly metabolized to 1-methyl-3-nitroguanidine and dm-clothianidin in rats, but cats and humans showed the lowest formation of dm-clothianidin. CYP activities in metabolism of acetamiprid to dm-acetamiprid and N-acetyl-acetamiprid were determined to be significantly higher in humans compared to other species. However, further studies should be targeted at identifying the differences in hepatic metabolism of neonicotinoids in these species using recombinant CYP enzymes.
  • Kei Nomiyama, Yusuke Tsujisawa, Emiko Ashida, Syuji Yachimori, Akifumi Eguchi, Hisato Iwata, Shinsuke Tanabe
    Environmental science & technology 54 18 11386 - 11395 2020年09月 [査読有り]
     研究論文(学術雑誌) 
    Prenatal hydroxylated polychlorinated biphenyls (OH-PCBs) exposure may disrupt fetal brain development during the critical period of thyroid hormone (TH) action. However, there are limited studies on the OH-PCB transfer to the fetal brain, particularly in primates. In this study, we selected the Japanese macaque (Macaca fuscata) as a model animal for the fetal transfer of OH-PCBs in humans and revealed OH-PCB concentrations and their relationships in maternal and fetal blood, liver, and brain. l-thyroxine (T4)-like OH-PCBs including 4OH-CB187, a major congener in humans, were found in high proportions in the blood, liver, brain, and placenta of pregnant Japanese macaques. OH-PCBs were detected in the fetal brain and liver in the first trimester, indicating their transfer to the brain in the early pregnancy stage. 4OH-CB187 and 4OH-CB202 were the major congeners found in fetal brain, indicating that these T4-like OH-PCBs are transported from maternal blood to the fetal brain via the placenta. These results indicate that further studies are needed on the effects of OH-PCBs on the developing fetal brain.
  • Kraisiri Khidkhan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M M Nakayama, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    The Journal of veterinary medical science 82 7 978 - 982 2020年07月 [査読有り]
     研究論文(学術雑誌) 
    The knowledge of cytochrome P450 (CYP) expression involved in chemical exposure are necessary in clinical applications for the medication and prediction of adverse effects. The aim of this study was to evaluate the mRNA expression of CYP1-CYP3 families in cats exposed to BDE-209 for one year. All selected CYP isoforms showed no significant difference in mRNA expressions between control and exposure groups, however, CYP3A12 and CYP3A131 revealed tend to be two times higher in the exposure group compared to control group. The present results indicate that the chronic exposure of BDE209 could not alter CYP expression in the liver of cats. This result considered caused by the deficiency of CYP2B subfamily which is major metabolism enzyme of polybrominated diphenyl ethers (PBDEs) in cat.
  • Kraisiri Khidkhan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M M Nakayama, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Wageh Sobhy Darwish, Mitsuyoshi Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 226 108613 - 108613 2019年12月 [査読有り]
     研究論文(学術雑誌) 
    Cats have been known to be extremely sensitive to chemical exposures. To understand these model species' sensitivity to chemicals and their toxicities, the expression profiles of xenobiotic-metabolizing enzymes should be studied. Unfortunately, the characterization of cytochrome P450 (CYP), the dominant enzyme in phase I metabolism, in cats has not extensively been studied. Polychlorinated biphenyls (PCBs) are known as CYP inducers in animals, but the information regarding the PCB-induced CYP expression in cats is limited. Therefore, in the present study, we aimed to elucidate the mRNA expression of the CYP1-CYP3 families in the cat tissues and to investigate the CYP mRNA expression related to PCB exposure. In cats, the greatest abundance of CYP1-CYP3 (CYP1A2, CYP2A13, CYP2C41, CYP2D6, CYP2E1, CYP2E2, CYP2F2, CYP2F5, CYP2J2, CYP2U1, and CYP3A132) was expressed in the liver, but some extrahepatic isozymes were found in the kidney (CYP1A1), heart (CYP1B1), lung (CYP2B11 and CYP2S1) and small intestine (CYP3A131). In cats, CYP1A1, CYP1A2 and CYP1B1 were significantly upregulated in the liver as well as in several tissues exposed to PCBs, indicating that these CYPs were distinctly induced by PCBs. The strong correlations between 3,3',4,4'-tetrachlorobiphenyl (CB77) and CYP1A1 and CYP1B1 mRNA expressions were noted, demonstrating that CB77 could be a potent CYP1 inducer. In addition, these CYP isoforms could play an essential role in the PCBs biotransformation, particularly 3-4 Cl-PCBs, because a high hydroxylated metabolite level of 3-4 Cl-OH-PCBs was observed in the liver.
  • Keerthi S. Guruge, Prasun Goswami, Rumi Tanoue, Kei Nomiyama, R. G.S. Wijesekara, Tilak S. Dharmaratne
    Science of the Total Environment 690 683 - 695 2019年11月 [査読有り]
     
    © 2019 Pharmaceuticals and personal care products (PPCPs) are known as an emerging class of water contaminants due to their potential adverse effects on aquatic ecosystems. In this study, we conducted the first nationwide survey to understand the distribution and environmental risk of 72 PPCPs in surface waterways of Sri Lanka. Forty-one out of 72 targeted compounds were detected with total concentrations ranging between 5.49 and 993 ng/L in surface waterways in Sri Lanka. The highest level of PPCP contamination was detected in an ornamental fish farm. Sulfamethoxazole was found with the highest concentration (934 ng/L) followed by N,N-diethyl-meta-toluamide (202 ng/L) and clarithromycin (119 ng/L). Diclofenac, mefenamic acid, ibuprofen, trimethoprim, and erythromycin were detected ubiquitously throughout the country. Our data revealed that hospital and domestic wastewater, and aquaculture activities potentially contribute to the presence of PPCPs in Sri Lankan waterways. The calculated risk quotients indicated that several locations face medium to high ecological risk to aquatic organisms from ibuprofen, sulfamethoxazole, diclofenac, mefenamic acid, tramadol, clarithromycin, ciprofloxacin, triclocarban, and triclosan. The aforementioned compounds could affect aquatic organisms from different trophic levels like algae, crustacean and fish, and also influence the emergence of antibiotic resistant bacteria. These findings emphasize that a wide variety of pharmaceuticals have become pervasive environmental contaminants in the country. This data will serve to expand the inventory of global PPCP pollution. Further monitoring of PPCPs is needed in Sri Lanka in order to identify PPCP point sources and to implement strategies for contaminant reduction in wastewater to protect the aquatic ecosystem, wildlife, and human health.
  • Kohki Takaguchi, Hiroyuki Nishikawa, Hazuki Mizukawa, Rumi Tanoue, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shouta M M Nakayama, Yoshinori Ikenaka, Tatsuya Kunisue, Mayumi Ishizuka, Shinsuke Tanabe, Hisato Iwata, Kei Nomiyama
    The Science of the total environment 688 1172 - 1183 2019年10月 [査読有り]
     研究論文(学術雑誌) 
    Polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) might disrupt thyroid function. However, there is no clear evidence of PCB exposure disrupting thyroid hormone (TH) homeostasis in dogs and cats. The present study conducted in vivo experiments to evaluate the effects of a mixture of 12 PCB congeners (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187 and 202, each congener 0.5 mg/kg BW, i.p. administration) on serum TH levels in male dogs (Canis lupus familiaris) and male cats (Felis silvestris catus). In PCB-exposed dogs, the time courses of higher-chlorinated PCBs and L-thyroxine (T4)-like OH-PCBs (4-OH-CB107 and 4-OH-CB202) concentrations were unchanged or tended to increase, whereas those of lower-chlorinated PCBs and OH-PCBs tended to decrease after 24 h. In PCB-exposed cats, concentrations of PCBs increased until 6 h and then remained unchanged. The levels of lower-chlorinated OH-PCBs including 4'-OH-CB18 increased until 96 h and then decreased. In PCB-exposed dogs, free T4 concentrations were higher than those in the control group at 48 and 96 h after PCB administration and positively correlated with the levels of T4-like OH-PCBs, suggesting competitive binding of T4 and T4-like OH-PCBs to a TH transporter, transthyretin. Serum levels of total T4 and total 3,3',5-triiodo-L-thyronine (T3) in PCB-exposed dogs were lower than in the control group at 24 and 48 h and negatively correlated with PCB concentrations, implying that PCB exposure enhanced TH excretion by increasing TH uptake and TH conjugation enzyme activities in the dog liver. In contrast, no obvious changes in TH levels were observed in PCB-exposed cats. This could be explained by the lower levels of T4-like OH-PCBs and lower hepatic conjugation enzyme activities in cats compared with dogs. Different effects on serum TH levels in PCB-exposed dogs and cats are likely to be attributable to species-specific PCB and TH metabolism.
  • Kei Nomiyama, Akifumi Eguchi, Kohki Takaguchi, Jean Yoo, Hazuki Mizukawa, Tomoko Oshihoi, Shinsuke Tanabe, Hisato Iwata
    Toxicology and applied pharmacology 377 114620 - 114620 2019年08月 [査読有り]
     研究論文(学術雑誌) 
    Canis lupus familiaris (domestic dog) possess a high capacity to metabolize higher-chlorinated polychlorinated biphenyls (PCBs) to thyroid hormone (TH)-like hydroxylated PCB metabolites (OH-PCBs). As a result, the brain could be at high risk of toxicity caused by OH-PCBs. To evaluate the effect of OH-PCBs on dog brain, we analyzed OH-PCB levels in the brain and the metabolome of the frontal cortex following exposure to a mixture of PCBs (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187, and 202). 4-OH-CB202 and 4-OH-CB107 were major OH-PCBs in the brain of PCB-exposed dogs. These OH-PCBs were associated with metabolites involved in urea cycle, proline-related compounds, and purine, pyrimidine, glutathione, and amino-acid metabolism in dog brain. Moreover, adenosine triphosphate levels in the PCBs exposure group were significantly lower than in the control group. These results suggest that OH-PCB exposure is associated with a disruption in TH homeostasis, generation of reactive oxygen species, and/or disruption of oxidative phosphorylation (OXPHOS) in brain cells. Among them, OXPHOS disturbance could be associated with both disruptions in cellular amino-acid metabolism and urea cycle. Therefore, an OXPHOS activity assay was performed to evaluate the disruption of OXPHOS by OH-PCBs. The results indicated that 4-OH-CB107 inhibits the function of Complexes III, IV, and V of the electron transport chain, suggesting that 4-OH-CB107 inhibit these complexes in OXPHOS. The neurotoxic effects of PCB exposure may be mediated through mitochondrial toxicity of OH-PCBs in the brain.
  • Tanoue R, Margiotta-Casaluci L, Huerta B, Runnalls TJ, Eguchi A, Nomiyama K, Kunisue T, Tanabe S, Sumpter JP
    The Science of the total environment 664 915 - 926 2019年05月 [査読有り]
  • Eguchi A, Nomiyama K, Sakurai K, Kim Trang PT, Viet PH, Takahashi S, Iwata H, Tanabe S, Todaka E, Mori C
    Environmental pollution (Barking, Essex : 1987) 242 Pt A 98 - 105 2018年11月 [査読有り]
  • Ochiai M, Iida M, Agusa T, Takaguchi K, Fujii S, Nomiyama K, Iwata H
    Toxicological sciences : an official journal of the Society of Toxicology 165 1 118 - 130 2018年09月 [査読有り]
  • 薬物・環境化学物質代謝の種差と甲状腺機能
    野見山 桂
    日本甲状腺学会雑誌 42 - 47 2018年04月 [査読有り][招待有り]
     研究論文(学術雑誌)
  • Rumi Tanoue, Imari Kume, Yasuo Yamamoto, Kohki Takaguchi, Kei Nomiyama, Shinsuke Tanabe, Tatsuya Kunisue
    Journal of Chromatography A 1539 30 - 40 2018年03月 [査読有り]
     研究論文(学術雑誌) 
    Thyroid hormones (THs), which mainly consist of 3, 3′ 5-triiodo-L-thyronine (T3) and L-thyroxine (T4), play a critical role in regulating biological processes such as growth and metabolism in various animal species. Thus, accurate measurement of T3 and T4, especially physiologically active free (protein-unbound) forms, in serum/plasma is needed for the evaluation of TH homeostasis. However, such high-precision determination of free THs is lacking for non-human species. The present study aimed to develop a highly sensitive and reliable liquid chromatography-tandem mass spectrometry (LC–MS/MS) method for the determination of six free THs in serum/plasma, which is applicable to not only humans but also non-human species. Two different physical separation steps, ultrafiltration (UF) and equilibrium dialysis (ED), were examined to obtain the free TH fraction. Several experimental conditions were carefully optimized and validated for UF or ED using the commercially available bovine serum. As a result, UF at 1100 × g and 37 °C for 30 min with a 30 kDa ultrafiltration device (Centrifree YM-30, Millipore) yielded excellent precision (CV: < 10%). The optimized ED step also yielded high precision (CV: < 10%) and the measurement values were approximately equal to those of UF, but at least 16 h were required to reach equilibrium. Thus, UF combined with LC–MS/MS was finally chosen, in terms of the time needed for the measurement. Acceptable accuracy (recovery: 70%–110%) and intra- and inter-day precision (CV: < 10% and < 12%, respectively) were obtained, when triplicate analyses in three different days were conducted using the bovine serum. The developed analytical method was successfully applied to the determination of free THs in serum/plasma samples of humans, cats, and dogs. Furthermore, comparison with free T4 concentrations measured by a common immunoassay method evidently indicated that the ultrafiltration-LC–MS/MS method developed in this study can increase the specificity and accuracy of TH measurement.
  • ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響
    水川 葉月, 高居 名菜, 野見山 桂, 高口 倖暉, 横山 望, 市居 修, 滝口 満喜, 池中 良徳, 中山 翔太, 石塚 真由美
    環境ホルモン学会研究発表会要旨集 20回 78 - 78 環境ホルモン学会(日本内分泌撹乱化学物質学会) 2017年12月 [査読有り]
  • Chihiro Ishii, Shouta M. M. Nakayama, Yoshinori Ikenaka, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Hazuki Mizukawa, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Mayumi Ishizuka
    CHEMOSPHERE 186 367 - 373 2017年11月 [査読有り]
     研究論文(学術雑誌) 
    Lead (Pb) poisoning is widespread among raptors and water birds. In Japan, fragments of Pb ammunition are still found in endangered eagles although more than 10 years have passed since legislation regarding use of Pb ammunition was introduced. This study was performed to investigate Pb exposure in raptors from various locations in Japan. We measured hepatic and renal Pb concentrations and hepatic Pb isotope ratios of Steller's sea eagles (Haliaeetus pelagicus), white-tailed sea eagles (Haliaeetus albicilla), golden eagles (Aquila chrysaetos), and 13 other species (total 177 individuals) that were found dead, as well as blood samples from three eagles found in a weakened state during 1993-2015 from Hokkaido (northern part), Honshu (the main island), and Shikoku (a southern island) of japan. In the present study in Hokkaido, one quarter of the sea eagles showed a high Pb concentration, suggesting exposure to abnormally high Pb levels and Pb poisoning. Pb isotope ratios indicated that endangered Steller's sea eagle and white-tailed sea eagle were poisoned by Pb ammunition that was used illegally in Hokkaido. In other areas of Japan, both surveillance and regulations were less extensive than in Hokkaido, but Pb poisoning in raptors was also noted. Therefore, Pb poisoning is still a serious problem in raptors in various areas of Japan due to accidental ingestion of materials containing Pb, especially Pb ammunition. (C) 2017 Published by Elsevier Ltd.
  • Rumi Tanoue, Luigi Margiotta-Casaluci, Belinda Huerta, Tamsin J. Runnalls, Kei Nomiyama, Tatsuya Kunisue, Shinsuke Tanabe, John P. Sumpter
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 51 21 12825 - 12835 2017年11月 [査読有り]
     研究論文(学術雑誌) 
    Recent species-extrapolation approaches to the prediction of the potential effects of pharmaceuticals present in the environment on wild fish are based on the assumption that pharmacokinetics and metabolism in humans and fish are comparable. To test this hypothesis, we exposed fathead minnows to the opiate pro-drug tramadol and examined uptake from the water into the blood and brain and the metabolism of the drug into its main metabolites. We found that plasma concentrations could be predicted reasonably accurately based on the lipophilicity of the drug once the pH of the water was taken into account. The concentrations of the drug and its main metabolites were higher in the brain than in the plasma, and the observed brain and plasma concentration ratios were within the range of values reported in mammalian species. This fish species was able to metabolize the pro-drug tramadol into the highly active metabolite O-desmethyl tramadol and the inactive metabolite N-desmethyl tramadol in a similar manner to that of mammals. However, we found that concentration ratios of O-desmethyl tramadol to tramadol were lower in the fish than values in most humans administered the drug. Our pharmacokinetic data of tramadol in fish help bridge the gap between widely available mammalian pharmacological data and potential effects on aquatic organisms and highlight the importance of understanding drug uptake and metabolism in fish to enable the full implementation of predictive toxicology approaches.
  • Nguyen Minh Tue, Akitoshi Goto, Shin Takahashi, Takaaki Itai, Kwadwo Ansong Asante, Kei Nomiyama, Shinsuke Tanabe, Tatsuya Kunisue
    JOURNAL OF MATERIAL CYCLES AND WASTE MANAGEMENT 19 4 1324 - 1332 2017年10月 [査読有り]
     研究論文(学術雑誌) 
    Detailed composition of chlorinated and brominated polycyclic aromatic hydrocarbons (Cl-PAHs and Br-PAHs) generated during informal recycling of e-waste and their toxic relevance are still poorly understood. This study investigated the occurrence of Cl-PAHs and Br-PAHs in surface soil samples from the Agbogbloshie e-waste recycling site (Accra, Ghana) using quantitative gas chromatography-mass spectrometry (GC-MS) and comprehensive two-dimensional GC-time-of-flight mass spectrometry (GC x GC-ToFMS) profiling. The results of GC-MS analysis showed elevated concentrations in open e-waste burning areas (160-220 and 19-46 ng/g dry weight for Cl- and Br-PAHs, respectively) with substantial contribution from unidentified compounds (respectively, more than 36 and 70%, based on the total areas of potential peaks). Cl- and Br-PAHs from e-waste burning had a distinctive composition dominated by ring-ring compounds. Several homologue groups not monitored with GC-MS were found using GC x GC-ToFMS: PAHs with up to 5 Cl or 3Br, mixed halogenated PAHs and chlorinated methylPAHs. The dioxin-like toxic equivalents of the identified Cl-/Br-PAHs in soils, estimated from their in vitro AhR agonist potencies relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin, were much lower than the range reported for chlorinated and brominated dioxins. However, the toxicity of the unidentified halogenated PAHs remained unclear.
  • Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Miyuki Yamamoto, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 51 19 11354 - 11362 2017年10月 [査読有り]
     研究論文(学術雑誌) 
    Present study determined concentrations and residue patterns of bromophenols (BPhs) in whole blood samples of pet cats and pet dogs collected from veterinary hospitals in Japan. BPhs concentrations were higher in cat blood than in dog blood, with statistically insignificant differences (p = 0.07). Among the congeners, 2,4,6-tribromophenol (TBPh) constituted the majority of BPhs (>90%) detected in both species. Analysis of commercial pet food to estimate exposure routes showed that the most abundant congener in all pet food samples was 2,4,6-TBPh, accounting for >99% of total BPhs. This profile is quite similar to the blood samples of the pets, suggesting that diet might be an important exposure route for BPhs in pets. After incubation in polybrominated diphenyl ether (PBDE) mixtures (BDE-47, BDE-99 and BDE-209), 2,4,5-TBPh was found in dog liver microsomes but not in cat liver microsomes, implying species-specific metabolic capacities for PBDEs. Formation of 2,4,5-TBPh occurred by hydroxylation at the 1' carbon atom of the ether bond of BDE-99 is similar to human study reported previously. Hydroxylated PBDEs were not detected in cats or dogs; therefore, diphenyl ether bond cleavage of PBDEs can also be an important metabolic pathway for BPhs formation in cats and dogs.
  • Mari Ochiai, Kei Nomiyama, Tomohiko Isobe, Tadasu K. Yamada, Yuko Tajima, Ayaka Matsuda, Akira Shiozaki, Takashi Matsuishi, Masao Amano, Hisato Iwata, Shinsuke Tanabe
    MARINE ENVIRONMENTAL RESEARCH 128 124 - 132 2017年07月 [査読有り]
     研究論文(学術雑誌) 
    This study investigated the accumulation of polybrominated diphenyl ethers (PBDEs) and their hydroxylated and methoxylated analogues (OH-PBDEs and MeO-PBDEs) in the blood of harbor porpoises, Dall's porpoises, and finless porpoises stranded or bycaught in Japanese coastal waters and in the North Pacific Ocean. Moreover, we suggested the origins of these contaminants and the factors affecting their pattern of accumulation. Levels of PBDEs in Dall's porpoises were one order of magnitude greater than those in the other species. OH-PBDE and MeO-PBDE levels were comparable to those of PBDEs. However, no correlation was found between the levels of OH-PBDEs and PBDEs, whereas a strong correlation was found between that of OH-PBDEs and MeO-PBDEs (p < 0.001). 6OH-BDE47, reported compound bio-synthesized by marine low-trophic level organisms, was the dominant congener. These results suggest that PBDEs found in these porpoise species derive from flame retardants, but OH-PBDEs and MeO-PBDEs are mainly of natural origins. (C) 2016 Elsevier Ltd. All rights reserved.
  • Takamitsu Kondo, Yoshinori Ikenaka, Shouta M. M. Nakayama, Yusuke K. Kawai, Hazuki Mizukawa, Yoko Mitani, Kei Nomiyama, Shinsuke Tanabe, Mayumi Ishizuka
    TOXICOLOGICAL SCIENCES 158 1 90 - 100 2017年07月 [査読有り]
     研究論文(学術雑誌) 
    UDP-glucuronosyltransferases (UGTs) are among the most important xenobiotic metabolizing enzymes that conjugate a wide range of chemicals. Previous studies showed that Felidae and Pinnipedia species have very low UGT activities toward some phenolic compounds because of the UGT1A6 pseudogene and small numbers of UGT1A isozymes. In addition to the UGT1As, UGT2Bs isozymes also conjugate various endogenous (eg, estrogens, androgens, and bile acids) and exogenous compounds (opioids, non-steroidal anti-inflammatory drugs, and environmental pollutants). However UGT2B activity and genetic background are unknown in carnivore species. Therefore, this study was performed to elucidate the species differences of UGT2Bs. Using typical substrates for UGT2Bs, UGT activity was measured in vitro. In addition, UGT2B genetic features are analyzed in silico. Results of UGT activity measurement indicate marked species differences between dogs and other carnivores (cats, Northern fur seals, Steller sea lions, Harbor seals, and Caspian seals). Dogs have very high V-max/K-m toward estradiol (17-glucuronide), estrone, lorazepam, oxazepam, and temazepam. Conversely, cats and pinniped species (especially Caspian seals and Harbor seals) have very low activities toward these substrates. The results of genetic synteny analysis indicate that Felidae and pinniped species have very small numbers of UGT2B isozymes (one or none) compared with dogs, rodents, and humans. Furthermore, Felidae species have the same nonsense mutation in UGT2B, which suggests that Felidae UGT2B31-like is also a pseudogene in addition to UGT1A6. These findings of lower activity of UGT2B suggest that Felidae and some pinniped species have very low UGT activity toward a wide range of chemicals. These results are important for Felidae and Pinnipedia species that are frequently exposed to drugs and environmental pollutants.
  • ネコの甲状腺機能に対するポリ塩化ビフェニル(PCBs)の影響評価 甲状腺ホルモンの脱ヨード化と代謝能の解析
    水川 葉月, 高居 名菜, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 高口 倖暉, 西川 博之, 池中 良徳, 中山 翔太, Sainnoxoi Tsend-ayush, 田辺 信介, 石塚 真由美
    The Journal of Toxicological Sciences 42 Suppl. S236 - S236 (一社)日本毒性学会 2017年06月 [査読有り]
  • Kei Nomiyama, Kohki Takaguchi, Hazuki Mizukawa, Yasuko Nagano, Tomoko Oshihoi, Susumu Nakatsu, Tatsuya Kunisue, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 51 10 5811 - 5819 2017年05月 [査読有り]
     研究論文(学術雑誌) 
    The adverse effects of elevated polybrominated diphenyl ether (PBDE) levels, reported in the blood of domestic dogs and cats, are considered to be of great concern. However, the tissue distribution of PBDEs and their derivatives in these animals is poorly understood. This study determined the concentrations and profiles of PBDEs, hydroxylated PBDEs (OH-PBDEs), methoxylated PBDEs (MeO-PBDEs), and 2,4,6-tribromophenol (2,4,6-tri-BPh) in the blood, livers, bile, and brains of dogs and cats in Japan. Higher tissue concentrations of PBDEs were found in cats, with the dominant congener being BDE209. BDE207 was also predominant in cat tissues, indicating that BDE207 was formed via BDE209 debromination. BDE47 was the dominant congener in dog bile, implying a species-specific excretory capacity of the liver. OH-PBDE and MeO-PBDE concentrations were several orders of magnitude higher in cat tissues, with the dominant congener being 6OH-BDE47, possibly owing to their intake of naturally occurring MeO-PBDEs in food, MeO-PBDE demethylation in the liver, and lack of UDP-glucuronosyltransferase, UGT1A6. Relatively high concentrations of BDE209, BDE207, 6OH-BDE47, 2'MeO-BDE68, and 2,4,6-tri-BPh were found in cat brains, suggesting a passage through the blood brain barrier. Thus, cats in Japan might be at a high risk from PBDEs and their derivatives, particularly BDE209 and 6OH-BDE47.
  • Kenshi Sankoda, Izumi Toda, Kazuhiko Sekiguchi, Kei Nomiyama, Ryota Shinohara
    CHEMOSPHERE 171 399 - 404 2017年03月 [査読有り]
     研究論文(学術雑誌) 
    We examined the secondary production of halogenated derivatives of polycyclic aromatic hydrocarbons (PAHs) in surface seawater. Pyrene was selected as the model compound and exposed to UV irradiation in synthetic seawater for various irradiation times. Pyrene underwent rapid photochemical reactions that produced various halogenated derivatives including 1-chloropyrene, 1-bromopyrene, three unidentified dichloropyrenes, and three unidentified bromochloropyrenes. The production of 1-chloropyrene (220-360 nM) was higher than that of 1-bromopyrene (7.3-12 nM), reflecting the high chlorine content of seawater. A pilot field survey was conducted to test the environmental implications of these results, and fresh, brackish, and seawater samples were collected in southwestern Japan. The variation in the concentration ratios between 1-chloropyrene and pyrene implied the presence of a specific source of 1-chloropyrene in coastal water, which can be partly explained by the secondary production observed in our photolysis experiments. In sum, the photochemical reactions of PAHs are a potential secondary source of halogenated PAHs, especially in marine environments heavily contaminated with PAHs. (C) 2016 Elsevier Ltd. All rights reserved.
  • Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Hisato Iwata, Jean Yoo, Akira Kubota, Miyuki Yamamoto, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Tatsuya Kunisue, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 50 1 444 - 452 2016年01月 [査読有り]
     研究論文(学術雑誌) 
    There are growing concerns about the increase in hyperthyroidism in pet cats due to exposure to organo-halogen contaminants and their hydroxylated metabolites. This study investigated the blood contaminants polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) and their hydroxylated and methoxylated derivatives (OH-PCBs, OH-PBDEs, and MeO-PBDEs), in pet dogs and cats. We also measured the residue levels of these compounds in commercially available pet foods. Chemical analyses of PCBs and OH-PCBs showed that the OH-PCB levels were 1 to 2 orders of magnitude lower in cat and dog food products than in their blood, suggesting that the origin of OH-PCBs in pet dogs and cats is PCBs ingested with their food. The major congeners of OH-/MeO-PBDEs identified in both pet food products and blood were natural products (60H-/MeO-BDE47 and 2'OH-/MeO-BDE68) from marine organisms. In particular, higher concentrations of 60H-BDE47 than 2'OH-BDE68 and two MeO-PBDE congeners were observed in the cat blood, although MeO-BDEs were dominant in cat foods, suggesting the efficient biotransformation of 60H-BDE47 from 6MeO-BDE47 in cats. We performed in vitro demethylation experiments to confirm the biotransformation of MeO-PBDEs to OH-PBDEs using liver microsomes. The results showed that 6MeO-BDE47 and 2'MeO-BDE68 were demethylated to 60H-BDE47 and 2'OH-BDE68 in both animals, whereas no hydroxylated metabolite from BDE47 was detected. The present study suggests that pet cats are exposed to MeO-PBDEs through cat food products containing fish flavors and that the OH-PBDEs in cat blood are derived from the CYP-dependent demethylation of naturally occurring MeO-PBDE congeners, not from the hydroxylation of PBDEs.
  • Jean Yoo, Masashi Hirano, Hazuki Mizukawa, Kei Nomiyama, Tetsuro Agusa, Eun-Young Kim, Shinsuke Tanabe, Hisato Iwata
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 49 24 14588 - 14596 2015年12月 [査読有り]
     研究論文(学術雑誌) 
    The aim of this study was to understand the cytochrome P450 (CYP)-dependent metabolic pathway and potency of polychlorinated biphenyls (PCBs) in the Baikal seal (Pusa sibirica). In vitro metabolism of 62 PCB congener mixtures was investigated by using liver microsomes of this species. A decreased ratio of over 20% was observed for CB3, CB4, CB8, CB15, CB19, CB22, CB37, CB54, CB77, and CB105, suggesting the preferential metabolism of low-chlorinated PCBs by CYPs. The highly activated metabolic pathways in Baikal seals that were predicted from the decreased PCBs and detected hydroxylated PCBs (OH-PCBs) were CB22 to 4'OH-CB20 and CB77 to 4'OH-CB79. The total amount of OH-PCBs detected as identified and unidentified congeners accounted for only a 3.8 +/- 1.7 mol % of loaded PCBs, indicating many unknown PCB metabolic pathways. To explore factors involved in CYP-dependent PCB metabolism, we examined the relationships among the structural and physicochemical properties of PCBs, the in silica PCB-CYP docking parameters, and the in vitro PCB decreased ratios by principal component analysis. Statistical analysis showed that the decreased PCB ratio was at least partly accounted for by the substituted chlorine number of PCBs and the distance from the CI-unsubstituted carbon of docked PCBs to the heme Fe in CYP2A and 2B.
  • Mayu Kakehi, Yoshinori Ikenaka, Shouta M. M. Nakayama, Yusuke K. Kawai, Kensuke P. Watanabe, Hazuki Mizukawa, Kei Nomiyama, Shinsuke Tanabe, Mayumi Ishizuka
    TOXICOLOGICAL SCIENCES 147 2 360 - 369 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    There are various interspecies differences in xenobiotic-metabolizing enzymes. It is known that cats show slow glucuronidation of drugs such as acetaminophen and strong side effects due to the UGT1A6 pseudogene. Recently, the UGT1A6 pseudogene was found in the Northern elephant seal and Otariidae was suggested to be UGT1A6-deficient. From the results of measurements of uridine diphosphate-glucuronosyltransferase (UGT) activity using liver microsomes, the Steller sea lion, Northern fur seal, and Caspian seal showed UGT activity toward 1-hydroxypyrene and acetaminophen as low as in cats, which was significantly lower than in rat and dog. Furthermore, UGT1A6 pseudogenes were found in Steller sea lion and Northern fur seal, and all Otariidae species were suggested to have the UGT1A6 pseudogene. The UGT1 family genes appear to have undergone birth-and-death evolution based on a phylogenetic and synteny analysis of the UGT1 family in mammals including Carnivora. UGT1A2-1A5 and UGT1A7-1A10 are paralogous genes to UGT1A1 and UGTA6, respectively, and their numbers were lower in cat, ferret and Pacific walrus than in human, rat, and dog. Felidae and Pinnipedia, which are less exposed to natural xenobiotics such as plant-derived toxins due to their carnivorous diet, have experienced fewer gene duplications of xenobiotic-metabolizing UGT genes, and even possess UGT1A6 pseudogenes. Artificial environmental pollutants and drugs conjugated by UGT are increasing dramatically, and their elimination to the environment can be of great consequence to cat and Pinnipedia species, whose low xenobiotic glucuronidation capacity makes them highly sensitive to these compounds.
  • Rumi Tanoue, Kei Nomiyama, Haruna Nakamura, Joon-Woo Kim, Tomohiko Isobe, Ryota Shinohara, Tatsuya Kunisue, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 49 19 11649 - 11658 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    A fish plasma model (FPM) has been proposed as a screening technique to prioritize potential hazardous pharmaceuticals to wild fish. However, this approach does not account for inter- or intraspecies variability of pharmacokinetic and pharmacodynamic parameters. The present study elucidated the uptake potency (from ambient water), tissue distribution, and biological risk of 20 pharmaceutical and personal care product (PPCP) residues in wild cyprinoid fish inhabiting treated-wastewater-impacted streams. In order to clarify the uncertainty of the FPM for PPCPs, we compared the plasma bioaccumulation factor in the field (BAF(plasma) = measured fish plasma/ambient water concentration ratio) with the predicted plasma bioconcentration factor (BCFplasma = fish plasma predicted by use of theoretical partition coefficients/ambient water concentration ratio) in the actual environment. As a result, the measured maximum BAF(plasma) of inflammatory agents was up to 17 times higher than theoretical BCFplasma values, leading to possible underestimation of toxicological risk on wild fish. When the tissue blood partition coefficients (tissue/blood concentration ratios) of PPCPs were estimated, higher transportability into tissues, especially the brain, was found for psychotropic agents, but brain/plasma ratios widely varied among individual fish (up to 28-fold). In the present study, we provide a valuable data set on the intraspecies variability of PPCP pharmacokinetics, and our results emphasize the importance of determining PPCP concentrations in possible target organs as well as in the blood to assess the risk of PPCPs on wild fish.
  • Hazuki Mizukawa, Kei Nomiyama, Tatsuya Kunisue, Michio X. Watanabe, Annamalai Subramanian, Hisato Iwata, Mayumi Ishizuka, Shinsuke Tanabe
    ENVIRONMENTAL RESEARCH 138 255 - 263 2015年04月 [査読有り]
     研究論文(学術雑誌) 
    The concentrations of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and their hydroxylated metabolites (OH-PCBs and OH-PBDEs) were measured in the blood of Eurasian wild pigs (Sus scrofa) from a municipal waste open dumping site (DS) and a reference site (RS) in South India. We showed that contamination with OH-PCBs was higher in female pigs from the DS than in all other adult pigs. The highest OH-PCB concentrations were found in piglets from the DS. Moreover, the hepatic expression levels of CYP1A and CYP2B were higher in piglets than in their dam, implying metabolism of PCBs by cytochrome P450 (CYP) enzymes. The OH-PCB congener profiles differed according to sex and collection sites, possibly because of variations in the expression levels of phase I and phase II enzymes among individual pigs, differences in the exposure sources, and maternal transfer of parent PCBs. The hepatic CYP1A expression levels were positively correlated with the blood concentrations of 4OH-CB107, 4OH-CB162, and 4OH-CB187, implying CYP1A-dependent formation of these OH-PCBs in the pig liver. We found no significant correlations between the blood concentrations of OH-PCBs and thyroid hormones (THs); however, the thyroxin (T-4) levels were lower in pigs from the DS than in pigs from the RS. Our limited dataset suggest that induced CYP enzymes accelerate the metabolism of xenobiotics and endogenous molecules in pigs. Thus, besides parental compounds, the risk of hydroxylated metabolites entering wildlife and humans living in and around municipal open waste dumping sites should be considered. (C) 2015 Elsevier Inc. All rights reserved.
  • Akifumi Eguchi, Kei Nomiyama, Nguyen Minh Tue, Pham Thi Kim Trang, Pham Hung Viet, Shin Takahashi, Shinsuke Tanabe
    ENVIRONMENTAL RESEARCH 137 440 - 449 2015年02月 [査読有り]
     研究論文(学術雑誌) 
    This study demonstrated the contamination levels of polychlorinated biphenyls (PCBs), hydroxylated PCBs (OH-PCBs), polybrominated diphenyl ethers (PBDEs), methoxylated PBDEs (MeO-PBDEs), hydroxylated PBDEs (OH-PBDEs), and bromophenols (BPhs), and their relationships with thyroid hormones (THs), in the serum of human donors from an e-waste recycling site and a rural site in Hung Yen province, Vietnam. Occupationally related exposure was indicated by significantly higher residue levels of PCBs, OH-PCBs, PBDEs, and BPhs in the serum of donors from the e-waste recycling site (median: 420, 160, 290, and 300 pg g(-1) wet wt, respectively) than those in the serum of donors from the rural site (median: 290, 82, 230, and 200 pg g(-1) wet wt, respectively). On the other hand, levels of OH-/MeO-PBDEs were significantly higher in serum of donors from the reference site (median: 160 and 20 pg g(-1) wet wt, respectively) than in those from the e-waste recycling site (median: 43 and 0.52 pg g(-1) wet wt, respectively). In addition, we implemented stepwise generalized linear models to assess the association between the levels of TH and PCBs, PBDEs, and their related compounds. In females, we found positive associations of PCBs and OH-PCB concentrations with total thyroxine, free thyroxine, total triiodothyronine, and free triiodothyronine, and a negative association with thyroid-stimulating hormone concentrations. (C) 2015 Elsevier Inc. All rights reserved.
  • Kenshi Sankoda, Kotaro Murata, Mai Tanihata, Kengo Suzuki, Kei Nomiyama, Ryota Shinohara
    ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 68 2 217 - 224 2015年02月 [査読有り]
     研究論文(学術雑誌) 
    This study aimed to investigate the behavior of organic ultraviolet (UV) filters released by recreational activities along the Japanese coastline. Seasonal variations of organic UV filters in seawater were investigated at four different recreational beaches (Mogushi, Wakamiya, Tsurugahama, and Otachimisaki beaches) in both summer (July through August) and winter (December). Moreover, short time scale diurnal changes were monitored at Otachimisaki beach in summer. Of the four sunscreen agents tested in this study, two agents-2-ethylhexyl-4-methoxycinnamate (EHMC) and 2-ethylhexyl salicylate (EHS)-were detected in all samples, whereas octyl-dimethyl-p-aminobenzonic acid and 3-(4-methylbenzylidene)-camphor were lower than detection limits. In particular, EHMC, one of the most popular organic UV filters, was dominant. The highest concentration of EHMC was observed at 1,080 ng L-1, a level that exceeds those of previous studies. Both EHMC and EHS concentrations showed significant (p < 0.05) seasonal variations with advancing summer suggesting direct input from recreational activities. The subsequent examination showed short time scale diurnal changes of organic UV filters on the beach. The results showed that diurnal changes in EHMC concentrations were correlated to the number of bathers. EHMC concentrations increased during the afternoon and decreased during the night, although complete attenuation during the night did not occur. EHMC persists along the coast due to low mobility and may persist the next day. This is the first study to show the natural attenuation behavior of organic UV filters along recreational beaches.
  • Daisuke Imaeda, Kei Nomiyama, Tatsuya Kunisue, Hisato Iwata, Oyuna Tsydenova, Masao Amano, Evgeny A. Petrov, Valeriy B. Batoev, Shinsuke Tanabe
    CHEMOSPHERE 114 1 - 8 2014年11月 [査読有り]
     研究論文(学術雑誌) 
    We have previously demonstrated that Baikal seals (Pusa sibirica) are still being exposed to polychlorinated biphenyls (PCBs), and the population is at risk. In the present study, we measured the residue levels of PCBs and their hydroxylated metabolites (OH-PCBs) in the blood of Baikal seals and assessed the impact of OH-PCBs on the thyroid function. Blood concentrations of PCBs and OH-PCBs were in the range of 2.8130 ng g(-1) wet wt. and 0.71-4.6 ng g(-1) wet wt., respectively. Concentrations of higher-chlorinated OH-PCBs (hexa- to octa-PCBs) were more than 70% to total OH-PCB concentrations, indicating Baikal seals are mostly risked by higher-chlorinated OH-PCBs. High levels of 4OH-CB146 and 4OH-CB187 and low levels of 4OH-CB107/4'OH-CB108 found in Baikal seals were different from those in other phocidae species, suggesting the unique drug-metabolizing enzyme activities and/or contamination sources in this species. Concentrations of some OH-PCBs in males were significantly higher than those in females. These results suggest that these isomers may be preferentially transferred from mother to pup via cord blood. However, concentrations of almost all the isomers were not significantly correlated with the levels of blood total T3 and T4, implying less impact of PCB-related compounds on the thyroid hormone circulation. (C) 2014 Elsevier Ltd. All rights reserved.
  • Kei Nomiyama, Shusaku Hirakawa, Akifumi Eguch, Chika Kanbara, Daisuke Imaeda, Jean Yoo, Tatsuya Kunisue, Eun-Young Kim, Hisato Iwata, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 48 22 13530 - 13539 2014年11月 [査読有り]
     研究論文(学術雑誌) 
    We have previously reported that high accumulation of dioxins and related compounds induced cytochrome P450 (CYP 1s) isozymes in the liver of wild Baikal seals, implying the enhanced hydroxylation of polychlorinated biphenyls (PCBs). The present study attempted to elucidate the residue concentrations and patterns of PCBs and hydroxylated PCBs (OH-PCBs) in the livers of Baikal seals. The hepatic residue concentrations were used to assess the potential effects of PCBs and OH-PCBs in combination with the analyses of serum thyroid hormones, hepatic mRNA levels, and biochemical markers. The hepatic expression levels of CYP1 genes were positively correlated with the concentration of each OH-PCB congener. This suggests chronic induction of these CYP1 isozymes by exposure to PCBs and hydroxylation of PCBs induced by CYP 1s. Hepatic mRNA expression monitoring using a custom microarray showed that chronic exposure to PCBs and their metabolites alters the gene expression levels related to oxidative stress, iron ion homeostasis, and inflammatory responses. In addition, the concentrations of OH-PCBs were negatively correlated with l-thyroxine (T-4) levels and the ratios of 3,3',5-triiodo-l-thyronine (T-3)/reverse 3,3',5'-triiodo-l-thyroninee (rT(3)). These observations imply that Baikal seals contaminated with high levels of OH-PCBs may undergo the disruption of mechanisms related to the formation (or metabolism) of T-3 and T-4 in the liver.
  • Rumi Tanoue, Kei Nomiyama, Haruna Nakamura, Terutake Hayashi, Joon-Woo Kim, Tomohiko Isobe, Ryota Shinohara, Shinsuke Tanabe
    JOURNAL OF CHROMATOGRAPHY A 1355 193 - 205 2014年08月 [査読有り]
     研究論文(学術雑誌) 
    In the present study, a sensitive and accurate isotope dilution method was developed for the simultaneous determination of 17 polar pharmaceutical and personal care product (PPCP) residues (logK(ow) = 1.40-5.74), including 14 pharmaceuticals and 3 personal care products, in biological organs and tissues. The proposed method involved enzymatic hydrolysis, followed by sequential clean-up using silica gel chromatography and gel permeation chromatography, and analysis via ultra-high-performance liquid chromatography with tandem mass spectrometry. This method yielded acceptable absolute recoveries (48-88%) and internal standard-corrected recoveries (90-130%) for 17 PPCPs. Method detection limits were between 0.0092 and 3.2 ng g(-1) wet weight, and the limits of quantification were between 0.020 and 8.7 ng g(-1) wet weight. The method can be used to readily detect the target compounds at trace levels while minimizing the required sample volume. The developed method was applied to the determination of 17 PPCPs in the liver and kidney of 17 birds collected from Japan and also in the plasma, liver, and brain of 7 cyprinoid fish from an effluent-dominated stream in Japan. Triclosan was detected in 5 of 11 fish-eating birds but not in non-fish-eating birds, suggesting the contamination of prey fish by the chemical. Non-steroidal anti-inflammatory drugs, antibacterial agents, and psychotropic agents were frequently detected in the fish tissues. In addition, 7 of the target compounds were found in fish brain. The median brain/plasma ratios of the psychotropic agents ranged from 1.6 (carbamazepine) to 12 (diphenhydramine), indicating high transportability to fish brain. (C) 2014 Elsevier B.V. All rights reserved.
  • Kei Nomiyama, Chika Kanbara, Mari Ochiai, Akifumi Eguchi, Hazuki Mizukawa, Tomohiko Isobe, Takashi Matsuishi, Tadasu K. Yamada, Shinsuke Tanabe
    MARINE ENVIRONMENTAL RESEARCH 93 15 - 22 2014年02月 [査読有り]
     研究論文(学術雑誌) 
    Information on accumulation of halogenated phenolic contaminants in the blood of marine mammal is limited. The present study, we determined the residue levels and patterns of chlorinated and brominated phenolic contaminants (OH-PCBs, OH-PBDEs and bromophenols) in the blood collected from pinnipeds (northern fur seal, spotted seal, Steller sea lion and ribbon seal) and small cetaceans (harbor porpoise and Dall's porpoise) from Japanese coastal waters. Concentrations of PCBs and OH-PCBs found in pinnipeds were the same as in small cetaceans living in the same coastal area. However, significantly lower concentrations of brominated compounds (PBDEs, MeO-PBDEs, OH-PBDEs) were found in the blood of pinnipeds than the levels found in cetacean species which live same area (p < 0.05). This difference of accumulation pattern suggested pinnipeds have an enhanced capability to degrade organobromine compounds relative to cetaceans. (C) 2013 Elsevier Ltd. All rights reserved.
  • Akifumi Eguchi, Kei Nomiyama, Mari Ochiai, Hazuki Mizukawa, Yasuko Nagano, Katsuhiro Nakagawa, Kouki Tanaka, Haruhiko Miyagawa, Shinsuke Tanabe
    TALANTA 118 253 - 261 2014年01月 [査読有り]
     研究論文(学術雑誌) 
    In this study, we developed a comprehensive, highly sensitive, and robust method for determining 53 congeners of three to eight chlorinated OH-PCBs in liver and brain samples by using isotope dilution gas chromatography (GC) coupled with electron capture negative ionization mass spectrometry (ECNI-MS). These results were compared with those from GC coupled with electron ionization high-resolution mass spectrometry (El-HRMS). Clean-up procedures for analysis of OH-PCBs homologs in liver and brain samples involve a pretreatment step consisting of acetonitrile partition and 5% hydrated silica-gel chromatography before derivatization. Recovery rates of tri- and tetra-chlorinated OH-PCBs in the acetonitrile partition method followed by the 5% hydrated silica-gel column (82% and 91%) were higher than conventional sulfuric acid treatment (2.0% and 3.5%). The method detection limits of OH-PCBs for each matrix obtained by GC/ECNI-MS and GC/EI-HRMS were 0.58-2.6 pg g(-1) and 0.36-1.6 pg g(-1) wet wt, respectively. Recovery rates of OH-PCB congeners in spike tests using sample matrices (10 and 50 pg) were 64.7-117% (CV: 4.7-14%) and 70.4-120% (CV: 2.3-12%), respectively. This analytical method may enable the simultaneous detection of various OH-PCBs from complex tissue matrices. Furthermore, this method allows more comprehensive assessment of the biological effects of OH-PCB exposure on critical organs. (C) 2013 Elsevier B.V. All rights reserved.
  • Mari Ochiai, Kei Nomiyama, Tomohiko Isobe, Hazuki Mizukawa, Tadasu K. Yamada, Yuko Tajima, Takashi Matsuishi, Masao Amano, Shinsuke Tanabe
    CHEMOSPHERE 92 7 803 - 810 2013年08月 [査読有り]
     研究論文(学術雑誌) 
    The present study investigated polychlorinated biphenyls (PCBs) and hydroxylated metabolites of PCBs (OH-PCBs) in blood from three porpoise species: finless porpoises (Neophocaena phocaenoides), harbor porpoises (Phocoena phocoena), and Dall's porpoises (Phocoenoides dalli). The porpoises were found stranded or were bycaught along the Japanese coast. Concentrations of OH-PCB were the highest in Dall's porpoises (58 pg g(-1) wet wt), second highest in finless porpoises (20 pg g(-1) wet wt), and lowest in harbor porpoises (8.3 pg g(-1) wet wt). The concentrations in Dall's porpoises were significantly higher than the concentrations in finless porpoises and harbor porpoises (p < 0.05 and p < 0.01, respectively). There was a positive correlation between PCB and OH-PCB concentrations (r = 0.67, p < 0.001), suggesting the possible concentration-dependent induction of CYP enzymes. The three porpoise species may have exceptionally low metabolic capacities compared with other marine and terrestrial mammals, because low OH-PCB/PCB concentration ratios were found, which were 0.0016 for Dall's porpoises, 0.0013 for harbor porpoises, and 0.00058 for finless porpoises. Distinct differences in the OH-PCB congener patterns were observed for the three species, even though they are taxonomically closely related. (c) 2013 Elsevier Ltd. All rights reserved.
  • Kenshi Sankoda, Tomonori Kuribayashi, Kei Nomiyama, Ryota Shinohara
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 47 13 7037 - 7044 2013年07月 [査読有り]
     研究論文(学術雑誌) 
    In this study, we hypothesize that natural photochemical reactions of polycyclic aromatic hydrocarbons (PAHs) in tidal flats are responsible for the occurrence of chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs). This study aims to survey the impact of photochemical reactions using a combination of field surveys and lab-scale experiments. Concentrations and profiles of PAHs and Cl-PAHs in road dust and sediments collected from seven tunnels and two watersheds, respectively, were determined. In the lab-scale experiments, anthracene was irradiated with ultraviolet (UV) light under various salinity conditions. No detectable Cl-PAHs were found in the road dust. However, Cl-PAHs were detected in the sediments from 700 to 6.1 x 103 pg g-1 and speClfically from downstream sites. 2-Monochloroanthracene (2-Cl-ANT) and 9,10-dichloroanthracene (9,10-di-Cl-ANT) were dominant in the sediments. In the IDomen River watershed, the Sigma Cl-PAHs and the salinity showed a significant positive correlation (p < 0.01) in the sediments, while such a correlation was not found for PAHs. 2-Cl-ANT, 9-monochloroanthracene, and 9,10-di-Cl-ANT were identified as transformation products in the UV irradiation experiments. Production of these Cl-PAHs was dependent on the solution salinity. These results support our hypothesis, and we conclude that photochemical reactions significantly contribute to the occurrence of Cl-PAlis in the studied tidal flats.
  • Kenshi Sankoda, Kei Nomiyama, Tomonori Kuribayashi, Ryota Shinohara
    POLYCYCLIC AROMATIC COMPOUNDS 33 3 236 - 253 2013年05月 [査読有り]
     研究論文(学術雑誌) 
    Selected six kinds of polycyclic aromatic hydrocarbons (PAHs) (naphthalene: NA, phenanthrene: (PHE), anthracene (ANT), fluoranthene (FLR), pyrene (PYR), and benzo[a]pyrene (B[a]P)) were exposed to natural sunlight under simulated tidal flat conditions, to check possibilities for photochemical productions of halogenated derivatives in the coastal environment. Moreover, 11 molecular descriptors were derived based on PM6 Hamiltonian for each PAH. Photochemical reaction induced productions of various 13 kinds of halogenated derivatives, which include 7 unknown products. In the determined 6 halogenated derivatives, the highest production amount was 6-chlorobenzo[a]pyrene. Moreover, PYR showed the behavior that produced halogenated derivatives significantly higher than its structural isomer of FLR. After the determination of production amounts, correlation between the production amounts of halogenated derivatives and the molecular descriptors of individual PAHs were examined. The energy gap between the highest occupied molecular orbital and the lowest unoccupied molecular orbital (E-HOMOLUMO), which is a measure of chemical stability, showed the strongest significance (p < 0.01) and give enough explanation for differences in the production amounts among structural isomers. PAHs that have small E-HOMOLUMO showed high potential to produce halogenated derivatives. ANT, PYR and B[a]P would have potentially high capability to induce halogenated derivatives in the coastal environment. We further discussed the impact of photochemical reaction on environmental fate of PAHs and halogenated PAHs. Congener profiles observed in our previous field surveys seemed to suggest contributions of photochemical reaction to occurrences of Cl-PAHs.
  • Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Shuuji Yachimori, Terutake Hayashi, Yutaka Tashiro, Yasuko Nagano, Shinsuke Tanabe
    ENVIRONMENTAL POLLUTION 174 28 - 37 2013年03月 [査読有り]
     研究論文(学術雑誌) 
    Residue levels and patterns of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), their hydroxylated metabolites (OH-PCBs, OH-PBDEs), and methoxylated PBDEs (MeO-PBDEs) in the blood of various terrestrial mammals in Japan, including cats, raccoon dogs, dogs, masked palm civets, foxes, raccoons, badgers, and mongooses were determined. Tri- through penta-chlorinated OH-PCBs were predominant in cat blood, whereas hexa- through octa-chlorinated OH-PCBs were found in other species. High proportion of BDE209 was found in all species, suggesting exposure to municipal waste and soil containing higher levels of deca-BDE products. 6OH-/MeO-BDE47 and 2'OH-/MeO-BDE68 were dominant in all terrestrial mammals. This is first report on the detection of OH-/MeO-PBDEs in the blood of terrestrial mammals. High concentrations of OH-/MeO-PBDEs were found in cats, suggesting the intake of these compounds from seafood. Cats exhibited higher accumulation and specific patterns of OH-PCBs, OH-PBDEs, and MeO-PBDEs, they may be at a high risk from these metabolites. (C) 2012 Elsevier Ltd. All rights reserved.
  • Akifumi Eguchi, Kei Nomiyama, Gnanasekaran Devanathan, Annamalai Subramanian, Kesav A. Bulbule, Peethambaram Parthasarathy, Shin Takahashi, Shinsuke Tanabe
    ENVIRONMENT INTERNATIONAL 47 8 - 16 2012年10月 [査読有り]
     研究論文(学術雑誌) 
    We determined the contamination status and accumulation profiles of polychlorinated biphenyls (PCBs), hydroxylated PCB congeners (OH-PCBs), polybrominated diphenyl ethers (PBDEs), hydroxylated PBDEs (OH-PBDEs), methoxylated PBDEs (MeO-PBDEs), and bromophenols (BPhs) in serum from e-waste recycling workers and residents near a coastal area in India. Residue levels of penta- to octa-chlorinated PCBs, penta- to octa-chlorinated OH-PCBs, 6MeO-BDE47, 6OH-BDE47, and 2,4,6-tri-BPh in serum from residents living near the coastal area were significantly higher than those in serum from e-waste recycling workers. Residue levels of tri- to tetra-chlorinated PCBs, tri- to tetra-chlorinated OH-PCBs. PBDEs. octa-brominated OH-PBDEs, and tetra-BPhs in serum from e-waste recycling workers were higher than those in serum from residents living near the coastal area. Principal component analysis revealed that residents living near the coastal area and e-waste recycling workers had different serum profiles of chlorinated and brominated compounds. (C) 2012 Elsevier Ltd. All rights reserved.
  • Rumi Tanoue, Yuri Sato, Mild Motoyama, Shuhei Nakagawa, Ryota Shinohara, Kei Nomiyama
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 41 10203 - 10211 2012年10月 [査読有り]
     研究論文(学術雑誌) 
    Land application of recycled manure produced from biosolids and reclaimed wastewater can transfer pharmaceutical chemicals to terrestrial environments, giving rise to potential accumulation of these residues in edible plants. In this study, the potential for plant uptake of 13 pharmaceutical chemicals, and the relation between the accumulation features within the plant and the physicochemical properties were examined by exposing pea and cucumber to an aqueous solution containing pharmaceutical chemicals. Ten of 13 compounds tested were detected in plant leaves and stems. Comparison of the plant uptake characteristics and the octanol water partition coefficient of pharmaceutical chemicals showed that compounds with an intermediate polarity such as carbamazepine and crotamiton could be easily transported to plant shoots. Moreover, these results suggest the possibility of highly hydrophilic pharmaceutical chemicals such as trimethoprim and sulfonamides to be accumulated in plant roots owing to their low permeability in root cell membranes.
  • Kenshi Sankoda, Kei Nomiyama, Takayuki Yonehara, Tomonori Kuribayashi, Ryota Shinohara
    CHEMOSPHERE 88 5 542 - 547 2012年07月 [査読有り]
     研究論文(学術雑誌) 
    This study investigated environmental distributions and production mechanisms of chlorinated polycyclic aromatic hydrocarbons (CI-PAHs) in the sediments from some tidal flats located in Asia. CI-PAHs were found in sediments taken from Arao tidal flat, Kikuchigawa River and Shirakawa River. The range of Sigma CI-PAHs was from 25.5 to 483 pg g(-1) for Kikuchigawa River and Arao tidal flat, respectively. Concentrations of PAHs and CI-PAHs showed no significant correlations (r = 0.134). This result suggests that the origins of these compounds differ. In the identified CI-PAH isomers, the most abundant CI-PAH isomer was 9,10-dichloroanthracene (9,10-di-CI-ANT) in the three sites. In general, concentrations of CI-ANTs in the coastal environment are about 3-5 orders of magnitude lower than those of anthracene (ANT). However, concentration ratios between CI-ANTs and ANT (CI-ANTs/ANT) in the sediments ranged from 4.1% to 24.6%. This result indicated that CI-PAHs were not generated under industrial processes but the high concentration ratios have resulted from the contribution of photochemical production of CI-ANTs in the sediments because ANT is known to have high photochemical reactivity. For examining this phenomenon, ANT adsorbed onto glass beads was irradiated with UV under the mimicked field conditions of tidal flats. As a result, it was noticed that, while chlorinated derivatives were negligible in a light-controlled group, production of 2-CI-ANT, 9-CI-ANT and 9,10-diCI-ANT on the irradiated surface were found in this study. These results suggest that photochemical reaction of PAHs can be a potential source of the occurrence of CI-PAHs in the coastal environment. (C) 2012 Elsevier Ltd. All rights reserved.
  • Inter-species Differences of Hydroxylated Polychlorinated Biphenyls (OH-PCBs) in the Blood of Small Cetaceans.
    Ochiai M, Nomiyama K, Isobe T, Matsuishi T, Yamada T.K, Tanabe S
    Interdisciplinary Studies on Environmental Chemistry 5 213 - 220 2012年 [査読有り]
     
    Proceedings
  • 生活関連化学物質による水環境汚染: 医薬品類による河川の汚染実態
    野見山桂, 田上瑠美, 篠原亮太
    産業と環境 8 37 - 41 2012年 [招待有り]
     研究論文(学術雑誌) 
    総説
  • Kei Nomiyama, Akifumi Eguchi, Hazuki Mizukawa, Mari Ochiai, Satoko Murata, Masayuki Someya, Tomohiko Isobe, Tadasu K. Yamada, Shinsuke Tanabe
    ENVIRONMENTAL POLLUTION 159 12 3364 - 3373 2011年12月 [査読有り]
     研究論文(学術雑誌) 
    We determined the residue levels and patterns of hydroxylated polybrominated diphenyl ethers (OH-PBDEs), and related compounds, such as PBDEs, methoxylated PBDEs (MeO-PBDEs), and bromophenols (BPhs) in the blood of eleven cetacean species stranded along the Japanese coasts. The dominant OH- and MeO-PBDE isomers found in all cetaceans were 6OH-BDE47 and 6MeO-BDE47. Additionally, 2,4,6-triBPh was dominant isomer in all cetaceans. In contrast, specific differences in the distribution of para- and meta- OH-PBDE isomers and some BPhs (potential PBDEs metabolites) were found among the cetaceans. Residue levels of Sigma MeO-PBDEs and 6OH-BDE47 + 2'OH-BDE68, and 2,4,6-triBPh and 6OH-BDE47 + 2'OH-BDE68 showed a significant positive correlation. These results may suggest that the large percentages of OH-PBDEs, MeO-PBDEs and 2,4,6-triBPh might share common source (i.e. biosynthesis by marine organisms), or metabolic pathway in cetacean species. Significant correlations were found between the concentrations of BDE99 and 2,4,5-triBPh. This result suggested that 2,4,5-triBPh in cetaceans could be a metabolite of BDE99. (C) 2011 Elsevier Ltd. All rights reserved.
  • Kei Nomiyama, Yukiko Uchiyama, Satoko Horiuchi, Akifumi Eguchi, Hazuki Mizukawa, Sawako Horai Hirata, Ryota Shinohara, Shinsuke Tanabe
    CHEMOSPHERE 85 3 315 - 321 2011年10月 [査読有り]
     研究論文(学術雑誌) 
    Information on accumulation of polychlorinated biphenyl metabolites (OH-PCBs) and hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in the blood of marine fish is limited. The present study, we determined the residue levels and patterns of PCBs, OH-PCBs, PBDEs, OH-PBDEs and methoxylated PBDEs (MeO-PBDEs) in the blood collected from scalloped hammerhead shark (Sphyrna lewini) and Japanese amberjack (Seriola quinqueradiata), species of predatory fish at Japanese coastal waters. The predominant homologues found in Japanese amberjacks were mono- and di-chlorinated OH-PCBs, and scalloped hammerhead sharks were octa-chlorinated OH-PCBs. The predominant OH-PCB isomers were lower-chlorinated OH-PCBs such as 6OH-CB2 and 2'OH-CB9 in Japanese amberjacks. This result suggests that exposure of Japanese amberjacks to lower-chlorinated OH-PCBs might be from the ambient aquatic environment. In scalloped hammerhead sharks, 4,4'diOH-CB202, 4OH-CB201 and 4OH-CB146 were the predominant isomers accounting for approximately 60% of the total OH-PCBs. The predominant MeO-PBDE isomers were 6MeO-BDE47 followed by 2'MeO-BDE68 in both species. As for OH-PBDE isomers, 6OH-BDE47 was predominant followed by 2'OH-BDE68 in Japanese amberjacks and scalloped hammerhead sharks. Residue levels of Sigma MeO-PBDEs and Sigma OH-PBDEs showed a significant positive correlation (p = 0.029). This result suggests that MeO-PBDEs and OH-PBDEs share a common source or a metabolic pathway in fishes. Characteristic differences found in the profiles of OH-PCBs and OH-PBDEs in Japanese amberjack and scalloped hammerhead shark show the need for further studies on the differences in exposure profiles, metabolic capacities and toxic effects in fish. (C) 2011 Elsevier Ltd. All rights reserved.
  • Miki Motoyama, Shuhei Nakagawa, Rumi Tanoue, Yuri Sato, Kei Nomiyama, Ryota Shinohara
    CHEMOSPHERE 84 4 432 - 438 2011年07月 [査読有り]
     研究論文(学術雑誌) 
    In recent years, sludge generated in sewage treatment plants (STPs) and solid waste from livestock being utilized is useful for circulation of nourishment in farmlands as recycled organic manure (ROM). In this study, we determined the residue levels and patterns of 12 pharmaceutical products generated by human activity in the ROMs produced from human waste sludge (HWS), sewage sludge (SS), cattle manure (CM), poultry manure (PM), swine manure (SM) and horse manure (HM). The kind and number of pharmaceutical products detected in ROMs were different. Fluoroquinolones (FQs) were detected at high levels in HWS and SS samples. In addition, the detection frequency and concentration levels of sulfonamides (SAs) in PM and SM were high. Moreover, high concentrations of chlortetracycline (CTC) were found in only SM. These differences reflect specific adherence adsorption of the pharmaceutical products to different livestock and humans. Moreover, it was found that the concentrations of pharmaceutical products and fermentation levels of ROMs had significant positive correlation (r = 0.41, p = 0.024). When the fermentation test of ROM was conducted in a rotary fermentor in a lab scale test, the residue levels of pharmaceutical products decreased effectively except carbamazepine (CBZ). The rates of decrease were in the case of tetracyclines (TCs): 85-92%, FQs: 81-100%, erythromycine: 67%, SAs: 79-95%, trimethoprim: 86% and CBZ: 37% by 30 d. Pharmaceutical products that can be decomposed by fermentation process at the lowest impact of residual antibiotic activities may therefore be considered as environmentally friendly medicines. (C) 2011 Elsevier Ltd. All rights reserved.
  • K. Sankoda, H. Matsuo, M. Ito, K. Nomiyama, K. Arizono, R. Shinohara
    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 86 5 470 - 475 2011年05月 [査読有り]
     研究論文(学術雑誌) 
    The photodegradation pathways of 2-(2,4-dichlorophenoxy)-5-chlorophenol (triclosan) in water were studied. The main purposes were to identify structures of intermediates derived by radical reaction using TiO2 advanced oxidation processes and to evaluate the endocrine disrupting activities in treated triclosan during oxidative reactions. Intermediates such as dichlorophenols, 2,8-dibenzo-p-dioxin, tetrachlorinated diphenyl ether (tetraclosan) and hydroxylated triclosan were produced by photoreaction. The estrogen, thyroid hormone and retinoid X receptor activities of the treated triclosan were measured with the yeast two-hybrid assay. It was found that tetraclosan and 2,4-dichlorophenol have stronger thyroid hormone activities than triclosan in the presence of S9.
  • Organohalogen Contaminants and their Hydoxylated Metabolites in the Blood of Japanese Terrestrial Mammals.
    Mizukawa H, Nomiyama K, Nakatsu S, Yachimori S, Hayashi T, Tashiro Y, Tanabe S
    Organohalogen Compounds 73 1207 - 1210 2011年 [査読有り]
     
    Proceedings
  • Kei Nomiyama, Satoko Murata, Tatsuya Kunisue, Tadasu K. Yamada, Hazuki Mizukawa, Shin Takahashi, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 44 10 3732 - 3738 2010年05月 [査読有り]
     研究論文(学術雑誌) 
    In this study, we determined the residue levels and patterns of polychlorinated biphenyls (PCBs) and hydroxylated PCBs (OH-PCBs) in the blood from eight species of toothed whales and three species of baleen whales stranded along the Japanese coast during 1999-2007. Penta- through hepta-chlorinated PCB congeners were the dominant homologue groups in all cetaceans. In contrast, specific differences in the distribution of dominant OH-PCB isomers and homologues were found among the cetacean species. In five species of toothed whales (melon-headed whale, Stejneger's beaked whale, Pacific white-sided dolphin, Blainville's beaked whale, and killer whale), the predominant homologues were OH-penta-PCBs followed by OH-tetra-PCBs and OH-tri-PCBs. The predominant homologues of finless porpoise and beluga whale were OH-penta-PCBs followed by OH-hexa-PCBs and OH-tri-PCBs. The predominant OH-PCB isomers were para-OH-PCBs such as 4'OH-CB26, 4'OH-CB25/4'OH-CB26/4OH-CB31, 4OH-CB70, 4'OH-CB72, 4'OH-CB97, 4'OH-CB101/4'OH-CB120, and 4OH-CB107/4'OH-CB108 in toothed whales. In three baleen whales (common minke whale, Bryde's whale, and humpback whale) and in sperm whale (which is a toothed whale), OH-octa-PCB (4OH-CB202) was the predominant homologue group accounting for 40-80% of the total OH-PCB concentrations. The differences in concentrations and profiles of OH-PCBs may suggest species-specific diets, metabolic capability, and the transthyretin (TTR) binding specificity. These results reveal that the accumulation profiles of OH-PCBs in cetacean blood are entirely different from the profiles found in pinnipeds, polar bear, and humans.
  • Kei Nomiyama, Tomonori Yonehara, Saori Yonemura, Megumi Yamamoto, Chihaya Koriyama, Suminori Akiba, Ryota Shinohara, Minoru Koga
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 44 8 2890 - 2896 2010年04月 [査読有り]
     研究論文(学術雑誌) 
    In the present study, we analyze and characterize the levels of PCBs and OH-PCBs in serum and breast adipose tissue, and measure TH (total 3,5,5'-triiodothyronine (tT3) and total L-thyroxine (tT4)) levels in serum of Japanese women diagnosed with breast cancer (n = 51) to know how PCBs affect thyroid hormone homeostasis. Concentrations of Sigma(90)OH-PCBs in serum and adipose tissues were 630 pg g(-1) serum wet wt. and 140 pg/g(-1) lipid wt., respectively, and the levels of OH-PCBs in adipose tissues were 4-5 times lower than in serum. The tT3 and tT4 concentrations in serum (n = 20) were 1.3 ng g(-1) serum wet wt. and 62 ng g(-1) serum wet wt., respectively. Among all the OH-PCBs, 4'-OH-CB101/120, 4-OH-CB107/4'-OH-CB108, 3'-OH-CB138, 4-OH-CB146, 3-OH-CB153, 4'-OH-CB172, and 4-OH-CB187 were the dominant isomers in serum. No significant correlation between the concentrations of the TH and OH-PCBs in serum was observed (p > 0.05). The results indicated that the relationship between the OH-PCB levels and the thyroid hormone markers was rare. From analysis of two kinds of human biological samples, it was estimated that OH-PCBs have little effect on thyroid hormone homeostasis.
  • Kei Nomiyama, Yuji Nomura, Tohru Takahashi, Yukiko Uchiyama, Koji Arizono, Ryota Shinohara
    CHEMOSPHERE 78 7 800 - 806 2010年02月 [査読有り]
     研究論文(学術雑誌) 
    Vitellogenin (VTG)-inducing activities of 21 kinds of hydroxylated polychlorinated biphenyls (OH-PCBs) were investigated by an assay system using the primary-cultured hepatocyte of the adult male Xenopus laevis. An enzyme-linked immunoabsorbent assay (ELISA) was able to detect VTG at a minimum detection limit of 0.2 ng mL(-1). The levels of VTG production in hepatocytes of male X laevis exposed to six kinds of OH-PCB isomers (2'-OH-CB30, 3'-OH-CB30, 4'-OH-CB30, 4'-CH-CB50, 4'-OH-CB61 4'-OH-CB65) were significantly higher as compared to the control group (p < 0.05). The relative ranking of the OH-PCBs was E2 (100%), 4'-OH-CB30 (2.0%) > 2'-OH-CB30 (0.15%) > 4'-OH-CB61 (0.046%) > 3'-OH-CB30 (0.013%) > 4'-OH-CB50 (0.002%) > 4'-OH-CB65 (0.0001%). Although there were some differences between the estrogenicity values by the VTG-inducing activities and yeast two-hybrid assay for human estrogen receptor alpha (ER alpha). The results from VTG-assays suggested that an important factor for determining estrogenicity is the positions of the hydroxyl group and chlorine with the highest activity resulting from a para-hydroxyl group. The OH-PCB structures of high rank order in the VTG-assay had no chlorine substituted phenolic ring. Moreover, the meta-chlorine substituted phenolic ring is an important factor in determining estrogenicity. The CH-PCBs with meta-chlorine substituted phenolic ring did not show estrogenicity during VTG-assay. (C) 2009 Elsevier Ltd. All rights reserved.
  • 下水汚泥・畜産廃棄物を原料とした再生肥料(コンポスト)中の医薬品の存在
    水環境学会誌 33 5 152 - 155 2010年 [査読有り]
  • S. Nakagawa, H. Matsuo, M. Motoyama, K. Nomiyama, R. Shinohara
    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 83 3 328 - 333 2009年09月 [査読有り]
     研究論文(学術雑誌) 
    The behavior of estrogens (estrone: E1, 17 beta-estradiol: E2, estriol: E3 and ethinylestradiol: EE2) and an androgen (testosterone) in the water and sludge from Johkasou in Japan was investigated. The concentrations of E1, E2, E3 and testosterone in water samples from the Johkasou were 33-500, N.D. similar to 150, N.D. similar to 6,700 and 500 ng/L, respectively. In sludge samples, the concentrations of E1, E2, E3, and testostrerone were N.D. similar to 39, N.D. similar to 6.7, N.D. similar to 60 and 0.2-9.0 ng/L, respectively. EE2 was not detected in all samples. The removal rates of E1, E2, E3 and testosterone in Johkasou were 45%-91%, 66%-100%, 90%-100%, and about 90%, respectively.
  • Hydroxylated polychlorinated biphenyls in the blood of Baikal seals (Pusa Sibirica)
    Imaeda, D, Kunisue, T, Iwata, H, Tsydenova, O, Takahashi, S, Nomiyama, K, Amano, M, Petrov, A.E, Batoev, B.V, Tanabe, S
    Organohalogen Compounds 70 1475 - 1478 2008年 [査読有り]
     
    Proceedings
  • Kei Nomiyama, Teiji Tanizaki, Toyokazu Koga, Koji Arizono, Ryota Shinohara
    ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 52 1 8 - 15 2007年01月 [査読有り]
     研究論文(学術雑誌) 
    The oxidative degradation behavior of bisphenol A (BPA) using titanium dioxide (TiO2) in water was investigated. The main purposes were to clarify the relationship with estrogenic activity from the degradation pathways demonstrated by oxidation of BPA in water. Approximately 99% of the BPA decomposed within 300 min, and gas chromatography (GC) mass spectrometry (MS) and liquid chromatography (LC) MS analysis revealed many intermediates during the degradation process. Intermediates by decomposition of BPA, such as hydroxylated-BPA (OH-BPA), carboxylic intermediates, phenolic intermediates, and other intermediates produced by the cleavage of a benzene ring were identified and quantified. Estrogenic activities of the degradation pathways of the BPA in water were assessed by using a constructed yeast two-hybrid assay system for human estrogen receptor alpha (hER alpha) and Japanese medaka fish (Oryzias latipes) estrogen receptor alpha (medER alpha). Estrogenic activity for hER alpha and medER alpha was reduced to less than 20% of the initial activity for BPA after 240 min of UV irradiation. However, estrogenic activity for medER alpha was increased by 110% from the initial activity for BPA at 60 min of UV irradiation. It was estimated that medER alpha assay was more sensitive for BPA and the intermediates than was the hER alpha assay. From these findings, we estimate that the intermediates by the oxidation of BPA have the behaviors of xenoestrogen to the aquatic wildlife in the environment.
  • JI Hezhe, ZHANG Daizhou, SHINOHARA Ryouta, FUJIIE Wataru, KOGA Minoru, SHI Zongbo, NOMIYAMA Kei
    Earozoru Kenkyu 22 2 135 - 142 2007年 [査読有り]
     研究論文(学術雑誌)
  • 酸化チタン光触媒を利用した水環境中におけるポリ塩化ビフェニル(PCBs)の酸化に伴う水酸化PCBs(OH-PCBs)の生成メカニズム: 生成するOH-PCBsの化学構造と内分泌攪乱性の解明
    野見山 桂, 有薗 幸司, 篠原 亮太
    水環境学会誌 212 - 217 2007年 [査読有り]
     研究論文(学術雑誌)
  • Kei Nomiyama, Teiji Tanizaki, Koji Arizono, Ryota Shinohara
    CHEMOSPHERE 66 6 1138 - 1145 2007年01月 [査読有り]
     研究論文(学術雑誌) 
    The oxidative photodegradation behaviors of selected three coplanar polychlorinated biphenyls (PCBs), (CB77, C1381, and C13169) using titanium dioxide (TiO2) in water were investigated. The main purposes were to clarify the structural relation between the original PCBs and the intermediates derived by TiO2 Oxidation and to evaluate the estrogenic and thyroid hormonal activity in the treated three coplanar PCBs during the oxidative reactions. Approximately 90% of the three coplanar PCBs decomposed within 180 min. Intermediates from the decomposition of the three coplanar PCBs, such as some hydroxylated-PCBs (OH-PCBs), carboxylic intermediates, phenolic intermediates, and other intermediates produced by the cleavage of a benzene ring were identified and quantified. In the degradation pathways, the produced amounts of OHPCBs increased within 60 min of irradiation time. The estrogenic activity and thyroid hormonal activity of the intermediates from the three coplanar PCBs in water at 0, 60, 120, 180 and 240 min of irradiation time were assessed by using a yeast two-hybrid assay system for human estrogen receptor alpha (hER alpha) and human thyroid hormone receptor alpha (hTR alpha). The maximal estrogenic activities were induced by the solutions of decomposed PCBs with irradiation time at 60-120 min similar and slightly lower than those after the irradiation time. We found that the solutions occuring during the irradiation times of 60-120 min contained several 4-OH-PCBs substituted with OH and Cl at para- and para'-positions having estrogenic activity. The thyroid hormonal activity was not detected in the decomposed three coplanar PCBs solutions. (c) 2006 Elsevier Ltd. All rights reserved.
  • 前田 絵梨子, 野見山 桂, 篠原 亮太
    環境化学 16 2 239 - 248 2006年 [査読有り]
     研究論文(学術雑誌)
  • 本山 充希, 古閑 豊和, 野見山 桂, 中川 修平, 松尾 英樹, 篠原 亮太
    環境化学 16 4 615 - 626 2006年 [査読有り]
     研究論文(学術雑誌)
  • K Nomiyama, T Tanizaki, H Ishibashi, K Arizono, R Shinohara
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 39 22 8762 - 8769 2005年11月 [査読有り]
     研究論文(学術雑誌) 
    The oxidative photodegradation behaviors of selected polychlorinated biphenyls (PCBs) [2,3,3',4'-tetraCB (BZ number: CB56), 2,3',4',5-tetra (CB70), and 3,4,4',5-tetraCB (CB81: coplanar PCB)] using titanium dioxide (TiO2) in water were investigated. The main purposes were to clarify the structural relation between the original PCBs and the intermediates derived by TiO2 oxidation and to evaluate the estrogenic activity in the treated PCBs during the oxidative reactions. Approximately 90% of the three tetraCBs decomposed within 120 min. Intermediates by decomposition of three tetraCBs, such as some OH-tetraCBs and OH-triCBs, carboxylic intermediates, phenolic intermediates, and other intermediates produced by the cleavage of a benzene ring were identified and quantified. In the degradation pathways, the produced amounts of OH-tetraCB and OH-triCB increased within 60 min of irradiation time. Estrogenic activities of the intermediates from the three tetraCBs in water were assessed by using a yeast two-hybrid assay system for human estrogen receptor alpha (hER alpha). The maximal estrogenic activities were induced by the solutions of decomposed CB81 with irradiation time at 60 min. We found that the solutions at an irradiation time of 60-120 min contained several 4-OH-tetraCBs and 4-OH-triCBs substituted with OH and Cl at para- and para'-positions. It is presumed that the chemical structures of the 4-OH-PCBs are similar to that of 17 beta-estradiol (beta-E2); these intermediates present strong estrogenic activities. Moreover, we learned that there is a high possibility of conversion from some low toxic PCBs congeners to strong estrogenic OH-PCBs.

書籍

  • Hazuki Mizukawa, Kei Nomiyama (担当:共著, 範囲:Biotransformation of Brominated Compounds by Pet Dogs and Cats)
    Springer Nature 2020年01月
  • シャチ生態ビジュアル百科 世界の海洋に知られざるオルカの素顔を追う
    水口博也編著 (担当:分担執筆, 範囲:シャチの体にたまる有害化学物質)
    2015年
  • Interdisciplinary Studies on Environmental Chemistry-Exploring Possibility of Setting-up ESBs in Developing countries
    TERRAPUB 2010年
  • Interdisciplinary Studies on Environmental Chemistry-Exploring Possibility of Setting-up ESBs in Developing countries
    TERRAPUB 2010年
  • Interdisciplinary Studies on Environmental Chemistry vol.2
    TERRAPUB 2008年
  • Persistent Organic Pollunant (POPs) Reseach in Asia.
    2008年
  • Interdisciplinary Studies on Environmental Chemistry vol.2
    TERRAPUB 2008年
  • Persistent Organic Pollunant (POPs) Reseach in Asia.
    2008年

講演・口頭発表等

  • 野生動物における有機ハロゲン化合物の代謝・動態と甲状腺ホルモンへの影響,  [招待講演]
    野見山 桂
    第20回日本毒性学会生涯教育講習会 2019年06月 公開講演,セミナー,チュートリアル,講習,講義等
  • 地球規模で考える環境汚染 医薬品と環境問題を考える  [招待講演]
    野見山 桂
    コープ 一般講演会 2019年02月 公開講演,セミナー,チュートリアル,講習,講義等
  • 自然写真を通して環境問題を考える  [招待講演]
    野見山 桂
    コミュニティ・カレッジ 環境講座 2019年01月 公開講演,セミナー,チュートリアル,講習,講義等
  • Contamination status of organohalogen compounds in pet cats, cat food and house dust from Thailand
    Shimasaki, M., Mizukawa, H., Takaguchi, K., Saengtienchai, A., Ngamchirttakul, A., Pencharee, D., Khidkhan, K., Ishizuka, M., Ikenaka, Y., Nakayama, S. M. M., Kunisue, T., Tanabe, S., Nomiyama, K.
    28th Symposium on Environmental Chemistry 2019年 口頭発表(一般)
  • ネオニコチノイドの母子間移行の実態と移行メカニズムの解明
    池中良徳, 一瀬貴大, ニマコ・コリンズ, 市川 剛, 野見山 桂, 長谷川 浩, 中山翔太, 星 信彦, 平 久美子, 石塚 真由美
    第28回環境化学討論会 2019年
  • デカブロモジフェニルエーテル(BDE209)の長期曝露がイエネコへ及ぼす影響
    田中啓介, 野見山 桂, 水川葉月, 高口倖暉, 田上瑠美, 横山 望, 市居 修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第28回環境化学討論会 2019年
  • PCBs 曝露によるネコ肝トランスクリプトームへの影響
    水川葉月, Hoa Thanh Ngyuen, 岩田久人, 野見山 桂, 池中良徳, 中山翔太, 横山 望, 市居 修, 滝口満喜, Kraisiri Khidkhan, 田辺信介, 石塚真由美
    第28回環境化学討論会 2019年
  • Toxicokinetic parameters causing the discrepancy between the measured and predicted bioconcentration factors of pharmaceuticals and personal care products in fish
    Tanoue, R., Nakayama, K., Kondo M., Nomiyama, K., Kunisue, T., Tanabe, S.
    Society of Environmental Toxicology and Chemistry (SETAC) Europe 29th Annual Meeting 2019年 ポスター発表
  • 極性汚染物質による高等動物の汚染実態とリスク評価の試み (ハロゲン化フェノール類とPPCPs汚染解析事例から)  [招待講演]
    野見山 桂
    酪農学園大学特別講義 2018年12月 公開講演,セミナー,チュートリアル,講習,講義等
  • Polychlorinated biphenyl exposure increased thyroid hormone excretion in dogs  [通常講演]
    野見山 桂
    2nd Chemical Hazard Symposium 2018年12月 シンポジウム・ワークショップパネル(指名)
  • Characterization of CYP genes related-PCBs exposure in cats  [通常講演]
    野見山 桂
    2nd Chemical Hazard Symposium 2018年12月 シンポジウム・ワークショップパネル(指名)
  • Comparison of effects on comprehensive gene expression by PCBs exposure in Cats and Dogs  [通常講演]
    野見山 桂
    2nd Chemical Hazard Symposium 2018年12月 シンポジウム・ワークショップパネル(指名)
  • 環境汚染化学物質の最前線  [通常講演]
    野見山 桂
    愛媛大学グローバルサイエンス(eGS) 2018年11月 公開講演,セミナー,チュートリアル,講習,講義等
  • Effects of PCB exposure on thyroid hormone levels in serum of dogs and cats.  [通常講演]
    野見山 桂
    SETAC North America 39th Annual Meeting 2018年11月 ポスター発表
  • Simultaneous determination of neurotransmitters and their precursors/metabolites in animal brain tissue.  [通常講演]
    野見山 桂
    SETAC North America 39th Annual Meeting 2018年11月 ポスター発表
  • 地球規模で考える環境汚染 ~ 環境汚染が私達の暮らしを壊す。 環境について考えてみませんか ~  [招待講演]
    野見山 桂
    コープ 一般講演会 2018年09月 公開講演,セミナー,チュートリアル,講習,講義等
  • 医薬品類およびパーソナルケア製品由来化学物質(PPCPs)による水圏生態系の汚染実態  [招待講演]
    野崎一茶, 田上瑠美, 野見山 桂, 国末達也, 田辺信介
    第21回日本水環境学会シンポジウム 2018年09月 シンポジウム・ワークショップパネル(指名)
  • PCBs as an environmental obesogen in dogs: evidence from hepatic transcriptome, metabolome, and lipidome analyses  [通常講演]
    野見山 桂
    38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018) 2018年08月 口頭発表(一般)
  • Pilot study on the toxicological assessment of organohalogen compounds in pet cat (Felis catus) serum using metabolomics approach  [通常講演]
    野見山 桂
    38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018) 2018年08月 口頭発表(一般)
  • Tissue distribution and CYP expression related-PCBs exposure in cats.  [通常講演]
    野見山 桂
    38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018) 2018年08月 ポスター発表
  • Contamination status and global comparison of organohalogen compounds and their related compounds in the pet cats in Thailand.  [通常講演]
    野見山 桂
    38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018) 2018年08月 ポスター発表
  • Effects of polychlorinated biphenyls (PCBs) in cats: Exhaustive gene expression analysis approach.  [通常講演]
    野見山 桂
    38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018) 2018年08月 口頭発表(一般)
  • ポリ塩化ビフェニル曝露によるイヌ・ネコの甲状腺ホルモン恒常性への影響  [通常講演]
    高口倖暉, 野見山 桂, 西川博之, 水川葉月, 田上瑠美, 草木桃子, 横山 望, 市居 修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 岩田久人, 国末達也, 田辺信介
    第45回日本毒性学会学術年会 2018年07月 ポスター発表
  • 3) 薬物・環境化学物質の代謝活性化と甲状腺ホルモンへの影響  [招待講演]
    野見山 桂
    環境ホルモン学会第 32 回講演会 2018年06月 公開講演,セミナー,チュートリアル,講習,講義等
  • 沖縄本島南部の河川水における医薬品類およびパーソナルケア製品由来化学物質の存在  [通常講演]
    田上瑠美, 国末達也, 向井幸乃, 後藤哲智, 田代 豊, 野見山 桂, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般)
  • LC-MS/MSを用いた脳中甲状腺ホルモンの高感度分析法の開発  [通常講演]
    小野純華, 高口倖暉, 田上瑠美, 国末達也, 田辺信介, 野見山 桂
    第27回環境化学討論会 2018年05月 口頭発表(一般)
  • 有機ハロゲン化合物曝露によるネコの生化学マーカーの変化および甲状腺機能に及ぼす影響評価  [通常講演]
    水川葉月, Khidkhan. K, 高口倖暉, 横山 望, 市居 修, 滝口満喜, 笹岡一慶, 池中良徳, 中山翔太, Tsendayush, S, 野見山 桂, 田辺信介, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般)
  • 野生哺乳類における硫酸転移酵素の動物種差解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山 桂, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般)
  • タイ王国におけるペットネコ血清およびペットフード中有機ハロゲン化合物と類縁化合物の汚染実態  [通常講演]
    島﨑真琴, 野見山 桂, 水川葉月, Saengtienchai, A, Ngamchirttakul, A, Pencharee, D, 星野光希, 山本恭央, 高口倖暉, 石塚真由美, 池中良徳, 中山翔太, 国末達也, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般)
  • ネコのin vivo BDE209 長期曝露試験による体内動態の解明および甲状腺ホルモンへの影響評価  [通常講演]
    田中啓介, 野見山 桂, 水川葉月, 高口倖暉, 田上瑠美, 横山 望, 市居 修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般)
  • 医薬品類およびパーソナルケア製品由来物質 (PPCPs) によるアジア途上国の水環境汚染と水生生物への影響評価  [通常講演]
    野崎一茶, 田上瑠美, 野見山 桂, 国末達也, 田辺信介, 磯部友彦
    日本水環境学会年会 2018年03月 口頭発表(一般)
  • 自然写真を通して環境問題を考える  [招待講演]
    野見山 桂
    愛媛大学校友会人材活用センター講演会 2018年03月 公開講演,セミナー,チュートリアル,講習,講義等
  • 哺乳類の残留する有機ハロゲン代謝物の脳移行とリスク評価への試み  [招待講演]
    野見山 桂
    第19回環境ホルモン学会研究発表会「若手の会キックオフシンポジウム」ー若手研究者のキャリア・ライフプランのためのReferencesー 2017年12月 シンポジウム・ワークショップパネル(指名)
  • 野生生物に残留するハロゲン化フェノール類の蓄積特性と毒性影響評価  [招待講演]
    野見山 桂
    第5回バイオシグナル研究会「生命環境と生物シグナル応答-基礎から応用まで-」 2017年10月 シンポジウム・ワークショップパネル(指名)
  • Persistent organic pollutants (POPs) in dolphin species  [通常講演]
    Ochiai, M, Isobe, T, Tajima, Y, Nomiyama, K, Kunisue, T, Iwata, H, Tanabe, S
    10th International Meeting of Asian Society of Conservation Medicine 2017年10月 口頭発表(一般)
  • Molecular mechanisms underlying species differences in transactivation potencies of seal and dog estrogen receptors by OH-PCBs.  [通常講演]
    Yoshinouchi, Y, Hirano, M, Nomiyama, K, Tanabe, S, Kim, E. Y, Iwata, H
    The 7th Joint Forum of Environmental Sciences 2017 2017年09月 ポスター発表
  • Contamination status and risk assessment of pharmaceuticals and personal care products (PPCPs) in freshwater fish from Asian developing countries.  [通常講演]
    Nozaki, K, Tanoue, R, Nomiyama, K, Subramanian, An, Bulbule, K. A, Parthasarathy, P, Viet, P. H, Tuyen, L. H, Sudaryanto, A, Isobe, T, Kunisue, T, Tanabe, S
    The 7th Joint Forum of Environmental Sciences 2017 2017年09月 ポスター発表
  • Occurrence of 72 pharmaceuticals and personal care products (PPCPs) in surface water from India, Indonesia, and Japan.  [招待講演]
    Tanoue, R, Nozaki, K, Nomiyama, K, Subramanian, An, Bulbule, K. A, Parthasarathy, P, Sudaryanto, A, Isobe, T, Kunisue, T, Tanabe, S
    The 7th Joint Forum of Environmental Sciences 2017 2017年09月 口頭発表(一般)
  • Serum biochemical responses in cats exposed to organohalogen compounds.  [通常講演]
    Kraisiri, K, Mizukawa, H, Ikenaka, Y, Nakayama, S. M. M, Nomiyama, K, Yokoyama, N, Sasaoka, K, Ichii, O, Takiguchi, M, Nishikawa, H, Tanaka, K, Takaguchi, K, Tanabe, S, Ishizuka, M
    Chemical Hazard Symposium (Joint Seminar on Environmental Chemistry and Toxicology) 2017年08月 口頭発表(一般)
  • PCB-induced disruption of thyroid hormone homeostasis in dogs and cats. Chemical Hazard Symposium  [通常講演]
    Takaguchi, K, Nomiyama, K, Nishikawa, H, Mizukawa, H, Tanoue, R, Yoshinouchi, Y, Yokoyama, N, Ichii, O, Takiguchi, M, Nakayama, S, Ikenaka, Y, Ishizuka, M, Iwata, H, Kunisue, T, Tanabe, S
    Chemical Hazard Symposium (Joint Seminar on Environmental Chemistry and Toxicology) 2017年08月 口頭発表(一般)
  • Evolution and enzymatic activity of Phase II drug metabolism enzymes in Carnivores.  [通常講演]
    Kondo, T, Ikenaka, Y, Nakayama, S. M. M, Kawai, Y. K, Mizukawa, H, Mitani, Y, Nomiyama, K, Tanabe, S, Ishizuka, M
    Chemical Hazard Symposium (Joint Seminar on Environmental Chemistry and Toxicology) 2017年08月 口頭発表(一般)
  • Uptake and metabolism of opioid analgesic tramadol in fish, and a linkage between internal concentrations and behavioural effects.  [通常講演]
    Tanoue, R, Margiotta-Casaluci, L, Huerta, B, Runnalls, T. J, Nomiyama, K, Kunisue T, Tanabe, S, Sumpter, J. P
    Chemical Hazard Symposium (Joint Seminar on Environmental Chemistry and Toxicology) 2017年08月 口頭発表(一般)
  • Exploration of lead exposure related biological pathways in Vietnamese acid-lead battery recycling workers.  [通常講演]
    Eguchi, A, Nomiyama, K, Sakurai, K, Trang, P, Viet, P, Takahashi, S, Iwata, H, Tanabe, S, Todaka, E, Mori, C
    Chemical Hazard Symposium (Joint Seminar on Environmental Chemistry and Toxicology) 2017年08月 口頭発表(一般)
  • DecaBDE might be associated with inhibition of thyroid hormone levels in serum of Japanese cats.  [通常講演]
    野見山 桂
    International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 口頭発表(一般)
  • Identification for the biotransformation of BDE209 by in vivo toxicokinetic analysis in Felis catus.  [通常講演]
    Tanaka, K, Nomiyama, K, Mizukawa, H, Takaguchi, K, Tanoue, R, Saengtienchai, A, Sainnoxoi, T, Yokoyama, N, Ichii, O, Takiguchi, M, Nakayama, S, Ikenaka, Y, Ishizuka, M, Kunisue, T, Tanabe, S
    International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 ポスター発表
  • Tissue distribution of polychlorinated biphenyls and their metabolites in dog and cat.  [通常講演]
    Takaguchi, K, Nomiyama, K, Nagano, Y, Mizukawa, H, Nakatsu, S, Kunisue, T, Tanabe, S
    International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 口頭発表(一般)
  • In vivo exposure to PCBs in cats: Analysis of metabolic capacities and effects on the thyroid hormone homeostasis.  [通常講演]
    Mizukawa, H, Nomiyama, K, Nishikawa, H, Maehara, M, Takai, N, Yokoyama, N, Ichii, O, Takiguchi, M, Ikenaka, Y, Shouta, M. M, Takaguchi, K, Tanabe, S, Ishizuka, M
    International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 口頭発表(招待・特別)
  • Pharmaceutical and personal care products in freshwater fish from Asian developing countries.  [通常講演]
    Nozaki, K, Tanoue, R, Nomiyama, K, Subramanian, A, Bulbule, K. A, Parhasarathy, P, Viet, P. H, Tuyen, L. H, Sudaryanto, A, Isobe, T, Kunisue, T, Tanabe, S
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 ポスター発表
  • Organophosphate flame retardants in wild birds – development of analytical methods and interspecies comparison.  [通常講演]
    Fumoto, M, Tue, N. M, Goto, A, Nakatsu, S, Nomiyama, K, Tanabe, S, Kunisue, T
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 ポスター発表
  • Effects of PCB-exposure on thyroid hormone homeostasis of dogs and cats.  [通常講演]
    Takaguchi, K, Nomiyama, K, Nishikawa, H, Mizukawa, H, Tanoue, R, Yoshinouchi, Y, Yokoyama, N, Ichii, O, Takiguchi, M, Nakayama, S, Ikenaka, Y, Ishizuka, M, Iwata, H, Kunisue, T, Tanabe, S
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 ポスター発表
  • Identification for the biotransformation of BDE209 by in vivo toxicokinetic analysis in Felis catus.  [通常講演]
    Tanaka, K, Nomiyama, K, Mizukawa, H, Takaguchi, K, Tanoue, R, Saengtienchai, A, Sainnoxoi, T, Yokoyama, N, Ichii, O, Takiguchi, M, Nakayama, S. Ikenaka, Y, Ishizuka, M, Iwata, H, Kunisue, T, Tanabe, S
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 ポスター発表
  • Investigation of biochemical changes in cats exposed to organohalogen compounds.  [通常講演]
    Kraisiri, K, Mizukawa, H, Nomiyama, K, Ikenaka, Y, Nakayama, S. M. M, Yokoyama, N, Sasaoka, K, Ichii, O, Takiguchi, M, Nishikawa, H, Tanaka, K, Takaguchi, K, Tanabe, S, Ishizuka, M
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 口頭発表(一般)
  • Uptake and metabolism of human pharmaceuticals by fish – a case study with opioid analgesic tramadol.  [通常講演]
    Tanoue, R, Margiotta-Casaluci, L, Huerta, B, Runnalls, T. J, Nomiyama, K, Tanabe, S, Sumpter, J. P
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 口頭発表(一般)
  • UDP-glucuronosyltransferase (UGT) among pinnipedia and other carnivores.  [通常講演]
    Kondo, T, Ikenaka, Y, Nakayama, S. M. M, Kawai, Y. K, Mizukawa, H, Mitani, Y, Nomiyama, K, Tanabe, S, Ishizuka, M
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 口頭発表(一般)
  • Structure-activity relationships of Bikal seal estrogen receptors and environmental pollutants.  [通常講演]
    Yoshinouchi, Y, Hirano, M, Nakata, H, Nomiyama, K, Tanabe, S, Kim, E. Y, Iwata, H
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 口頭発表(一般)
  • Nontargeted comprehensive analysis for persistent organohalogen compounds in finless porpoises from Seto Inland Sea.  [通常講演]
    野見山 桂
    19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO19) 2017年06月 口頭発表(一般)
  • 野生生物に残留するハロゲン化フェノール類の蓄積特性と毒性影響評価  [招待講演]
    野見山 桂
    第26回環境化学討論会 2017年06月 公開講演,セミナー,チュートリアル,講習,講義等
  • 医薬品類およびパーソナルケア製品由来物質(PPCPs)によるアジア途上国の水環境汚染の実態解明と魚類への移行/残留性評価  [通常講演]
    野崎一茶, 田上瑠美, 野見山 桂, Annamalai,A, Bulbule,K. A, Parthasarathy, P.・Viet, P. H, Tuyen,L. H, Sudaryanto, A, 磯部友彦, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • 魚類におけるヒト用医薬品類の取り込みと代謝および情動関連行動との関連解析〜オピオイド系鎮痛剤と抗うつ剤を例として〜  [通常講演]
    田上瑠美, Margiotta-Casaluci, L, Sumpter,J, 野見山 桂, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • 血清中メタボローム解析によるイエネコのPCBs毒性影響評価  [通常講演]
    野見山 桂, 西川博之, 水川葉月, 髙口倖暉, Sainnoxoi, T, 横山 望, 市居 修, 滝口満喜, 石塚 真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)の生体内変化  [通常講演]
    田中啓介, 野見山 桂, 水川葉月, 髙口倖暉, 田上瑠美, Saengtienchai, A, Sainnoxoi, T, 横山 望, 市居 修, 滝口満喜, 中山翔太, 池中良徳, 石塚 真由美, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • イエネコのin vivo 長期曝露試験:デカブロモジフェニルエーテル(BDE209)は甲状腺機能亢進症を引き起こすのか?  [通常講演]
    水川葉月, 野見山 桂, 池中良徳, 中山翔太, Saengtienchai, A, Sainnoxoi, T, 横山 望, 笹岡一慶, 市居 修, Khidkhan, K, 高居名菜, 滝口満喜, 田辺信介, 石塚 真由美
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • PCBs 曝露がイヌ・ネコの甲状腺ホルモン恒常性へ及ぼす影響  [通常講演]
    髙口倖暉, 野見山 桂, 西川博之, 水川葉月, 田上瑠美, 芳之内 結加, 横山 望, 市居 修, 滝口満喜, 中山翔太, 池中良徳, 石塚 真由美, 岩田久人, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • ポリ塩化ビフェニル(PCBs)暴露によるネコ甲状腺機能への影響評価~甲状腺ホルモンの脱ヨード化と代謝能の解析~  [通常講演]
    水川葉月, 高居名菜, 横山 望, 市居 修, 滝口満喜, 野見山 桂, 高口倖暉, 西川博之, 池中良徳, 中山翔太, Sainnoxoi, T, 田辺信介, 石塚 真由美
    第26回環境化学討論会 2017年06月 口頭発表(一般)
  • In vitro and in silico assessment of cytochrome P450-dependent biotransformation capacity of PCBs in the Caniformia; the Baikal seal vs the beagle dog.  [通常講演]
    Iwata, H, Yoo, J, Tamura, S, Hirano, M, Nomiyama, K, Tanabe, S
    SETAC North America 37th Annual Meeting/7th SETAC World Congress 2016年11月 ポスター発表 Orlando, FL, USA
  • Cross omics analysis for PCBs toxicity in the dog brain - Effect on mitochondrial functions  [通常講演]
    Takaguchi, K, Nomiyama, K, Yoo, J, Mizukawa, H, Iwata, H, Kunisue, T, Tanabe, S
    SETAC North America 37th Annual Meeting/7th SETAC World Congress 2016年11月 ポスター発表 Orlando, FL, USA
  • Characteristic for biotransformation of polychrolinated biphenyls in cats.  [通常講演]
    Mizukawa, H, Maehara, M, Yokoyama, N, Ichii, O, Takiguchi, M, Nomiyama, K, Nishikawa, H, Ikenaka, Y, Nakayama, S. M, Takaguchi, K, Tanabe, S, Ishizuka, M
    SETAC North America 37th Annual Meeting/7th SETAC World Congress 2016年11月 ポスター発表 Orlando, FL, USA
  • Accumulation features of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis.  [通常講演]
    Nomiyama, K, Yamamoto, Y, Mizukawa, H, Eguchi, A, Nakayama, S. M, Ikenaka, Y, Ishizuka, M, Kunisue, T, Tanabe, S
    SETAC North America 37th Annual Meeting/7th SETAC World Congress 2016年11月 ポスター発表 Orlando, FL, USA
  • A sensitive and reliable analytical method for free thyroid hormones in animal serum using ultrafiltration in combination with UHPLC-MS/MS.  [通常講演]
    Tanoue, R, Kume, I, Yamamoto, Y, Nomiyama, K, Tanabe, S, Kunisue, T
    SETAC North America 37th Annual Meeting/7th SETAC World Congress 2016年11月 口頭発表(一般) Orlando, FL, USA
  • Accumulation features of PCBs and their metabolites in American alligator.  [通常講演]
    Nomiyama, K, Nishikawa, H, Lowers, R, Guillette, L. J, Tanabe, S
    The 3rd Biennial Symposium 2016年11月 口頭発表(一般) Kennedy Space Center FL, USA NASA
  • Quantitative structure-relationships (QSAR) models for prediction of gas chromatography retention of methoxylated polychlorinated biphenyl using regularized multiple linear regressions  [通常講演]
    Eguchi, A, Nomiyama, K, Takaguchi, K, Mori, C
    The 9th International PCB Workshop 2016年10月 口頭発表(一般) Kobe, Japan
  • Hepatic transcriptome analysis to assess the effects of polychlorinated biphenyls on beagle dogs.  [通常講演]
    Tamura, S, Agusa, T, Hirano, M, Eguchi, A, Nomiyama, K, Tanabe, S, Kim, E, Iwata, H
    The 9th International PCB Workshop 2016年10月 ポスター発表 Kobe, Japan
  • Risk assessment of disruption of estrogen receptor signaling pathway by OH-PCBs in Baikal seals: application of in vitro and in silico approaches.  [通常講演]
    Yoshinouchi, Y, Hirano, M, Nomiyama, K, Tanabe, S, Kim, E, Iwata, H
    The 9th International PCB Workshop 2016年10月 ポスター発表 Kobe, Japan
  • Disruption of mitochondrial functions by PCB exposure in the dog brain: Toxicological assessment using cross omics.  [通常講演]
    Takaguchi, K, Nomiyama, K, Eguchi, A, Claret, L. M, Kusaki, M, Yoo, J, Mizukawa, H, Iwata, H, Kunisue, T, Tanabe, S
    The 9th International PCB Workshop 2016年10月 ポスター発表 Kobe, Japan
  • In vivo analysis of PCB metabolic capacities and effects on the thyroid hormone in cats.  [招待講演]
    Mizukawa, H, Maehara, M, Yokoyama, N, Ichii, O, Takiguchi, M, Nomiyama, K, Nishikawa, H, Takakuchi, K, Saengtienchai, A, Sainnoxoi, T, Ikenaka, Y, Nakayama, S. M. M, Tanabe, S, Ishizuka, M
    The 9th International PCB Workshop 2016年10月 口頭発表(一般) Kobe, Japan
  • Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis.  [通常講演]
    Nomiyama, K, Yamamoto, Y, Mizukawa, H, Takaguchi, K, Takiguchi, M, Eguchi, A, Nakayama, S, Ikenaka, Y, Ishizuka, M, Kunisue, T, Tanabe, S
    36th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2016) 2016年08月 口頭発表(一般) Firenze, Italy
  • Structural preference of OH-PCBs to activate the Baikal seal estrogen receptors.  [通常講演]
    Yoshinouchi, Y, Hirano, M, Nomiyama, K, Tanabe, S, Kim, E. Y, Iwata, H
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Assessment of metabolomics potential of polychlorinated biphenyl congeners in the beagle dog liver by in vitro and in silico approaches.  [通常講演]
    Yoo, J, Hirano, M, Nomiyama, K, Kim, E. Y, Tanabe, S, Iwata, H
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Proteomics analysis for PCBs toxicity in the dog brain.  [通常講演]
    Takaguchi, K, Nomiyama, K, Claret, L. M, Kusaki, M, Yoo, J, Mizukawa, H, Iwata, H, Kunisue, T, Tanabe, S
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Influence of lipid compositions on the brain distribution of PCBs and OH-PCBs in striped dolphins.  [通常講演]
    Ochiai, M, Nomiyama, K, Isobe, T, Yamada, T. K, Tajima, Y, Amano, M, Iwata, H, Tanabe, S
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Toxicological assessment of PCBs and hydroxyl-metabolites (OH-PCBs) in the brain of dogs using metabolomics approach.  [通常講演]
    Nomiyama,K, Eguchi, A, Mizukawa, H, Yoo, J, Takaguchi, K, Yamamoto, Y, Oshihoi, T, Iwata, H, Kunisue, T, Tanabe, S
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Elucidation of accumulated status of bioaccumulated organohalogen compounds in the finless porpoise from Seto Inland Sea  [通常講演]
    Motogi, K, Nomiyama, K, Goto, A, Ochiai, M, Tajima, Y, Yamada, T. K, Isobe, T, Kunisue, T, Tanabe, S
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Uptake and tissue distribution of pharmaceuticals and personal care products in wild fish from treated-waste water impacted streams.  [通常講演]
    Tanoue, R, Nomiyama, K, Nakamura, H, Kim, J. W, Isobe, T, Shinohara, R, Kunisue, T, Tanabe, S
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Halogenated flame retardants and polychlorinated biphenyls in wild avian species from Japan.  [通常講演]
    Fumoto, M, Tue, N. M, Goto, A, Nakatsu, S, Nomiyama, K, Tanabe, S, Kunisue, T
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Matsuyama, Japan
  • Biotransformation potencies of polychlorinated biphenyls in cats.  [通常講演]
    Mizukawa, H, Maehara, M, Yokoyama, N, Ichii, O, Takiguchi, M, Nomiyama, K, Nakayama, S. M. M, Tanabe, S, Ishizuka, M
    Joint Seminar on Environmental Chemistry and Toxicology ? Expand and Deepen the Knowledge of Environmental Research ? 2016年03月 Sapporo, Japan
  • Proteomic analysis for PCBs toxicity in the dog brain.  [通常講演]
    Takaguchi, K, Nomiyama, K, Claret, L. M, Kusaki, M, Yoo, J, Mizukawa, H, Iwata, H, Kunisue, T, Tanabe, S
    Joint Seminar on Environmental Chemistry and Toxicology ? Expand and Deepen the Knowledge of Environmental Research ? 2016年03月 ポスター発表 Sapporo, Japan
  • Toxicological assessment of PCBs and OH-PCBs in cat using metabolomics approach.  [通常講演]
    Nishikawa, H, Nomiyama, K, Eguchi, A, Mizukawa, H, Ishizuka, M, Ikenaka, Y, Nakayama, S. M. M, Tanabe, S
    Joint Seminar on Environmental Chemistry and Toxicology ? Expand and Deepen the Knowledge of Environmental Research ? 2016年03月 ポスター発表 Sapporo, Japan
  • Toxicological assessment of PCBs and OH-PCBs in the brain of dogs using metabolic approach.  [招待講演]
    Nomiyama, K, Takaguchi, K, Eguchi, A, Mizukawa, H, Yoo, J, Tamaru, S, Oshihoi, T, Iwata, H, Kunisue, T, Tanabe, S
    The 9th International PCB Workshop 2016年 口頭発表(一般) Kobe, Japan
  • Metabolic capacities of polychlorinated biphenyls (PCBs) in cats and dogs.  [通常講演]
    Mizukawa, H, Nomiyama, K, Tanabe, S, Maehara, M, Yokoyama, N, Ichii, O, Takiguchi, M, Ikenaka, Y, Nakayama, S. M. M, Ishizuka, M
    8th International Toxicology Symposium in Africa 2016年 口頭発表(一般) Giza, Egypt
  • Uptake and tissue distribution of pharmaceuticals and personal care products in wild fish inhabiting treated-wastewater impacted streams.  [招待講演]
    Tanoue, R, Nomiyama, K, Kim J. W, Isobe, T, Kunisue, T, Tanabe, S
    6th Joint Forum of Environmental Sciences 2016年 口頭発表(一般) National University, Gwangju, Korea
  • PCBsの in vivo 曝露によるビーグル犬の肝トランスクリプトームとメタボロームへの影響  [通常講演]
    田村沙弥, 阿草哲郎, 平野将司, 江口哲史, 野見山 桂, 田辺信介, 金 恩英, 岩田久人
    環境ホルモン学会第19回研究発表会 2016年 ポスター発表 つくば市
  • UGT2Bサブファミリーに着目した食肉目におけるグルクロン酸抱合能の動物種差解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, 坂本健太郎, 和田昭彦, 服部 薫, 三谷曜子, 田辺信介, 野見山 桂, 石塚真由美
    平成28年度内外環境応答・代謝酵素研究会 2016年 口頭発表(一般) 静岡市
  • 野生動物及び実験動物におけるグルクロン酸抱合酵素の動物種差解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, Saengtienchai, A, Beyene, Y, 坂本健太郎, 和田昭彦, 服部 薫, 三谷曜子, 田辺信介, 野見山 桂, 石塚真由美
    第159回日本獣医学会学術集会 2016年 口頭発表(一般) 藤沢市
  • アジア途上国を対象とした生活関連化学物質による魚類汚染  [通常講演]
    野見山 桂, 田上瑠美, 野崎一茶, 藤井定彦, 田辺信介, 国末達也
    第19回日本水環境学会シンポジウム, 2016年 口頭発表(一般) 秋田市
  • 下水処理水に残留する生活関連化学物質の魚への取り込みと排泄〜脳移行と残留性の解析〜  [通常講演]
    田上瑠美, 仲山 慶, 野見山 桂, 田辺信介, 国末達也
    第22回日本環境毒性学会研究発表会 2016年 口頭発表(一般) 松山市
  • クロスオミックスによるイヌ脳のPCBs毒性評価  [通常講演]
    高口倖暉, 野見山 桂, Claret, L. M, Jean, Y, 水川葉月, 岩田久人, 国末達也, 田辺信介
    日本プロテオーム学会 2016年 ポスター発表 東京
  • 茨城県に集団座礁したカズハゴンドウ(Peponocephala electra)に残留するPCBs, OH-PCBs の脳内負荷量と母子間移行  [通常講演]
    落合真理, 野見山 桂, 磯部友彦, 山田 格, 田島 木綿子, 岩田久人, 国末達也, 田辺信介
    日本セトロジー研究会第27回 2016年 口頭発表(一般) 長岡市
  • メタボロミクスアプローチを用いたイヌ脳へのOH-PCBs 毒性機序の解明  [通常講演]
    野見山 桂, 高口倖暉, 江口哲史, 水川葉月, Jean, Y, 田村沙弥, 忍穂井 智子, 岩田久人, 国末達也, 田辺信介
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • 野生鳥類におけるPOPs および代替難燃剤の汚染実態と蓄積特性  [通常講演]
    麓 光央, 後藤哲智, Tue, N. M, 中津 賞, 野見山 桂, 田辺信介, 国末達也
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • 日本近海沿岸に漂着した鯨類に残留する生物蓄積性有機ハロゲン化合物の網羅分析  [通常講演]
    元木一貴, 野見山 桂, 後藤哲智, 落合真理, 江頭佳奈, 田島 木綿子, 山田 格, 磯部友彦, 国末達也, 田辺信介
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • クロスオミックスによるイヌ脳へのPCBs 毒性評価:ミトコンドリア機能へ及ぼす影響解析  [通常講演]
    高口倖暉, 野見山 桂, Claret, L. M, 草木桃子, Jean, Y, 水川葉月, 岩田久人, 国末達也, 田辺信介
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • 有機ハロゲン化合物によるコンパニオンアニマルの汚染実態と甲状腺ホルモンへの影響評価  [通常講演]
    野見山 桂, 山本恭央, 水川葉月, 江口哲史, 滝口満喜, 中山翔太, 池中良徳, 石塚 真由美, 国末 達也, 田辺信介
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • ネコにおけるポリ塩化ビフェニル(PCBs)の異物代謝機構解明と甲状腺ホルモンへの影響評価  [通常講演]
    水川葉月, 前原美咲, 横山 望, 市居 修, 滝口満喜, 野見山 桂, 西川博之, 池中良徳, 中山翔太, 高口倖暉, 田辺信介, 石塚 真由美
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • 水酸化PCBs によるバイカルアザラシ エストロゲン受容体転写活性化能とその分子機構  [通常講演]
    芳之内 結加, 平野将司, 野見山 桂, 田辺信介, 金 恩英, 岩田久人
    第25回環境化学討論会 2016年 口頭発表(一般) 新潟
  • Impact of anthropogenic and naturally occurring brominated compounds in veterinary field.  [通常講演]
    Mizukawa, H, Yokoyama, N, Ichii, O, Takiguchi, M, Ikenaka, Y, Nakayama, S. M. M, Nomiyama, K, Maehara, M, Tanabe, S, Ishizuka, M
    Leading Symposium on Chemical Hazard and Adaptability in Animals - The Current Challenge of Young Researchers 2015年12月 口頭発表(一般) Sapporo, Japan
  • Toxicological assessment of polychlorinated biphenyls and their metabolites in the Baikal seal (Pusa sibirica).  [通常講演]
    Nomiyama, K, Hirakawa, S, Kanbara, C, Imaeda, D, Yoo, J, Kunisue, T, Kim, E. Y, Iwata, H, Tanabe, S
    Leading Symposium on Chemical Hazard and Adaptability in Animals - The Current Challenge of Young Researchers 2015年12月 口頭発表(一般) Sapporo, Japan
  • Lipid composition in the brain compartments of striped dolphins (Stenella coeruleoalba) in relation to the accumulation of environmental pollutants (PCBs and OH-PCBs)  [通常講演]
    Ochiai, M, Nomiyama, K, Isobe, T, Yamada, T, Tajima, Y, Iwata, H, Amano, M, Tanabe, S
    21st Biennial Society for Marine Mammalogy Conference on the Biology of Marine Mammals 2015年12月 口頭発表(一般) San Francisco, California, USA
  • Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats: Effects on thyroid hormones homeostasis.  [通常講演]
    Yamamoto, Y, Nomiyama, K, Mizukawa, H, Takiguchi, M, Ishizuka, M, Kunisue, T, Tanabe, S
    Society of Environmental Toxicology and Chemistry (SETAC) North America 36th Annual Meeting 2015年11月 ポスター発表 Salt Lake City, Utah, USA
  • Metabolism and biotransformation of organohalogen compounds in the liver microsomes of cats and dogs.  [通常講演]
    Mizukawa, H, Nomiyama, K, Yokoyama, N, Ichii, O, Takiguchi, M, Ikenaka, Y, Nakayama, S. M, Maehara, M, Tanabe, S, Ishizuka, M
    Society of Environmental Toxicology and Chemistry (SETAC) North America 36th Annual Meeting 2015年11月 口頭発表(一般) Salt Lake City, Utah, USA
  • Toxicological assessment of polychlorinated biphenyls (PCBs) and hydroxylated PCBs (OH-PCBs) in the brain of dogs using metabolomics approach.  [通常講演]
    Nomiyama, K, Eguchi, A, Mizukawa, H, Yoo, J, Takaguchi, K, Yamamoto, Y, Oshihoi, T, Iwata, H, Kunisue, T, Tanabe, S
    Society of Environmental Toxicology and Chemistry (SETAC) North America 36th Annual Meeting 2015年11月 ポスター発表 Salt Lake City, Utah, USA
  • Omics approach of proteomic and metabolomic analysis for PCBs toxicity in the dog brain.  [通常講演]
    Takaguchi, K, Nomiyama, K, Lauan, C. M, Yoo, J, Mizukawa, H, Iwata, H, Kunisue. T, Tanabe, S
    Society of Environmental Toxicology and Chemistry (SETAC) North America 36th Annual Meeting 2015年11月 ポスター発表 Salt Lake City, Utah, USA
  • Persistent environmental pollutants in Asian coastal waters ? contamination of dolphin brains.  [通常講演]
    Ochiai, M, Nomiyama, K, Isobe, T, Yamada, T. K, Tajima, Y, Makara, M, Amano, M, Matsuishi, T, Iwata, H, Tanabe, S
    Asian Society of Conservation Medicine Meeting, Myanmar 2015 2015年10月 口頭発表(一般) Yangon, Myanmar
  • ペット動物における有機ハロゲン化合物および水酸化代謝物の曝露実態と甲状腺ホルモン恒常性へ及ぼす影響  [通常講演]
    山本恭央, 野見山 桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺 信介
    環境ホルモン学会第18回研究発表会 2015年 口頭発表(一般) 下野市
  • イヌ脳中 PCBs, OH-PCBs の毒性影響評価:メタボロミクスアプローチ  [通常講演]
    野見山 桂, 江口哲史, 水川葉, Jean, Y, 高口倖暉, 山本泰央, 国末達也, 岩田久人, 田辺信介
    環境ホルモン学会第18回研究発表会 2015年 ポスター発表 下野市
  • トランスクリプトーム解析による PCBs を曝露したイヌへの影響評価  [通常講演]
    田村沙弥, 劉 珍, 水川葉月, 阿草哲郎, 平野将司, 江口哲史, 野見山 桂, 田辺信介, 金 恩英, 岩田久人
    環境ホルモン学会第18回研究発表会 2015年 ポスター発表 下野市
  • イヌ脳に及ぼす PCBs の影響:プロテオーム解析による評価  [通常講演]
    高口倖暉, 野見山 桂, Claret, L. M, Jean, Y, 水川葉月, 岩田久人, 国末達也, 田辺信介
    環境ホルモン学会第18回研究発表会 2015年 下野市
  • 鹿児島県南さつま市にマスストランディングしたスジイルカ (Stenella coeruleoalba) の脳内脂質組成とハロゲン化代謝物濃度  [通常講演]
    落合真理, 野見山 桂, 磯部友彦, 山田 格, 田島木綿子, 天野雅男, 田辺信介
    日本セトロジー研究会第26回 2015年 口頭発表(一般) 秋田
  • スナメリ体内に残留する有機ハロゲン化合物の網羅的スクリーニング ?GCxGC/TOF-MSによる検証?,  [通常講演]
    元木一貴, 野見山 桂, 後藤哲智, 落合真理, 田島木綿子, 山田 格, 磯部友彦, 国末達也, 田辺信介
    日本セトロジー研究会第26回 2015年 口頭発表(一般) 秋田
  • 生活関連化学物質による魚類汚染: 体内移行残留性と組織分布の解明およびそのリスク評価  [通常講演]
    田上瑠美, 野見山 桂, 篠原亮太, 田辺信介
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • 猛禽類における鉛中毒問題と骨における鉛分布の解明  [通常講演]
    石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 森田鮎子, 齊藤慶輔, 渡邊有希子, 神 和夫, 田辺信介, 野見山 桂, 林光武, 増田 泰, 坂本健太郎, 石塚真由美
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • 第II 相抱合反応の種差:食肉目におけるグルクロン酸抱合酵素の進化と遺伝子構造  [通常講演]
    池中良徳, 筧 麻友, 中山翔太, 川合佑典, 渡邊研右, 水川葉月, 野見山 桂, 田辺信介, 石塚真由美
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • 瀬戸内海のスナメリに残留する有機ハロゲン化合物の網羅分析  [通常講演]
    元木一貴, 野見山 桂, 後藤哲智, 落合真理, 国末達也, 田辺信介
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • PCBs 代謝物によるアメリカアリゲーターの汚染実態  [通常講演]
    西川博之, 野見山 桂, Lowers, R, Guillette, L. J. J, 田辺信介
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • ペットネコにおける有機ハロゲン化合物および水酸化代謝物の汚染実態と甲状腺ホルモン恒常性への影響  [通常講演]
    山本恭央, 野見山 桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺信介
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • ポリ塩化ビフェニル(PCBs)曝露によるイヌ異物代謝酵素シトクロムP450 発現への影響評価  [通常講演]
    田村沙弥, 劉 珍, 水川葉月, 阿草哲郎, 平野将司, 野見山 桂, 田辺信介, 金 恩英, 岩田久人
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • ポリ塩化ビフェニル(PCBs)を曝露したイヌの脳プロテオーム解析による毒性影響評価  [通常講演]
    高口倖暉, 野見山 桂, Claret, L. M, 草木桃子, Jean, Y, 水川葉月, 岩田久人, 国末達也, 田辺信介
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • メタボローム解析によるイヌ脳中PCBs, OH-PCBs の毒性影響評価  [通常講演]
    野見山桂, 江口哲史, 水川葉月, Jean, Y, 高口倖暉, 山本泰央, 忍穂井智子, 岩田久人, 国末達也, 田辺信介
    第24回環境化学討論会 2015年 口頭発表(一般) 札幌
  • 陸棲哺乳類の化学物質汚染:有機ハロゲン化合物および水酸化代謝物の暴露実態  [招待講演]
    野見山 桂
    四国自然史科学研究センター設立10周年記念イベント シンポジウム「四国の自然は、いま 2012」 2012年12月 シンポジウム・ワークショップパネル(指名)
  • Anthropogenic and naturally occurring halogenated phenolic compounds in the blood of cetaceans.  [招待講演]
    野見山 桂
    5th Bilateral Seminar Italy-Japan 2012年11月 口頭発表(招待・特別)

作品等

  • PPCPsによる水環境汚染に関する研究
    野見山 桂  2010年 - 現在
  • ペット動物の化学汚染に関する研究
    野見山 桂  2010年 - 現在
  • 僕が四国で生きものを撮るということ~写真展~
    野見山 桂  2017年05月 -2017年05月
  • 僕が四国で生きものを撮るということ~写真展~
    野見山 桂  2016年12月 -2016年12月

MISC

  • Species-Specific Differences in Extractable Organochlorine and Organobromine in High-Trophic-Level Mammals
    Mukai, K, Fujimori, T, Anh, H. Q, Fukutani, S, Kunisue, T, Nomiyama, K, Takahashi, S Dioxin 2019 - 39th International Symposium on Halogenated Persistent Organic Pollutants, Kyoto, Japan 研究発表ペーパー・要旨(国際会議) 2019年08月
  • 向井康太, 藤森崇, ANH Hoang Quoc, 福谷哲, 国末達也, 野見山桂, 高橋真, 高橋真 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.P‐037 2019年06月
  • 水川葉月, NGYUEN Hoa Thanh, 岩田久人, 野見山桂, 池中良徳, 中山翔太, 横山望, 市居修, 滝口満喜, KHIDKHAN Kraisiri, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.1C‐08 2019年06月
  • 池中良徳, 池中良徳, 一瀬貴大, NIMAKO Collins, 市川剛, 野見山桂, 長谷川浩, 中山翔太, 星信彦, 平久美子, 石塚真由美 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.2B‐05 2019年06月
  • 田中啓介, 野見山桂, 水川葉月, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.1C‐09 2019年06月
  • 一瀬貴大, 池中良徳, 池中良徳, 三津橋嵩史, 中山翔太, SAENGTIENCHAI Aksorn, 野見山桂, 田上瑠美, 石塚真由美 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.P‐106 2019年06月
  • 環境多媒体中の抽出可能性塩素・臭素の中性子放射化分析
    向井康太, 藤森崇, Hoang Quoc Anh, 福谷哲, 国末達也, 野見山桂, 高橋真 放射化分析及び中性子を用いた地球化学研究-2- 研究発表ペーパー・要旨(全国大会,その他学術会議) 2019年01月
  • Characterization of Extractable Organohalogens (EOX) in Biological Samples
    Mukai, K, Fujimori, T, Anh, H. Q, Fukutani, S, Kunisue, T, Nomiyama, K, Takahashi, S 2nd Chemical Hazard Symposium, Ehime, Japan 研究発表ペーパー・要旨(国際会議) 2018年12月
  • 近藤 誉充, 池中 良徳, 中山 翔太, 水川 葉月, 三谷 曜子, 野見山 桂, 田辺 信介, 石塚 真由美 日本毒性学会学術年会 45 (0) P -60 2018年08月 
    <p>アザラシやネコ科を含む食肉目動物は生態系高次栄養段階に位置し、食物連鎖を介した生物濃縮により残留性の高い有機化合物が高濃度で生体内に蓄積している。化学物質代謝酵素はこれら化学物質の解毒を担う酵素である。その中でも特に第II相抱合酵素はCytochrome P450等の代謝を受けた化学物質をさらに代謝する酵素であり、発がん性物質を含む多くの化学物質がP450の代謝を受けた後に代謝的活性化を示すため、第II相酵素は特に解毒に重要な酵素である。しかし多くの食肉目動物で第II相抱合酵素の情報は皆無であり、特に基本的情報である遺伝的性状や酵素活性の種差の情報が欠如している。そこで本研究は主要な第II相抱合酵素であるグルクロン酸転移酵素(UGT)および硫酸転移酵素(SULT)の遺伝子性状およびin vitro酵素活性の解明を目的とした。遺伝的性状解析では遺伝子データベースやシークエンス解析による遺伝子情報から系統解析、および遺伝子コード領域の種差を解明した。In vitro活性解析ではネコ、イヌ、ラット、および鰭脚類(カスピカイアザラシ、ゼニガタアザラシ、トド、キタオットセイ)の肝ミクロソームおよびサイトゾルを用いて種々の分子種特異的な基質(Lorazepam:UGT2B分子種, Estradiol:SULT1E1等)に対する酵素活性を測定した。UGTに関して、系統解析の結果から食肉目で特に重要と推定される2B31分子種の存在が明らかとなった。また、食肉目の中でもイヌでは3つのUGT2B31を持つのに対して、ネコ科動物では2B分子種が存在せず、鰭脚類でも1つの分子種しか持たないことが明らかとなった。さらにin vitro活性もイヌと比較してネコ及びアザラシ科で非常に低い活性が確認された。SULTに関しては、鰭脚類でエストロゲン代謝に重要なSULT1E1分子種が遺伝的に欠損しており、in vitro活性も低いことが解明された。これらの結果から食肉目動物の中でもとくに鰭脚類やネコ科動物では第II相抱合酵素による解毒能が弱く、種々の化学物質に対して感受性が強い可能性が示唆された。</p>
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美 日本野生動物医学会大会・講演要旨集 24th 151 2018年08月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 野見山桂, 田辺信介, 石塚真由美 Journal of Toxicological Sciences 43 (Supplement) S238 2018年06月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐09(PA‐18) 2018年05月
  • 島崎真琴, 野見山桂, 水川葉月, SAENGTIENCHAI Aksorn, NGAMCHIRTTAKUL Araya, PENCHAREE Disdanai, 星野光希, 山本恭央, 高口倖暉, 石塚真由美, 池中良徳, 中山翔太, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐03(PA‐14) 2018年05月
  • 野見山桂, 江口哲史, 西川博之, 水川葉月, 山本恭央, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 田辺信介 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐11 2018年05月
  • 水川葉月, KHIDKHAN Kraisiri, 高口倖暉, 横山望, 市居修, 滝口満喜, 笹岡一慶, 池中良徳, 池中良徳, 中山翔太, TSEND‐AYUSH Sainnoxoi, 野見山桂, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐10 2018年05月
  • 田中啓介, 野見山桂, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.1A‐03(PA‐13) 2018年05月
  • 化学物質と甲状腺機能への影響
    野見山 桂 環境ホルモン学会News Letter 記事・総説・解説・論説等(学術雑誌) 21 (2) 2018年 [招待有り]
  • 高口 倖暉, 中山 翔太, 池中 良徳, 石塚 真由美, 岩田 久人, 国末 達也, 田辺 信介, 野見山 桂, 西川 博之, 水川 葉月, 田上 瑠美, 草木 桃子, 横山 望, 市居 修, 滝口 満喜 日本毒性学会学術年会 45 (0) P -72 2018年 

    近年、ペット動物に対するポリ塩化ビフェニル(PCBs)および水酸化代謝物(OH-PCBs)の曝露による甲状腺ホルモン(THs)の恒常性への影響が注目されている。本研究ではPCBs曝露がペット動物のTHs恒常性に及ぼす影響を明らかにするため、イヌ・ネコのPCBs投与試験を実施し、PCBs曝露に伴う血清中THs濃度の変化を解析した。

    PCBs投与後から5日間、継続的に血清を採取し、THs濃度の変化を解析した結果、ネコ血清中THs濃度は、総THs、遊離型THsともに対照群と投与群の間に有意な変化は認められなかった。一方、イヌ血清中総THs濃度は投与群において、総L-サイロキシン(T4)と総3,5,3'-トリヨード-L-サイロニン(T3)の減少傾向がみられ、総PCBs濃度は総T4、総T3濃度と有意な負の相関を示した(p < 0.01)。一方、遊離型T4、遊離型T3濃度では、対照群と投与群の間に経時的な変化は認められなかったが、曝露後48、96時間目で有意に増加した(p < 0.05)。加えて、遊離型T4濃度は総OH-PCBs濃度と正の相関を示し(p < 0.01)、異性体別ではT4様構造の高塩素化OH-PCBsで同様の傾向が認められた。上記の結果および先行研究から、PCBs曝露によるイヌ血清中THsへの影響を推察した。PCBsがT4様構造のOH-PCBsへ代謝されてTHs輸送タンパクに競合結合し、結合できない遊離型T4が血中に増加することで、THsの臓器・組織への取り込み量が増加したと予測される。その結果、イヌ肝臓中のTHs濃度は増加し、PCBs曝露により肝臓中のAhR・CARが過剰に誘導されることで、UGTおよびSULTが誘導され、THsの抱合化を促進することで体外排泄量が増加し、血清中総T4、T3が減少したものと推察された。

  • イヌ・アザラシ・マウスのエストロゲン受容体転写活性化能の種差を規定する分子機序
    芳之内 結加, 奥田 博貴, 平野 将司, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 環境ホルモン学会研究発表会要旨集 20回 41 -41 2017年12月
  • 水川葉月, 高居名菜, 野見山桂, 高口倖暉, 横山望, 市居修, 滝口満喜, 池中良徳, 池中良徳, 中山翔太, 石塚真由美 日本内分泌かく乱化学物質学会研究発表会要旨集 20th 78 -78 2017年12月
  • 水川葉月, 高居名菜, 横山望, 市居修, 滝口満喜, 野見山桂, 高口倖暉, 西川博之, 池中良徳, 池中良徳, 中山翔太, SAINNOXOI Tsend‐ayush, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐01 2017年06月
  • 高口倖暉, 野見山桂, 西川博之, 水川葉月, 田上瑠美, 芳之内結加, 横山望, 市居修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 岩田久人, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐02 2017年06月
  • 水川葉月, 野見山桂, 池中良徳, 池中良徳, 中山翔太, SAENGTIENCHAI Aksorn, SAINNOXOI Tsendayush, 横山望, 笹岡一慶, 市居修, KHIDKHAN Kraisiri, 高居名菜, 滝口満喜, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐03 2017年06月
  • 田中啓介, 野見山桂, 水川葉月, 高口倖暉, 田上瑠美, SAENGTIENCHAI Aksorn, SAINNOXOI Tsend‐ayush, 横山望, 市居修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐04 2017年06月
  • 野見山桂, 西川博之, 水川葉月, 高口倖暉, SAINNOXOI Tsend‐ayush, 横山望, 市居修, 滝口満喜, 石塚真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐05 2017年06月
  • 水酸化PCBsによるアザラシ・イヌエストロゲン受容体転写活性化能の評価
    芳之内 結加, 奥田 博貴, 平野 将司, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 The Journal of Toxicological Sciences 42 (Suppl.) S236 -S236 2017年06月
  • 水川葉月, 野見山桂, 田辺信介, 池中良徳, 池中良徳, 中山翔太, 石塚真由美 Journal of Toxicological Sciences 42 (Supplement) S36 2017年06月
  • 哺乳類に残留する有機ハロゲン代謝物の脳移行とリスク評価の試み
    野見山 桂 環境ホルモン学会 News Letter 研究発表ペーパー・要旨(全国大会,その他学術会議) 20 (1) 2 2017年 [招待有り]
  • 水川 葉月, 中山 翔太, Tsend-ayush SAINNOXOI, 田辺 信介, 石塚 真由美, 高居 名菜, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 高口 倖暉, 西川 博之, 池中 良徳 日本毒性学会学術年会 44 (0) P -55 2017年 
    有機ハロゲン化合物の一種であるポリ塩化ビフェニル(PCBs)は甲状腺ホルモン(THs)の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、in vivo投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度(総T4、総T3、遊離T4、遊離T3、リバースT3)を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子(甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1)の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素(UGT1A1)の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。
    本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。
  • 水川 葉月, 中山 翔太, Tsend-ayush SAINNOXOI, 田辺 信介, 石塚 真由美, 高居 名菜, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 高口 倖暉, 西川 博之, 池中 良徳 日本毒性学会学術年会 44 (0) P -55 2017年 
    有機ハロゲン化合物の一種であるポリ塩化ビフェニル(PCBs)は甲状腺ホルモン(THs)の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、in vivo投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度(総T4、総T3、遊離T4、遊離T3、リバースT3)を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子(甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1)の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素(UGT1A1)の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。
    本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。
  • 田村沙弥, 阿草哲郎, 平野将司, 江口哲史, 野見山桂, 田辺信介, KIM Eun‐Young, 岩田久人 日本内分泌かく乱化学物質学会研究発表会要旨集 19th 80 2016年12月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, SAENGTIENCHAI Aksorn, BEYENE Yared, 坂本健太郎, 和田昭彦, 服部薫, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美 日本獣医学会学術集会講演要旨集 159th 493 2016年08月
  • 水川葉月, 前原美咲, 横山望, 市居修, 滝口満喜, 野見山桂, 西川博之, 池中良徳, 中山翔太, 高口倖暉, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐13 2016年06月
  • 西川博之, 野見山桂, 水川葉月, 横山望, 市居修, 滝口満喜, 石塚真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐12 2016年06月
  • 野見山桂, 山本恭央, 水川葉月, 江口哲史, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐14 2016年06月
  • 野見山桂, 高口倖暉, 江口哲史, 水川葉月, YOO Jean, 田村沙弥, 忍穂井智子, 岩田久人, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.P‐030 2016年06月
  • 江口哲史, 野見山桂, 高口倖暉, 森千里, 森千里 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.P‐005 2016年06月
  • 水川 葉月, 高口 倖暉, 田辺 信介, 石塚 真由美, 前原 美咲, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 西川 博之, 池中 良徳, 中山 翔太 日本毒性学会学術年会 43 (0) P -6 2016年 
    ポリ塩化ビフェニル(PCBs)の水酸化代謝物であるOH-PCBsは、肝臓内で薬物代謝酵素より生成され、その後体外へ排泄される。しかしながら、一部の水酸化代謝物は甲状腺ホルモン(TH)と類似の構造をもつため、THの恒常性を撹乱することが危惧されている。これまでに、多様な陸棲哺乳類の血中OH-PCBsを分析したところ、種間でOH-PCBsの組成に差異が認められ、中でも、ネコのOH-PCBs残留パターンは他種と大きく異なることから、本種は特異な代謝機能を有することが示唆された。しかし、ネコの異物代謝能の研究は僅かであり、化学物質暴露による毒性影響も不明な点が多い。本研究では、ネコにおけるPCBs in vivo暴露試験を実施し、体内動態および代謝に関与する酵素活性や遺伝子を解析するとともに、化学物質の暴露評価に繋がる基盤的情報の収集を目的とした。
    コーン油に溶解した12異性体のPCBsを腹腔内投与し、経時採血した血清中PCBsおよびOH-PCBs濃度について同条件で実施したイヌのin vivo試験と比較した結果、異性体の残留パターンや体内動態にイヌとネコで種差が観察された。とくにネコでは低塩素化体の残留が顕著であった。また、代謝酵素活性および遺伝子解析の結果、PCBs暴露によりEROD、MROD、PROD活性は上昇するものの、第2相抱合酵素(UGTやSULT)活性は変化せず、PCBs暴露による抱合酵素活性への影響もみられなかった。また、CYP1A1およびCYP1A2遺伝子の発現量の上昇も認められた。
    本研究により、ネコのPCBs吸収・代謝・排泄能はイヌと異なることが示唆され、とくに低塩素化OH-PCBsの毒性リスクは高いことが予想された。低塩素化OH-PCBsは血中でTH輸送タンパクとの競合結合や、THの硫酸抱合排泄の阻害、TH起因性遺伝子の転写抑制などが報告されており、ネコの甲状腺機能障害が懸念される。
  • イヌ脳に及ぼすPCBsの影響 プロテオーム解析による評価
    高口 倖暉, 野見山 桂, Lauan Maria Claret, Yoo Jean, 水川 葉月, 岩田 久人, 国末 達也, 田辺 信介 環境ホルモン学会研究発表会要旨集 18回 106 -106 2015年12月
  • トランスクリプトーム解析によるPCBsを曝露したイヌへの影響評価
    田村 沙弥, 劉 珍, 水川 葉月, 阿草 哲郎, 平野 将司, 江口 哲史, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 環境ホルモン学会研究発表会要旨集 18回 116 -116 2015年12月
  • イヌ脳中PCBs、OH-PCBsの毒性影響評価 メタボロミクスアプローチ
    野見山 桂, 江口 哲史, 水川 葉月, Yoo Jean, 高口 倖暉, 山本 泰央, 国末 達也, 岩田 久人, 田辺 信介 環境ホルモン学会研究発表会要旨集 18回 117 -117 2015年12月
  • OH-PCBsによるバイカルアザラシエストロゲン受容体α・β転写活性化能の評価
    芳之内 結加, 平野 将司, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 環境ホルモン学会研究発表会要旨集 18回 142 -142 2015年12月
  • 山本恭央, 野見山桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺信介 日本内分泌かく乱化学物質学会研究発表会要旨集 18th 124 2015年12月
  • 山本恭央, 野見山桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.1A‐20 2015年06月
  • 池中良徳, 筧麻友, 中山翔太, 川合佑典, 渡邊研右, 水川葉月, 野見山桂, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.3A‐06 2015年06月
  • 石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 森田鮎子, 齊藤慶輔, 渡邊有希子, 神和夫, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.3D‐05 2015年06月
  • 野見山桂, 江口哲史, 水川葉月, YOO Jean, 高口倖暉, 山本泰央, 忍穂井智子, 岩田久人, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.1A‐06 2015年06月
  • 野見山桂, 平川周作, 江口哲史, 今枝大輔, YOO Jean, 国末達也, 岩田久人, 田辺信介 日本内分泌かく乱化学物質学会研究発表会要旨集 17th 107 2014年12月
  • ポリ塩化ビフェニル(PCBs)曝露によるイヌ異物代謝酵素シトクロムP450への影響
    田村 沙弥, Yoo Jean, 水川 葉月, 阿草 哲郎, 平野 将司, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 環境ホルモン学会研究発表会要旨集 17回 106 -106 2014年12月
  • 石井千尋, 中山翔太, 池中良徳, 水川葉月, 中谷北斗, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 日本獣医学会学術集会講演要旨集 157th 533 2014年08月
  • 石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 J Toxicol Sci 39 (Supplement) S336 -188 2014年07月 
    【はじめに】北海道では、猛禽類において鉛弾摂取による鉛中毒が問題となっている。現在は鉛弾の使用規制が設けられているものの、未だに被害個体が発見されている。また、本州など他地域でも鉛中毒個体が発見されているが、北海道を除く地域における汚染実態調査は極僅かであり、鉛弾使用規制も不十分である。そこで、本研究では北海道、本州、四国において死亡した猛禽類の鉛濃度と鉛安定同位体比を測定し、鉛汚染状況と汚染源の解明を試みた。<br>【方法】試料は、1993年から2014年に北海道、本州、四国で死亡したイヌワシ、トビ、オオワシ、オオタカ、フクロウ、オジロワシ、オオハヤブサ、クマタカ、サシバ、ハイタカ、ツミ、チョウゲンボウの計49個体の肝臓を採取し、誘導結合プラズマ質量分析計で鉛濃度と鉛安定同位体比(<sup>207</sup>Pb/<sup>206</sup>Pb、<sup>208</sup>Pb/<sup>206</sup>Pb)を分析した。<br>【結果と考察】本研究の結果、1999‐2014年に北海道で死亡した4羽のオオワシにおいて、鉛中毒が疑われる値(11.0-29.5 mg/kg、湿重量)が検出された。北海道では2004年に大型獣の狩猟における鉛弾使用が禁止されているが、2013、2014年に採取されたオオワシにおいても高濃度の鉛蓄積が検出され、このうち1羽からはレントゲン撮影と剖検で胃内に鉛弾の破片が認められた。また岩手県で死亡したイヌワシからも高濃度の鉛蓄積(2.0 mg/kg)が認められた。さらに鉛安定同位体比を分析した結果、高濃度の鉛蓄積個体の鉛安定同位体比の値が同程度であった。このうち1羽から鉛弾が検出されたことを考慮すると、これらの高濃度蓄積個体の汚染源は鉛ライフル弾であると考えられた。<br> 上記の結果から、猛禽類の鉛弾摂取による中毒が未だに問題となっていることが明らかとなった。従って、北海道以外の地域における調査、また日本全国における鉛弾流通の完全禁止など規制強化の検討が必要であると考えられた。
  • 筧麻友, 中山翔太, 水川葉月, 池中良徳, 渡邊研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 J Toxicol Sci 39 (Supplement) S281 -78 2014年07月 
    【目的】グルクロン酸抱合酵素(UGT)は、生体外異物代謝の第Ⅱ相抱合反応を担い、各動物の化学物質感受性決定に関与することが報告されている。食肉目ネコ亜目(Feliformia)では環境化学物質や薬物等の代謝に関与するUGT1A6の偽遺伝子化が報告されており、この偽遺伝子化に伴いアセトアミノフェン等の薬物の毒性作用が強いことが知られている。一方、食肉目に属する鰭脚類(Pinnipedia)では、環境化学物質の高濃度蓄積が報告されているが、感受性に関与するUGTについての研究はほとんど行われていない。そこで、鰭脚類を中心とした食肉目において、肝臓でのUGT活性の測定と系統解析を行った。<br>【方法】食肉目に属するネコ(<i>Felis catus</i>)、イヌ(<i>Canis familiaris</i>)、鰭脚類であるトド(<i>Eumetopias jubatus</i>)、キタオットセイ(<i>Callorhinus ursinus</i>)、カスピカイアザラシ(<i>Phoca caspica</i>)及び対照としてラット(<i>Rattus norvegicus</i>)の肝臓ミクロソームを作成し、1-ヒドロキシピレン(UGT1A6、UGT1A7、UGT1A9)、アセトアミノフェン(UGT1A1、UGT1A6、UGT1A9)、セロトニン(UGT1A6)を基質としてUGT活性を測定した。また、NCBIのデータベースからUGT1A領域の系統解析およびシンテニー解析を行った。<br>【結果及び考察】1-ヒドロキシピレン、アセトアミノフェン、セロトニンに対するUGT抱合活性を測定した結果、ラットに比べ食肉目では極めて低い活性を示した。また、系統解析及びシンテニー解析より、解析した全ての食肉目において、UGT1A分子種は特徴的な2遺伝子であるUSP40 とMROH2の間に保存されていることが明らかになった。さらに、食肉目は齧歯目に比べUGT1A領域が短く、UGT1A分子種数が少ないことが確認された。以上の結果から、鰭脚類を含めた食肉目はUGTによる異物代謝能が低く、環境化学物質に対する感受性が高い可能性が考えられた。
  • 筧麻友, 池中良徳, 中山翔太, 水川葉月, 渡邊研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 J Toxicol Sci 39 (Supplement) S223 -9 2014年07月 
    【目的】グルクロン酸抱合酵素(UGT)は、異物代謝の第Ⅱ相抱合反応を担い、各動物の化学物質感受性決定に関与することが報告されている。食肉目ネコ亜目(<i>Feliformia</i>)では環境化学物質や薬物等の代謝に関与するUGT1A6の偽遺伝子化が報告されており、この偽遺伝子化によりアセトアミノフェン等の薬物の毒性作用が強く表れることが知られている。一方、食肉目に属する鰭脚類(<i>Pennipedia</i>)では、PCB、DDTなどの残留性有機汚染物質の高濃度蓄積が報告されているが、感受性に関与するUGTについての研究がほとんど行われていない。そこで、鰭脚類を中心とした食肉目において、UGTの機能解析及び系統解析を行い、種差を明らかにすることを目的とした。<br>【方法】食肉目に属するネコ(<i>Felis catus</i>)、イヌ(<i>Canis familiaris</i>)、鰭脚類であるトド(<i>Eumetopias jubatus</i>)、キタオットセイ(<i>Callorhinus ursinus</i>)、カスピカイアザラシ(<i>Phoca caspica</i>)及び対照としてラット(<i>Rattus norvegicus</i>)の肝臓ミクロソームを調整した。この肝臓ミクロソームを用いて、1-ヒドロキシピレンを基質としてUGT活性を測定した。さらに食肉目UGT1A遺伝子について、他の哺乳類と比較し、系統解析を行った。<br>【結果及び考察】1-ヒドロキシピレンを基質として用いたところ、イヌはラットと同程度のUGT活性を示したが、鰭脚類3種およびネコのUGT活性はラットの3分の1以下だった。また、UGT1A遺伝子は一般に2-10程度のホモログが近接して染色体上に存在しているが、系統解析及びシンテニー解析より、食肉目は齧歯目に比べUGT1A領域が短くUGT1A分子種数が少ないことが確認された。さらに、トドおよびキタオットセイでは、UGT1A6のエクソン1領域に2塩基挿入によるストップコドンが生じ、偽遺伝子化していることが明らかになった。以上の結果より、鰭脚類は、UGTによる異物代謝能が低く、環境化学物質に対する感受性が高い可能性が考えられた。
  • 水川葉月, 池中良徳, 野見山桂, 田辺信介, 宇根有美, 伊藤真輝, 高江洲昇, 長野靖子, 中山翔太, 石塚真由美 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3B-14 2014年05月
  • 石井千尋, 池中良徳, 中山翔太, 水川葉月, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3D-03 2014年05月
  • 江口哲史, 野見山桂, 落合真理, 水川葉月, 長野靖子, 神原千佳, 中川勝博, 田中幸樹, 宮川治彦, 田辺信介 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3B-10 2014年05月
  • 筧麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 日本野生動物医学会大会・講演要旨集 20th 75 2014年
  • 筧麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 日本分子生物学会年会プログラム・要旨集(Web) 37th 3P-0853 (WEB ONLY) 2014年
  • 江口哲史, 野見山桂, TUE Nguyen Minh, TRANG Pham Thi Kim, VIET Pham Hung, 高橋真, 田辺信介 日本内分泌かく乱化学物質学会研究発表会要旨集 16th 163 2013年12月
  • 水川葉月, 野見山桂, 中津賞, 久保田彰, 石塚真由美, 岩田久人, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-2D-7-3 2013年07月
  • 野見山桂, 江口哲史, 神原千佳, 今枝大輔, 平川周作, KIM Eun‐Young, 岩田久人, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-2D-7-1 2013年07月
  • 江口哲史, 野見山桂, TUE Nguyen Minh, VIET Pham Hung, PARTHASARATHY, Peethambaram, BULBULE Kesav A, SUBRAMANIAN Annamalai, 高橋真, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.1PC-30 2013年07月
  • 染矢雅之, 江口哲史, 鈴木剛, 野見山桂, 後藤哲智, 高橋真, 滝上英孝, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-1A-6-3 2013年07月
  • 長野靖子, 野見山桂, 水川葉月, 江口哲史, 中津賞, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-2D-7-2 2013年07月
  • 江口哲史, 野見山桂, TUE Nguyen Minh, 国末達也, WU Qian, VIET Pham Hung, 高橋真, KANNAN Kurunthachalam, 田辺信介 環境化学討論会要旨集(CD-ROM) 21st ROMBUNNO.1C-01 2012年07月
  • 野見山桂, 神原千佳, 水川葉月, 江口哲史, 落合真理, 磯部友彦, 山田格, 田辺信介 環境化学討論会要旨集(CD-ROM) 21st ROMBUNNO.1C-04 2012年07月
  • 染矢雅之, 鈴木剛, 野見山桂, 江口哲史, 後藤哲智, 滝上英孝, 田辺信介 環境化学討論会要旨集(CD-ROM) 21st ROMBUNNO.P-041 2012年07月
  • 江口哲史, 野見山桂, 中川勝博, 田中幸樹, 宮川治彦, 田辺信介 環境化学討論会要旨集(CD-ROM) 21st ROMBUNNO.P-033 2012年07月
  • Transfer potency and accumulation features of halogenated phenolic compounds into the brain of finless porpoise (Neophocaena phocaenoides)
    Ochiai M, Nomiyama K, Isobe T, Yamada T.K, Tajima Y, Makara M, Amano M, Tanabe S Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 74 1471 -1474 2012年 [査読有り]
     
    Proceedings
  • Organohalogen Compounds and Their Metabolites in the Blood of Japanese Amberjack and Scalloped Hammerhead Shark from Japanese Coastal Waters.
    Nomiyama K, Uchiyama Y, Horiuchi S, Eguchi A, Kanbara C, Horai-Hirata S, Shinohara R, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 6 177 -185 2012年 [査読有り]
     
    Proceedings
  • Accumulation features of halogenated phenolic compounds in the blood of pinnipeds from Japanese coastal waters
    Nomiyama K, Kanbara C, Mizukawa H, Eguchi A, Ochiai M, Isobe T, Yamada T.K, Tanabe S Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 74 931 -934 2012年 [査読有り]
     
    Proceedings
  • Accumulation of Halogenated Phenolic Compounds in Small Toothed Whales
    Ochiai M, Nomiyama K, Isobe T, Yamada T.K, Tajima Y, Matsuishi T, Amano M, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 6 187 -192 2012年 [査読有り]
     
    Proceedings
  • Accumulation Features of Organohalogen Metabolites in the Blood of Japanese Terrestrial Mammals.
    Mizukawa H, Nomiyama K, Nakatsu S, Yachimori S, Hayashi T, Tashiro Y, Yamamoto M, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 6 203 -210 2012年 [査読有り]
     
    Proceedings
  • Contamination Status and Accumulation Features of Organohalogen Compounds in Raccoon Dog and Masked Palm Civet
    Yamamoto M, Isobe T, Hayashi T, Yachimori S, Nomiyama K, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 6 211 -219 2012年 [査読有り]
     
    Proceedings
  • Kei Nomiyama, Yukiko Uchiyama, Satoko Horiuchi, Akifumi Eguchi, Hazuki Mizukawa, Sawako Horai Hirata, Ryota Shinohara, Shinsuke Tanabe CHEMOSPHERE 85 (3) 315 -321 2011年10月 
    Information on accumulation of polychlorinated biphenyl metabolites (OH-PCBs) and hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in the blood of marine fish is limited. The present study, we determined the residue levels and patterns of PCBs, OH-PCBs, PBDEs, OH-PBDEs and methoxylated PBDEs (MeO-PBDEs) in the blood collected from scalloped hammerhead shark (Sphyrna lewini) and Japanese amberjack (Seriola quinqueradiata), species of predatory fish at Japanese coastal waters. The predominant homologues found in Japanese amberjacks were mono- and di-chlorinated OH-PCBs, and scalloped hammerhead sharks were octa-chlorinated OH-PCBs. The predominant OH-PCB isomers were lower-chlorinated OH-PCBs such as 6OH-CB2 and 2'OH-CB9 in Japanese amberjacks. This result suggests that exposure of Japanese amberjacks to lower-chlorinated OH-PCBs might be from the ambient aquatic environment. In scalloped hammerhead sharks, 4,4'diOH-CB202, 4OH-CB201 and 4OH-CB146 were the predominant isomers accounting for approximately 60% of the total OH-PCBs. The predominant MeO-PBDE isomers were 6MeO-BDE47 followed by 2'MeO-BDE68 in both species. As for OH-PBDE isomers, 6OH-BDE47 was predominant followed by 2'OH-BDE68 in Japanese amberjacks and scalloped hammerhead sharks. Residue levels of Sigma MeO-PBDEs and Sigma OH-PBDEs showed a significant positive correlation (p = 0.029). This result suggests that MeO-PBDEs and OH-PBDEs share a common source or a metabolic pathway in fishes. Characteristic differences found in the profiles of OH-PCBs and OH-PBDEs in Japanese amberjack and scalloped hammerhead shark show the need for further studies on the differences in exposure profiles, metabolic capacities and toxic effects in fish. (C) 2011 Elsevier Ltd. All rights reserved.
  • Sawako Horai Hirata, Daisuke Hayase, Akifumi Eguchi, Takaaki Itai, Kei Nomiyama, Tomohiko Isobe, Tetsuro Agusa, Toshiyuki Ishikawa, Michio Kumagai, Shinsuke Tanabe ENVIRONMENTAL POLLUTION 159 (10) 2789 -2796 2011年10月 
    The present study measured the concentrations of 25 elements (Li, Mg, V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Mo, Ag, Cd, In, Sn, Sb, Cs, Ba, Hg, Tl, Pb and Bi) in the whole body of Isaza which is an endemic fish species to Lake Biwa, Japan, and compared the values in the specimens from the mass mortality Isaza (MMI) and normal fresh Isaza (NFI). The mean levels of Mn and total As (T-As) were relatively higher in MMI than in NFI. In the T-As, highly toxic inorganic As was detected in MMI. Moreover we found Mn and As concentrations in surface sediment were extremely high and temporally increased. From all these results, we could infer that the dissolution of Mn and As from surface sediment of Lake Biwa might have been one of the cause for the mass mortality of Isaza. (C) 2011 Elsevier Ltd. All rights reserved.
  • 野見山桂, 江口哲史, 村田仁子, 水川葉月, 染矢雅之, 磯部友彦, 山田格, 田辺信介 環境化学討論会要旨集(CD-ROM) 20th ROMBUNNO.2B-06 2011年07月
  • 神原千佳, 野見山桂, 水川葉月, 江口哲史, 磯部友彦, 山田格, 高橋真, 田辺信介 環境化学討論会要旨集(CD-ROM) 20th ROMBUNNO.P-053 2011年07月
  • Halogenated Phenolic Compounds in the Blood of Japanese Amberjack and Scalloped Hammerhead Shark from Japanese Coastal Waters
    Nomiyama K, Uchiyama Y, Horiuchi S, Eguchi A, Hirata-Horai S, Shinohara R, Tanabe S Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 73 1199 -1202 2011年 [査読有り]
     
    Proceedings
  • Accumulation of Anthropogenic and Natural Origin Organohalogen Compounds in Three Porpoise Species.
    Ochiai M, Nomiyama K, Isobe T, Yamada T.K, Tajima Y, Matsuishi T, Amano M, Tanabe S Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 73 1709 -1712 2011年 [査読有り]
     
    Proceedings
  • 野見山桂, 江口哲史, 水川葉月, 磯部友彦, 山田格, 田辺信介 日本水環境学会シンポジウム講演集 13th 240 2010年09月
  • 野見山桂, 落合真理, 江口哲史, 水川葉月, 村田仁子, 山田格, 高橋真, 田辺信介 環境化学討論会講演要旨集 19th 248 -249 2010年06月
  • 江口哲史, 野見山桂, SUBRAMANIAN Annamalai, BULBULE Keshav A, PARTHASARATY Peethmbaram, 高橋真, 田辺信介 環境化学討論会講演要旨集 19th 242 -243 2010年06月
  • 落合真理, 野見山桂, 水川葉月, 江口哲史, 高橋真, 松石隆, 山田格, 田辺信介 環境化学討論会講演要旨集 19th 244 -245 2010年06月
  • Accumulation features of hydroxylated polyblominated diphenyl ethers (OH-PBDEs) in the blood of toothed and baleen whales stranded at Japanese coastal waters.
    Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 72 864 -867 2010年 [査読有り]
  • Accumulation features of anthorogenic and naturally produced organohalogen compounds in human serum from Indian E-waste recycling workers and residents near coastal areas
    Organohalogen Compounds 72 868 -871 2010年
  • Accumulation features of PCBs and hydroxylated metabolites in the blood of terrestrial mammals
    Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 72 944 -947 2010年 [査読有り]
  • Accumulation features of anthorogenic and naturally produced organohalogen compounds in human serum from Indian E-waste recycling workers and residents near coastal areas
    Organohalogen Compounds 72 868 -871 2010年
  • Polychlorinated biphenyls (PCBs) and hydroxylated PCBs (OH-PCBs) in three porpoise species: Accumulation features and metabolic capacity
    Organohalogen Compounds 72 1027 -1030 2010年
  • Accumulation Features of Hydroxylated-PCBs (OH-PCBs) in the Blood of Pigs Collected from a Dumping Site for Municipal Wastes in India
    Mizukawa H, Nomiyama K, Kunisue T, Watanabe M.X, Subramanian A, Takahashi S, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 4 175 -181 2010年 [査読有り]
     
    Proceedings
  • Organohalogen and Metabolite Contaminants in Human Serum Samples from Indian E-Waste Recycling Workers
    Eguchi A, Nomiyama K, Subramanian A, Parthasarathy P, Bulbule K.A, Takahashi S, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 4 167 -174 2010年 [査読有り]
     
    Proceedings
  • Polychlorinated Biphenyls and Hydroxylated Polychlorinated Biphenyls in the Blood of Toothed and Baleen Whales Stranded along Japanese Coastal Waters.
    Nomiyama, K, Murata, S, Kunisue, T, Yamada, T.K, Mizukawa, H, Takahashi, S, Tanabe, S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 4 67 -74 2010年 [査読有り]
     
    Proceedings
  • Comprehensive study on hydroxylatd polychlorinated biphenyls in the blood of cetaceans, including toothed whales and baleen whales stranded at the Japanese coast.
    Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 71 53 -58 2009年 [査読有り]
  • Accumulation features of hydroxylated-PCBs (OH-PCBs) in the blood of pigs collected from dumping site for municipal wastes in India
    Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 71 1860 -1864 2009年 [査読有り]
  • Hysroxylated polychlorinated biphenyls in the blood of Baikal seals (Pusa Sibirica)
    Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 70 1475 -1478 2008年 [査読有り]
  • Hydroxylated polychlorinated biphenyls in the blood of cetaceans stranded along the Japanes coast.
    Murata, S, Nomiyama, K, Kunisue, T, Takahashi, S, Yamada, T.K, Tanabe, S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 2 55 -66 2008年 [査読有り]
     
    Proceedings
  • Residue levels, of OH-PCBs and PCBs in the blood of Baikal seals (Pusa Sibirica)
    Imaeda, D, Nomiyama, K, Kunisue, T, Iwata, H, Tsydenova, O, Takahashi, S, Amano, M, Petrov, E.A, Batoev, V.B, Tanabe, S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 2 111 -117 2008年 [査読有り]
     
    Proceedings
  • Production Mechanism of hydroxylated polychlorinated biphenyls (OH-PCBs) by oxidative degradation of PCBs using titanium dioxide in water, and endocrine disrupting effects of their OH-PCBs
    Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 69 2968 -2971 2007年 [査読有り]
  • Kei Nomiyama, Teiji Tanizaki, Koji Arizono, Ryota Shinohara CHEMOSPHERE 66 (6) 1138 -1145 2007年01月 
    The oxidative photodegradation behaviors of selected three coplanar polychlorinated biphenyls (PCBs), (CB77, C1381, and C13169) using titanium dioxide (TiO2) in water were investigated. The main purposes were to clarify the structural relation between the original PCBs and the intermediates derived by TiO2 Oxidation and to evaluate the estrogenic and thyroid hormonal activity in the treated three coplanar PCBs during the oxidative reactions. Approximately 90% of the three coplanar PCBs decomposed within 180 min. Intermediates from the decomposition of the three coplanar PCBs, such as some hydroxylated-PCBs (OH-PCBs), carboxylic intermediates, phenolic intermediates, and other intermediates produced by the cleavage of a benzene ring were identified and quantified. In the degradation pathways, the produced amounts of OHPCBs increased within 60 min of irradiation time. The estrogenic activity and thyroid hormonal activity of the intermediates from the three coplanar PCBs in water at 0, 60, 120, 180 and 240 min of irradiation time were assessed by using a yeast two-hybrid assay system for human estrogen receptor alpha (hER alpha) and human thyroid hormone receptor alpha (hTR alpha). The maximal estrogenic activities were induced by the solutions of decomposed PCBs with irradiation time at 60-120 min similar and slightly lower than those after the irradiation time. We found that the solutions occuring during the irradiation times of 60-120 min contained several 4-OH-PCBs substituted with OH and Cl at para- and para'-positions having estrogenic activity. The thyroid hormonal activity was not detected in the decomposed three coplanar PCBs solutions. (c) 2006 Elsevier Ltd. All rights reserved.
  • K Nomiyama, T Tanizaki, H Ishibashi, K Arizono, R Shinohara ENVIRONMENTAL SCIENCE & TECHNOLOGY 39 (22) 8762 -8769 2005年11月 
    The oxidative photodegradation behaviors of selected polychlorinated biphenyls (PCBs) [2,3,3',4'-tetraCB (BZ number: CB56), 2,3',4',5-tetra (CB70), and 3,4,4',5-tetraCB (CB81: coplanar PCB)] using titanium dioxide (TiO2) in water were investigated. The main purposes were to clarify the structural relation between the original PCBs and the intermediates derived by TiO2 oxidation and to evaluate the estrogenic activity in the treated PCBs during the oxidative reactions. Approximately 90% of the three tetraCBs decomposed within 120 min. Intermediates by decomposition of three tetraCBs, such as some OH-tetraCBs and OH-triCBs, carboxylic intermediates, phenolic intermediates, and other intermediates produced by the cleavage of a benzene ring were identified and quantified. In the degradation pathways, the produced amounts of OH-tetraCB and OH-triCB increased within 60 min of irradiation time. Estrogenic activities of the intermediates from the three tetraCBs in water were assessed by using a yeast two-hybrid assay system for human estrogen receptor alpha (hER alpha). The maximal estrogenic activities were induced by the solutions of decomposed CB81 with irradiation time at 60 min. We found that the solutions at an irradiation time of 60-120 min contained several 4-OH-tetraCBs and 4-OH-triCBs substituted with OH and Cl at para- and para'-positions. It is presumed that the chemical structures of the 4-OH-PCBs are similar to that of 17 beta-estradiol (beta-E2); these intermediates present strong estrogenic activities. Moreover, we learned that there is a high possibility of conversion from some low toxic PCBs congeners to strong estrogenic OH-PCBs.
  • 森大樹, 松岡宗和, 野見山桂, 高尾雄二, 高良真也, 篠原亮太, 武政剛弘, 有薗幸司 日本内分泌かく乱化学物質学会研究発表会要旨集 8th 167 -167 2005年09月
  • 川角祐介, 石橋弘志, 野見山桂, 森大樹, 寺田正光, 高尾雄二, 篠原亮太, 古賀実, 有薗幸司 日本内分泌かく乱化学物質学会研究発表会要旨集 7th 211 2004年12月

受賞

  • 2018年04月 愛媛大学理学部 ベストレクチャー賞
     
    受賞者: 野見山 桂
  • 2018年04月 愛媛県、三浦工業 14回三浦環境賞奨励賞
     
    受賞者: 野見山 桂
  • 2016年04月 Sony World Photography Awards Photographer of the Year
     
    受賞者: 野見山 桂
  • 2016年 International Photography Awards 1st Place, Nature Underwater category
     
    受賞者: 野見山 桂
  • 2009年 日本環境毒性学会・バイオアッセイ研究会・奨励賞
     JPN
  • 2007年 日本水環境学会・博士研究奨励賞(オルガノ賞)
     JPN

共同研究・競争的資金等の研究課題

委員歴

  • 2018年04月 - 現在   日本内分泌撹乱化学物質学会   評議員
  • 2018年 - 現在   Dioxin2019   実行委員
  • 2017年 - 現在   日本環境化学会   国際企画部会 副幹事
  • 2017年 - 現在   日本環境化学会   POPs部会 副幹事
  • 2016年04月 - 現在   文部科学省共同利用・共同研究拠点形成「化学汚染・沿岸環境研究拠点(LaMer)   事業推進担当者
  • 2016年04月 - 現在   日本環境化学会   評議員
  • 2014年 - 現在   日本環境化学会   学会実行委員
  • 2016年04月 - 2019年05月   水環境学会   中国・四国支部会幹事長
  • 2016年05月 - 2017年05月   PRIMO19   学会実行委員
  • 2014年 - 2014年   ICAEC2014   実行委員
  • 2008年04月 - 2012年03月   文部科学省グローバルCOEプログラム「化学物質の環境科学教育研究拠点」   事業推進担当者
  • 2012年 - 2012年   SETAC Asia/Pacific 2012   実行委員

担当経験のある科目

  • 化学英語II愛媛大学理学部
  • 有害物質動態論愛媛大学大学院理工学研究科
  • 化学実験II & III愛媛大学理学部
  • 化学実験I愛媛大学理学部
  • 新入生セミナーB愛媛大学理学部
  • 基礎化学実験愛媛大学理学部
  • 生物環境保全学演習Ⅰ& II愛媛大学農学部
  • 生物環境保全学実験Ⅰ& II愛媛大学農学部
  • 生物環境保全学研究愛媛大学農学部

社会貢献活動

  • 地球規模で考える環境汚染 ~ 環境汚染が私達の暮らしを壊す。 環境について考えてみませんか ~
    期間 : 2018年09月
    役割 : 講師
    主催者・発行元 : COOP松山
  • 化学物質による野生生物の汚染と 環境ホルモン問題について
    期間 : 2018年05月
    役割 : 講師
    主催者・発行元 : 愛媛大学、三重中学
  • 自然写真を通して環境問題を考える
    期間 : 2018年03月
    役割 : 講師
    主催者・発行元 : 愛媛大学後援会
  • 出張講義
    期間 : 2017年11月29日 - 2017年11月29日
    役割 : 講師
    主催者・発行元 : 聖カタリナ大学
  • Chemical Hazard Symposium
    期間 : 2017年08月02日
    役割 : 出演
  • 期間 : 2017年06月30日 - 2017年07月03日
    役割 : 編集
    主催者・発行元 : 愛媛大学
  • 出張講義
    期間 : 2016年11月 - 2016年11月
    役割 : 講師
    主催者・発行元 : 聖カタリナ大学
  • 化学物質による鯨類への脅威
    期間 : 2016年01月26日
    役割 : 講師
    主催者・発行元 : 熊本大学、熊本県立大学ダイビング部
    イベント・番組・新聞雑誌名 : 「球美とクジラ」 講演会
  • 出張講義
    期間 : 2015年11月 - 2015年11月
    役割 : 講師
    主催者・発行元 : 聖カタリナ大学
  • 化学物質によるペットの汚染問題
    期間 : 2014年12月
    役割 : 講師
    主催者・発行元 : 聖カタリナ大学
  • 化学物質による野生生物汚染と環境ホルモン問題について
    期間 : 2014年05月
    役割 : 講師
    主催者・発行元 : 愛媛大学、三重中学
  • 四国自然史科学研究センター設立10周年記念イベント シンポジウム「四国の自然は、いま 2012」
    期間 : 2012年12月16日
    役割 : 出演
    主催者・発行元 : 四国自然史科学研究センター

メディア報道

愛媛大学教員活動実績

教育活動(B)

担当授業科目(B01)

  • 2019, 前期, 学部, 卒業研究Ⅰ
  • 2019, 前期, 修士, 分子科学高等実習Ⅱ
  • 2019, 前期, 修士, 化学ゼミナールⅢ


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