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島﨑 洋次シマザキ ヨウジ

所属部署
大学院理工学研究科 環境機能科学専攻
職名准教授
メールアドレスshimazaki.yoji.my[at]ehime-u.ac.jp ※[at]を@に書き換えて送信して下さい
ホームページURLhttp://chem.sci.ehime-u.ac.jp/~anachem1/
生年月日
Last Updated :2017/08/18

研究者基本情報

経歴

  •   2007年04月,  - 現在, - 愛媛大学大学院 理工学研究科, 准教授
  •   1997年01月,  - 2007年03月, 愛媛大学理学部, 助手
  •   1995年,  - 1996年, 三菱化学生命科学研究所, 特別研究員

所属学協会

  • 日本動物学会
  • 日本比較生理生化学学会
  • 日本分析化学会
  • 日本電気泳動学会
  • 日本化学会

委員歴

  •   2016年04月 - 現在, 日本電気泳動学会, 理事
  •   2014年04月 - 現在, 日本電気泳動学会, 評議員
  •   2004年 - 2004年, 日本分析化学会, 中国四国支部 庶務幹事
  •   2011年 - 現在, 日本分析化学会, 中国四国支部幹事

研究活動情報

研究分野

  • 分析化学
  • 機能生物化学

研究キーワード

    タンパク質, 酵素, 活性, 構造, 電気泳動法, 質量分析, プロテオミックス

論文

  • Hydrolytic activity of esterase-antibody complexes retained within gel capsules after complex isolation
    Shimazaki Youji, Miyatsuka Rino, Applied Biochemistry and Biotechnology, 182, (in press) 1208 - 1217,   2017年
  • Retaining activity of enzymes after capture and extraction within a single-drop of biological fluid using immunoaffinity membranes
    Shimazaki Youji, Sato Yuki, Journal of Chromatography B, 1021, 108 - 113,   2016年05月
  • Inhibition of amyloid beta protein fibrillation via carboxypeptidase Y after protein trapping using imunoaffinity membranes
    Saito Masaki, Zako Tamotsu, Takahashi Ryouji and Shimazaki Youji, Chemistry Letters, 45, 1241 - 1243,   2016年
  • Combined method of immunoaffinity membrane within tubes and MALDI-TOF MS for capturing and analyzing amyloid beta
    Shimazaki Youji, Takatsu Yoko, Applied Biochemistry and Biotechnology, 177, 1565 - 1571,   2015年09月
  • Micro-scale and analysis of intact carboxylesterase after trapping on an immunoaffinity membrane surface
    Kimura Ayaka, SHIMAZAKI Youji, Applied Biochemistry and Biotechnology, 172, 4053 - 4061,   2014年
  • A microfluidic device containing membrane-immobilized antibodies for successively capturing cytosolic enzymes
    SHIMAZAKI Youji, Hashimoto Ai, Talanta, 125, 400 - 404,   2014年
  • Isolation and analysis of amyloid-beta 1-42 monomer and oligomer in liquid droplets using an immunoaffinity membrane
    SHIMAZAKI Youji, Hirose Yu, Journal of Chromatography B, 972, 53 - 57,   2014年
  • Analysis of enzyme activity regulation by non-denaturing electrophoresis and application of this regulation for enzyme reactor production
    SHIMAZAKI Youji, Miki Shizuka, Journal of Enzyme inhibition and Medical Chemistry, 28, (5) 894 - 899,   2013年
  • Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane
    SHIMAZAKI Youji, Nishimura Yuri, Saito Masaki, Journal of Phamaceutical and Biomedical Analysis, 83, 293 - 298,   2013年
  • Analysis of trypsin inhibition activity in human plasma protein after separation by non-denaturing electrophoresis
    SHIMAZAKI Youji, Michhiro Madoka, Clinca Chimica Acta, 425, 48 - 53,   2013年
  • Analysis of activity of esterase captured onto an immunoaffinity membrane
    SHIMAZAKI Youji, Sakikawa Takahiro, Kimura Ayaka, Clinica Chimica Acta, 413, 269 - 272,   2012年
  • Successive analysis of antigen trapping and enzymatic digestion on membrane-immobilized avidin
    SHIMAZAKI Youji, Kohno Yoshinori, Analytical Biochemistry, 422, 55 - 57,   2012年
  • Epitope analysis using membrane-immobilized avidin and Protein A
    SHIMAZAKI Youji, Kohno Yoshinori, Fukui Izumi, Koyashiki Toshiharu, Protein Expression and Purification, 83, 177 - 181,   2012年
  • Enzymolysis of high density lipoprotein with a combination of membrane-immobilized esterase and trypsin
    Sakikawa Takahiro, SHIMAZAKI Youji, Journal of Phamacutical and Biomedical Analysis, 71, 179 - 182,   2012年
  • Direct trapping and analysis of hemoglobin in flowing fluid using membrane-immobilized anti-haptoglobin antibody
    Shimazaki Youji, Kimura Ayaka, Journal of Pharmaceutical and Biomedical Analysis, 56, 1085 - 1088,   2011年
  • Reversible inhibition of esterase activity after separation and immobilization.
    Sakikawa Takahiro, Shimazaki Youji, Applied Biochemistry and Biotechnology, 165, 69 - 74,   2011年
  • Simultaneous production of immunoaffinity membranes
    SHIMAZAKI Youji, MIYAMOTO Masayuki, Journal of Chromatography B, 878, (28) 2852 - 2856,   2010年
  • Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis.
    SHIMAZAKI Youji, SHIMAZAKI Youji, SHIMIZU Kenta, MASAOKA Suzuna, Elsevier ScienceTalanta, 82, (3) 1063 - 1067,   2010年
  • Determination of malic acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with Ponceau S
    Shimazaki Youji, Sakikawa Takahiro, Bentham ScienceProtein and Peptide Letters, 17, 1048 - 1052,   2010年
  • Maintenance of esterase activity and immunoreactivity after colloidal silver staining of non-denaturing proteins
    Shimazaki Youji, Horikawa Seigo, Elsevier ScienceClinica Chimica Acta, 411, (13-14) 992 - 993,   2010年
  • Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane
    SHIMAZAKI Youji, KURODA Takahiro, Biotechnology Letters, 31, (10) 1545 - 1549,   2009年
  • Production of immunoaffinity membranes for direct analysis of antigen after antibody separation and blotting under non-denaturing conditions.
    SHIMAZAKI Youji, KODAMA Azusa, Analytica Chimica Acta, 643, (1-2) 61 - 66,   2009年
  • Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis
    SHIMAZAKI Youji, Watanabe Sono, Clinica Chimica Acta, 390, 145 - 147,   2008年
  • Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis
    SHIMAZAKI Youji, KADOTA Mariko, Analytica Chimica Acta, 618, (1) 116 - 119,   2008年
  • Direct analysis of retinal dehydrogenase activity on an electroblotting membrane following separation by non-denaturing two-dimensional electrophoresis
    SHIMAZAKI Youji, KURODA Takahiro, Elsevier ScienceJournal of Chromatography B, 860, (2) 180 - 184,   2007年
  • Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis.
    SHIMAZAKI Youji, MIYAMOTO Masayuki, Biotechnology and Bioengineering, 98, (4) 732 - 736,   2007年
  • Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis.
    Shimazaki Youji, Journal of Biochemical and Biophysical Methods, 70, 487 - 491,   2007年
  • Analysis of hydrolytic activity of phospholipase Cα from porcine retina on retinyl ester and phosphatidylcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry.
    SHIMAZAKI Youji, KISHI Haruka, MORI Masami, YASUDA Chiyo, MANABE Takashi, Journal of Chromatography B, 843, (1) 42 - 46,   2006年
  • Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents.
    Shimazaki Youji, Manabe Takashi, Biochimica et Biophysica Acta, 1749, 95 - 101,   2005年
  • Activity and sequence structure analysis of cytosolic dehydrogenase by mass spectrometry after separation by nondenaturing two-dimensional electrophoresis.
    SHIMAZAKI Y, SUGAWARA Y, Analytical Biochemistry, 328, (1) 87 - 89,   2004年
  • Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver
    Shimazaki Youji, Sugawara Yuki, Manabe Takashi, Proteomics, 4, 1406 - 1411,   2004年
  • Simultaneous analysis of esterase and transferase activities in cytosol proteins from the bovine retina by using microscale non-denaturing two-dimensional electrophoresis.
    Shimazaki Youij, Hiraka Yoko, Uesugi Minatsu, Biochimica et Biophysica Acta, 1696, 51 - 57,   2004年
  • Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis.
    Proteomics, 3, 2002 - 2007,   2003年
  • 未変性条件の二次元電気泳動法による水溶性タンパク質の酵素活性解析
    島崎洋次、平加容子、大西弘美、真鍋敬, 分析化学, 51, (6) 367 - 371,   2002年
  • Simple quantification of Cu, Zn-super oxide dismutase activity after separation by non-denaturing isoelectric focusing
    Biochimica et Biophysica Acta, 1571, 245 - 248,   2002年
  • Determination of the enzyme for the fragmentation of the third component of complement using two-dimensional electrophoresis.
    Shimazaki Youji, Muro Masayoshi, Manabe Takashi, Jpn J Electroph., 45, (1) 89 - 92,   2001年
  • Isolation of the third component of complement and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster.
    Shimazaki Youji , Maeyama Kazutaka, Fujii Tamotsu, Developmental Comparative Immunology., 25, 467 - 474,   2001年
  • Two distinct mechanisms underlie the stimulation of neurotransmitter release by phorbol esters in clonal rat pheochromocytoma PC12 Cells.
    Iwasaki Satoshi, Kataoka Masakazu, SEkiguchi Mariko, Shimazaki Yoji, Sato Kazuki, Takahashi Masami, Journal Biochemistry, 128, 407 - 414,   2000年
  • Detection and identification of constitutive polypeptides of the third component of complement using microscale two-dimensional electrophoresis
    Shimazaki Youji, Fujiwara Mai, Manabe Takashi, Jpn J Electroph., 44, (1) 21 - 25,   2000年
  • Selection of an effective enzyme for digestion of non-denaturing proteins using microscale two-dimensional electrophoresis.
    Shimazaki Youji, Muro Masayuki, Manabe Takashi, Clinica Chimica Acta, 302, 221 - 224,   2000年
  • Protein spot recognition on the non-denaturing and denaturing two-dimensional electrophoresis patterns using in situ immunosubtraction via Protein A agarose and antibodies .
    SHIMAZAKI Y, OHARA S, MANABE T, Journal of biochemical and biophysical methods, 39, (3) 179 - 184,   1999年
  • Localization of a visual Gg protein in the photoreceptors of a polychaete, Perinereis brevicirris (Annelida).
    Miyako-Shimazaki Yumiko, Shimazaki Youji, Ohtsu Kohzoh, Yamamoto Masamichi, Cell Tissue Research, 296, 427 - 431,   1999年
  • Application of two-dimensional electrophoresis without denaturing agents to comparative study of vertebrate plasma proteins.
    Shimazaki Youji, Manabe Takashi, Japanese Journal of electrophoresis, 42, (1) 13 - 18,   1998年
  • Separation of chicken egg proteins using two-dimensional electrophoresis in the absence of denaturing agents.
    Shimazaki Youi, Manabe Takashi, Japanese Journal of electrophoresis, 42, (3) 155 - 159,   1998年
  • Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies.
    SHIMAZAKI Y, OHARA S, MANABE T, Journal of biochemical and biophysical methods., 37, (1/2) 1 - 4,   1998年
  • Effect of formoguanamine on structure and function of the compound eye of the butterfly, Papilio xuthus.
    Miyako-Shimazaki Yumiko, Shimazaki Youji, Eguchi Eisuke, Zoological Science, 14, (1) 29 - 35,   1997年
  • Phosphorylation of 25-KDa Synaptosome-associated protein.
    Shimazaki Youji, Nishiki Tei-ichi, Omori Akira, Sekiguchi Mariko, Kamata Yoichi, Kozaki Shunji, Takahashi Masami, The Journal of Biological Chemistry, 271, (24) 14548 - 14553,   1996年
  • Light-dependent metabolic pathway of 3-hydroxy retinoids in the eye of a butterfly, Papilio xuthus.
    Shimazaki Youji, Eguchi Eisuke, Journal of Comparative Physiology A, 176, 661 - 671,   1995年
  • Synthesis of 3-hydroxyretinal in the Cytosol of the butterfly compound eye.
    Shimazaki Youji, Eguchi Eisuke, Vision Research, 33, (2) 155 - 163,   1993年
  • Changes in rhabdom volumes of isolated compouned eyes of the crab, Hemigrapsus sanguineus-A study in correlation with a circadian rhythm.
    Eguchi Eisuke, Shimazaki Youji, Suzuki Tatsuo, Zoological Science, 8, 453 - 460,   1991年

MISC

  • 分離. 電気泳動
    島崎洋次、真鍋敬, ぶんせき(日本分析化学会), (6) 301 - 302,   2001年

競争的資金

教育活動情報

担当経験のある科目

  • 生命の不思議, 愛媛大学
  • 科学リテラシー, 愛媛大学
  • 分析化学, 愛媛大学
  • 化学, 愛媛大学
  • 生命科学実験, 愛媛大学
  • 化学実験(基礎), 愛媛大学


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