研究者総覧

田上 瑠美 (タノウエ ルミ)

  • 沿岸環境科学研究センター 助教
Last Updated :2021/01/05

研究者情報

学位

  • 博士(理学)(愛媛大学)

ホームページURL

科研費研究者番号

  • 60767226

ORCID ID

J-Global ID

プロフィール

  • 「環境分析化学」を専門とし、環境試料や生体試料中の微量汚染化学物質の新規分析法開発と化学物質汚染のモニタリングを基盤研究としている。発展研究として、水圏環境に残留する生理活性化学物質の水生生物への移行・残留性の解析とその予測手法の開発に取り組んでいる。

    柔軟で前向き、かつ思慮深い研究者をめざしており、先導的・先進的でありながら丁寧で着実な研究を展開したいと考えている。


    主な研究テーマ

    1. 野生水生生物に残留する生理活性物質: 汚染実態解明と影響評価

    2. 生理活性物質の魚類への移行・残留性: 物質種および生物種特異性の解析と要因の特定

    3. アジア途上国における化学物質汚染の実態解明と影響評価

    4. 内分泌かく乱化学物質のヒト尿中レベルと曝露量の推定

    5. 水圏環境に残留する生理活性物質の新規分析法の開発

    6. 生物試料に残留する生理活性物質の新規分析法の開発

    7. 生体試料中内分泌かく乱化学物質の新規分析法の開発

    8. 生体試料中甲状腺ホルモンの新規分析法の開発


    ORCID: https://orcid.org/0000-0003-2354-0694

    研究室 HP: http://kanka.cmes.ehime-u.ac.jp/

    研究室 FB: https://www.facebook.com/kankaatcmes/

    共同利用・共同研究拠点 LaMer: http://lamer-cmes.jp/

    CMES: http://www.cmes.ehime-u.ac.jp/

    es-BANK: http://esbank-ehime.com/dnn/Default.aspx?alias=esbank-ehime.com/dnn/j

研究キーワード

  • 環境汚染物質   環境汚染   LC-MS/MS   環境科学   環境化学   野生生物   魚類   取込と排泄   生物移行・残留性   生物種差   バイオモニタリング   ヒト健康影響   水環境   内分泌かく乱化学物質   体内動態   水生生物   医薬品類およびパーソナルケア製品由来化学物質   環境分析化学   

研究分野

  • 環境・農学 / 環境負荷、リスク評価管理
  • 環境・農学 / 環境影響評価
  • 環境・農学 / 化学物質影響
  • 環境・農学 / 放射線影響

経歴

  • 2019年04月 - 現在  Ehime UniversityCenter for Marine Environmental Studies (CMES)Assistant Professor
  • 2019年04月 - 現在  愛媛大学沿岸環境科学研究センター助教(特定教員)
  • 2016年04月 - 2019年03月  Ehime UniversityCenter for Marine Environmental Studies (CMES)Researcher
  • 2016年04月 - 2019年03月  愛媛大学沿岸環境科学研究センター特定研究員
  • 2015年04月 - 2016年03月  Japan Society for the Promotion of SciencePostdoctoral researcher
  • 2015年04月 - 2016年03月  日本学術振興会特別研究員(PD)
  • 2014年04月 - 2015年03月  日本学術振興会特別研究員(DC2)
  • 2012年04月 - 2014年03月  愛媛大学沿岸環境科学研究センター特別研究員

学歴

  • 2012年04月 - 2015年03月   愛媛大学大学院   理工学研究科   博士後期課程 環境機能科学専攻
  • 2010年04月 - 2012年03月   熊本県立大学大学院   環境共生学研究科   博士前期課程
  • 2006年04月 - 2010年03月   熊本県立大学   環境共生学部   環境共生学科 生態・環境資源学専攻

所属学協会

  • 日本水環境学会   日本環境化学会   

研究活動情報

論文

  • Guruge KS, Goswami P, Tanoue R, Nomiyama K, Wijesekara RGS, Dharmaratne TS
    The Science of the total environment 690 683 - 695 2019年07月 [査読有り]
  • Tanoue R, Margiotta-Casaluci L, Huerta B, Runnalls TJ, Eguchi A, Nomiyama K, Kunisue T, Tanabe S, Sumpter JP
    The Science of the total environment 664 915 - 926 2019年05月 [査読有り]
  • Jiahua Guo, Shohei Ito, Hoa Thanh Nguyen, Kimika Yamamoto, Rumi Tanoue, Tatsuya Kunisue, Hisato Iwata
    Toxicology and Applied Pharmacology 347 23 - 32 2018年05月 [査読有り]
     研究論文(学術雑誌) 
    Triclosan (TCS), a commonly used antimicrobial compound, has recently been detected in the eggs of wild avian species. Exposure to TCS in rodents is known to interfere with thyroid hormone (TH), disrupt immune responses and cause liver disease. However, no attempt has been made to clarify the effects of TCS in avian species. The aim of this study is therefore to evaluate the toxic effects of in ovo exposure to TCS and explore the molecular mechanism by transcriptome analysis in the embryonic liver of a model avian species, chicken (Gallus gallus). Embryos were treated with graded concentration of TCS (0.1, 1 and 10 μg/g egg) at Hamburger Hamilton Stage (HHS) 1 (1st day), followed by 20 days of incubation to HHS 46. At the administration of 10 μg TCS/g egg, embryo mortality increased from 20% in control to 37% accompanied with 8% attenuation in tarsus length. While liver somatic index (LSI) in TCS treatments was enhanced, statistical difference was only observed at the treatment of 0.1 μg TCS/g egg in females. The up-regulation of several crucial differentially expressed genes (DEGs) in transcriptome analysis suggested that TCS induced xenobiotic metabolism (e.g. CYP2C23a, CYP2C45 and CYP3A37 in males CYP2C45 in females) and activated the thyroid hormone receptor (THR) - mediated downstream signaling (e.g. THRSPB and DIO2 in males THRSPB in females). In females, TCS may further activate the lipogenesis signaling (e.g. ACSL5, ELOVL2) and repress the lipolysis signaling (e.g. ABHD5, ACAT2). A battery of enriched transcription factors in relation to these TCS-induced signaling and phenotypes were found, including activated SREBF1, PPARa, LXRa, and LXRb in males and activated GLI2 in females COUP-TFII was predicted to be suppressed in both genders. Finally, we developed adverse outcome pathways (AOPs) that provide insights into the molecular mechanisms underlying the alteration of phenotypes.
  • Rumi Tanoue, Imari Kume, Yasuo Yamamoto, Kohki Takaguchi, Kei Nomiyama, Shinsuke Tanabe, Tatsuya Kunisue
    Journal of Chromatography A 1539 30 - 40 2018年03月 [査読有り]
     研究論文(学術雑誌) 
    Thyroid hormones (THs), which mainly consist of 3, 3′ 5-triiodo-L-thyronine (T3) and L-thyroxine (T4), play a critical role in regulating biological processes such as growth and metabolism in various animal species. Thus, accurate measurement of T3 and T4, especially physiologically active free (protein-unbound) forms, in serum/plasma is needed for the evaluation of TH homeostasis. However, such high-precision determination of free THs is lacking for non-human species. The present study aimed to develop a highly sensitive and reliable liquid chromatography-tandem mass spectrometry (LC–MS/MS) method for the determination of six free THs in serum/plasma, which is applicable to not only humans but also non-human species. Two different physical separation steps, ultrafiltration (UF) and equilibrium dialysis (ED), were examined to obtain the free TH fraction. Several experimental conditions were carefully optimized and validated for UF or ED using the commercially available bovine serum. As a result, UF at 1100 × g and 37 °C for 30 min with a 30 kDa ultrafiltration device (Centrifree YM-30, Millipore) yielded excellent precision (CV: < 10%). The optimized ED step also yielded high precision (CV: < 10%) and the measurement values were approximately equal to those of UF, but at least 16 h were required to reach equilibrium. Thus, UF combined with LC–MS/MS was finally chosen, in terms of the time needed for the measurement. Acceptable accuracy (recovery: 70%–110%) and intra- and inter-day precision (CV: < 10% and < 12%, respectively) were obtained, when triplicate analyses in three different days were conducted using the bovine serum. The developed analytical method was successfully applied to the determination of free THs in serum/plasma samples of humans, cats, and dogs. Furthermore, comparison with free T4 concentrations measured by a common immunoassay method evidently indicated that the ultrafiltration-LC–MS/MS method developed in this study can increase the specificity and accuracy of TH measurement.
  • Rumi Tanoue, Luigi Margiotta-Casaluci, Belinda Huerta, Tamsin J. Runnalls, Kei Nomiyama, Tatsuya Kunisue, Shinsuke Tanabe, John P. Sumpter
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 51 21 12825 - 12835 2017年11月 [査読有り]
     研究論文(学術雑誌) 
    Recent species-extrapolation approaches to the prediction of the potential effects of pharmaceuticals present in the environment on wild fish are based on the assumption that pharmacokinetics and metabolism in humans and fish are comparable. To test this hypothesis, we exposed fathead minnows to the opiate pro-drug tramadol and examined uptake from the water into the blood and brain and the metabolism of the drug into its main metabolites. We found that plasma concentrations could be predicted reasonably accurately based on the lipophilicity of the drug once the pH of the water was taken into account. The concentrations of the drug and its main metabolites were higher in the brain than in the plasma, and the observed brain and plasma concentration ratios were within the range of values reported in mammalian species. This fish species was able to metabolize the pro-drug tramadol into the highly active metabolite O-desmethyl tramadol and the inactive metabolite N-desmethyl tramadol in a similar manner to that of mammals. However, we found that concentration ratios of O-desmethyl tramadol to tramadol were lower in the fish than values in most humans administered the drug. Our pharmacokinetic data of tramadol in fish help bridge the gap between widely available mammalian pharmacological data and potential effects on aquatic organisms and highlight the importance of understanding drug uptake and metabolism in fish to enable the full implementation of predictive toxicology approaches.
  • Rumi Tanoue, Kei Nomiyama, Haruna Nakamura, Joon-Woo Kim, Tomohiko Isobe, Ryota Shinohara, Tatsuya Kunisue, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 49 19 11649 - 11658 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    A fish plasma model (FPM) has been proposed as a screening technique to prioritize potential hazardous pharmaceuticals to wild fish. However, this approach does not account for inter- or intraspecies variability of pharmacokinetic and pharmacodynamic parameters. The present study elucidated the uptake potency (from ambient water), tissue distribution, and biological risk of 20 pharmaceutical and personal care product (PPCP) residues in wild cyprinoid fish inhabiting treated-wastewater-impacted streams. In order to clarify the uncertainty of the FPM for PPCPs, we compared the plasma bioaccumulation factor in the field (BAF(plasma) = measured fish plasma/ambient water concentration ratio) with the predicted plasma bioconcentration factor (BCFplasma = fish plasma predicted by use of theoretical partition coefficients/ambient water concentration ratio) in the actual environment. As a result, the measured maximum BAF(plasma) of inflammatory agents was up to 17 times higher than theoretical BCFplasma values, leading to possible underestimation of toxicological risk on wild fish. When the tissue blood partition coefficients (tissue/blood concentration ratios) of PPCPs were estimated, higher transportability into tissues, especially the brain, was found for psychotropic agents, but brain/plasma ratios widely varied among individual fish (up to 28-fold). In the present study, we provide a valuable data set on the intraspecies variability of PPCP pharmacokinetics, and our results emphasize the importance of determining PPCP concentrations in possible target organs as well as in the blood to assess the risk of PPCPs on wild fish.
  • Rumi Tanoue, Kei Nomiyama, Haruna Nakamura, Terutake Hayashi, Joon-Woo Kim, Tomohiko Isobe, Ryota Shinohara, Shinsuke Tanabe
    JOURNAL OF CHROMATOGRAPHY A 1355 193 - 205 2014年08月 [査読有り]
     研究論文(学術雑誌) 
    In the present study, a sensitive and accurate isotope dilution method was developed for the simultaneous determination of 17 polar pharmaceutical and personal care product (PPCP) residues (logK(ow) = 1.40-5.74), including 14 pharmaceuticals and 3 personal care products, in biological organs and tissues. The proposed method involved enzymatic hydrolysis, followed by sequential clean-up using silica gel chromatography and gel permeation chromatography, and analysis via ultra-high-performance liquid chromatography with tandem mass spectrometry. This method yielded acceptable absolute recoveries (48-88%) and internal standard-corrected recoveries (90-130%) for 17 PPCPs. Method detection limits were between 0.0092 and 3.2 ng g(-1) wet weight, and the limits of quantification were between 0.020 and 8.7 ng g(-1) wet weight. The method can be used to readily detect the target compounds at trace levels while minimizing the required sample volume. The developed method was applied to the determination of 17 PPCPs in the liver and kidney of 17 birds collected from Japan and also in the plasma, liver, and brain of 7 cyprinoid fish from an effluent-dominated stream in Japan. Triclosan was detected in 5 of 11 fish-eating birds but not in non-fish-eating birds, suggesting the contamination of prey fish by the chemical. Non-steroidal anti-inflammatory drugs, antibacterial agents, and psychotropic agents were frequently detected in the fish tissues. In addition, 7 of the target compounds were found in fish brain. The median brain/plasma ratios of the psychotropic agents ranged from 1.6 (carbamazepine) to 12 (diphenhydramine), indicating high transportability to fish brain. (C) 2014 Elsevier B.V. All rights reserved.
  • Rumi Tanoue, Yuri Sato, Mild Motoyama, Shuhei Nakagawa, Ryota Shinohara, Kei Nomiyama
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 41 10203 - 10211 2012年10月 [査読有り]
     研究論文(学術雑誌) 
    Land application of recycled manure produced from biosolids and reclaimed wastewater can transfer pharmaceutical chemicals to terrestrial environments, giving rise to potential accumulation of these residues in edible plants. In this study, the potential for plant uptake of 13 pharmaceutical chemicals, and the relation between the accumulation features within the plant and the physicochemical properties were examined by exposing pea and cucumber to an aqueous solution containing pharmaceutical chemicals. Ten of 13 compounds tested were detected in plant leaves and stems. Comparison of the plant uptake characteristics and the octanol water partition coefficient of pharmaceutical chemicals showed that compounds with an intermediate polarity such as carbamazepine and crotamiton could be easily transported to plant shoots. Moreover, these results suggest the possibility of highly hydrophilic pharmaceutical chemicals such as trimethoprim and sulfonamides to be accumulated in plant roots owing to their low permeability in root cell membranes.
  • Miki Motoyama, Shuhei Nakagawa, Rumi Tanoue, Yuri Sato, Kei Nomiyama, Ryota Shinohara
    CHEMOSPHERE 84 4 432 - 438 2011年07月 [査読有り]
     研究論文(学術雑誌) 
    In recent years, sludge generated in sewage treatment plants (STPs) and solid waste from livestock being utilized is useful for circulation of nourishment in farmlands as recycled organic manure (ROM). In this study, we determined the residue levels and patterns of 12 pharmaceutical products generated by human activity in the ROMs produced from human waste sludge (HWS), sewage sludge (SS), cattle manure (CM), poultry manure (PM), swine manure (SM) and horse manure (HM). The kind and number of pharmaceutical products detected in ROMs were different. Fluoroquinolones (FQs) were detected at high levels in HWS and SS samples. In addition, the detection frequency and concentration levels of sulfonamides (SAs) in PM and SM were high. Moreover, high concentrations of chlortetracycline (CTC) were found in only SM. These differences reflect specific adherence adsorption of the pharmaceutical products to different livestock and humans. Moreover, it was found that the concentrations of pharmaceutical products and fermentation levels of ROMs had significant positive correlation (r = 0.41, p = 0.024). When the fermentation test of ROM was conducted in a rotary fermentor in a lab scale test, the residue levels of pharmaceutical products decreased effectively except carbamazepine (CBZ). The rates of decrease were in the case of tetracyclines (TCs): 85-92%, FQs: 81-100%, erythromycine: 67%, SAs: 79-95%, trimethoprim: 86% and CBZ: 37% by 30 d. Pharmaceutical products that can be decomposed by fermentation process at the lowest impact of residual antibiotic activities may therefore be considered as environmentally friendly medicines. (C) 2011 Elsevier Ltd. All rights reserved.

MISC

  • Kohki Takaguchi, Hiroyuki Nishikawa, Hazuki Mizukawa, Rumi Tanoue, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shouta M M Nakayama, Yoshinori Ikenaka, Tatsuya Kunisue, Mayumi Ishizuka, Shinsuke Tanabe, Hisato Iwata, Kei Nomiyama The Science of the total environment 688 1172 -1183 2019年10月 
    Polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) might disrupt thyroid function. However, there is no clear evidence of PCB exposure disrupting thyroid hormone (TH) homeostasis in dogs and cats. The present study conducted in vivo experiments to evaluate the effects of a mixture of 12 PCB congeners (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187 and 202, each congener 0.5 mg/kg BW, i.p. administration) on serum TH levels in male dogs (Canis lupus familiaris) and male cats (Felis silvestris catus). In PCB-exposed dogs, the time courses of higher-chlorinated PCBs and L-thyroxine (T4)-like OH-PCBs (4-OH-CB107 and 4-OH-CB202) concentrations were unchanged or tended to increase, whereas those of lower-chlorinated PCBs and OH-PCBs tended to decrease after 24 h. In PCB-exposed cats, concentrations of PCBs increased until 6 h and then remained unchanged. The levels of lower-chlorinated OH-PCBs including 4'-OH-CB18 increased until 96 h and then decreased. In PCB-exposed dogs, free T4 concentrations were higher than those in the control group at 48 and 96 h after PCB administration and positively correlated with the levels of T4-like OH-PCBs, suggesting competitive binding of T4 and T4-like OH-PCBs to a TH transporter, transthyretin. Serum levels of total T4 and total 3,3',5-triiodo-L-thyronine (T3) in PCB-exposed dogs were lower than in the control group at 24 and 48 h and negatively correlated with PCB concentrations, implying that PCB exposure enhanced TH excretion by increasing TH uptake and TH conjugation enzyme activities in the dog liver. In contrast, no obvious changes in TH levels were observed in PCB-exposed cats. This could be explained by the lower levels of T4-like OH-PCBs and lower hepatic conjugation enzyme activities in cats compared with dogs. Different effects on serum TH levels in PCB-exposed dogs and cats are likely to be attributable to species-specific PCB and TH metabolism.

受賞

  • 2017年06月 第26回環境化学討論会 優秀発表賞(35歳以下の若手研究者の部)
     
    受賞者: 田上 瑠美
  • 2016年09月 平成28年度日本水環境学会 博士研究奨励賞(オルガノ賞)
     
    受賞者: 田上 瑠美
  • 2016年09月 第22回日本環境毒性学会研究発表会 若手研究奨励賞
     
    受賞者: 田上 瑠美
  • 2013年08月 第22回環境化学討論会 最優秀学生賞(博士後期課程の部)
     
    受賞者: 田上 瑠美
  • 2012年07月 第21回環境化学討論会 優秀学生賞(博士後期課程の部)
     
    受賞者: 田上 瑠美
  • 2011年07月 第20回環境化学討論会 優秀学生賞(博士前期課程の部)
     
    受賞者: 田上 瑠美

共同研究・競争的資金等の研究課題

  • 生理活性化学物質の魚類への移行/残留性の解析および生物濃縮性予測モデルの構築
    日本学術振興会:科学研究費補助金: 若手研究
    研究期間 : 2018年04月 -2020年03月 
    代表者 : 田上 瑠美
  • 下水処理水に含まれる生理活性化学物質の水生生物への移行/残留性の評価
    公益財団法人日本生命財団:平成29年度若手研究・奨励研究助成
    研究期間 : 2017年10月 -2018年09月 
    代表者 : 田上 瑠美
  • 魚類における解熱鎮痛消炎剤(NSAIDs)の体内挙動および影響発現との関連解析
    国立大学法人愛媛大学:共同利用・共同研究拠点「化学汚染・沿岸環境研究拠点」(LaMer) 若手国際共同研究
    研究期間 : 2017年04月 -2018年03月 
    代表者 : 田上 瑠美
  • 水環境中に残留する生活関連化学物質の魚類への影響評価 〜動態学的および動力学的機構の定量的解析〜
    公益財団法人日本科学協会:平成29年度笹川科学研究助成
    研究期間 : 2017年04月 -2018年02月 
    代表者 : 田上 瑠美
  • 水環境に残留する向精神剤の魚類への影響評価 ~動態学的および動力学的機構の定量的解析~
    国立大学法人愛媛大学:共同利用・共同研究拠点「化学汚染・沿岸環境研究拠点」(LaMer) 若手国際共同研究
    研究期間 : 2016年04月 -2017年03月 
    代表者 : 田上 瑠美
  • 野生生物における極性PPCPsの汚染実態および生体残留性の解明とリスク評価
    日本学術振興会:科学研究費補助金: 特別研究奨励費
    研究期間 : 2014年04月 -2016年03月 
    代表者 : 田上 瑠美

その他

  • 留学経験 
    2013年2月~2013年3月 米国Baylor Universityに留学(約1ヶ月) 2015年6月~2015年3月 英国Brunel Universityに留学(約9ヶ月)
  • 資格 
    2009年 5月 環境計量士(濃度関係)国家試験合格 2009年 11月 環境計量講習(濃度関係)修了 2009年 12月 公害防止管理者(水質関係)資格 2019年 2月 環境計量士(濃度関係)登録(登録番号:第11073号)


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