研究者データベース

島﨑 洋次シマザキ ヨウジ

所属部署名大学院理工学研究科 環境機能科学専攻
職名准教授
Last Updated :2019/10/19

研究者基本情報

基本情報

氏名

  • 氏名

    島﨑 洋次
  • 氏名(カナ)

    シマザキ ヨウジ

基本情報

所属

所属・職名

  • 部署

    大学院理工学研究科 環境機能科学専攻
  • 職名

    准教授

学歴等

学位

  • 博士(理学)

その他基本情報

所属学協会

  • クロマトグラフィー科学会
  • 日本動物学会
  • 日本比較生理生化学会
  • 日本分析化学会
  • 日本電気泳動学会
  • 日本化学会

委員歴

  • 2016年04月 - 現在, 日本電気泳動学会, 理事
  • 2014年04月 - 現在, 日本電気泳動学会, 評議員
  • 2019年03月 - 2020年02月, 日本化学会中国四国支部, 化学教育協議会委員
  • 2019年01月 - 2019年10月, 日本化学会, 第9回CSJフェスタ実行委員
  • 2018年04月 - 2020年02月, 日本化学会中国四国支部, 支部幹事
  • 2004年 - 2004年, 日本分析化学会, 中国四国支部 庶務幹事, 日本分析化学会
  • 2011年 - 現在, 日本分析化学会, 中国四国支部幹事, 日本分析化学会

経歴

  • 2007年04月 - 現在, - 愛媛大学大学院 理工学研究科(准教授)
  • 1996年01月 - 2007年, 愛媛大学理学部(助手)
  • 1994年04月 - 1995年, 三菱化学生命科学研究所(特別研究員)

研究活動情報

研究分野等

研究分野

  • 複合化学, 分析化学
  • 生物科学, 機能生物化学

研究キーワード

  • タンパク質
  • 酵素
  • 活性
  • 構造
  • 電気泳動法
  • 質量分析
  • プロテオミックス

著書・発表論文等

論文

  • Analysis of the degradation of amyloid beta fibrils after separation via the combination of non-denaturing agarose electrophoresis and Congo red dye staining, Kawano Risa, Nakanishi Ayaka, Zako Tamotsu, SHIMAZAKI Youji, Separation Science Plus, 2019年09月, [査読有り]
  • Removal of copper in microdroplets by ovomucoid hydrolysates bound to reverse-phase chromatography media within pipette tips, Shimazaki Youji, Inoue Suzuka, Applied Biochemistry and Biotechnology, 2019年09月, [査読有り], 10.1007/s12010-019-03050-w
  • Microscale isolation of native forms of lysozyme from chicken egg white by gel isoelectric focusing, Youji Shimazaki , Yoshiko Ochi, Kennosuke Fujimura, Electrophoresis, 2018年04月, [査読有り], 10.1002/elps.201700445
  • Antibacterial activity of lysozyme-binding proteins from chicken egg white, SHIMAZAKI Youji, Ayumi Takahashi, Journal of Microbiological Methods, 2018年11月, [査読有り]
  • Enzyme inhibitory activity of ovomucoid extracted using a carboxypeptidase Y-immobilized membrane, Shimazaki Youji, Kosuke Tanaka, Keisuke Sakata, CHROMATOGRAPHY, 2018年02月, [査読有り], 10.15583/jpchrom.2017.020
  • Hydrolytic activity of esterase-antibody complexes retained within gel capsules after complex isolation, Shimazaki Youji, Miyatsuka Rino, Applied Biochemistry and Biotechnology, 2017年07月, [査読有り], 10.1007/s12010-016-2393-0
  • Retaining activity of enzymes after capture and extraction within a single-drop of biological fluid using immunoaffinity membranes, Shimazaki Youji, Sato Yuki, Journal of Chromatography B, 2016年05月, [査読有り], 10.1016/j.jchromb.2015.12.054.
  • Inhibition of amyloid beta protein fibrillation via carboxypeptidase Y after protein trapping using imunoaffinity membranes, Saito Masaki, Zako Tamotsu, Takahashi Ryouji and Shimazaki Youji, Chemistry Letters, 2016年, [査読有り], 10.1246/cl.160613
  • Combined method of immunoaffinity membrane within tubes and MALDI-TOF MS for capturing and analyzing amyloid beta, Shimazaki Youji, Takatsu Yoko, Applied Biochemistry and Biotechnology, 2015年09月, [査読有り], 10.1007/s12010-015-1837-2.
  • Micro-scale and analysis of intact carboxylesterase after trapping on an immunoaffinity membrane surface, Kimura Ayaka, SHIMAZAKI Youji, Applied Biochemistry and Biotechnology, 2014年, [査読有り], 10.1007/s12010-014-0807-4.
  • A microfluidic device containing membrane-immobilized antibodies for successively capturing cytosolic enzymes, SHIMAZAKI Youji, Hashimoto Ai, Talanta, 2014年, [査読有り], 10.1016/j.talanta.2014.03.001.
  • Isolation and analysis of amyloid-beta 1-42 monomer and oligomer in liquid droplets using an immunoaffinity membrane, SHIMAZAKI Youji, Hirose Yu, Journal of Chromatography B, 2014年, [査読有り], 10.1016/j.jchromb.2014.09.021.
  • Analysis of enzyme activity regulation by non-denaturing electrophoresis and application of this regulation for enzyme reactor production, SHIMAZAKI Youji, Miki Shizuka, Journal of Enzyme inhibition and Medical Chemistry, 2013年, [査読有り], 10.3109/14756366.2012.693918.
  • Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane, SHIMAZAKI Youji, Nishimura Yuri, Saito Masaki, Journal of Phamaceutical and Biomedical Analysis, 2013年, [査読有り], 10.1016/j.jpba.2013.05.031.
  • Analysis of trypsin inhibition activity in human plasma protein after separation by non-denaturing electrophoresis, SHIMAZAKI Youji, Michhiro Madoka, Clinca Chimica Acta, 2013年, [査読有り], 10.1016/j.cca.2013.07.004.
  • Analysis of activity of esterase captured onto an immunoaffinity membrane, SHIMAZAKI Youji, Sakikawa Takahiro, Kimura Ayaka, Clinica Chimica Acta, 2012年, [査読有り], 10.1016/j.cca.2011.10.015.
  • Successive analysis of antigen trapping and enzymatic digestion on membrane-immobilized avidin, SHIMAZAKI Youji, Kohno Yoshinori, Analytical Biochemistry, 2012年, [査読有り], 10.1016/j.ab.2011.12.023.
  • Epitope analysis using membrane-immobilized avidin and Protein A, SHIMAZAKI Youji, Kohno Yoshinori, Fukui Izumi, Koyashiki Toshiharu, Protein Expression and Purification, 2012年, [査読有り], 10.1016/j.pep.2012.03.017.
  • Enzymolysis of high density lipoprotein with a combination of membrane-immobilized esterase and trypsin, Sakikawa Takahiro, SHIMAZAKI Youji, Journal of Phamacutical and Biomedical Analysis, 2012年, [査読有り], 10.1016/j.jpba.2012.07.032.
  • Direct trapping and analysis of hemoglobin in flowing fluid using membrane-immobilized anti-haptoglobin antibody, Shimazaki Youji, Kimura Ayaka, Journal of Pharmaceutical and Biomedical Analysis, 2011年, 10.1016/j.jpba.2011.07.027.
  • Reversible inhibition of esterase activity after separation and immobilization., Sakikawa Takahiro, Shimazaki Youji, Applied Biochemistry and Biotechnology, 2011年, [査読有り], 10.1007/s12010-011-9233-z.
  • Simultaneous production of immunoaffinity membranes, SHIMAZAKI Youji, MIYAMOTO Masayuki, Journal of Chromatography B, 2010年, [査読有り], 1570-0232, 10.1016/j.jchromb.2010.08.042
  • Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis., SHIMAZAKI Youji, SHIMAZAKI Youji, SHIMIZU Kenta, MASAOKA Suzuna, Elsevier ScienceTalanta, 2010年, [査読有り], 0039-9140, 10.1016/j.talanta.2010.06.028
  • Determination of malic acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with Ponceau S, Shimazaki Youji, Sakikawa Takahiro, Bentham ScienceProtein and Peptide Letters, 2010年, [査読有り], 10.2174/092986610791498867
  • Maintenance of esterase activity and immunoreactivity after colloidal silver staining of non-denaturing proteins, Shimazaki Youji, Horikawa Seigo, Elsevier ScienceClinica Chimica Acta, 2010年, [査読有り], 0009-8981, 10.1016/j.cca.2010.03.017
  • Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane, SHIMAZAKI Youji, KURODA Takahiro, Biotechnology Letters, 2009年, [査読有り], 0141-5492, 10.1007/s10529-009-0041-2
  • Production of immunoaffinity membranes for direct analysis of antigen after antibody separation and blotting under non-denaturing conditions., SHIMAZAKI Youji, KODAMA Azusa, Analytica Chimica Acta, 2009年, [査読有り], 0003-2670, 10.1016/j.aca.2009.04.007
  • Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis, SHIMAZAKI Youji, Watanabe Sono, Clinica Chimica Acta, 2008年, [査読有り], 10.1016/j.cca.2007.12.017
  • Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis, SHIMAZAKI Youji, KADOTA Mariko, Analytica Chimica Acta, 2008年, [査読有り], 0003-2670, 10.1016/j.aca.2008.04.046
  • Direct analysis of retinal dehydrogenase activity on an electroblotting membrane following separation by non-denaturing two-dimensional electrophoresis, SHIMAZAKI Youji, KURODA Takahiro, Elsevier ScienceJournal of Chromatography B, 2007年, [査読有り], 1570-0232, 10.1016/j.jchromb.2007.10.028
  • Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis., SHIMAZAKI Youji, MIYAMOTO Masayuki, Biotechnology and Bioengineering, 2007年, [査読有り], 0006-3592, 10.1002/bit.21437
  • Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis., Shimazaki Youji, Journal of Biochemical and Biophysical Methods, 2007年, [査読有り], 10.1016/j.jbbm.2006.11.010
  • Analysis of hydrolytic activity of phospholipase Cα from porcine retina on retinyl ester and phosphatidylcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry., SHIMAZAKI Youji, KISHI Haruka, MORI Masami, YASUDA Chiyo, MANABE Takashi, Journal of Chromatography B, 2006年, [査読有り], 1570-0232, 10.1016/j.jchromb.2006.05.038
  • Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents., Shimazaki Youji, Manabe Takashi, Biochimica et Biophysica Acta, 2005年, [査読有り], 10.1016/j.bbapap.2005.02.011
  • Activity and sequence structure analysis of cytosolic dehydrogenase by mass spectrometry after separation by nondenaturing two-dimensional electrophoresis., SHIMAZAKI Y, SUGAWARA Y, Analytical Biochemistry, 2004年, [査読有り], 0003-2697, 10.1016/j.ab.2004.01.028
  • Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver, Shimazaki Youji, Sugawara Yuki, Manabe Takashi, Proteomics, 2004年, [査読有り], 10.1002/pmic.200300702
  • Simultaneous analysis of esterase and transferase activities in cytosol proteins from the bovine retina by using microscale non-denaturing two-dimensional electrophoresis., Shimazaki Youij, Hiraka Yoko, Uesugi Minatsu, Biochimica et Biophysica Acta, 2004年, [査読有り], 10.1016/j.bbapap.2003.09.007
  • Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis., Proteomics, 2003年, [査読有り], 10.1002/pmic.200300467
  • 未変性条件の二次元電気泳動法による水溶性タンパク質の酵素活性解析, 島崎洋次、平加容子、大西弘美、真鍋敬, 分析化学, 2002年, [査読有り], 10.2116/bunsekikagaku.51.367
  • Simple quantification of Cu, Zn-super oxide dismutase activity after separation by non-denaturing isoelectric focusing, Biochimica et Biophysica Acta, 2002年, [査読有り], 10.1016/S0304-4165(02)00259-3
  • Determination of the enzyme for the fragmentation of the third component of complement using two-dimensional electrophoresis., Shimazaki Youji, Muro Masayoshi, Manabe Takashi, Jpn J Electroph., 2001年, [査読有り], 0031-9082|1349-9785, 10.2198/sbk.45.89
  • Isolation of the third component of complement and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster., Shimazaki Youji , Maeyama Kazutaka, Fujii Tamotsu, Developmental Comparative Immunology., 2001年, [査読有り], 10.1016/S0145-305X(01)00007-6
  • Two distinct mechanisms underlie the stimulation of neurotransmitter release by phorbol esters in clonal rat pheochromocytoma PC12 Cells., Iwasaki Satoshi, Kataoka Masakazu, SEkiguchi Mariko, Shimazaki Yoji, Sato Kazuki, Takahashi Masami, Journal Biochemistry, 2000年, [査読有り]
  • Detection and identification of constitutive polypeptides of the third component of complement using microscale two-dimensional electrophoresis, Shimazaki Youji, Fujiwara Mai, Manabe Takashi, Jpn J Electroph., 2000年, [査読有り], 0031-9082|1349-9785, 10.2198/sbk.44.21
  • Selection of an effective enzyme for digestion of non-denaturing proteins using microscale two-dimensional electrophoresis., Shimazaki Youji, Muro Masayuki, Manabe Takashi, Clinica Chimica Acta, 2000年, [査読有り], 10.1016/S0009-8981(00)00329-6
  • Protein spot recognition on the non-denaturing and denaturing two-dimensional electrophoresis patterns using in situ immunosubtraction via Protein A agarose and antibodies ., SHIMAZAKI Y, OHARA S, MANABE T, Journal of biochemical and biophysical methods, 1999年, [査読有り], 0165-022X, 10.1016/S0165-022X(99)00014-7
  • Localization of a visual Gg protein in the photoreceptors of a polychaete, Perinereis brevicirris (Annelida)., Miyako-Shimazaki Yumiko, Shimazaki Youji, Ohtsu Kohzoh, Yamamoto Masamichi, Cell Tissue Research, 1999年, [査読有り], 10.1007/s004410051302
  • Application of two-dimensional electrophoresis without denaturing agents to comparative study of vertebrate plasma proteins., Shimazaki Youji, Manabe Takashi, Japanese Journal of electrophoresis, 1998年, 0031-9082|1349-9785, 10.2198/sbk.42.13
  • Separation of chicken egg proteins using two-dimensional electrophoresis in the absence of denaturing agents., Shimazaki Youi, Manabe Takashi, Japanese Journal of electrophoresis, 1998年, 0031-9082|1349-9785, 10.2198/sbk.42.155
  • Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies., SHIMAZAKI Y, OHARA S, MANABE T, Journal of biochemical and biophysical methods., 1998年, [査読有り], 0165-022X, 10.1016/S0165-022X(98)00004-9
  • Effect of formoguanamine on structure and function of the compound eye of the butterfly, Papilio xuthus., Miyako-Shimazaki Yumiko, Shimazaki Youji, Eguchi Eisuke, Zoological Science, 1997年, [査読有り], 0289-0003, 10.2108/zsj.14.29
  • Phosphorylation of 25-KDa Synaptosome-associated protein., Shimazaki Youji, Nishiki Tei-ichi, Omori Akira, Sekiguchi Mariko, Kamata Yoichi, Kozaki Shunji, Takahashi Masami, The Journal of Biological Chemistry, 1996年, [査読有り], 10.1074/jbc.271.24.14548
  • Light-dependent metabolic pathway of 3-hydroxy retinoids in the eye of a butterfly, Papilio xuthus., Shimazaki Youji, Eguchi Eisuke, Journal of Comparative Physiology A, 1995年, [査読有り]
  • Synthesis of 3-hydroxyretinal in the Cytosol of the butterfly compound eye., Shimazaki Youji, Eguchi Eisuke, Vision Research, 1993年, [査読有り]
  • Changes in rhabdom volumes of isolated compouned eyes of the crab, Hemigrapsus sanguineus-A study in correlation with a circadian rhythm., Eguchi Eisuke, Shimazaki Youji, Suzuki Tatsuo, Zoological Science, 1991年, [査読有り]

講演・口頭発表等

  • 種々色素とネイティブ電気泳動法の組み合わせによるタンパク質及びタンパク質凝集体のネイティブ分離分析法の検討, O島崎洋次、井上蒼士、足利諒、座古保, 日本分析化学会第68年会, 2019年09月
  • 非変性条件アガロースゲル電気泳動法とコンゴーレッドによるアミロイドβ凝集体の分離分析法の構築, 島崎洋次、川野莉沙、足利諒、座古保, 第70回日本電気泳動学会総会, 2019年07月
  • 生体酵素によるアミロイドβ凝集体の脱凝集過程分析法の構築, Ο川野莉沙、中西文香、福永隼大、座古保、島﨑 洋次, 2018年日本化学会中国四国支部大会(愛媛大会), 2018年11月
  • ウズラ卵白タンパク質の大腸菌に対する抗菌活性分析法の開発, Ο原田靖大、井上涼香、島崎洋次, 2018年日本化学会中国四国支部大会(愛媛大会), 2018年11月
  • ニワトリとウズラ卵白リゾチーム存在状態の解析, 原田靖大、井上涼香、O島﨑 洋次, 第69回日本電気泳動学会総会, 2018年08月
  • 卵白中のリゾチーム複合体の電気泳動分離とその活性分析法の構築, 高橋歩実, O島﨑 洋次, 第78回分析化学討論会, 2018年05月
  • ミクロ非変性等電点電気泳動法と培養技術を組み合わせたリゾチーム複合体の活性解析, 高橋歩実,中野令菜, 李海月,o島崎洋次, 第68回日本電気泳動学会総会, 2017年11月
  • 非変性条件の等電点電気泳動法による卵白リゾチームのインタクト分離溶出法の検討, o島崎洋次、藤村建之介, 第77回分析化学討論会, 2017年05月

MISC

  • 分離. 電気泳動, 島崎洋次、真鍋敬, ぶんせき(日本分析化学会), 6, 301, 302, 2001年

その他研究情報

競争的資金



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