研究者データベース

仲山 慶ナカヤマ ケイ

所属部署名沿岸環境科学研究センター
職名助教
Last Updated :2020/05/30

研究者基本情報

基本情報

氏名

  • 氏名

    仲山 慶
  • 氏名(カナ)

    ナカヤマ ケイ

基本情報

所属

所属・職名

  • 部署

    沿岸環境科学研究センター 化学汚染・毒性解析部門 沿岸環境科学研究センター
  • 職名

    講師

学歴等

学位

  • 博士(農学)

その他基本情報

所属学協会

  • 日本水産学会
  • The Society of Environmental Toxicology and Chemistry
  • 日本環境毒性学会
  • The Society of Environmental Toxicology and Chemistry

経歴

  • 2014年 - , 愛媛大学 講師

研究活動情報

研究分野等

研究分野

  • 環境・農学, 化学物質影響
  • 環境・農学, 放射線影響
  • 環境・農学, 環境影響評価
  • ライフサイエンス, 水圏生産科学
  • ライフサイエンス, 水圏生産科学

研究キーワード

  • マイクロアレイ
  • 有害化学物質
  • 環境
  • 農林水産物
  • シトクロムP450
  • POPs候補物質
  • リスク評価
  • アジア途上地域
  • マボヤ
  • リンホシスチス病
  • カワウ
  • 熱ショックタンパク質
  • ダイオキシン類
  • 環境汚染
  • 鞭毛虫
  • 異物代謝酵素
  • リンホシスチスウイルス
  • 歴史トレンド
  • 有機フッ素化合物
  • 寄生虫
  • バイオインフォマティクス
  • トキシコゲノミクス
  • 内分泌かく乱
  • 環境毒性学
  • Bioinformatics
  • Toxicogenomics
  • Endocrine Disruption
  • Environmental Toxicology

著書・発表論文等

論文

  • Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection, Eitaro Sawayama, Shin-Ichi Kitamura, Kei Nakayama, Kohei Ohta, Hiroyuki Okamoto, Akiyuki Ozaki, Motohiro Takagi, Aquaculture, Aquaculture, 2019年04月, [査読有り]
  • Atlas of the telencephalon based on cytoarchitecture, neurochemical markers, and gene expressions in Rhinogobius flumineus [Mizuno, 1960]., Masahumi Kawaguchi, Hanako Hagio, Naoyuki Yamamoto, Koji Matsumoto, Kei Nakayama, Yasuhisa Akazome, Hironori Izumi, Yousuke Tsuneoka, Fumikazu Suto, Yasunori Murakami, Hiroyuki Ichijo, The Journal of comparative neurology, The Journal of comparative neurology, 2019年03月, [査読有り], 0021-9967, 10.1002/cne.24547
  • Measurement of Tunic Hardness in an Edible Ascidian, Halocynthia roretzi, with Remarks on Soft Tunic Syndrome., Hirose E, Nakayama K, Yanagida T, Nawata A, Kitamura SI, Zoological science, Zoological science, 2018年12月, [査読有り], 0289-0003, 10.2108/zs180072
  • Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge., Noman Reza MA, Mohapatra S, Shimizu S, Kitamura SI, Harakawa S, Kawakami H, Nakayama K, Sawayama E, Matsubara T, Ohta K, Chakraborty T, Fish & shellfish immunology, Fish & shellfish immunology, 2018年08月, [査読有り], 1050-4648, 10.1016/j.fsi.2018.08.027
  • Extracellular Proteinases of Miamiensis avidus Causing Scuticociliatosis are Potential Virulence Factors, Narasaki Yukie, Obayashi Yumiko, Ito Sayami, Murakami Shoko, Song Jun-Young, Nakayama Kei, Kitamura Shin-Ichi, FISH PATHOLOGY, FISH PATHOLOGY, 2018年03月, [査読有り], 0388-788X, 10.3147/jsfp.53.1
  • Identification, Characterization, and Mapping of a Novel SNP Associated with Body Color Transparency in Juvenile Red Sea Bream (Pagrus major)., Sawayama E, Noguchi D, Nakayama K, Takagi M, Marine biotechnology (New York, N.Y.), Marine biotechnology (New York, N.Y.), 2018年03月, [査読有り], 1436-2228, 10.1007/s10126-018-9810-z
  • Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major), Eitaro Sawayama, Shiho Tanizawa, Shin-Ichi Kitamura, Kei Nakayama, Kohei Ohta, Akiyuki Ozaki, Motohiro Takagi, MARINE BIOTECHNOLOGY, MARINE BIOTECHNOLOGY, 2017年12月, [査読有り], 1436-2228, 10.1007/s10126-017-9779-z
  • Tributyltin exposure increases mortality of nodavirus infected Japanese medaka Oryzias latipes larvae, Shin-Ichi Kitamura, Masaki Akizuki, Jun-Young Song, Kei Nakayama, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2017年11月, [査読有り], 0025-326X, 10.1016/j.marpolbul.2017.02.020
  • Nervous system disruption and swimming abnormality in early-hatched pufferfish (Takifugu niphobles) larvae caused by pyrene is independent of aryl hydrocarbon receptors, Tatsuya Itoyama, Moe Kawara, Makiko Fukui, Yuki Sugahara, Daisuke Kurokawa, Masahumi Kawaguchi, Shin-Ichi Kitamura, Kei Nakayama, Yasunori Murakami, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2017年11月, [査読有り], 0025-326X, 10.1016/j.marpolbul.2017.02.058
  • Use of common carp (Cyprinus carpio) and Aeromonas salmonicida for detection of immunomodulatory effects of chemicals on fish, Kei Nakayama, Ryohei Yamashita, Shin-Ichi Kitamura, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2017年11月, [査読有り], 0025-326X, 10.1016/j.marpolbul.2016.12.060
  • Analysis of genes encoding high-antigenicity polypeptides in three serotypes of Miamiensis avidus., Motokawa S, Narasaki Y, Song JY, Yokoyama Y, Hirose E, Murakami S, Jung SJ, Oh MJ, Nakayama K, Kitamura SI, Parasitology international, Parasitology international, 2017年10月, [査読有り], 1383-5769, 10.1016/j.parint.2017.10.007
  • Alteration of development and gene expression induced by in ovo-nanoinjection of 3-hydroxybenzo[c]phenanthrene into Japanese medaka (Oryzias latipes) embryos, Kun Chen, Yuki Tsutsumi, Shuhei Yoshitake, Xuchun Qiu, Hai Xu, Yasuyuki Hashiguchi, Masato Honda, Kosuke Tashiro, Kei Nakayama, Takeshi Hano, Nobuo Suzuki, Kazuichi Hayakawa, Yohei Shimasaki, Yuji Oshima, AQUATIC TOXICOLOGY, AQUATIC TOXICOLOGY, 2017年01月, [査読有り], 0166-445X, 10.1016/j.aquatox.2016.11.020
  • Cellular and Molecular Hypoxic Response in Common Carp (Cyprinus carpio) Exposed to Linear Alkylbenzene Sulfonate at Sublethal Concentrations, Junichi Tasaki, Kei Nakayama, Ichiyo Shimizu, Hiroko Yamada, Takahiro Suzuki, Naohiro Nishiyama, Masayuki Yamane, ENVIRONMENTAL TOXICOLOGY, ENVIRONMENTAL TOXICOLOGY, 2017年01月, [査読有り], 1520-4081, 10.1002/tox.22217
  • OCCURRENCE OF GLUCOCORTICOIDS DISCHARGED FROM A SEWAGE TREATMENT PLANT IN JAPAN AND THE EFFECTS OF CLOBETASOL PROPIONATE EXPOSURE ON THE IMMUNE RESPONSES OF COMMON CARP (CYPRINUS CARPIO) TO BACTERIAL INFECTION, Nakayama, Kei, Sato, Kentaro, Shibano, Takazumi, Isobe, Tomohiko, Suzuki, Go, Kitamura, Shin-Ichi, Environmental Toxicology and Chemistry, Environmental Toxicology and Chemistry, 2016年, [査読有り], 0730-7268, 10.1002/etc.3136
  • Cellulose is not degraded in the tunic of the edible ascidian Halocynthia roretzi contracting soft tunic syndrome., Kimura S, Nakayama K, Wada M, Kim UJ, Azumi K, Ojima T, Nozawa A, Kitamura S, Hirose E, Diseases of aquatic organisms, Diseases of aquatic organisms, 2015年10月, [査読有り], 0177-5103, 10.3354/dao02913
  • Determination of natural and synthetic glucocorticoids in effluent of sewage treatment plants using ultrahigh performance liquid chromatography-tandem mass spectrometry, Isobe, Tomohiko, Sato, Kentaro, Joon-Woo, Kim, Tanabe, Shinsuke, Suzuki, Go, Nakayama, Kei, Environmental Science and Pollution Research, Environmental Science and Pollution Research, 2015年, [査読有り], 0944-1344, 10.1007/s11356-015-4626-0
  • Detection of glucocorticoid receptor agonists in effluents from sewage treatment plants in Japan, Suzuki, Go, Sato, Kentaro, Isobe, Tomohiko, Takigami, Hidetaka, Brouwer, Abraham, Nakayama, Kei, Science of the Total Environment, Science of the Total Environment, 2015年, [査読有り], 0048-9697, 10.1016/j.scitotenv.2015.05.008
  • RNA Sequencing Revealed Numerous Polyketide Synthase Genes in the Harmful Dinoflagellate Karenia mikimotoi., Kimura K, Okuda S, Nakayama K, Shikata T, Takahashi F, Yamaguchi H, Skamoto S, Yamaguchi M, Tomaru Y, PloS one, PloS one, 2015年, [査読有り], 10.1371/journal.pone.0142731
  • Effects of persistent organochlorine exposure on the liver transcriptome of the common minke whale (Balaenoptera acutorostrata) from the North Pacific, Satoko Niimi, Mai Imoto, Tatsuya Kunisue, Michio X. Watanabe, Eun-Young Kim, Kei Nakayama, Genta Yasunaga, Yoshihiro Fujise, Shinsuke Tanabe, Hisato Iwata, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2014年10月, [査読有り], 0147-6513, 10.1016/j.ecoenv.2014.06.028
  • Uptake and biological effects of synthetic glucocorticoids in common carp (Cyprinus carpio), Kei Nakayama, Yoshiyuki Inoue, Naomi Ikeda, Naoki Hashizume, Hidekazu Murakami, Takeshi Ishibashi, Hirofumi Ikeda, Tomohiko Isobe, Shin-Ichi Kitamura, Go Suzuki, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2014年08月, [査読有り], 0025-326X, 10.1016/j.marpolbul.2014.01.042
  • Pyrene induces a reduction in midbrain size and abnormal swimming behavior in early-hatched pufferfish larvae, Yuki Sugahara, Masahumi Kawaguchi, Tatsuya Itoyama, Daisuke Kurokawa, Yasuhiko Tosa, Shin-Ichi Kitamura, Itsuki C. Handoh, Kei Nakayama, Yasunori Murakami, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2014年08月, [査読有り], 0025-326X, 10.1016/j.marpolbul.2014.04.022
  • Host responses of Japanese flounder Paralichthys olivaceus with lymphocystis cell formation, Shogo Iwakiri, Jun-Young Song, Kei Nakayama, Myung-Joo Oh, Minoru Ishida, Shin-Ichi Kitamura, FISH & SHELLFISH IMMUNOLOGY, FISH & SHELLFISH IMMUNOLOGY, 2014年06月, [査読有り], 1050-4648, 10.1016/j.fsi.2014.03.028
  • Nervous system disruption and concomitant behavioral abnormality in early hatched pufferfish larvae exposed to heavy oil, Masahumi Kawaguchi, Yuki Sugahara, Tomoe Watanabe, Kouta Irie, Minoru Ishida, Daisuke Kurokawa, Shin-Ichi Kitamura, Hiromi Takata, Itsuki C. Handoh, Kei Nakayama, Yasunori Murakami, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2012年08月, [査読有り], 0944-1344, 10.1007/s11356-012-0833-0
  • Gas chromatography-mass spectrometry for metabolite profiling of Japanese medaka (Oryzias latipes) juveniles exposed to malathion, Seiichi Uno, Aoi Shintoyo, Emiko Kokushi, Masataka Yamamoto, Kei Nakayama, Jiro Koyama, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2012年08月, [査読有り], 0944-1344, 10.1007/s11356-012-0834-z
  • A time-course study of immune response in Japanese flounder Paralichthys olivaceus exposed to heavy oil, Jun-Young Song, Sho Ohta, Kei Nakayama, Yasunori Murakami, Shin-Ichi Kitamura, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2012年07月, [査読有り], 0944-1344, 10.1007/s11356-012-0737-z
  • Effect of heavy oil exposure on antibacterial activity and expression of immune-related genes in Japanese flounder Paralichthys olivaceus, Jun-Young Song, Kei Nakayama, Emiko Kokushi, Kazuki Ito, Seiichi Uno, Jiro Koyama, M. Habibur Rahman, Yasunori Murakami, Shin-Ichi Kitamura, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2012年04月, [査読有り], 0730-7268, 10.1002/etc.1743
  • Integrative assessment of potential effects of dioxins and related compounds in wild Baikal seals (Pusa sibirica): Application of microarray and biochemical analyses, Shusaku Hirakawa, Daisuke Imaeda, Kei Nakayama, Masayuki Udaka, Eun-Young Kim, Tatsuya Kunisue, Masako Ogawa, Tomonari Matsuda, Saburo Matsui, Evgeny A. Petrov, Valeriy B. Batoev, Shinsuke Tanabe, Hisato Iwata, AQUATIC TOXICOLOGY, AQUATIC TOXICOLOGY, 2011年09月, [査読有り], 0166-445X, 10.1016/j.aquatox.2011.05.007
  • Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes, Mohamed Nassef, Yoko Kato-Unoki, Tomohisa Furuta, Kei Nakayama, Hina Satone, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, ZOOLOGICAL SCIENCE, ZOOLOGICAL SCIENCE, 2011年04月, [査読有り], 0289-0003, 10.2108/zsj.28.281
  • Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes), Kei Nakayama, Naomi Sei, Itsuki C. Handoh, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2011年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2011.01.015
  • Heavy oil exposure induces high moralities in virus carrier Japanese flounder Paralichthys olivaceus, Jun-Young Song, Kei Nakayama, Yasunori Murakami, Shin-Ichi Kitamura, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2011年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2011.01.020
  • Contamination status of POPs and BFRs and relationship with parasitic infection in finless porpoises (Neophocaena phocaenoides) from Seto Inland Sea and Omura Bay, Japan, Tomohiko Isobe, Tomoko Oshihoi, Hiroki Hamada, Kei Nakayama, Tadasu K. Yamada, Yuko Tajima, Masao Amano, Shinsuke Tanabe, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2011年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2011.01.014
  • Disruption of Sema3A expression causes abnormal neural projection in heavy oil exposed Japanese flounder larvae, Masahumi Kawaguchi, Jun-Young Song, Kouta Irie, Yasunori Murakami, Kei Nakayama, Shin-Ichi Kitamura, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2011年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2011.01.022
  • Effect of heavy oil on the development of the nervous system of floating and sinking teleost eggs, Kouta Irie, Masahumi Kawaguchi, Kaori Mizuno, Jun-Young Song, Kei Nakayama, Shin-Ichi Kitamura, Yasunori Murakami, MARINE POLLUTION BULLETIN, MARINE POLLUTION BULLETIN, 2011年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2011.04.018
  • Does heavy oil pollution induce bacterial diseases in Japanese flounder Paralichthys olivaceus?, Song JY, Nakayama K, Murakami Y, Jung SJ, Oh MJ, Matsuoka S, Kawakami H, Kitamura S, Marine pollution bulletin, Marine pollution bulletin, 2008年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2008.01.024
  • Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126., Nakayama K, Sei N, Oshima Y, Tashiro K, Shimasaki Y, Honjo T, Marine pollution bulletin, Marine pollution bulletin, 2008年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2008.02.020
  • Toxicogenomic analysis of immune system-related genes in Japanese flounder (Paralichthys olivaceus) exposed to heavy oil., Nakayama K, Kitamura S, Murakami Y, Song JY, Jung SJ, Oh MJ, Iwata H, Tanabe S, Marine pollution bulletin, Marine pollution bulletin, 2008年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2008.02.021
  • Effects of heavy oil in the developing spotted halibut, Verasper variegatus., Murakami Y, Kitamura S, Nakayama K, Matsuoka S, Sakaguchi H, Marine pollution bulletin, Marine pollution bulletin, 2008年, [査読有り], 0025-326X, 10.1016/j.marpolbul.2008.02.043
  • Effect of tributyltin on reproduction in Japanese whiting, Sillago japonica, Yohei Shimasaki, Yuji Oshima, Suguru Inoue, Yoshiyuki Inoue, Ik Joon Kang, Kei Nakayama, Hisaya Imoto, Tsuneo Honjo, MARINE ENVIRONMENTAL RESEARCH, MARINE ENVIRONMENTAL RESEARCH, 2006年, [査読有り], 0141-1136, 10.1016/j.marenvres.2006.04.030
  • Effects of polychlorinated biphenyls on the schooling behavior of Japanese medaka (Oryzias latipes), K Nakayama, Y Oshima, K Hiramatsu, Y Shimasaki, T Honjo, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2005年10月, [査読有り], 0730-7268, 10.1897/04-518R2.1
  • Suppression of sexual behavior in male Japanese medaka (Oryzias latipes) exposed to 17 beta-estradiol, Y Oshima, IJ Kang, M Kobayashi, K Nakayama, N Imada, T Honjo, CHEMOSPHERE, CHEMOSPHERE, 2003年01月, [査読有り], 0045-6535, 10.1016/S0045-6535(02)00494-0

書籍等出版物

MISC

  • ハゼ科魚類ヨシノボリの終脳に見いだされた基底核由来の複雑な神経核に関する解析, 川口将史, 山本直之, 松本浩司, 仲山慶, 萩尾華子, 赤染康久, 和泉宏謙, 恒岡洋右, 須藤文和, 村上安則, 一條裕之, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 123rd, 155, 2018年, http://jglobal.jst.go.jp/public/201802249137762704
  • 愛媛県松山市重信川水系および西条市渦井川水系のコイを対象にしたコイヘルペスウイルスの感染実態調査, 志水将人, 仲山慶, 畑啓生, 北村真一, 今城雅之, 日本水産学会大会講演要旨集, 日本水産学会大会講演要旨集, 2017, 147, 2017年03月, http://jglobal.jst.go.jp/public/201702220809398260
  • ヨシノボリ属の求愛行動を制御する神経基盤の解析, 川口将史, 柴田淳也, 川西亮太, 曽我部篤, 仲山慶, 山本直之, 大森浩二, 須藤文和, 一條裕之, 松本浩司, 村上安則, 日本動物学会大会予稿集, 日本動物学会大会予稿集, 85th, 127, 2014年08月, http://jglobal.jst.go.jp/public/201402293433118935
  • E 環境・衛生 合成ステロイド剤の環境影響に対する懸念, 仲山慶, ファルマシア, ファルマシア, 47, 12, 1166, 1167, 2011年12月, 0014-8601, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102205616262468
  • Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes, Mohamed Nassef, Yoko Kato-Unoki, Tomohisa Furuta, Kei Nakayama, Hina Satone, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, ZOOLOGICAL SCIENCE, ZOOLOGICAL SOC JAPAN, ZOOLOGICAL SCIENCE, 28, 4, 281, 285, 2011年04月, 0289-0003, 10.2108/zsj.28.281, The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat. TBT-bp1 revealed that the exon-intron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat. TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription - PCR revealed that Olat. TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat. TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat. TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat. TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.
  • Molecular Cloning, Sequencing, and Gene Expression Analysis of Tributyltin-Binding Protein Type 1 in Japanese Medaka Fish, Oryzias latipes, Mohamed Nassef, Yoko Kato-Unoki, Tomohisa Furuta, Kei Nakayama, Hina Satone, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, ZOOLOGICAL SCIENCE, ZOOLOGICAL SOC JAPAN, ZOOLOGICAL SCIENCE, 28, 4, 281, 285, 2011年04月, 0289-0003, 10.2108/zsj.28.281, The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat. TBT-bp1 revealed that the exon-intron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat. TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription - PCR revealed that Olat. TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat. TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat. TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat. TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.
  • Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27, Mohamed Nassef, Takahiko Tawaratsumita, Yumi Oba, Hina Satone, Kei Nakayama, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, MARINE POLLUTION BULLETIN, PERGAMON-ELSEVIER SCIENCE LTD, MARINE POLLUTION BULLETIN, 62, 2, 412, 414, 2011年02月, 0025-326X, 10.1016/j.marpolbul.2010.12.005, In this study, individual Japanese flounder were intraperitoneally injected with 2 mu g tributyltin-d27 (TBT-d27) fish(-1). Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology. (C) 2010 Elsevier Ltd. All rights reserved.
  • Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27, Mohamed Nassef, Takahiko Tawaratsumita, Yumi Oba, Hina Satone, Kei Nakayama, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, MARINE POLLUTION BULLETIN, PERGAMON-ELSEVIER SCIENCE LTD, MARINE POLLUTION BULLETIN, 62, 2, 412, 414, 2011年02月, 0025-326X, 10.1016/j.marpolbul.2010.12.005, In this study, individual Japanese flounder were intraperitoneally injected with 2 mu g tributyltin-d27 (TBT-d27) fish(-1). Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology. (C) 2010 Elsevier Ltd. All rights reserved.
  • Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes), Kei Nakayama, Naomi Sei, Itsuki C. Handoh, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, MARINE POLLUTION BULLETIN, PERGAMON-ELSEVIER SCIENCE LTD, MARINE POLLUTION BULLETIN, 63, 5-12, 366, 369, 2011年, 0025-326X, 10.1016/j.marpolbul.2011.01.015, The effects of polychlorinated biphenyls (PCBs) on liver function and their differences between sexes were analyzed in Japanese medaka (Oryzias latipes) exposed to PCB126 or Kanechlor-400 (KC-400) using microarray. PCB exposure induced vitellogenin 1 expression in female medaka while suppressing choriogenin genes, which suggests that the effects of PCBs on estrogen-responsive genes do not occur directly through an estrogen receptor-mediated pathway. Reduction of androgen receptor alpha expression was also observed, and the gene expression pattern in PCB-exposed males changed to become more similar to that of females. Furthermore, changes in glycolysis-related genes indicate that PCB exposure might enhance glucose production via gluconeogenesis in the liver of medaka. Taken together, our results suggest that PCBs disrupt the endocrine system, especially androgen function, and may have the potential to cause demasculinizing effects. Additionally, induction of gluconeogenesis might be a response to maintain glucose levels consumed as a result of PCB exposures. (C) 2011 Elsevier Ltd. All rights reserved.
  • Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes), Kei Nakayama, Naomi Sei, Itsuki C. Handoh, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, MARINE POLLUTION BULLETIN, PERGAMON-ELSEVIER SCIENCE LTD, MARINE POLLUTION BULLETIN, 63, 5-12, 366, 369, 2011年, 0025-326X, 10.1016/j.marpolbul.2011.01.015, The effects of polychlorinated biphenyls (PCBs) on liver function and their differences between sexes were analyzed in Japanese medaka (Oryzias latipes) exposed to PCB126 or Kanechlor-400 (KC-400) using microarray. PCB exposure induced vitellogenin 1 expression in female medaka while suppressing choriogenin genes, which suggests that the effects of PCBs on estrogen-responsive genes do not occur directly through an estrogen receptor-mediated pathway. Reduction of androgen receptor alpha expression was also observed, and the gene expression pattern in PCB-exposed males changed to become more similar to that of females. Furthermore, changes in glycolysis-related genes indicate that PCB exposure might enhance glucose production via gluconeogenesis in the liver of medaka. Taken together, our results suggest that PCBs disrupt the endocrine system, especially androgen function, and may have the potential to cause demasculinizing effects. Additionally, induction of gluconeogenesis might be a response to maintain glucose levels consumed as a result of PCB exposures. (C) 2011 Elsevier Ltd. All rights reserved.
  • A microarray data analysis method to evaluate the impact of contaminants on wild animals, Kei Nakayama, Itsuki C. Handoh, Shin-Ichi Kitamura, Eun-Young Kim, Hisato Iwata, Shinsuke Tanabe, SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER SCIENCE BV, SCIENCE OF THE TOTAL ENVIRONMENT, 408, 23, 5824, 5827, 2010年11月, 0048-9697, 10.1016/j.scitotenv.2010.08.065, Here we propose a novel microarray data analysis method applicable to evaluation of the chemical effects on wild animals First, we analyzed correlations between log-transformed hepatic 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent (TEQ) levels and probe signals detected in wild cormorant liver to screen contaminant-responsive genes Second, principal component analysis (PCA) was conducted using the screened probes Third, these probes were divided into two groups based on our PCA result Finally, we calculated Euclidian distance of signals, which is equivalent to variance of gene expressions, in each probe set, and analyzed the relationship between log-transformed hepatic TEQ levels and Euclidian distances A probe set whereby the calculated Euclidian distance was positively correlated with TEQ levels, could indicate genes that were directly affected by dioxins or other persistent organic pollutants (POPs), hence they can be used as biomarkers By contrast, there were a number of probes whereby the Euclidian distance was negatively correlated with TEQ levels In the latter probe group, the smaller Euclidian distances in highly contaminated individuals could point to changes in physiological activities of wild cormorants Therefore, our microarray data analysis method will provide new insights into POPs-responsive genes in field-collected samples for toxicogenomics studies (C) 2010 Elsevier BV All rights reserved
  • A microarray data analysis method to evaluate the impact of contaminants on wild animals, Kei Nakayama, Itsuki C. Handoh, Shin-Ichi Kitamura, Eun-Young Kim, Hisato Iwata, Shinsuke Tanabe, SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER SCIENCE BV, SCIENCE OF THE TOTAL ENVIRONMENT, 408, 23, 5824, 5827, 2010年11月, 0048-9697, 10.1016/j.scitotenv.2010.08.065, Here we propose a novel microarray data analysis method applicable to evaluation of the chemical effects on wild animals First, we analyzed correlations between log-transformed hepatic 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent (TEQ) levels and probe signals detected in wild cormorant liver to screen contaminant-responsive genes Second, principal component analysis (PCA) was conducted using the screened probes Third, these probes were divided into two groups based on our PCA result Finally, we calculated Euclidian distance of signals, which is equivalent to variance of gene expressions, in each probe set, and analyzed the relationship between log-transformed hepatic TEQ levels and Euclidian distances A probe set whereby the calculated Euclidian distance was positively correlated with TEQ levels, could indicate genes that were directly affected by dioxins or other persistent organic pollutants (POPs), hence they can be used as biomarkers By contrast, there were a number of probes whereby the Euclidian distance was negatively correlated with TEQ levels In the latter probe group, the smaller Euclidian distances in highly contaminated individuals could point to changes in physiological activities of wild cormorants Therefore, our microarray data analysis method will provide new insights into POPs-responsive genes in field-collected samples for toxicogenomics studies (C) 2010 Elsevier BV All rights reserved
  • Temporal and spatial trends of organotin contamination in the livers of finless porpoises (Neophocaena phocaenoides) and their association with parasitic infection status, Kei Nakayama, Chiho Matsudaira, Yuko Tajima, Tadasu K. Yamada, Motoi Yoshioka, Tomohiko Isobe, Shin Takahashi, Shinsuke Tanabe, SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER SCIENCE BV, SCIENCE OF THE TOTAL ENVIRONMENT, 407, 24, 6173, 6178, 2009年12月, 0048-9697, 10.1016/j.scitotenv.2009.08.043, Organotins (OTs) are globally ubiquitous contaminants. Contamination of OTs, particularly butyltins (BTs). has been suspected to Cause immunosuppressive effects leading to subsequent infectious diseases or opportunistic infection by pathogens in marine mammals. In the present study, we collected individuals from five different populations of finless porpoises (Neophocaena phocaenoides) from coastal areas of Japan (Seto Inland Sea, Ise Bay, Omura Bay, and Pacific coast) and Hong Kong. and measured OT concentrations in their livers. In addition, infection status of lung nematodes and liver trematodes was also observed to analyze the relationship between OT contamination and parasitic infection in finless porpoises. Hepatic OT concentrations were highest in the Hong Kong population, while levels in the Japanese populations were equivalent to those of other cetaceans from different locations. OT levels in Japan have been decreasing in the past few decades. while OT contamination has shown little improvement in Hong Kong. Observation of parasite infection in Seto Inland Sea porpoises revealed that 16 and 11 out of 22 individuals were infected by lung nematodes and liver trematodes, respectively. Additionally, a significant association between BT levels and parasitic infection status of lung nematodes was detected. Thus, the present study suggests that BTs could be a factor affecting parasitic infection. especially the presence or severity of lung nematodes in finless porpoises. Since chemical exposure may alter the susceptibility of organisms to infectious diseases. the interaction of chemical contamination with infectious diseases needs to be investigated in greater depth to understand the risk of population decline due to these factors in marine mammals. (C) 2009 Elsevier B.V. All rights reserved.
  • Temporal and spatial trends of organotin contamination in the livers of finless porpoises (Neophocaena phocaenoides) and their association with parasitic infection status, Kei Nakayama, Chiho Matsudaira, Yuko Tajima, Tadasu K. Yamada, Motoi Yoshioka, Tomohiko Isobe, Shin Takahashi, Shinsuke Tanabe, SCIENCE OF THE TOTAL ENVIRONMENT, ELSEVIER SCIENCE BV, SCIENCE OF THE TOTAL ENVIRONMENT, 407, 24, 6173, 6178, 2009年12月, 0048-9697, 10.1016/j.scitotenv.2009.08.043, Organotins (OTs) are globally ubiquitous contaminants. Contamination of OTs, particularly butyltins (BTs). has been suspected to Cause immunosuppressive effects leading to subsequent infectious diseases or opportunistic infection by pathogens in marine mammals. In the present study, we collected individuals from five different populations of finless porpoises (Neophocaena phocaenoides) from coastal areas of Japan (Seto Inland Sea, Ise Bay, Omura Bay, and Pacific coast) and Hong Kong. and measured OT concentrations in their livers. In addition, infection status of lung nematodes and liver trematodes was also observed to analyze the relationship between OT contamination and parasitic infection in finless porpoises. Hepatic OT concentrations were highest in the Hong Kong population, while levels in the Japanese populations were equivalent to those of other cetaceans from different locations. OT levels in Japan have been decreasing in the past few decades. while OT contamination has shown little improvement in Hong Kong. Observation of parasite infection in Seto Inland Sea porpoises revealed that 16 and 11 out of 22 individuals were infected by lung nematodes and liver trematodes, respectively. Additionally, a significant association between BT levels and parasitic infection status of lung nematodes was detected. Thus, the present study suggests that BTs could be a factor affecting parasitic infection. especially the presence or severity of lung nematodes in finless porpoises. Since chemical exposure may alter the susceptibility of organisms to infectious diseases. the interaction of chemical contamination with infectious diseases needs to be investigated in greater depth to understand the risk of population decline due to these factors in marine mammals. (C) 2009 Elsevier B.V. All rights reserved.
  • Antigenic differences of the scuticociliate Miamiensis avidus from Japan, J. Fish Dis., J. Fish Dis., 32, 1027, 1034, 2009年, 10.1111/j.1365-2761.2009.01089.x
  • Antigenic differences of the scuticociliate Miamiensis avidus from Japan, J. Fish Dis., J. Fish Dis., 32, 1027, 1034, 2009年, 10.1111/j.1365-2761.2009.01089.x
  • カワウ野生個体と培養細胞における遺伝子発現プロファイルの比較, 仲山 慶, 酒井 大樹, 北村 真一, 須藤 明子, 金 恩英, 田辺 信介, 岩田 久人, 環境毒性学会誌, 環境毒性学会誌, 11, 2, 75, 81, 2008年12月, 1344-0667, 10.11403/jset.11.75, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902295465904285
  • トキシコゲノミクスのフィールド調査への応用と問題点, 仲山慶, Endocr Disrupter News Lett, Endocr Disrupter News Lett, 11, 1, 5, 2008年06月, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902238516817613
  • 粉砕脱水法による魚肉からのCo‐PCBsの除去, 大嶋雄治, 堀就英, 井上英, 橋本伸哉, 仲山慶, 島崎洋平, 甲斐徳久, 本城凡夫, 九州大学大学院農学研究院学芸雑誌, 九州大学大学院農学研究院学芸雑誌, 63, 1, 41, 45, 2008年02月, 1347-0159, 10.15017/9849, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902204734578076
  • Potential effects of perfluorinated compounds in common cormorants from Lake Biwa, Japan: an implication from the hepatic gene expression profiles by microarray, NAKAYAMA Kei, IWATA Hisato, TAO Lin, KANNAN Kurunthachalam, IMOTO Mai, KIM Eun‐Young, TASHIRO Kosuke, TANABE Shinsuke, Environmental Toxicology and Chemistry, Environmental Toxicology and Chemistry, 27, 11, 2378, 2386, 2008年, 0730-7268, 10.1897/07-614.1
  • Real time PCR array to study effects of chemicals on the hypothalamic-pituitary-gonadal axis of the Japanese medaka, ZHANG Xiaowei, ZHANG Xiaowei, HECKER Markus, HECKER Markus, HECKER Markus, PARK June-woo, PARK June-woo, TOMPSETT Amber R, TOMPSETT Amber R, NEWSTED John, NEWSTED John, NAKAYAMA Kei, JONES Paul D, JONES Paul D, AU Doris, KONG Richard, WU Rudolf S.S, GIESY John P, GIESY John P, GIESY John P, GIESY John P, GIESY John P, Aquatic Toxicology, Aquatic Toxicology, 88, 3, 173, 182, 2008年, 0166-445X, 10.1016/j.aquatox.2008.04.009
  • Effects of heavy oil in the developing spotted halibut, <i>Verasper variegates</i>, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 524, 528, 2008年, 10.1016/j.marpolbul.2008.02.043
  • Does heavy oil pollution induce bacterial diseases in Japanese flounder <i>Paralichthys olivaceus</i> ?, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 889, 894, 2008年, 10.1016/j.marpolbul.2008.01.024
  • Toxicogenomic analysis of immune system-related genes in Japanese flounder (<i>Paralichthys olivaceus</i>) exposed to heavy oil, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 445, 452, 2008年, 10.1016/j.marpolbul.2008.02.021
  • Adverse effects of tributyltin on reproduction of Japanese medaka, <i>Oryzias latipes</i>, Coastal Marine Science, Coastal Marine Science, 32, 67, 76, 2008年
  • Alteration of gene expression profiles in the brain of Japanese medaka (<i>Oryzias latipes</i>) exposed to KC-400 or PCB126, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 460, 466, 2008年, 10.1016/j.marpolbul.2008.02.020
  • 粉砕脱水法による魚肉からのCo-PCBsの除去, 九州大学農学部学芸雑誌, 九州大学農学部学芸雑誌, 63, 41, 45, 2008年, 10.15017/9849
  • Distribution of tributyltin in tissues of mature Japanese whiting, <i>Sillago japonica</i> and their eggs, Journal of Faculty of Agriculture, Kyushu University, Journal of Faculty of Agriculture, Kyushu University, 53, 67, 72, 2008年
  • Potential effects of perfluorinated compounds in common cormorants from Lake Biwa, Japan: an implication from the hepatic gene expression profiles by microarray, Environmental Toxicology and Chemistry, Environmental Toxicology and Chemistry, 27, 11, 2378, 2386, 2008年, 0730-7268, 10.1897/07-614.1
  • Real time PCR array to study effects of chemicals on the hypothalamic-pituitary-gonadal axis of the Japanese medaka, Aquatic Toxicology, Aquatic Toxicology, 88, 3, 173, 182, 2008年, 0166-445X, 10.1016/j.aquatox.2008.04.009
  • Effects of heavy oil in the developing spotted halibut, <i>Verasper variegates</i>, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 524, 528, 2008年, 10.1016/j.marpolbul.2008.02.043
  • Does heavy oil pollution induce bacterial diseases in Japanese flounder <i>Paralichthys olivaceus</i> ?, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 889, 894, 2008年, 10.1016/j.marpolbul.2008.01.024
  • Toxicogenomic analysis of immune system-related genes in Japanese flounder (<i>Paralichthys olivaceus</i>) exposed to heavy oil, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 445, 452, 2008年, 10.1016/j.marpolbul.2008.02.021
  • Adverse effects of tributyltin on reproduction of Japanese medaka, <i>Oryzias latipes</i>, Coastal Marine Science, Coastal Marine Science, 32, 67, 76, 2008年
  • Alteration of gene expression profiles in the brain of Japanese medaka (<i>Oryzias latipes</i>) exposed to KC-400 or PCB126, Marine Pollution Bulletin, Marine Pollution Bulletin, 57, 460, 466, 2008年, 10.1016/j.marpolbul.2008.02.020
  • Distribution of tributyltin in tissues of mature Japanese whiting, <i>Sillago japonica</i> and their eggs, Journal of Faculty of Agriculture, Kyushu University, Journal of Faculty of Agriculture, Kyushu University, 53, 67, 72, 2008年
  • Alteration of monoamine concentrations in the brain of medaka, Oryzias latipes, exposed to tributyltin, Kei Nakayama, Yuji Oshima, Tetsuya Tachibana, Mitsuhiro Furuse, Tsuneo Honjo, ENVIRONMENTAL TOXICOLOGY, JOHN WILEY & SONS INC, ENVIRONMENTAL TOXICOLOGY, 22, 1, 53, 57, 2007年02月, 1520-4081, 10.1002/tox.20233, We measured the concentrations of monoamines in the brain of Japanese medaka, Oryzias latipes, exposed to tributyltin (TBT). Fish were exposed to 0, 1, 5, 25, or 125 mu g g(-1) of TBT via the diet for 21 days. After the administration period, six males and six females in each treatment group were dissected and their brains were collected. The following monoamines were analyzed: dopamine (DA), norepinephrine (NE), epinephrine (E), and 5-hydroxytryptamine (5-HT). The metabolites of DA, 3,4-dihydroxyphenylacetic acid and homovanilic acid, and the metabolite of 5-HT, 5-hydroxyindolacetic acid were also analyzed. The concentration of DA in the brain of male medaka and the concentrations of 5-HT and NE in the female brains were significantly decreased by exposure to 125 mu g TBT g(-1). The concentrations of 5-HT and NE in males and of DA in females were slightly decreased by 125 mu g g(-1) of TBT, although the differences were not statistically significant. The present study demonstrates that TBT alters monoamine concentrations in the brain of medaka. (c) 2007 Wiley Periodicals, Inc.
  • Alteration of monoamine concentrations in the brain of medaka, Oryzias latipes, exposed to tributyltin, Kei Nakayama, Yuji Oshima, Tetsuya Tachibana, Mitsuhiro Furuse, Tsuneo Honjo, ENVIRONMENTAL TOXICOLOGY, JOHN WILEY & SONS INC, ENVIRONMENTAL TOXICOLOGY, 22, 1, 53, 57, 2007年02月, 1520-4081, 10.1002/tox.20233, We measured the concentrations of monoamines in the brain of Japanese medaka, Oryzias latipes, exposed to tributyltin (TBT). Fish were exposed to 0, 1, 5, 25, or 125 mu g g(-1) of TBT via the diet for 21 days. After the administration period, six males and six females in each treatment group were dissected and their brains were collected. The following monoamines were analyzed: dopamine (DA), norepinephrine (NE), epinephrine (E), and 5-hydroxytryptamine (5-HT). The metabolites of DA, 3,4-dihydroxyphenylacetic acid and homovanilic acid, and the metabolite of 5-HT, 5-hydroxyindolacetic acid were also analyzed. The concentration of DA in the brain of male medaka and the concentrations of 5-HT and NE in the female brains were significantly decreased by exposure to 125 mu g TBT g(-1). The concentrations of 5-HT and NE in males and of DA in females were slightly decreased by 125 mu g g(-1) of TBT, although the differences were not statistically significant. The present study demonstrates that TBT alters monoamine concentrations in the brain of medaka. (c) 2007 Wiley Periodicals, Inc.
  • GENE EXPRESSION PROFILE IN THE LIVER OF BAIKAL SEALS (PUSA SIBIRICA): ASSOCIATION WITH DIOXINS AND RELATED COMPOUNDS, Hirakawa S, Imaeda D, Nakayama K, Kim EY, Kunisue T, Tanabe S, Petrov EA, Batoev VB, Iwata H, Organohalogen Compounds, Organohalogen Compounds, 69, 1741, 1744, 2007年
  • トキシコゲノミクスによるPOPs等有害化学物質の生体影響評価, 仲山 慶, 環境毒性学会誌, 環境毒性学会誌, 9, 2, 81, 86, 2006年12月, 1344-0667, 10.11403/jset.9.81, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902258349329270
  • Gene expression profiling in common cormorant liver with an oligo array: Assessing the potential toxic effects of environmental contaminants, K Nakayama, H Iwata, EY Kim, K Tashiro, S Tanabe, ENVIRONMENTAL SCIENCE & TECHNOLOGY, AMER CHEMICAL SOC, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 40, 3, 1076, 1083, 2006年02月, 0013-936X, 10.1021/es051386m, To establish a monitoring system for gene expression profiles related to chemical contamination in wild common cormorants (Phalacrocorax carbo), the present study constructed an oligo array designed from expressed sequence tag (EST) sequences of the cormorant liver, where 1061 unique oligonucleotides were spotted. Common cormorants were collected from Lake Biwa, Japan in May 2001 and 2002. With the use of this oligo array, gene expression profiles in the liver of individual specimens were evaluated. To determine the expression patterns of genes altered by environmental contaminants, relationships between concentrations of persistent organochlorines including polychlorinated dibenzo-p-dioxins, furans, polychlorinated biphenyls, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane and its metabolites (DOTS), hexachlorocyclohexane isomers (HCHs), chlordane compounds (CHLs), butyltins, and bisphenol A (BPA) and expression levels of each gene in the cormorant liver were examined using stepwise multiple regression analysis. The reliability of data obtained by the oligo array was further confirmed by quantifying the expression levels of certain genes using real-time RTPCR. The 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent (TEQ) level was positively correlated with both cytochrome P4501A4 and 1A5 gene expression. In addition, the mRNA level of an antioxidant enzyme, Cu/Zn superoxide dismutase, was negatively correlated with hepatic total TEQ. Other antioxidant enzymes, glutathione peroxidase 3 and glutathione S-transferase class mu, were negatively correlated with HCHs and BPA levels, respectively. The mRNA expression level of a nonenzymatic antioxidant, haptoglobin, was negatively but not significantly correlated with CHLs. These results led to a hypothesis that wild cormorant population may suffer from oxidative stress due to chemically induced formation of reactive oxygen species and subsequent reduction of antioxidant resistance. Thus, the cormorant oligo array may be a useful monitoring tool to identify specific gene expression profiles altered by various environmental contaminants. Although further research is required to clarify a definitive cause-and-effect relationship, the current study provides valuable information on contaminant-responsive genes to predict potential effects on wildlife in a real environment.
  • Gene expression profiling in common cormorant liver with an oligo array: Assessing the potential toxic effects of environmental contaminants, K Nakayama, H Iwata, EY Kim, K Tashiro, S Tanabe, ENVIRONMENTAL SCIENCE & TECHNOLOGY, AMER CHEMICAL SOC, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 40, 3, 1076, 1083, 2006年02月, 0013-936X, 10.1021/es051386m, To establish a monitoring system for gene expression profiles related to chemical contamination in wild common cormorants (Phalacrocorax carbo), the present study constructed an oligo array designed from expressed sequence tag (EST) sequences of the cormorant liver, where 1061 unique oligonucleotides were spotted. Common cormorants were collected from Lake Biwa, Japan in May 2001 and 2002. With the use of this oligo array, gene expression profiles in the liver of individual specimens were evaluated. To determine the expression patterns of genes altered by environmental contaminants, relationships between concentrations of persistent organochlorines including polychlorinated dibenzo-p-dioxins, furans, polychlorinated biphenyls, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane and its metabolites (DOTS), hexachlorocyclohexane isomers (HCHs), chlordane compounds (CHLs), butyltins, and bisphenol A (BPA) and expression levels of each gene in the cormorant liver were examined using stepwise multiple regression analysis. The reliability of data obtained by the oligo array was further confirmed by quantifying the expression levels of certain genes using real-time RTPCR. The 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent (TEQ) level was positively correlated with both cytochrome P4501A4 and 1A5 gene expression. In addition, the mRNA level of an antioxidant enzyme, Cu/Zn superoxide dismutase, was negatively correlated with hepatic total TEQ. Other antioxidant enzymes, glutathione peroxidase 3 and glutathione S-transferase class mu, were negatively correlated with HCHs and BPA levels, respectively. The mRNA expression level of a nonenzymatic antioxidant, haptoglobin, was negatively but not significantly correlated with CHLs. These results led to a hypothesis that wild cormorant population may suffer from oxidative stress due to chemically induced formation of reactive oxygen species and subsequent reduction of antioxidant resistance. Thus, the cormorant oligo array may be a useful monitoring tool to identify specific gene expression profiles altered by various environmental contaminants. Although further research is required to clarify a definitive cause-and-effect relationship, the current study provides valuable information on contaminant-responsive genes to predict potential effects on wildlife in a real environment.
  • Effect of tributyltin on reproduction in Japanese whiting, Sillago japonica, Yohei Shimasaki, Yuji Oshima, Suguru Inoue, Yoshiyuki Inoue, Ik Joon Kang, Kei Nakayama, Hisaya Imoto, Tsuneo Honjo, MARINE ENVIRONMENTAL RESEARCH, ELSEVIER SCI LTD, MARINE ENVIRONMENTAL RESEARCH, 62, S245, S248, 2006年, 0141-1136, 10.1016/j.marenvres.2006.04.030, We examined the effect of tributyltin (TBT) on reproduction in the Japanese whiting, Sillago japonica. Mature fish were placed in indoor 500-L polyethylene tanks (five males and three females per tank) with a flow-through system and received dietary exposure to tributyltin oxide at concentrations of 2, 20, or 200 mu g/g for 30 days during the active spawning period. Eggs spawned from the fish were collected daily, and the floating egg rate, larval deformity, hatchability, and viable hatch were monitored. TBT concentration in eggs of 200-mu g/g group ranged from 85.0 to 159.6 ng/g in the evaluation period (days 5-30). In this period, the floating egg rate (83.2%), viable hatchability (82.2%), and total number of viable larvae (422,000 larvae per 100 g of female) were all significantly decreased in the 200-mu g/g group compared with the control group (93.0%, 91.9%, and 709,000 larvae, respectively). The rate of deformity (2.6%) in the 200-mu g/g group was about three times that in the control group (0.8%), although this difference was not significant. From these results, the lowest observed effect concentration of TBT in eggs on reproduction in Japanese whiting was estimated to be less than 159.6 ng/g-eggs. (c) 2006 Elsevier Ltd. All rights reserved.
  • A Toxicogenomics Approach for Assessing Toxic Effects of POPs, 9, 2, 81, 86, 2006年
  • Effect of tributyltin on reproduction in Japanese whiting, Sillago japonica, Yohei Shimasaki, Yuji Oshima, Suguru Inoue, Yoshiyuki Inoue, Ik Joon Kang, Kei Nakayama, Hisaya Imoto, Tsuneo Honjo, MARINE ENVIRONMENTAL RESEARCH, ELSEVIER SCI LTD, MARINE ENVIRONMENTAL RESEARCH, 62, S245, S248, 2006年, 0141-1136, 10.1016/j.marenvres.2006.04.030, We examined the effect of tributyltin (TBT) on reproduction in the Japanese whiting, Sillago japonica. Mature fish were placed in indoor 500-L polyethylene tanks (five males and three females per tank) with a flow-through system and received dietary exposure to tributyltin oxide at concentrations of 2, 20, or 200 mu g/g for 30 days during the active spawning period. Eggs spawned from the fish were collected daily, and the floating egg rate, larval deformity, hatchability, and viable hatch were monitored. TBT concentration in eggs of 200-mu g/g group ranged from 85.0 to 159.6 ng/g in the evaluation period (days 5-30). In this period, the floating egg rate (83.2%), viable hatchability (82.2%), and total number of viable larvae (422,000 larvae per 100 g of female) were all significantly decreased in the 200-mu g/g group compared with the control group (93.0%, 91.9%, and 709,000 larvae, respectively). The rate of deformity (2.6%) in the 200-mu g/g group was about three times that in the control group (0.8%), although this difference was not significant. From these results, the lowest observed effect concentration of TBT in eggs on reproduction in Japanese whiting was estimated to be less than 159.6 ng/g-eggs. (c) 2006 Elsevier Ltd. All rights reserved.
  • Effects of polychlorinated biphenyls on the schooling behavior of Japanese medaka (Oryzias latipes), K Nakayama, Y Oshima, K Hiramatsu, Y Shimasaki, T Honjo, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, SETAC, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24, 10, 2588, 2593, 2005年10月, 0730-7268, 10.1897/04-518R2.1, The effects of polychlorinated biphenyls (PCBs; Kanechlor 400) on the schooling behavior of Japanese medaka (Oryzias latipes) were tested after feeding with various concentrations of PCBs (0, 1, 5, 25, and 125 mu g/g). To test schooling, three PCB-exposed medaka and three untreated fish were placed in one chamber, and their swimming trajectories were recorded. Swimming velocity, turning angle, and nearest-neighbor distance (NND) were analyzed individually, and the polarization and expanse of each schooling group were analyzed. Fractal dimension analysis was performed for trajectory, swimming velocity, and turning angle. Six behaviors (school formed by six swimming medaka, school formed by six stationary medaka, school formed by three to five swimming medaka, school formed by three to five stationary medaka, swimming without schooling, and stationary without schooling) were quantified with time. Behavioral parameters were the same for PCB-exposed and unexposed medaka in the same schooling group. Swimming velocity decreased in a dose-dependent manner, and NND in the group exposed to 25 mu g/g of PCBs was significantly longer (126%) than that in the controls. The fractal dimensions of trajectory and turning angle increased significantly in the highest PCB-exposure group, indicating that schools containing PCB-exposed individuals frequently changed direction. The PCBs shortened the time of school formed by six swimming fish, which was 23.7% of the control value in the group exposed to 25 mu g/g of PCBs. The frequency of collisions between individuals was significantly greater in the highest-exposure group (31.3) than in the controls (13.3). Thus, PCB exposure influences the behavior of unexposed fish in the same school, consequently affecting schooling behavior.
  • Early-life-stage toxicity in offspring from exposed parent medaka, <i>Oryzias latipes</i>, to mixtures of tributyltin and polychlorinated biphenyls, NAKAYAMA K, OSHIMA Y, NAGAFUCHI K, HANO T, SHIMASAKI Y, HONJO T, Environmental Toxicology and Chemistry, Environmental Toxicology and Chemistry, 24, 3, 591, 596, 2005年03月, 0730-7268, 10.1897/04-157R.1
  • Early-life-stage toxicity in offspring from exposed parent medaka, Oryzias latipes, to mixtures of tributyltin and polychlorinated biphenyls, K Nakayama, Y Oshima, K Nagafuchi, T Hano, Y Shimasaki, K Nakayamaj, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, SETAC, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 24, 3, 591, 596, 2005年03月, 0730-7268, 10.1897/04-157R.1, The present study examined the effects of tributyltin (TBT), polychlorinated biphenyls (PCBs), and mixtures of both chemicals on reproduction in Japanese medaka, Oryzias latipes. For 21 d we gave groups of medaka freeze-dried brine shrimp flakes contaminated with a mixture of either 0, 1, 5, or 25 mug TBT g(-1) plus 0 or 25 mug PCBs g(-1). We measured the fecundity and fertility of the parent fish and assessed the deformity, hatchability, time-to-hatching, and swim-up failure rate of the next generation. Fertilization success in the third week of the administration period was significantly decreased by administration of 25 mug TBT g(-1) (77%) compared with the control group (87%). Both TBT and PCBs were transferred maternally into the eggs of the next generation, causing early life-stage toxicity. Administration of 1 mug TBT g(-1) was not toxic to embryological development, but abnormal eye development (i.e., small eyes or no eyes) occurred when TBT at the same concentration was mixed with PCBs (6.4%). Administration of TBT alone significantly decreased hatchability and increased swim-up failure, and administration of PCBs alone significantly increased time-to-hatching. Statistical analysis by two-way analysis of variance detected an interaction between TBT and PCBs in these three parameters. TBT induces abnormal development of the eyes, reduced hatchability, and increased swim-up failure, whereas PCBs delay time-to-hatching. Administration of mixtures of TBT and PCBs has more adverse effects on the developmental stage of medaka than does that of each chemical alone.
  • Effects of polychlorinated biphenyls on the schooling behavior of Japanese medaka, <i>Oryzias latipes</i>, Environmental Toxicology and Chemistry, Environmental Toxicology and Chemistry, 24, 10, 2588, 2593, 2005年, 0730-7268, 10.1897/04-518R2.1
  • マイクロアレイを用いたカワウにおける潜在的毒性影響の評価 肝臓中の遺伝子発現プロファイルと化学物質蓄積レベルとの関係, 仲山 慶, 岩田 久人, 金 恩英, 久保田 彰, 田代 康介, 田辺 信介, 環境ホルモン学会研究発表会要旨集, 環境ホルモン学会(日本内分泌撹乱化学物質学会), 環境ホルモン学会研究発表会要旨集, 7回, 107, 107, 2004年12月
  • Fertilization success and sexual behavior in male medaka, Oryzias latipes, exposed to tributyltin, K Nakayama, Y Oshima, T Yamaguchi, Y Tsuruda, IJ Kang, M Kobayashi, N Imada, T Honjo, CHEMOSPHERE, PERGAMON-ELSEVIER SCIENCE LTD, CHEMOSPHERE, 55, 10, 1331, 1337, 2004年06月, 0045-6535, 10.1016/j.chemoshere.2003.11.050, We evaluated the effects of tributyltin (TBT), polychlorinated biphenyls (PCBs), and a mixture of TBT and PCBs on reproduction, gonadal histology, and sexual behavior in Japanese medaka (Oryzias latipes). We administered TBT (I mug g(-1) body weight [b.w.] daily), PCBs (1 mug g(-1) b.w. daily), or both to medaka for 3 weeks. We assessed reproductive success during week 3 and the sexual behavior of male medaka after the exposure period. Fertilization success was significantly decreased in groups that received TBT. The profiles of fertility developed a bimodal distribution in the TBT and TBT + PCBs groups, and the numbers of males that performed &quot;following&quot; and &quot;dancing&quot; were significantly decreased in the TBT + PCBs group. The frequency of &quot;dancing&quot; also decreased after treatment with TBT, whereas fish that received PCBs only showed no difference in sexual behavior. Testes that had oocytes showed nor-mat spermatogenesis, even though two of the five males treated with TBT + PCBs bad some indication of testis-ova, which did not occur in any other group. Our results indicate that TBT, but not PCBs, affects sexual behavior and reproduction in medaka. (C) 2003 Elsevier Ltd. All rights reserved.
  • Fertilization success and sexual behavior in male medaka, Oryzias latipes, exposed to tributyltin, K Nakayama, Y Oshima, T Yamaguchi, Y Tsuruda, IJ Kang, M Kobayashi, N Imada, T Honjo, CHEMOSPHERE, PERGAMON-ELSEVIER SCIENCE LTD, CHEMOSPHERE, 55, 10, 1331, 1337, 2004年06月, 0045-6535, 10.1016/j.chemoshere.2003.11.050, We evaluated the effects of tributyltin (TBT), polychlorinated biphenyls (PCBs), and a mixture of TBT and PCBs on reproduction, gonadal histology, and sexual behavior in Japanese medaka (Oryzias latipes). We administered TBT (I mug g(-1) body weight [b.w.] daily), PCBs (1 mug g(-1) b.w. daily), or both to medaka for 3 weeks. We assessed reproductive success during week 3 and the sexual behavior of male medaka after the exposure period. Fertilization success was significantly decreased in groups that received TBT. The profiles of fertility developed a bimodal distribution in the TBT and TBT + PCBs groups, and the numbers of males that performed &quot;following&quot; and &quot;dancing&quot; were significantly decreased in the TBT + PCBs group. The frequency of &quot;dancing&quot; also decreased after treatment with TBT, whereas fish that received PCBs only showed no difference in sexual behavior. Testes that had oocytes showed nor-mat spermatogenesis, even though two of the five males treated with TBT + PCBs bad some indication of testis-ova, which did not occur in any other group. Our results indicate that TBT, but not PCBs, affects sexual behavior and reproduction in medaka. (C) 2003 Elsevier Ltd. All rights reserved.
  • Alteration of general behavior of male medaka, Oryzias latipes, exposed to tributyltin and/or polychlorinated biphenyls, K Nakayama, Y Oshima, K Hiramatsu, T Honjo, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 49, 1, 85, 92, 2004年02月, 0023-6152, We examined the general behavior of male Japanese medaka, Oryzias latipes, after exposed to tributyltin (TBT), polychlorinated biphenyls (PCBs), or a mixture of these chemicals at a concentration of 1 mug/g body weight daily for 3 weeks. We analyzed swimming velocity as an indicator of acute toxicity and counted the frequencies of straight swimming and swimming in circles. We also calculated the entropy of the positions of the fish within the experimental chamber. Neither TBT nor PCBs nor their mixture affected the swimming velocity, suggesting that there were no acute toxic effects. However, PCBs exposure increased the frequencies of both straight and circular swimming, suggesting hyperactivity. TBT increased the positional entropy value. Both TBT and PCBs affect the general behavior of mature male medaka, although exposure to the 2 chemicals together has no significant additive behavioral effect.
  • Alteration of general behavior of male medaka, Oryzias latipes, exposed to tributyltin and/or polychlorinated biphenyls, K Nakayama, Y Oshima, K Hiramatsu, T Honjo, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 49, 1, 85, 92, 2004年02月, 0023-6152, We examined the general behavior of male Japanese medaka, Oryzias latipes, after exposed to tributyltin (TBT), polychlorinated biphenyls (PCBs), or a mixture of these chemicals at a concentration of 1 mug/g body weight daily for 3 weeks. We analyzed swimming velocity as an indicator of acute toxicity and counted the frequencies of straight swimming and swimming in circles. We also calculated the entropy of the positions of the fish within the experimental chamber. Neither TBT nor PCBs nor their mixture affected the swimming velocity, suggesting that there were no acute toxic effects. However, PCBs exposure increased the frequencies of both straight and circular swimming, suggesting hyperactivity. TBT increased the positional entropy value. Both TBT and PCBs affect the general behavior of mature male medaka, although exposure to the 2 chemicals together has no significant additive behavioral effect.
  • Effect of tributyltin on reproduction of bivalves and fish, Proceedings in International Symposium on Antifouling Paints and Marine Environment, Proceedings in International Symposium on Antifouling Paints and Marine Environment, 124, 132, 2004年
  • Effects of PCDDs/DFs/Co-PCB on hepatic protein expression profile in common cormorant population, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, 223, 227, 2004年
  • Monitoring of gene expression profiles related to chemical contamination in common cormorant (<i>Phalacrocorax carbo</i>) liver with an oligo array, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, 218, 222, 2004年
  • Distribution of tributyltin, dibutyltin and monobutyltin in the liver, brain and fat of rats: two-generation toxicity study of tributyltin chloride, Environmental Sciences, Environmental Sciences, 11, 123, 132, 2004年
  • Use of hepatic CYP1A1 activity in Japanese flounder to monitor oil dispersed from Nakhodka spill, Japanese Journal of Environmental Toxicology, Japanese Journal of Environmental Toxicology, 7, 2, 123, 129, 2004年, 1344-0667, 10.11403/jset.7.123
  • Relationship between temperature and pharmacokinetic parameters of florfenicol in carp (<i>Cyprinus carpio</i>), Japanese Journal of Environmental Toxicology, Japanese Journal of Environmental Toxicology, 7, 2, 61, 68, 2004年, 1344-0667, 10.11403/jset.7.61
  • Effect of tributyltin on reproduction of bivalves and fish, Proceedings in International Symposium on Antifouling Paints and Marine Environment, Proceedings in International Symposium on Antifouling Paints and Marine Environment, 124, 132, 2004年
  • Effects of PCDDs/DFs/Co-PCB on hepatic protein expression profile in common cormorant population, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, 223, 227, 2004年
  • Monitoring of gene expression profiles related to chemical contamination in common cormorant (<i>Phalacrocorax carbo</i>) liver with an oligo array, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, Proceedings of COE 1st International Symposium on Environmental Behavior and Ecological Impacts of Persistent Toxic Substances, 218, 222, 2004年
  • Distribution of tributyltin, dibutyltin and monobutyltin in the liver, brain and fat of rats: two-generation toxicity study of tributyltin chloride, Environmental Sciences, Environmental Sciences, 11, 123, 132, 2004年
  • Use of hepatic CYP1A1 activity in Japanese flounder to monitor oil dispersed from Nakhodka spill, Yuji Oshima, Jiro Koyama, Kei Nakayama, Yoshiyuki Inoue, Yohei Shimasaki, Suguru Inoue, Tsuneo Honjo, Japanese Journal of Environmental Toxicology, Japanese Journal of Environmental Toxicology, 7, 2, 123, 129, 2004年, 1344-0667, 10.11403/jset.7.123
  • Relationship between temperature and pharmacokinetic parameters of florfenicol in carp (<i>Cyprinus carpio</i>), Yuji Oshima, Tatsusuke Takeda, Sachie Katayama, Yoshiyuki Inoue, Suguru Inoue, Kei Nakayama, Yohei Shimasaki, Tsuneo Honjo, Japanese Journal of Environmental Toxicology, Japanese Journal of Environmental Toxicology, 7, 2, 61, 68, 2004年, 1344-0667, 10.11403/jset.7.61
  • トリブチルスズとPCBsと水産生物, 大嶋雄治, 島崎洋平, 井上英, 仲山慶, 本城凡夫, Endocr Disrupter News Lett, Endocr Disrupter News Lett, 6, 2, 6, 2003年06月, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902204918881553
  • DNAマイクロアレイを用いたポリ塩化ビフェニルによるメダカ脳内遺伝子発現変動の検出, 仲山慶, 大嶋雄治, 田代康介, 三田和英, 北野健, 岸田光代, 石川裕二, 永淵賢, 本城凡夫, 日本水産学会大会講演要旨集, 日本水産学会大会講演要旨集, 2003, 162, 2003年04月, http://jglobal.jst.go.jp/public/200902295419973507
  • 最新 環境ホルモン研究 沿岸域におけるトリブチルスズ汚染の現状と魚介類への影響, 大嶋雄治, 井上英, 島崎洋平, 仲山慶, 本城凡夫, 海洋と生物, 海洋と生物, 25, 1, 11, 14, 2003年02月, 0285-4376, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902217924183706
  • 沿岸域におけるトリブチルスズ汚染の現状と魚介類への影響, 海洋と生物, 海洋と生物, 25, 1, 11, 14, 2003年
  • Monitoring of dispersed oil pollution by P4501A1 activity in fish, Proceedings of the Second International Work Shop on Bioassay of the Marine Environment in Northwest Pacific Region, Proceedings of the Second International Work Shop on Bioassay of the Marine Environment in Northwest Pacific Region, 74, 77, 2003年
  • Suppression of sexual behavior in male Japanese medaka (<i>Oryzias latipes</i>) exposed to 17β-estradiol, OSHIMA Y, KANG I J, KOBAYASHI M, NAKAYAMA K, IMADA N, HONJO T, Chemosphere, Chemosphere, 50, 3, 429, 436, 2003年01月, 0045-6535, 10.1016/S0045-6535(02)00494-0
  • Monitoring of dispersed oil pollution by P4501A1 activity in fish, Proceedings of the Second International Work Shop on Bioassay of the Marine Environment in Northwest Pacific Region, Proceedings of the Second International Work Shop on Bioassay of the Marine Environment in Northwest Pacific Region, 74, 77, 2003年
  • Suppression of sexual behavior in male Japanese medaka (Oryzias latipes) exposed to 17 beta-estradiol, Y Oshima, IJ Kang, M Kobayashi, K Nakayama, N Imada, T Honjo, CHEMOSPHERE, PERGAMON-ELSEVIER SCIENCE LTD, CHEMOSPHERE, 50, 3, 429, 436, 2003年01月, 0045-6535, 10.1016/S0045-6535(02)00494-0, Mating pairs of Japanese medaka (Oryzias latipes) received 17beta-estradiol (E2; dose, 3 and 30 mug/g body weight daily) in their diet and E2-free diet (control) for two weeks. The effect on reproductive success was assessed in the mating pairs, and sexual and nonsexual behaviors in the male were quantified. The fecundities of the E2-treated groups were reduced significantly (p &lt; 0.05) compared with those of control, and the sexual behaviors (following, dancing, floating, and crossing) of male fish in response to prostaglandin F2alpha (PG)-injected E2-unexposed females were suppressed dramatically. While the fertility of spawned eggs and the frequencies of the nonsexual behaviors of resting and swimming in a straight line performed by male were unchanged in E2-exposed groups. Therefore, sexual behavior in male may be linked with reproductive success in mating pair of madaka exposed to E2. This assay for sexual behavior using PG may provide a useful tool for assessing the effect of endocrine-disrupting chemicals. (C) 2002 Elsevier Science Ltd. All rights reserved.
  • メダカの行動解析によるバイオモニタリングの試み, 平松和昭, 仲山慶, 大嶋雄治, 四ケ所四男美, 本城凡夫, 応用水文, 応用水文, 15, 88, 95, 2002年10月, 1881-4948, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902166139890300
  • メダカの行動解析によるバイオモニタリングの試み, 応用水文, 応用水文, 15, 88, 95, 2002年
  • トリブチルスズ(TBT)の魚類血液への蓄積とTBT‐PCB複合汚染による産卵・ふ化への影響, 大嶋雄治, NIMALA K, 横田佳子, 島崎洋平, 井上英, 仲山慶, 今田信良, 本城凡夫, 小林邦男, 日本海水学会誌, 日本海水学会誌, 53, 4, 224, 228, 1999年08月, 0369-4550, 10.11457/swsj1965.53.224, http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902164485090171
  • トリブチルスズ(TBT)の魚類血液への蓄積とTBT-PCB複合汚染による産卵・ふ化への影響, 日本海水学会誌, 日本海水学会誌, 53, 4, 224, 228, 1999年, 10.11457/swsj1965.53.224

その他研究情報

競争的資金

  • 日本学術振興会, 科学研究費助成事業 基盤研究(C), 感染症の発症をエンドポイントとした魚類免疫毒性評価系の確立, 仲山 慶
  • 日本学術振興会, 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B)), マレーシア沿岸・河口域底質汚染の毒性リスクと生物多様性へのインパクトの包括的評価, 宇野 誠一, 仲山 慶, 國師 恵美子
  • 日本学術振興会, 科学研究費助成事業 挑戦的研究(萌芽), 分泌性病原因子を用いた新規魚類寄生虫ワクチンの開発, 北村 真一, 仲山 慶
  • 日本学術振興会, 科学研究費助成事業 基盤研究(C), 下水処理水に残留する医薬品等による魚類の感染症誘発に対するリスク評価, 仲山 慶, 本研究は,魚類を対象として感染症の発症を主要なエンドポイントとした免疫毒性評価手法を確立し,下水処理水中に含まれる医薬品等の感染症の発症リスクへの寄与を明らかにすることを目的としている。 平成30年度も前年度に引き続き,愛媛県内の下水処理場の放流水を定期的に採取し,医薬品および生活関連化学物質(PPCPs)のモニタリングを実施した。モニタリング調査の結果,解熱鎮痛消炎剤や一部の抗菌剤などが高頻度かつ比較的高濃度で検出され,前年度の調査結果と一致していた。 前年度までに確立した感染実験にて,死亡した個体からAeromonas salmonicidaを定量的PCR法にて検出するための条件検討を行った。魚体重約0.6 gのコイに対して本菌を感染させ,死亡個体から腹水を採取した。また,その他の発症個体(うち一部は死亡個体)の全魚体,鰓または内臓を採取し,DNAを抽出した。腹水および抽出DNAをテンプレートとして複数条件にてPCRを行ったが,A. salmonicidaは検出されなかった。このことから,本試験で用いたA. salmonicidaは全身感染を起こさず,局所的に病変部にて増殖するものと考えられた。 また,先行研究にてリポ多糖の存在下で魚体中への化学物質の取込量が増加する可能性が示されていたため,本研究においてもA. salmonicidaに感染したコイで医薬品の取込量が増えるかを,3種の医薬品の混合物を対象に検証した。その結果,3種の化合物のうち相対的に水溶性が高いものは,感染魚において60%ほど取込量が増加していた。一方,脂溶性が高い化合物は3割程度取込量が減少した。このことから,病原体への日和見感染が,易水溶性化学物質の取込量の増加による作用効果の増強を引き起こす可能性が示唆された。
  • 日本学術振興会, 科学研究費助成事業 基盤研究(B), マボヤ被嚢軟化症の防疫対策に関する研究, 北村 真一, 広瀬 裕一, 柳田 哲矢, 仲山 慶, マボヤ被嚢軟化症の防疫対策として、新規簡易診断法を確立することを目的とした。顕微鏡を用いたスタンプ法は、容易に未発症個体から原因鞭毛虫を検出することができ、現場での診断に有効であった。また、本虫の拡散をトレースできるマーカー遺伝子を探索し、集団遺伝学的解析を行った。GPI遺伝子およびマイクロサテライトマーカーを用いた解析から、日本特有のハプロタイプからなるグループと、韓国特有のハプロタイプと日韓両国から確認されるハプロタイプを含むグループに2分された。このことから、日韓には遺伝的にある程度分化したホヤムシ集団が分布する一方、近年韓国から日本に移入したと考えられる集団の存在も示唆された。
  • 文部科学省, 科学研究費補助金(若手研究(B)), 化学物質の慢性暴露が病原体感染時のコイの免疫応答に与える影響, 仲山 慶
  • 文部科学省, 科学研究費補助金(基盤研究(B)), マボヤ被嚢軟化症の被嚢軟化メカニズムの解明, 北村 真一, 広瀬 裕一, 山田 力志, 仲山 慶, マボヤ被嚢軟化症は同養殖に大きな被害を与えている.申請者らの研究で,本症はAzumiobodo hoyamushiと呼ばれる鞭毛虫によって起こることが明らかとなった.これまでに,被嚢の軟化メカニズムに関する知見はないが,予備実験から本虫のプロテアーゼがマボヤの被嚢セルロースを架橋する未知タンパク質を分解することで,軟化症が起こると考えられた.そこで本課題では,軟化メカニズムを解明するために,本虫のプロテアーゼを検出し,それらの組換え酵素と被嚢切片を用いて,in vitroで軟化症を再現する.さらに,病マボヤの被嚢のプロテオーム解析を行い,どのような過程で軟化が起こるのかをタンパク質レベルで明らかにする.最終的には,被嚢セルロースを架橋する未知タンパク質を同定し,病原体と宿主の双方の観点から,被嚢の軟化メカニズムを解明する.昨年度の成果として,A. hoyamushiが細胞外プロテアーゼを産生することを明らかにした.すなわち, 7種の人工基質(アミノペプチダーゼ型1種,トリプシン型2種,キモトリプシン型2種およびエラスターゼ型2種)を用いて,本虫の細胞外プロテアーゼの検出を行ったところ,アミノペプチダーゼ型,トリプシン型およびキモトリプシン型のプロテアーゼが,それぞれ1種,2種および1種検出された.これらの中でも,トリプシン型プロテアーゼの活性が高いことが明らかにされた.一方,エラスターゼ型のプロテアーゼは検出されなかった.次に,被嚢軟化が起こるメカニズムを解明するために感染個体の被嚢のプロテオーム解析を行った.その結果,発症個体特異的に減少するタンパク質として,筋原線維安定化タンパク質や2種のアクチンなどが検出された.今後は,これらのタンパク質を人工的に合成し,前述のプロテアーゼによる分解実験および被嚢中での局在を明らかにしていく予定である.
  • 文部科学省, 科学研究費補助金(若手研究(B)), リンホシスチスウイルス感染による宿主細胞の肥大化機構の解明, 北村 真一, 仲山 慶, リンホシスチス病は,ヒラメ養殖に甚大な被害をもたらしている.本病は最も古くから知られている魚類ウイルス病にも関わらず,その発症機構については不明な点が多い.本研究では,ヒラメにリンホシスチスウイルス(LCDV)を実験感染し,宿主魚類の遺伝子発現変化を経時的に調べ本症の発生機構を考察した.ウイルス感染後0,1,3,7,14,21,28,42および56日目(dpi)にそれぞれの区から5個体ずつ実験魚をサンプリングした.ヒラメの遺伝子発現量変化は,マイクロアレイ実験で調べた.その結果,28dpiまでは,ほとんどの遺伝子で発現量変化は認められなかった.しかしながら,42dpiでは,900個以上の遺伝子において発現量変化が観察された.これらのほとんどは発現が抑制されており,特にアポトーシス誘導および細胞周期調節に関連する遺伝子群の発現が抑制されていた.本研究で得られた遺伝子発現変化は,DNA腫瘍ウイルスに感染した宿主のものと酷似していたことから,リンホシスチス細胞の形成は腫瘍形成と類似した機構によるものと考えられた.
  • 文部科学省, 科学研究費補助金(基盤研究(S)), アジア途上地域における POPs 候補物質の汚染実態解明と生態影響評価, 田辺 信介, 岩田 久人, 高菅 卓三, 高橋 真, 仲山 慶, 滝上 英孝, 磯部 友彦, 鈴木 剛, POPs候補物質(有機臭素系難燃剤など)に注目し、途上地域を中心にその分析法の開発、広域汚染の実態解明、廃棄物投棄場等汚染源の解析、生物蓄積の特徴、バイオアッセイ等による影響評価、過去の汚染の復元と将来予測のサブテーマに取り組み、環境改善やリスク軽減のための科学的根拠を国際社会に提示するとともに、当該研究分野においてアジアの広域にまたがる包括的な情報を蓄積することに成功した。
  • 文部科学省, 科学研究費補助金(若手研究(B)), ヒラメにおける水酸化PCBsの体内挙動と生体影響に関する研究, 仲山 慶, 本研究では,ポリ塩化ビフェニル(PCBs)の代謝物である水酸化PCBs(OH-PCBs)が,ヒラメの初期胚に対して及ぼす影響を解析した。その結果,OH-PCBsの一部の異性体(4-hydroxy-2',3,5,5'-tetrachlorobiphenyl)は0.1ppb以上の濃度で発生異常,特に頭部末梢神経の形態的異常を引き起こすことが明らかとなった。本研究結果より,OH-PCBsがin vivoにおいても甲状腺ホルモンの機能をかく乱する可能性が示唆された。
  • 文部科学省, 科学研究費補助金(萌芽研究), 野生カワウ個体群のエコトキシコゲノミクス, 岩田 久人, 金 恩英, 仲山 慶, フッ素置換化合物(perfluorinated compounds : PFCs)の毒性影響に関する情報は限定されており,ヒトや野生生物へのリスクを評価するには不十分である。本研究では,オリゴアレイより得られた遺伝子発環プロファイルデータを用いて,PFCs濃度依存的に発現が変動する遺伝子群をスクリーニングし,PFCsによる毒性影響を予測した。さらに,同一個体で測定したダイオキシン類の蓄積濃度データを解析に加え,PFCsとダイオキシン類による複合毒性影響の評価を試みた。遺伝子発現レベルとPFCs蓄積濃度との関係を解析した結果,PFCs応答遺伝子の候補として74種類の遺伝子が挙げられた。そのうち,glutathione peroxidase 1(GPX1), glutathione S-transferase alpha 3(GSTA3), heat shock 70 kDa protein 8(HSPA8), tumor rejection antigen 1(TRA1)のmRNAレベルをreal-time RT-PCRで定量したところ,PFOS蓄積濃度とGPX1およびGSTA3の間に正の関係が,PFOSとHSPA8およびTRA1の間に負の関係が検出された。したがって,PFOSあるいはその他のPFCsによって,これらの遺伝子はそれぞれ正または負の制御を受けていると推察された。また、重回帰分析の結果,7種類の遺伝子の発現量は,PFCsおよびダイオキシン類の蓄積濃度によって説明可能であり,それらは複合的な影響を受けていることが示唆された。例えば,CYP1A mRNA発現量は,PFNAと2,3,7,8-TCDD毒性換算等量によって説明された。さらにCYP1Aタンパク質発現量およびEROD活性も同様であった。

愛媛大学教員活動実績

教育活動(B)

担当授業科目(B01)

  • 2019年, 前期, 学部, 基礎生物学演習
  • 2019年, 前期, 学部, 生物学ゼミナールⅠ
  • 2019年, 前期, 学部, 生物学特別演習Ⅰ
  • 2019年, 前期, 学部, 海洋生物学実習
  • 2019年, 前期, 学部, 卒業研究Ⅰ
  • 2019年, 前期, 学部, 基礎生物学実験
  • 2019年, 前期, 学部, 基礎生物学実験
  • 2019年, 前期, 学部, 基礎生物学実験


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