研究者総覧

水川 葉月 (ミズカワ ハズキ)

  • 大学院農学研究科 生物環境学専攻 准教授
Last Updated :2021/01/20

研究者情報

学位

  • 博士(理学)(愛媛大学)

論文上での記載著者名

  • Hazuki Mizukawa

ホームページURL

J-Global ID

研究キーワード

  • 歩哨動物   農薬   内分泌かく乱   環境毒性学   野生動物   分析化学   環境汚染   PBDEs   PCBs   ペット動物   リスク評価   POPs   室内汚染   陸棲哺乳類   

研究分野

  • 環境・農学 / 環境影響評価

経歴

  • 2018年07月 - 現在  愛媛大学大学院農学研究科環境保全学コース准教授
  • 2018年04月 - 2018年06月  北海道大学大学院獣医学研究院博士研究員
  • 2013年04月 - 2018年03月  北海道大学大学院獣医学研究院助教
  • 2011年04月 - 2013年03月  独立行政法人日本学術振興会 特別研究員DC2
  • 2010年04月 - 2013年03月  愛媛大学大学院理工学研究科博士後期課程

研究活動情報

論文

  • Kraisiri Khidkhan, Yoshinori Ikenaka, Takahiro Ichise, Shouta M M Nakayama, Hazuki Mizukawa, Kei Nomiyama, Hisato Iwata, Koji Arizono, Keisuke Takahashi, Keisuke Kato, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 239 108898 - 108898 2021年01月 研究論文(学術雑誌) 
    Neonicotinoid insecticides are used for agricultural and non-agricultural purposes worldwide. Pets are directly exposed to neonicotinoids in veterinary products and through environmental contamination. Cytochrome P450 (CYP) is among the most significant xenobiotic metabolizing enzymes that oxidizes several chemicals, including neonicotinoids. However, CYP activities and metabolite compositions of neonicotinoid metabolites are unknown in most domesticated pet species. Our objectives were to reveal the differences in metabolites of neonicotinoids (imidacloprid, clothianidin, and acetamiprid) and CYP activities among common pet species (cats and dogs), humans, and rats. The results indicated that the CYP-mediated neonicotinoid metabolism was different depending on species and each neonicotinoid. Among these four species, the kinetics of imidacloprid metabolism indicated that rats have the highest rate of oxidation of imidacloprid to 4OH-imidacloprid, while the greatest enzyme kinetics of imidacloprid metabolism to 5OH-imidacloprid were found in rats and humans. Clothianidin was rapidly metabolized to 1-methyl-3-nitroguanidine and dm-clothianidin in rats, but cats and humans showed the lowest formation of dm-clothianidin. CYP activities in metabolism of acetamiprid to dm-acetamiprid and N-acetyl-acetamiprid were determined to be significantly higher in humans compared to other species. However, further studies should be targeted at identifying the differences in hepatic metabolism of neonicotinoids in these species using recombinant CYP enzymes.
  • Kraisiri Khidkhan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M M Nakayama, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    The Journal of veterinary medical science 82 7 978 - 982 2020年07月 [査読有り]
     研究論文(学術雑誌) 
    The knowledge of cytochrome P450 (CYP) expression involved in chemical exposure are necessary in clinical applications for the medication and prediction of adverse effects. The aim of this study was to evaluate the mRNA expression of CYP1-CYP3 families in cats exposed to BDE-209 for one year. All selected CYP isoforms showed no significant difference in mRNA expressions between control and exposure groups, however, CYP3A12 and CYP3A131 revealed tend to be two times higher in the exposure group compared to control group. The present results indicate that the chronic exposure of BDE209 could not alter CYP expression in the liver of cats. This result considered caused by the deficiency of CYP2B subfamily which is major metabolism enzyme of polybrominated diphenyl ethers (PBDEs) in cat.
  • Kosuke Tanaka, Yutaka Watanuki, Hideshige Takada, Mayumi Ishizuka, Rei Yamashita, Mami Kazama, Nagako Hiki, Fumika Kashiwada, Kaoruko Mizukawa, Hazuki Mizukawa, David Hyrenbach, Michelle Hester, Yoshinori Ikenaka, Shouta M M Nakayama
    Current biology : CB 30 4 723 - 728 2020年02月 [査読有り]
     研究論文(学術雑誌) 
    Plastic debris is ubiquitous and increasing in the marine environment [1]. A wide range of marine organisms ingest plastic, and its impacts are of growing concern [2]. Seabirds are particularly susceptible to plastic pollution because of high rates of ingestion [3]. Because marine plastics contain an array of hazardous compounds, the chemical impacts of ingestion are concerning. Several studies on wild seabirds suggested accumulation of plastic-derived chemicals in seabird tissues [4-7]. However, to date, the evidence has all been indirect [4-7], and it is unclear whether plastic debris is the source of these pollutants. To obtain direct evidence for the transfer and accumulation of plastic additives in the tissues of seabirds, we conducted an in vivo plastic feeding experiment. Environmentally relevant exposure of plastics compounded with one flame retardant and four ultraviolet stabilizers to streaked shearwater (Calonectris leucomelas) chicks in semi-field conditions resulted in the accumulation of the additives in liver and adipose fat of 91 to 120,000 times the rate from the natural diet. Additional monitoring of six seabird species detected these chemical additives only in those species with high plastic ingestion rates, suggesting that plastic debris can be a major pathway of chemical pollutants into seabirds. These findings provide direct evidence of seabird exposure to plastic additives and emphasize the role of marine debris ingestion as a source of chemical pollution in marine organisms.
  • Shouta M M Nakayama, Ayuko Morita, Yoshinori Ikenaka, Yusuke K Kawai, Kensuke P Watanabe, Chihiro Ishii, Hazuki Mizukawa, Yared B Yohannes, Keisuke Saito, Yukiko Watanabe, Masaki Ito, Natsuo Ohsawa, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 228 108635 - 108635 2020年02月 [査読有り]
     研究論文(学術雑誌) 
    Worldwide use of anticoagulant rodenticides (ARs) for rodents control has frequently led to secondary poisoning of non-target animals, especially raptors. In order to suggest some factors that may help considering the mechanism of the incidents, this study focused on the avian vitamin K 2, 3-epoxide reductase (VKOR) that is the target protein of ARs. We addressed the interspecific differences in VKOR activity and inhibition related to amino acid sequence and mRNA expression of VKORC1 and VKORC1-like1 (VKORC1L1). Poultry have been considered to be more tolerant to ARs than mammals. However, VKOR activity of owls, hawks, falcon and surprisingly, canaries, was lower and inhibited by warfarin more easily than that of chickens and turkeys. The amino acid sequence of VKORC1 and VKORC1L1 implied that the value of Ki for VKOR activity to ARs could depend on the amino acid at position 140 in the TYX warfarin-binding motif in VKORC1, and other amino acid mutations in VKORC1L1. The mRNA expression ratio of VKORC1:VKORC1L1 differed between turkey (8:1) and chicken (2:3) liver. VKORC1L1 has been reported to be resistant to warfarin compared to VKORC1. Hence, both the Ki of specific VKORC1 and VKORC1L1, and the mRNA expression ratio would cause avian interspecific difference of the VKOR inhibition. Our study also suggested the high inhibition of VKOR activities in raptors and surprisingly that in canaries as well. These factors are the most likely to contribute to the high sensitivity to ARs found in raptors.
  • Masao Togao, Shouta M M Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yoshiki Makino, Ayano Kubota, Takehisa Matsukawa, Kazuhito Yokoyama, Takafumi Hirata, Mayumi Ishizuka
    Chemosphere 238 124581 - 124581 2020年01月 [査読有り]
     研究論文(学術雑誌) 
    Lead (Pb) pollution is one of the most serious environmental problems and has attracted worldwide attention. Pb causes hematological, central nervous system, as well as renal toxicity, and so on. Although many investigations about Pb in blood to evaluate pollution status and toxic effects have been reported, there are open question about biological behavior of Pb. In order to reveal any toxicological mechanisms or influences, we focused on the local distribution of Pb in mice organs. Lead acetate (100 mg/L and 1000 mg/L) in drinking water were given to the BALB/c mice (male, seven weeks of age, N = 24) for three weeks. Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) analysis revealed a homogenous distribution of Pb in the liver and inhomogeneous distribution in the kidney and brain. The hippocampus, thalamus, and hypothalamus had higher concentrations than other areas such as the white matter. Surprisingly, in the kidney, Pb tended to accumulate in the medulla rather than the cortex, strongly suggesting that high sensitivity areas and high accumulation areas differ. Moreover, distribution of stromal interacting protein 1 (STIM1) which is candidate gene of Pb pathway to the cells was homogenous in the liver and kidney whereas inhomogeneous in the brain. In contrast to our hypothesis, interestingly, Pb exposure under the current condition did not induce mRNA expressions for any candidate channel or transporter genes. Thus, further study should be conducted to elucidate the local distribution of Pb and other toxic metals, and pathway that Pb takes to the cells.
  • Kraisiri Khidkhan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M M Nakayama, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Wageh Sobhy Darwish, Mitsuyoshi Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 226 108613 - 108613 2019年12月 [査読有り]
     研究論文(学術雑誌) 
    Cats have been known to be extremely sensitive to chemical exposures. To understand these model species' sensitivity to chemicals and their toxicities, the expression profiles of xenobiotic-metabolizing enzymes should be studied. Unfortunately, the characterization of cytochrome P450 (CYP), the dominant enzyme in phase I metabolism, in cats has not extensively been studied. Polychlorinated biphenyls (PCBs) are known as CYP inducers in animals, but the information regarding the PCB-induced CYP expression in cats is limited. Therefore, in the present study, we aimed to elucidate the mRNA expression of the CYP1-CYP3 families in the cat tissues and to investigate the CYP mRNA expression related to PCB exposure. In cats, the greatest abundance of CYP1-CYP3 (CYP1A2, CYP2A13, CYP2C41, CYP2D6, CYP2E1, CYP2E2, CYP2F2, CYP2F5, CYP2J2, CYP2U1, and CYP3A132) was expressed in the liver, but some extrahepatic isozymes were found in the kidney (CYP1A1), heart (CYP1B1), lung (CYP2B11 and CYP2S1) and small intestine (CYP3A131). In cats, CYP1A1, CYP1A2 and CYP1B1 were significantly upregulated in the liver as well as in several tissues exposed to PCBs, indicating that these CYPs were distinctly induced by PCBs. The strong correlations between 3,3',4,4'-tetrachlorobiphenyl (CB77) and CYP1A1 and CYP1B1 mRNA expressions were noted, demonstrating that CB77 could be a potent CYP1 inducer. In addition, these CYP isoforms could play an essential role in the PCBs biotransformation, particularly 3-4 Cl-PCBs, because a high hydroxylated metabolite level of 3-4 Cl-OH-PCBs was observed in the liver.
  • Lesa A Thompson, Yoshinori Ikenaka, Wageh Sobhy Darwish, Shouta M M Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    Environmental toxicology and pharmacology 72 103249 - 103249 2019年11月 [査読有り]
     研究論文(学術雑誌) 
    The organochlorine pesticide dichloro-diphenyl-trichloroethane (DDT) is persistent in the environment and leads to adverse human health effects. High levels in breast milk pose a threat to both breast tissue and nursing infants. The objectives of this study were to investigate DDT-induced transcriptomic alterations in enzymes and transporters involved in xenobiotic metabolism, immune responses, oxidative stress markers, and cell growth in a human breast cancer cell line. MCF-7 cells were exposed to both environmentally-relevant and previously-tested concentrations of p,p'-DDT in a short-term experiment. Significant up-regulation of metabolizing enzymes and transporters (ACHE, GSTO1, NQO1 and ABCC2) and oxidative stress markers (CXCL8, HMOX-1, NFE2L2 and TNF) was clearly observed. Conversely, UGT1A6, AHR and cell growth genes (FGF2 and VEGFA) were severely down-regulated. Identification of these genes helps to identify mechanisms of p,p'-DDT action within cells and may be considered as useful biomarkers for exposure to DDT contamination.
  • Kohki Takaguchi, Hiroyuki Nishikawa, Hazuki Mizukawa, Rumi Tanoue, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shouta M M Nakayama, Yoshinori Ikenaka, Tatsuya Kunisue, Mayumi Ishizuka, Shinsuke Tanabe, Hisato Iwata, Kei Nomiyama
    The Science of the total environment 688 1172 - 1183 2019年10月 [査読有り]
     研究論文(学術雑誌) 
    Polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) might disrupt thyroid function. However, there is no clear evidence of PCB exposure disrupting thyroid hormone (TH) homeostasis in dogs and cats. The present study conducted in vivo experiments to evaluate the effects of a mixture of 12 PCB congeners (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187 and 202, each congener 0.5 mg/kg BW, i.p. administration) on serum TH levels in male dogs (Canis lupus familiaris) and male cats (Felis silvestris catus). In PCB-exposed dogs, the time courses of higher-chlorinated PCBs and L-thyroxine (T4)-like OH-PCBs (4-OH-CB107 and 4-OH-CB202) concentrations were unchanged or tended to increase, whereas those of lower-chlorinated PCBs and OH-PCBs tended to decrease after 24 h. In PCB-exposed cats, concentrations of PCBs increased until 6 h and then remained unchanged. The levels of lower-chlorinated OH-PCBs including 4'-OH-CB18 increased until 96 h and then decreased. In PCB-exposed dogs, free T4 concentrations were higher than those in the control group at 48 and 96 h after PCB administration and positively correlated with the levels of T4-like OH-PCBs, suggesting competitive binding of T4 and T4-like OH-PCBs to a TH transporter, transthyretin. Serum levels of total T4 and total 3,3',5-triiodo-L-thyronine (T3) in PCB-exposed dogs were lower than in the control group at 24 and 48 h and negatively correlated with PCB concentrations, implying that PCB exposure enhanced TH excretion by increasing TH uptake and TH conjugation enzyme activities in the dog liver. In contrast, no obvious changes in TH levels were observed in PCB-exposed cats. This could be explained by the lower levels of T4-like OH-PCBs and lower hepatic conjugation enzyme activities in cats compared with dogs. Different effects on serum TH levels in PCB-exposed dogs and cats are likely to be attributable to species-specific PCB and TH metabolism.
  • Kei Nomiyama, Akifumi Eguchi, Kohki Takaguchi, Jean Yoo, Hazuki Mizukawa, Tomoko Oshihoi, Shinsuke Tanabe, Hisato Iwata
    Toxicology and applied pharmacology 377 114620 - 114620 2019年08月 [査読有り]
     研究論文(学術雑誌) 
    Canis lupus familiaris (domestic dog) possess a high capacity to metabolize higher-chlorinated polychlorinated biphenyls (PCBs) to thyroid hormone (TH)-like hydroxylated PCB metabolites (OH-PCBs). As a result, the brain could be at high risk of toxicity caused by OH-PCBs. To evaluate the effect of OH-PCBs on dog brain, we analyzed OH-PCB levels in the brain and the metabolome of the frontal cortex following exposure to a mixture of PCBs (CB18, 28, 70, 77, 99, 101, 118, 138, 153, 180, 187, and 202). 4-OH-CB202 and 4-OH-CB107 were major OH-PCBs in the brain of PCB-exposed dogs. These OH-PCBs were associated with metabolites involved in urea cycle, proline-related compounds, and purine, pyrimidine, glutathione, and amino-acid metabolism in dog brain. Moreover, adenosine triphosphate levels in the PCBs exposure group were significantly lower than in the control group. These results suggest that OH-PCB exposure is associated with a disruption in TH homeostasis, generation of reactive oxygen species, and/or disruption of oxidative phosphorylation (OXPHOS) in brain cells. Among them, OXPHOS disturbance could be associated with both disruptions in cellular amino-acid metabolism and urea cycle. Therefore, an OXPHOS activity assay was performed to evaluate the disruption of OXPHOS by OH-PCBs. The results indicated that 4-OH-CB107 inhibits the function of Complexes III, IV, and V of the electron transport chain, suggesting that 4-OH-CB107 inhibit these complexes in OXPHOS. The neurotoxic effects of PCB exposure may be mediated through mitochondrial toxicity of OH-PCBs in the brain.
  • Momoko Ishiyama, Yuki Matsuo, Kunihiko Nakai, Nozomi Tatsuta, Haruhiko Nakata, Hazuki Mizukawa, Takashi Miyawaki, Hiromitsu Nagasaka, Takashi Someya, Daisuke Ueno
    Marine pollution bulletin 145 81 - 87 2019年08月 [査読有り]
     
    In the Great East Japan Earthquake of 11 March 2011, a magnitude 9.0 earthquake and accompanying tsunami struck the Tohoku region of Japan. The tsunami washed away old equipment containing polychlorinated biphenyls (PCBs) stored in the region's factories, and these PCBs may have leaked out of their casings into the marine environment. In this study, we evaluate marine PCB contamination by comparing mussels collected before (in 2005) and after (June 2011) the tsunami. PCB contamination levels were significantly elevated in mussel samples collected after the tsunami in June 2011 (3 months after the tsunami). This indicates that PCBs (specifically, formulation KC-400) leaked out of old equipment swept away by the tsunami and accumulated in mussels. PCBs were estimated to have an environmental half-life (EHL) in mussels of 4 months. Our results show that an earthquake and subsequent tsunami can cause elevated PCB contamination in the marine environment.
  • Y Ohara, A Yabuki, R Nakamura, O Ichii, H Mizukawa, N Yokoyama, O Yamato
    Journal of comparative pathology 170 53 - 59 2019年07月 [査読有り]
     研究論文(学術雑誌) 
    During the progression of chronic kidney disease (CKD), macrophage infiltration is a crucial event leading to tubulointerstitial fibrosis. In the present study, macrophages infiltrating renal tissue in dogs and cats with CKD were analysed immunohistochemically. Iba-1 was used as a pan-macrophage marker, CD204 was used as a marker of M2 macrophages and tumour necrosis factor (TNF)-α was used as a marker of M1 macrophages. Signals for Iba1 and CD204 were observed in the interstitium of all tested kidney samples. In dogs, the signals were diffusely scattered. In cats, both diffuse and focal signals were observed. Cells that were positive for Iba1 and CD204 were also observed in the tubular lumina in cats. Co-expression of Iba1 and CD204 was also observed in the infiltrating cells by immunofluorescence labelling, and these cells were negative for TNF-α. By quantitative analysis, the indices for Iba1- and CD204-positive cells were significantly correlated with the concentrations of plasma creatinine and/or urea and the extent of interstitial fibrosis in both dogs and cats. These results demonstrated that renal infiltration of M2 macrophages plays an important role in the progression of CKD in dogs and cats. The distribution pattern of the kidney-infiltrating macrophages was unique in cats and may be associated with a cat-specific renal fibrotic process.
  • Yusuke K Kawai, So Shinya, Yoshinori Ikenaka, Aksorn Saengtienchai, Takamitsu Kondo, Wageh Sobhy Darwish, Shota M M Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 217 5 - 14 2019年03月 [査読有り]
     
    Birds are exposed to many xenobiotics during their lifetime. For accurate prediction of xenobiotic-induced toxic effects on avian species, it is necessary to understand metabolic capacities in a comprehensive range of bird species. However, there is a lack of information about avian xenobiotic metabolizing enzymes (XMEs), particularly in wild birds. Uridine diphosphate glucuronosyltransferase (UGT) is an XME that plays an important role in phase II metabolism in the livers of mammals and birds. This study was performed to determine the characteristics of UGT1E isoform in avian species, those are related to mammals UGT 1A. To understand the characteristics of avian UGT1E isoforms, in vitro metabolic activity and genetic characteristics were investigated. Furthermore, mRNA expression levels of all chicken UGT1E isoforms were measured. On in vitro enzymatic analysis, the white-tailed eagle, great horned owl, and Humboldt penguin showed lower UGT-dependent activity than domestic birds. In synteny analysis, carnivorous birds were shown to have fewer UGT1E isoforms than herbivorous and omnivorous birds, which may explain why they have lower in vitro UGT activity. These observations suggested that raptors and seabirds, in which UGT activity is low, may be at high risk if exposed to elevated levels of xenobiotics in the environment. Phylogenetic analysis suggested that avian UGT1Es have evolved independently from mammalian UGT1As. We identified the important UGT isoforms, such as UGT1E13, and suspected their substrate specificities in avian xenobiotic metabolism by phylogenetic and quantitative real-time PCR analysis. This is the first report regarding the genetic characteristics and interspecies differences of UGT1Es in avian species.
  • Shouta M M Nakayama, Ayuko Morita, Yoshinori Ikenaka, Hazuki Mizukawa, Mayumi Ishizuka
    The Journal of veterinary medical science 81 2 298 - 313 2019年02月 [査読有り]
     研究論文(学術雑誌) 
    Worldwide use of anticoagulant rodenticides (ARs) for rodents control has frequently led to secondary poisoning of non-target animals, especially raptors. In spite of the occurrence of many incidents of primary or secondary AR-exposure and poisoning of non-target animals, these incidents have been reported only for individual countries, and there has been no comprehensive worldwide study or review. Furthermore, the AR exposure pathway in raptors has not yet been clearly identified. The aim of this review is therefore to comprehensively analyze the global incidence of primary and secondary AR-exposure in non-target animals, and to explore the exposure pathways. We reviewed the published literature, which reported AR residues in the non-target animals between 1998 and 2015, indicated that various raptor species had over 60% AR- detection rate and have a risk of AR poisoning. According to several papers studied on diets of raptor species, although rodents are the most common diets of raptors, some raptor species prey mainly on non-rodents. Therefore, preying on targeted rodents does not necessarily explain all causes of secondary AR-exposure of raptors. Since AR residue-detection was also reported in non-target mammals, birds, reptiles and invertebrates, which are the dominant prey of some raptors, AR residues in these animals, as well as in target rodents, could be the exposure source of ARs to raptors.
  • A. Shoji, K. H. Elliott, S. Aris-Brosou, H. Mizukawa, S. M.M. Nakayama, Y. Ikenaka, M. Ishizuka, T. Kuwae, K. Watanabe, J. Escoruela Gonzalez, Y. Watanuki
    Environmental Toxicology and Chemistry 38 1 106 - 114 2019年01月 [査読有り]
     研究論文(学術雑誌) 
    © 2018 SETAC Physical systems, such as currents and winds, have traditionally been considered responsible for transporting contaminants. Although evidence is mounting that animals play a role in this process through their movements, we still know little about how such contaminant biotransport occurs and the extent of effects at deposition sites. In the present study, we address this question by studying how rhinoceros auklets (Cerorhinca monocerata), a seabird that occurs in immense colonies (∼300 000 pairs at our study site, Teuri Island), affect contaminant levels at their colony and at nearby sites. More specifically, we hypothesize that contaminants are transported and deposited by seabirds at their colony and that these contaminants are passed on locally to the terrestrial ecosystem. To test this hypothesis, we analyzed the concentration of 9 heavy metal and metalloids, as well as δ 13 C and δ 15 N stable isotopes, in bird tissues, plants, and soil, both within and outside of the colony. The results show that rhinoceros auklets transport marine-derived mercury (Hg), possibly from their wintering location, and deposit Hg via their feces at their breeding site, thereby contaminating plants and soils within the breeding colony. The present study confirms not only that animals can transport contaminants from marine to terrestrial ecosystems, potentially over unexpectedly long distances, but also that bird tissues contribute locally to plant contamination. Environ Toxicol Chem 2019;38:106–114. © 2018 SETAC.
  • Aksorn Saengtienchai, Yoshinori Ikenaka, Minami Kawata, Yusuke Kawai, Kazuki Takeda, Takamitsu Kondo, Nesta Bortey-Sam, Shouta M M Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 214 28 - 35 2018年12月 [査読有り]
     研究論文(学術雑誌) 
    There have been many reports regarding toxic chemicals in birds. Chemicals are mainly metabolized in the liver through phase I oxidation by cytochrome P450 (CYP) and phase II conjugation by conjugated enzymes, such as UDP-glucuronosyltransferase (UGT), sulfotransferase (SULT), glutathione-S-transferase (GST), etc. Xenobiotic metabolism differs among bird species, but little detailed information is available. In the present study, the four-ring polycyclic aromatic hydrocarbon (PAH), pyrene, was used as a model xenobiotic to clarify the characteristics of xenobiotic metabolism in birds compared with laboratory animals by in vivo and in vitro studies. Plasma, bile, and excreta (urine and feces) were collected after oral administration of pyrene and analyzed to clarify xenobiotic metabolism ability in chickens and quails. Interestingly, pyrenediol-glucuronide sulfate (PYDOGS) and pyrenediol-diglucuronide (PYDOGG) were present in chickens and quails but not in rats. In addition, the area under the curve (AUC), maximum plasma concentration (Cmax), and time to maximum plasma concentration (Tmax) of pyrene-1-sulfate (PYOS) were higher than those of the parent molecule, pyrene, while the elimination half-life (t1/2) and mean residence time (MRT) were faster than those of the parent pyrene. With regard to sulfation of 1-hydroxypyrene (PYOH), the maximum velocity (Vmax) and Michaelis constant (Km) of rat liver cytosol were greater than those of chicken and quail liver cytosol. Furthermore, Vmax/Km of UGT activity in rat liver microsomes was also greater than those of chicken and quail liver microsomes. Characterization of xenobiotic metabolism revealed species differences between birds and mammals, raising concerns about exposure to various xenobiotics in the environment.
  • Chihiro Ishii, Shouta M M Nakayama, Andrew Kataba, Yoshinori Ikenaka, Keisuke Saito, Yukiko Watanabe, Yoshiki Makino, Takehisa Matsukawa, Ayano Kubota, Kazuhito Yokoyama, Hazuki Mizukawa, Takafumi Hirata, Mayumi Ishizuka
    Chemosphere 212 994 - 1001 2018年12月 [査読有り]
     研究論文(学術雑誌) 
    Lead (Pb) poisoning in raptors and water birds is a serious problem in many countries. However, only a small fraction of Pb poisoning has been detected in birds. Bone specimens may be useful indices of Pb exposure because bones contain ∼90% of the total Pb body burden. The original purpose of this study was to comprehensively analyze Pb accumulation in various bone types using inductively coupled plasma-mass spectrometry (ICP-MS). Since our results showed that Pb accumulation differed greatly depending on bone type, a secondary objective was defined, aiming to investigate the fine Pb distribution and its relation to bone structure and bone marrow by using laser ablation (LA)-ICP-MS. Our findings suggested that bone samples (1) consisting of trabecular tissue and (2) those that contain bone marrow could accumulate high levels of Pb following acute exposure. The shorter turnover time of trabecular bone can cause a rapid accumulation of Pb, and bone marrow may have an important role for internal exposure of Pb to bone tissue. Pb is accumulated in bones via blood flow, and bone marrow receives blood from outside the bones. In conclusion, bone samples provide valuable information on Pb exposure and could be useful to investigate and understand mortalities related to suspected Pb poisoning.
  • John Yabe, Shouta M M Nakayama, Yoshinori Ikenaka, Yared B Yohannes, Nesta Bortey-Sam, Abel Nketani Kabalo, John Ntapisha, Hazuki Mizukawa, Takashi Umemura, Mayumi Ishizuka
    Chemosphere 202 48 - 55 2018年07月 [査読有り]
     研究論文(学術雑誌) 
    Lead (Pb) and cadmium (Cd) are toxic metals that exist ubiquitously in the environment. Children in polluted areas are particularly vulnerable to metal exposure, where clinical signs and symptoms could be nonspecific. Absorbed metals are excreted primarily in urine and reflect exposure from all sources. We analyzed Pb and Cd concentrations in blood, feces and urine of children from polluted townships near a lead-zinc mine in Kabwe, Zambia, to determine concurrent childhood exposure to the metals. Moreover, the study determined the Pb and Cd relationships among urine, feces and blood as well as accessed the potential of urine and fecal analysis for biomonitoring of Pb and Cd exposure in children. Fecal Pb (up to 2252 mg/kg, dry weight) and urine Pb (up to 2914 μg/L) were extremely high. Concentrations of Cd in blood (Cd-B) of up to 7.7 μg/L, fecal (up to 4.49 mg/kg, dry weight) and urine (up to 18.1 μg/L) samples were elevated. metal levels were higher in younger children (0-3 years old) than older children (4-7). Positive correlations were recorded for Pb and Cd among blood, urine and fecal samples whereas negative correlations were recorded with age. These findings indicate children are exposed to both metals at their current home environment. Moreover, urine and feces could be useful for biomonitoring of metals due to their strong relationships with blood levels. There is need to conduct a clinical evaluation of the affected children to fully appreciate the health impact of these metal exposure.
  • Kazuki Takeda, Yoshinori Ikenaka, Kazuyuki D Tanaka, Shouta M M Nakayama, Tsutomu Tanikawa, Hazuki Mizukawa, Mayumi Ishizuka
    Pesticide biochemistry and physiology 148 42 - 49 2018年06月 [査読有り]
     研究論文(学術雑誌) 
    Anti-blood coagulation rodenticides, such as warfarin, have been used all over the world. They inhibit vitamin K epoxide reductase (VKOR), which is necessary for producing several blood clotting factors. This inhibition by rodenticides results in lethal hemorrhage in rodents. However, heavy usage of these agents has led to the appearance of rodenticide-resistant rats. There are two major mechanisms underlying this resistance, i.e., mutation of the target enzyme of warfarin, VKOR, and enhanced metabolism of warfarin. However, there have been few studies regarding the hepatic metabolism of warfarin, which should be related to resistance. To investigate warfarin metabolism in resistant rats, in situ liver perfusion of warfarin was performed with resistant black rats (Rattus rattus) from Tokyo, Japan. Liver perfusion is an in situ methodology that can reveal hepatic function specifically with natural composition of the liver. The results indicated enhanced hepatic warfarin hydroxylation activity compared with sensitive black rats. On the other hand, in an in vitro microsomal warfarin metabolism assay to investigate kinetic parameters of cytochrome P450, which plays a major role in warfarin hydroxylation, the Vmax of resistant rats was slightly but significantly higher compared to the results obtained in the in situ study. These results indicated that another factor like electron donators may also contribute to the enhanced metabolism in addition to high expression of cytochrome P450.
  • C Ishii, Y Ikenaka, O Ichii, S M M Nakayama, S-I Nishimura, T Ohashi, M Tanaka, H Mizukawa, M Ishizuka
    Poultry science 97 5 1722 - 1729 2018年05月 [査読有り]
     研究論文(学術雑誌) 
    Avian species have a unique renal structure and abundant blood flow into the kidneys. Although many birds die due to nephrotoxicity caused by chemicals, there are no early biomarkers for renal lesions. Uric acid level in blood, which is generally used as a renal biomarker, is altered when the kidney function is damaged by over 70%. Therefore, early biomarkers for kidney injury in birds are needed. In humans, glycomics has been at the forefront of biological and medical sciences, and glycans are used as biomarkers of diseases, such as carcinoma. In this study, a glycomics approach was used to screen for renal biomarkers in chicken. First, a chicken model of kidney damage was generated by injection of diclofenac or cisplatin, which cause acute interstitial nephritis (AIN) and acute tubular necrosis (ATN), respectively. The nephrotoxicity levels were determined by a blood chemical test and histopathological analysis. The plasma N-glycans were then analyzed to discover renal biomarkers in birds. Levels of 14 glycans increased between pre- and post administration in kidney-damaged chickens in the diclofenac group, and some of these glycans had the same presumptive composition as those in human renal carcinoma patients. Glycan levels did not change remarkably in the cisplatin group. It is possible that there are changes in glycan expression due to AIN, but they do not reflect ATN. Although further research is needed in other species of birds, glycans are potentially useful biomarkers for AIN in avian species.
  • L. A. Thompson, Y. Ikenaka, Y. B. Yohannes, T. Ichise, G. Ito, N. Bortey-Sam, J. J. van Vuren, V. Wepener, N. J. Smit, W. S. Darwish, S. M.M. Nakayama, H. Mizukawa, M. Ishizuka
    Bulletin of Environmental Contamination and Toxicology 100 5 672 - 676 2018年05月 [査読有り]
     研究論文(学術雑誌) 
    Many countries with incidence of malaria, including those surrounding Maputo Bay, use dichloro-diphenyl-trichloroethane (DDT) to reduce mosquitoes. This study is the first to estimate the human health risk associated with consumption of marine fish from Maputo Bay contaminated with DDTs. The median for ∑DDTs was 3.8 ng/g ww (maximum 280.9 ng/g ww). The overall hazard ratio for samples was 1.5 at the 75th percentile concentration and 28.2 at the 95th percentile. These calculations show increased potential cancer risks due to contamination by DDTs, data which will help policy makers perform a risk–benefit analysis of DDT use in malaria control programs in the region.
  • Emmanuel Temiotan Ogbomida, Shouta M M Nakayama, Nesta Bortey-Sam, Balazs Oroszlany, Isioma Tongo, Alex Ajeh Enuneku, Ogbeide Ozekeke, Martins Oshioriamhe Ainerua, Iriagbonse Priscillia Fasipe, Lawrence Ikechukwu Ezemonye, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    Ecotoxicology and environmental safety 151 98 - 108 2018年04月 [査読有り]
     研究論文(学術雑誌) 
    The use of free range animals for monitoring environmental health offers opportunities to detect exposure and assess the toxicological effects of pollutants in terrestrial ecosystems. Potential human health risk of dietary intake of metals and metalloid via consumption of offal and muscle of free range chicken, cattle and goats by the urban population in Benin City was evaluated. Muscle, gizzard, liver and kidney samples were analyzed for Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb concentrations using inductively coupled plasma mass spectrometer (ICP-MS) while Hg was determined using Hg analyzer. Mean concentrations of metals (mg/kg ww) varied significantly depending upon the tissues and animal species. Human health risk estimations for children and adults showed estimated daily intake (EDI) values of tissues below oral reference dose (RfD) threshold for non essential metals Cd, As, Pb and Hg thus strongly indicating no possible health risk via consumption of animal based food. Calculated Hazard quotient (THQ) was less than 1 (< 1) for all the metals analyzed for both adult and children. However, Cd and As had the highest value of THQ suggestive of possible health risk associated with continuous consumption of Cd and As contaminated animal based foods. Hazard Index (HI) for additive effect of metals was higher in chicken liver and gizzard for children and chicken liver for adults. Thus, HI indicated that chicken liver and gizzard may contribute significantly to adult and children dietary exposure to heavy metals. Principal component analysis (PCA) showed a clear species difference in metal accumulation between chickens and the ruminants. This study provides baseline data for future studies and also valuable evidence of anthropogenic impacts necessary to initiate national and international policies for control of heavy metal and metalloid content in food items.
  • R. Nakamura, A. Yabuki, O. Ichii, H. Mizukawa, N. Yokoyama, O. Yamato
    Journal of Comparative Pathology 160 79 - 83 2018年04月 [査読有り]
     研究論文(学術雑誌) 
    Renal capillary rarefaction is a crucial event that leads to tubulointerstitial damage during the progression of chronic kidney disease (CKD). In the present study, changes in CD34-positive renal capillaries were investigated in dogs and cats with CKD. A significant decrease in CD34-positive capillaries was observed in canine diseased kidneys, even at the early stage of disease. In cats, CD34-positive capillaries were well preserved in the diseased kidneys, with no link to the severity of CKD. Renal capillary rarefaction might be a trigger event that leads to the progression of CKD in dogs, rather than in cats.
  • Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M M Nakayama, Kwadwo A Asante, Elvis Baidoo, Christian Obirikorang, Hazuki Mizukawa, Mayumi Ishizuka
    Environmental pollution (Barking, Essex : 1987) 235 163 - 170 2018年04月 [査読有り]
     研究論文(学術雑誌) 
    Heavy metals and metalloids contamination in soils, water, food and livers of wild rats have been studied in Kumasi, Ghana and despite the estimated risks to residents, there is no epidemiological study to ascertain these projections. In addition, the World Health Organization and International Agency for Research on Cancer have reported an increase in respiratory diseases and cancers, in Ghana. The study's purpose was therefore to explore the potential associations between metal exposure and occurrences of respiratory diseases, lipid peroxidation and/or DNA damage to different age groups and sexes in Kumasi. Human urine was collected from the general population in urban and control sites in Kumasi and nine metals were measured in each sample. Results showed that although Zn was the most abundant total urinary As concentration was higher in 83% of samples compared to reference values. Urinary concentrations of metals, malondialdehyde (MDA) and 8-hydroxy-2-deoxy-guanosine (8-OHdG) were higher in urban sites compared to the control site. Based on the results obtained, there was no significant correlation between urinary metals and age. However, urinary Cd and MDA were highest in age groups 61-85 and 3-20 years, respectively. Significantly higher levels of urinary Co, As and Cd were detected in female participants. The study revealed that exposure to As was significantly associated with increased odds of asthma (odds ratio (OR) = 2.76; CI: 1.11-6.83) and tachycardia (OR = 3.93; CI: 1.01-15.4). Significant association was observed between urinary metals and MDA and 8-OHdG indicating possibility of lipid peroxidation and/or DNA damage in Kumasi residents.
  • Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M M Nakayama, Jemima T Marfo, Aksorn Saengtienchai, Hazuki Mizukawa, Mayumi Ishizuka
    The Journal of veterinary medical science 80 2 375 - 381 2018年03月 [査読有り]
     研究論文(学術雑誌) 
    Industrialization, economic and population growth rates in Ghana have increased the release of contaminants including polycyclic aromatic hydrocarbons (PAHs) into the environment through which humans and animals are exposed. Cattle is reported to be exposed to high levels of PAHs through feed and inhalation. Once exposed, PAHs are metabolized and excreted in urine, feces or bile. In a previous study, cattle in Ghana was reported to excrete high levels of 1-hydroxypyrene (1-OHPyr) due to high exposure to the parent compound, pyrene. 1-OHPyr is further metabolized to glucuronide and sulfate conjugates. Thus, the aim of this study was to investigate the sex and site differences in urinary excretion of conjugated pyrene metabolites using cattle urine collected from rural and urban sites of the Ashanti region, Ghana. From the results, geometric mean concentration adjusted by specific gravity indicated that 1-OHPyreneGlucuronide (PyG) was the most abundant conjugate followed by PyrenediolSulfate (M3). The sum of conjugated pyrene metabolites and sum of both conjugated and deconjugated pyrene metabolites correlated significantly with PyG, PydiolSulfate (M2) and PydiolSulfate (M3). The study revealed no significant difference in urinary excretion of conjugated pyrene metabolites between rural and urban sites. This indicated that similar to urban sites, cattle in rural sites were exposed to high levels of pyrene. There was no significant difference in urinary concentrations of conjugated pyrene metabolites between sexes.
  • Darwish WS, Ikenaka Y, Nakayama S, Mizukawa H, Thompson LA, Ishizuka M
    Environmental science and pollution research international 25 7 6320 - 6328 2018年03月 [査読有り]
     研究論文(学術雑誌) 
    Benzo[a]pyrene (B[a]P) is one of the polycyclic aromatic hydrocarbons which is formed due to smoking of foods, incomplete combustion of woods, vehicle exhausts, and cigarettes smokes. B[a]P gets entry into human and animal bodies mainly through their diets. Metabolic activation of B[a]P is required to induce mutagenesis and carcinogenesis in animal and human studies. Carotenoids and retinoids are phytochemicals that if ingested have multiple physiological interferences in the human and animal bodies. In this study, we firstly investigated the protective effects of β-carotene, β-apo-8-carotenal, retinol, and retinoic acid against B[a]P-induced mutagenicity and oxidative stress in human HepG2 cells. Secondly, we tested the hypothesis of modulating xenobiotic metabolizing enzymes (XMEs) by carotenoids and retinoids as a possible mechanism of protection by these micronutrients against B[a]P adverse effects. The obtained results declared that β-carotene and retinol significantly reduced B[a]P-induced mutagenicity and oxidative stress. Tested carotenoids and retinoids reduced B[a]P-induced phase I XMEs and induced B[a]P reduced phase II and III XMEs. Thus, the protective effects of these micronutrients are probably due to their ability of induction of phase II and III enzymes and interference with the induction of phase I enzymes by the promutagen, B[a]P. It is highly recommended to consume foods rich in these micronutrients in the areas of high PAH pollution.
  • Daisuke Ueno, Hazuki Mizukawa, Osamu Inanami, Hiromitsu Nagasaka, Nozomi Tatsuta, Yukinori Narazaki, Takeshi Fujino, Izumi Watanabe, Yutaka Kameda, Kunihiko Nakai
    Landscape and Ecological Engineering 14 1 29 - 35 2018年01月 [査読有り]
     研究論文(学術雑誌) 
    The “Caddisfly Watch” program proposes the use of larvae of the caddisfly genus Stenopsyche (Trichoptera: Stenopsychidae) to monitor the radioactive cesium (137Cs) pollution, including that of suspended solids, in river environments, as a simple method was essential for this following the Fukushima nuclear disaster in March 2011. A variety of aquatic organisms were collected from rivers in Japan in 2012 and their levels of radioactive Cs measured. Amongst all the organisms collected, the highest concentrations of 137Cs were observed in caddisfly larvae. These larvae occur at a high density and can be collected at regular intervals in most rivers throughout Japan. It is proposed that caddisfly larvae can be used as bioindicators of radioactive Cs contamination in rivers, as their temporal and spatial changes are easily assessed.
  • Yoshinori Ikenaka, Kazutoshi Fujioka, Tomonori Kawakami, Takahiro Ichise, Nesta Bortey-Sam, Shouta M M Nakayama, Hazuki Mizukawa, Kumiko Taira, Keisuke Takahashi, Keisuke Kato, Koji Arizono, Mayumi Ishizuka
    Toxicology reports 5 744 - 749 2018年 [査読有り]
     研究論文(学術雑誌) 
    Tea is one of the world's most popular beverages due to health promoting effects. Despite these, there have been concerns about the adverse effects of tea contamination by neonicotinoid insecticides. Only a handful of studies on neonicotinoid insecticides in tea have been carried out and this study was therefore performed to determine the concentrations of seven neonicotinoid insecticides and 20 metabolites in Japanese green tea leaves, and black tea leaves from Sri Lanka; and assess the Maximum Daily Intake (MDI) of neonicotinoid insecticides. From the results, the seven parent compounds were detected in Japanese tea leaves and beverages. Dinotefuran (3004 ng/g) was found at the highest level in green tea leaves. Ten of the 20 metabolites were detected in Japanese tea products. Dinotefuran-urea (92%) and thiacloprid-amide (89%) were most frequently detected in Japanese tea leaves. Clothianidin-urea (100 ng/g) was found at the highest level in green tea leaves. Neonicotinoid insecticides and metabolites were not detected in Sri Lankan black tea leaves. The concentrations and MDI of neonicotinoid insecticides in tea leaves were below the Maximum Residual Levels (MRLs) and Acceptable Daily Intakes (ADIs), respectively.
  • Lesa A Thompson, Yoshinori Ikenaka, Wageh S Darwish, Yared B Yohannes, Johan J van Vuren, Victor Wepener, Nico J Smit, Atnafu G Assefa, Ahmed Tharwat, Walaa Fathy Saad Eldin, Shouta M M Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    PloS one 13 10 e0204400  2018年 [査読有り]
     研究論文(学術雑誌) 
    The objective of this study was to identify potential mRNA expression changes in chicken livers associated with environmental exposure to dichloro-diphenyl-trichloroethane (DDT) and its metabolites (DDTs). In particular, we focused on genes relating to the immune system and metabolism. We analyzed liver samples from free-ranging chickens in KwaZulu-Natal, South Africa, for contamination by DDTs. This area predominantly uses DDT in its malaria control program, and homes are sprayed annually with the pesticide. Genes relating to the immune system and metabolism were selected as potential genetic biomarkers that could be linked to higher contamination with DDTs. RT-qPCR analysis on 39 samples showed strong correlations between DDTs contamination and mRNA expression for the following genes: AvBD1, AvBD2, AvBD6 and AvBD7 (down-regulated), and CYP17, ELOVL2 and SQLE (up-regulated). This study shows for the first time interesting and significant correlations between genetic material collected from environmentally-exposed chickens and mRNA expression of several genes involved in immunity and metabolism. These findings show the usefulness of analysis on field samples from a region with high levels of environmental contamination in detecting potential biomarkers of exposure. In particular, we observed clear effects from DDT contamination on mRNA expression of genes involved in immune suppression, endocrine-disrupting effects, and lipid dysregulation. These results are of interest in guiding future studies to further elucidate the pathways involved in and clinical importance of toxicity associated with DDT exposure from contaminated environments, to ascertain the health risk to livestock and any subsequent risks to food security for people.
  • ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響
    水川 葉月, 高居 名菜, 野見山 桂, 高口 倖暉, 横山 望, 市居 修, 滝口 満喜, 池中 良徳, 中山 翔太, 石塚 真由美
    環境ホルモン学会研究発表会要旨集 20回 78 - 78 環境ホルモン学会(日本内分泌撹乱化学物質学会) 2017年12月 [査読有り]
  • Chihiro Ishii, Shouta M. M. Nakayama, Yoshinori Ikenaka, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Hazuki Mizukawa, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Mayumi Ishizuka
    CHEMOSPHERE 186 367 - 373 2017年11月 [査読有り]
     研究論文(学術雑誌) 
    Lead (Pb) poisoning is widespread among raptors and water birds. In Japan, fragments of Pb ammunition are still found in endangered eagles although more than 10 years have passed since legislation regarding use of Pb ammunition was introduced. This study was performed to investigate Pb exposure in raptors from various locations in Japan. We measured hepatic and renal Pb concentrations and hepatic Pb isotope ratios of Steller's sea eagles (Haliaeetus pelagicus), white-tailed sea eagles (Haliaeetus albicilla), golden eagles (Aquila chrysaetos), and 13 other species (total 177 individuals) that were found dead, as well as blood samples from three eagles found in a weakened state during 1993-2015 from Hokkaido (northern part), Honshu (the main island), and Shikoku (a southern island) of japan. In the present study in Hokkaido, one quarter of the sea eagles showed a high Pb concentration, suggesting exposure to abnormally high Pb levels and Pb poisoning. Pb isotope ratios indicated that endangered Steller's sea eagle and white-tailed sea eagle were poisoned by Pb ammunition that was used illegally in Hokkaido. In other areas of Japan, both surveillance and regulations were less extensive than in Hokkaido, but Pb poisoning in raptors was also noted. Therefore, Pb poisoning is still a serious problem in raptors in various areas of Japan due to accidental ingestion of materials containing Pb, especially Pb ammunition. (C) 2017 Published by Elsevier Ltd.
  • Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Miyuki Yamamoto, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 51 19 11354 - 11362 2017年10月 [査読有り]
     研究論文(学術雑誌) 
    Present study determined concentrations and residue patterns of bromophenols (BPhs) in whole blood samples of pet cats and pet dogs collected from veterinary hospitals in Japan. BPhs concentrations were higher in cat blood than in dog blood, with statistically insignificant differences (p = 0.07). Among the congeners, 2,4,6-tribromophenol (TBPh) constituted the majority of BPhs (>90%) detected in both species. Analysis of commercial pet food to estimate exposure routes showed that the most abundant congener in all pet food samples was 2,4,6-TBPh, accounting for >99% of total BPhs. This profile is quite similar to the blood samples of the pets, suggesting that diet might be an important exposure route for BPhs in pets. After incubation in polybrominated diphenyl ether (PBDE) mixtures (BDE-47, BDE-99 and BDE-209), 2,4,5-TBPh was found in dog liver microsomes but not in cat liver microsomes, implying species-specific metabolic capacities for PBDEs. Formation of 2,4,5-TBPh occurred by hydroxylation at the 1' carbon atom of the ether bond of BDE-99 is similar to human study reported previously. Hydroxylated PBDEs were not detected in cats or dogs; therefore, diphenyl ether bond cleavage of PBDEs can also be an important metabolic pathway for BPhs formation in cats and dogs.
  • Yared Beyene Yohannes, Yoshinori Ikenaka, Gengo Ito, Shouta M. M. Nakayama, Hazuki Mizukawa, Victor Wepener, Nico J. Smit, Johan H. J. Van Vuren, Mayumi Ishizuka
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 24 30 23763 - 23770 2017年10月 [査読有り]
     研究論文(学術雑誌) 
    Ethiopia and South Africa are among the few countries to still implement indoor residual spraying with dichloro-diphenyl-trichloroethane (DDT) for malaria vector control. In this study, we investigated the levels and ecological risks of DDT and its metabolites in liver tissues of house rat, as a sentinel animal, for providing an early warning system for public health and wildlife intervention from Ethiopia and South Africa. The results showed that Sigma DDT concentration ranged from 127 to 9155 mu g/kg wet weight, and the distribution order of DDT and its metabolites in the analyzed liver samples was p,p'-DDD > p,p'-DDE >> p,p'-DDT, o,p'-DDT, and o,p'-DDD. The risk assessment indicated a potential adverse impact on humans, especially for pregnant women and children, because they spend majority of their time in a DDT-sprayed house. The ecological assessment also showed a concern for birds of prey and amphibians like frogs. This study is the first report on DDT contamination in liver tissues of house rats from Ethiopia and South Africa, and henceforth, the data will serve as a reference data for future studies.
  • Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M. M. Nakayama, Kwadwo A. Asante, Elvis Baidoo, Christian Obirikorang, Aksorn Saengtienchai, Norikazu Isoda, Collins Nimako, Hazuki Mizukawa, Mayumi Ishizuka
    ENVIRONMENTAL POLLUTION 228 311 - 320 2017年09月 [査読有り]
     研究論文(学術雑誌) 
    Studies of polycyclic aromatic hydrocarbons (PAHs) and its metabolites in PM10, soils, rat livers and cattle urine in Kumasi, Ghana, revealed high concentrations and cancer potency. In addition, WHO and IARC have reported an increase in cancer incidence and respiratory diseases in Ghana. Human urine were therefore collected from urban and control sites to: assess the health effects associated with PAHs exposure using malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG); identify any association between OH-PAHs, MDA, 8-OHdG with age and sex; and determine the relationship between PAHs exposure and occurrence of respiratory diseases. From the results, urinary concentrations of the sum of OH-PAHs (Sigma OHPAHs) were significantly higher from urban sites compared to the control site. Geometric mean concentrations adjusted by specific gravity, GM(SG), indicated 2-OHNaphthalene (2-OHNap) (6.01 +/- 4.21 ng/mL) as the most abundant OH-PAH, and exposure could be through the use of naphthalene-containing-mothballs in drinking water purification, insect repellent, freshener in clothes and/or "treatment of various ailments". The study revealed that exposure to naphthalene significantly increases the occurrence of persistent cough (OR = 2.68, CI: 1.43-5.05), persistent headache (OR = 1.82, CI: 1.02-3.26), tachycardia (OR = 3.36, CI: 1.39-8.10) and dyspnea (OR = 3.07, CI: 1.27-7.43) in Kumasi residents. Highest level of urinary 2-OHNap (224 ng/mL) was detected in a female, who reported symptoms of persistent cough, headache, tachycardia, nasal congestion and inflammation, all of which are symptoms of naphthalene exposure according to USEPA. The Sigma OHPAHs, 2-OHNap, 2-3-OHFluorenes, and -OHPhenanthrenes showed a significantly positive correlation with MDA and 4-OHPhenanthrene with 8-OHdG, indicating possible lipid peroxidation/cell damage or degenerative disease in some participants. MDA and 8-OHdG were highest in age group 21-60. The present study showed a significant sex difference with higher levels of urinary OH-PAHs in females than males. (C) 2017 Published by Elsevier Ltd.
  • Takamitsu Kondo, Yoshinori Ikenaka, Shouta M. M. Nakayama, Yusuke K. Kawai, Hazuki Mizukawa, Yoko Mitani, Kei Nomiyama, Shinsuke Tanabe, Mayumi Ishizuka
    TOXICOLOGICAL SCIENCES 158 1 90 - 100 2017年07月 [査読有り]
     研究論文(学術雑誌) 
    UDP-glucuronosyltransferases (UGTs) are among the most important xenobiotic metabolizing enzymes that conjugate a wide range of chemicals. Previous studies showed that Felidae and Pinnipedia species have very low UGT activities toward some phenolic compounds because of the UGT1A6 pseudogene and small numbers of UGT1A isozymes. In addition to the UGT1As, UGT2Bs isozymes also conjugate various endogenous (eg, estrogens, androgens, and bile acids) and exogenous compounds (opioids, non-steroidal anti-inflammatory drugs, and environmental pollutants). However UGT2B activity and genetic background are unknown in carnivore species. Therefore, this study was performed to elucidate the species differences of UGT2Bs. Using typical substrates for UGT2Bs, UGT activity was measured in vitro. In addition, UGT2B genetic features are analyzed in silico. Results of UGT activity measurement indicate marked species differences between dogs and other carnivores (cats, Northern fur seals, Steller sea lions, Harbor seals, and Caspian seals). Dogs have very high V-max/K-m toward estradiol (17-glucuronide), estrone, lorazepam, oxazepam, and temazepam. Conversely, cats and pinniped species (especially Caspian seals and Harbor seals) have very low activities toward these substrates. The results of genetic synteny analysis indicate that Felidae and pinniped species have very small numbers of UGT2B isozymes (one or none) compared with dogs, rodents, and humans. Furthermore, Felidae species have the same nonsense mutation in UGT2B, which suggests that Felidae UGT2B31-like is also a pseudogene in addition to UGT1A6. These findings of lower activity of UGT2B suggest that Felidae and some pinniped species have very low UGT activity toward a wide range of chemicals. These results are important for Felidae and Pinnipedia species that are frequently exposed to drugs and environmental pollutants.
  • Kei Nomiyama, Kohki Takaguchi, Hazuki Mizukawa, Yasuko Nagano, Tomoko Oshihoi, Susumu Nakatsu, Tatsuya Kunisue, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 51 10 5811 - 5819 2017年05月 [査読有り]
     研究論文(学術雑誌) 
    The adverse effects of elevated polybrominated diphenyl ether (PBDE) levels, reported in the blood of domestic dogs and cats, are considered to be of great concern. However, the tissue distribution of PBDEs and their derivatives in these animals is poorly understood. This study determined the concentrations and profiles of PBDEs, hydroxylated PBDEs (OH-PBDEs), methoxylated PBDEs (MeO-PBDEs), and 2,4,6-tribromophenol (2,4,6-tri-BPh) in the blood, livers, bile, and brains of dogs and cats in Japan. Higher tissue concentrations of PBDEs were found in cats, with the dominant congener being BDE209. BDE207 was also predominant in cat tissues, indicating that BDE207 was formed via BDE209 debromination. BDE47 was the dominant congener in dog bile, implying a species-specific excretory capacity of the liver. OH-PBDE and MeO-PBDE concentrations were several orders of magnitude higher in cat tissues, with the dominant congener being 6OH-BDE47, possibly owing to their intake of naturally occurring MeO-PBDEs in food, MeO-PBDE demethylation in the liver, and lack of UDP-glucuronosyltransferase, UGT1A6. Relatively high concentrations of BDE209, BDE207, 6OH-BDE47, 2'MeO-BDE68, and 2,4,6-tri-BPh were found in cat brains, suggesting a passage through the blood brain barrier. Thus, cats in Japan might be at a high risk from PBDEs and their derivatives, particularly BDE209 and 6OH-BDE47.
  • Lesa A. Thompson, Wageh Sobhy Darwish, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    JOURNAL OF VETERINARY MEDICAL SCIENCE 79 4 751 - 764 2017年04月 [査読有り]
     
    Organochlorine pesticides (OCPs) have been used worldwide, particularly in Africa, for several decades. Although many are banned, several African countries still use OCPs especially for the prevention and control of malaria. OCPs are characterized by their bio-accumulation in the environment, especially in the food chain, where they find their way into the human body. Despite no clear epidemiological studies confirming hazardous effects of these chemicals on human health, many studies have reported positive associations between the use of OCPs and neurological and reproductive disorders, and cancer risk. There is a clear gap in published reports on OCPs in Africa and their potential health hazards. Thus, the aim of this review is to summarize the incidence of OCP contamination in various foods in Africa, to demonstrate the potential transmission of these chemicals to people and to discuss their possible health hazards.
  • Chihiro Ishii, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Yared Beyene Yohannes, Yutaka Watanuki, Masaaki Fukuwaka, Mayumi Ishizuka
    JOURNAL OF VETERINARY MEDICAL SCIENCE 79 4 807 - 814 2017年04月 [査読有り]
     研究論文(学術雑誌) 
    Seabirds are marine top predators and accumulate high levels of metals and metalloids in their tissues. Contamination by metals in the highly productive offshore region has become a matter of public concern. It is home to 80% of the seabird population in the U.S.A., 95% of northern fur seals (Callorhinus ursinus), and major populations of Steller sea lions (Eumetopias jubatus), walruses (Odobenus rosmarus) and whales. Here, the concentrations of eight heavy metals (Hg, Cd, Cr, Co, Ni, Cu, Zn and Pb) and a metalloid (As) in the liver and kidneys of the northern fulmar (Fulmarus glacialis), thick-billed murre (Uria lomvia), short-tailed shearwater (Puffinus tenuirostris), tufted puffin (Fratercula cirrhata) and horned puffin (Fratercula corniculata) collected in the Bering Sea were measured. As proxies of trophic level and habitat, nitrogen (delta N-15) and carbon (delta C-13) stable isotope ratios of breast muscles were also measured. Hepatic Hg concentration was high in northern fulmar, whereas Cd level was high in tufted puffin and northern fulmar. The Hg concentration and d15N value were positively correlated across individual birds, suggesting that Hg uptake was linked to the trophic status of consumed prey. Furthermore, Hg concentration in our study was higher than those of the same species of seabirds collected in 1990.
  • Hazuki Mizukawa, Yoshinori Ikenaka, Mayu Kakehi, Shouta Nakayama, Mayumi Ishizuka
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN 137 3 257 - 263 2017年03月 [査読有り]
     
    The ability to metabolize xenobiotics in organisms has a wide degree of variation among organisms. This is caused by differences in the pattern of xenobiotic bioaccumulation among organisms, which affects their tolerance. It has been reported in the veterinary field that glucuronidation (UGT) activity in cats, acetylation activity in dogs and sulfation (SULT) activity in pigs are sub-vital in these species, respectively, and require close attention when prescribing the medicine. On the other hand, information about species differences in xenobiotics metabolism remains insufficient, especially in non-experimental animals. In the present study, we tried to elucidate xenobiotic metabolism ability, especially in phase II UGT conjugation of various non-experimental animals, by using newly constructed in vivo, in vitro and genomic techniques. The results indicated that marine mammals (Steller sea lion, northern fur seal, and Caspian seal) showed UGT activity as low as that in cats, which was significantly lower than in rats and dogs. Furthermore, UGT1A6 pseudogenes were found in the Steller sea lion and Northern fur seal; all Otariidae species are thought to have the UGT1A6 pseudogene as well. Environmental pollutants and drugs conjugated by UGT are increasing dramatically in the modern world, and their dispersal into the environment can be of great consequence to Carnivora species, whose low xenobiotic glucuronidation capacity makes them highly sensitive to these compounds.
  • Yared Beyene Yohannes, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 98 2 172 - 177 2017年02月 [査読有り]
     研究論文(学術雑誌) 
    Concentrations of ten trace elements (Hg, As, Cd, Pb, Co, Cr, Cu, Ni, Se and Zn) were determined in different tissues (liver, kidney, muscle, heart and brain) of African sacred ibis (Threskiornis aethiopicus), Hamerkop (Scopus umbretta), marabou stork (Leptoptilos crumeniferus) and great white pelican (Pelecanus onocrotalus) inhabiting the Ethiopian Rift Valley region. There were differences in trace element patterns among the bird species. Significantly (p < 0.05) higher concentrations of Cd (5.53 A mu g/g dw +/- 2.94) in kidney and Hg (0.75 A mu g/g ww +/- 0.30) in liver were observed in the great white pelican compared to the other species, and liver concentrations of these two elements showed positive correlations with trophic level. Concentrations of toxic elements (As, Cd, Pb and Hg) in liver were below their respective toxicological thresholds, indicating that the data may provide baseline information for future studies.
  • Hokuto Nakata, Shouta M M Nakayama, Balazs Oroszlany, Yoshinori Ikenaka, Hazuki Mizukawa, Kazuyuki Tanaka, Tsunehito Harunari, Tsutomu Tanikawa, Wageh Sobhy Darwish, Yared B Yohannes, Aksorn Saengtienchai, Mayumi Ishizuka
    International journal of environmental research and public health 14 1 2017年01月 [査読有り]
     研究論文(学術雑誌) 
    Although Japan has been considered to have little lead (Pb) pollution in modern times, the actual pollution situation is unclear. The present study aims to investigate the extent of Pb pollution and to identify the pollution sources in Japan using stable Pb isotope analysis with kidneys of wild rats. Wild brown (Rattus norvegicus, n = 43) and black (R. rattus, n = 98) rats were trapped from various sites in Japan. Mean Pb concentrations in the kidneys of rats from Okinawa (15.58 mg/kg, dry weight), Aichi (10.83), Niigata (10.62), Fukuoka (8.09), Ibaraki (5.06), Kyoto (4.58), Osaka (4.57), Kanagawa (3.42), and Tokyo (3.40) were above the threshold (2.50) for histological kidney changes. Similarly, compared with the previous report, it was regarded that even structural and functional kidney damage as well as neurotoxicity have spread among rats in Japan. Additionally, the possibility of human exposure to a high level of Pb was assumed. In regard to stable Pb isotope analysis, distinctive values of stable Pb isotope ratios (Pb-IRs) were detected in some kidney samples with Pb levels above 5.0 mg/kg. This result indicated that composite factors are involved in Pb pollution. However, the identification of a concrete pollution source has not been accomplished due to limited differences among previously reported values of Pb isotope composition in circulating Pb products. Namely, the current study established the limit of Pb isotope analysis for source identification. Further detailed research about monitoring Pb pollution in Japan and the demonstration of a novel method to identify Pb sources are needed.
  • Hokuto Nakata, Shouta M. M. Nakayama, Balazs Oroszlany, Yoshinori Ikenaka, Hazuki Mizukawa, Kazuyuki Tanaka, Tsunehito Harunari, Tsutomu Tanikawa, Wageh Sobhy Darwish, Yared B. Yohannes, Aksorn Saengtienchai, Mayumi Ishizuka
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 14 1 2017年01月 [査読有り]
     研究論文(学術雑誌) 
    Although Japan has been considered to have little lead (Pb) pollution in modern times, the actual pollution situation is unclear. The present study aims to investigate the extent of Pb pollution and to identify the pollution sources in Japan using stable Pb isotope analysis with kidneys of wild rats. Wild brown (Rattus norvegicus, n = 43) and black (R. rattus, n = 98) rats were trapped from various sites in Japan. Mean Pb concentrations in the kidneys of rats from Okinawa (15.58 mg/kg, dry weight), Aichi (10.83), Niigata (10.62), Fukuoka (8.09), Ibaraki (5.06), Kyoto (4.58), Osaka (4.57), Kanagawa (3.42), and Tokyo (3.40) were above the threshold (2.50) for histological kidney changes. Similarly, compared with the previous report, it was regarded that even structural and functional kidney damage as well as neurotoxicity have spread among rats in Japan. Additionally, the possibility of human exposure to a high level of Pb was assumed. In regard to stable Pb isotope analysis, distinctive values of stable Pb isotope ratios (Pb-IRs) were detected in some kidney samples with Pb levels above 5.0 mg/kg. This result indicated that composite factors are involved in Pb pollution. However, the identification of a concrete pollution source has not been accomplished due to limited differences among previously reported values of Pb isotope composition in circulating Pb products. Namely, the current study established the limit of Pb isotope analysis for source identification. Further detailed research about monitoring Pb pollution in Japan and the demonstration of a novel method to identify Pb sources are needed.
  • Yared Beyene Yohannes, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    SCIENCE OF THE TOTAL ENVIRONMENT 574 1389 - 1395 2017年01月 [査読有り]
     研究論文(学術雑誌) 
    Despite the presence of a wide variety and number of birds, there is exceedingly little data on organochlorine pesticide (OCP) residues in birds inhabiting in Africa. In the present study, concentrations of dichlorodiphenyl-trichloroethanes (DDTs), hexachlorocyclohexanes, drins, cyclodienes, and hexachlorobenzene were measured in liver, kidney, heart and brain of 4 bird species from the Rift Valley region, Ethiopia. Indoor residual spraying of DDT for malaria vector control, and indiscriminate and illegal use of pesticides underline the relevance of this study. Levels of Sigma OCPs ranged from 1.87 to 4586 ng/g wet weight, and the scavenger bird species Leptoptilos crumeniferus had the highest level in liver. In all tissues, contamination profiles of OCPs within the species were similar, with DDTs >> other OCPs. Among the DDTs, p,p'-DDE was the most abundant compound and had significantly a higher burden in all tissues. The risk characterization demonstrated potential risks to the studied birds associated with DDE exposure. Maximum hepatic levels of p,p'-DDE exceeded the levels reported to trigger adverse effects. The detection of p,p'-DDT in all bird tissues suggests the release of fresh DDT to the environment. This is the first study to assay OCPs in different tissues of birds from the Ethiopian Rift Valley region, and henceforth the data will serve as a reference data for future studies. (C) 2016 Elsevier B.V. All rights reserved.
  • Aksorn Saengtienchai, Yoshinori Ikenaka, Nesta Bortey-Sam, Usuma Jermnark, Hazuki Mizukawa, Yusuke K. Kawai, Shouta M. M. Nakayama, Mayumi Ishizuka
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 190 38 - 47 2016年12月 [査読有り]
     研究論文(学術雑誌) 
    The African hedgehog, Atelerix albiventris, is a spiny mammal that has become popular as an exotic pet in many countries. To elucidate the ability of hedgehogs to metabolize xenobiotics, the animals were exposed to polycyclic aromatic hydrocarbon, pyrene. The in vivo exposure study indicated that pyrene was biotransformed to glucuronide and sulfate conjugates, such as pyrene-l-glucuronide, pyrene-1-sulfate, and pyrenediol-sulfate, and excreted in the urine. Pyrene-l-glucuronide was the main metabolite, and limited sulfate conjugate excretion was observed. The main products excreted in feces were 1-hydroxypyrene and pyrene. Based on the results of the in vivo exposure study, in vitro enzymatic kinetic experiments were performed using various substrates and compared to rats and pigs. The enzyme efficiencies of cytochrome P450 (CYP)-mediated ethoxyresorufin 0-deethylase activity and warfarin 4 '-, 6-, and 8-hydroxylation activity in hedgehogs were lower than those of rats. Furthermore, UDP-glucuronosyltransferase activity in hedgehogs also had a lower Km value than that in pigs. Interestingly, the enzyme efficiencies of sulfation activity toward 1-hydroxypyrene and beta-estradiol in hedgehogs were significantly lower than those in pigs. These observations suggested that phenol and estrogen sulfotransferases may have limited roles in xenobiotic metabolism in hedgehogs. (C) 2016 Elsevier Inc. All rights reserved.
  • Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M. M. Nakayama, Jemima Marfo, Aksorn Saengtienchai, Hazuki Mizukawa, Mayumi Ishizuka
    ENVIRONMENTAL POLLUTION 218 331 - 337 2016年11月 [査読有り]
     研究論文(学術雑誌) 
    Previous studies of polycyclic aromatic hydrocarbons (PAHs) in particulate matter, soils and livers of wild rats indicated that the city centre of Kumasi, Ghana has been severely polluted with high cancer potency. Cattle urine were therefore collected from Kumasi (urban) and Offinso (rural), Ghana: to determine concentrations of urinary PAH metabolites (OH-PAHs); and find their association with sex; and to estimate exposure of cattle to PAHs from the different sites. From the results, geometric mean concentrations (adjusted by specific gravity), GM(SG), showed that 2-OHNaphthalene (2-OHNap) was the most abundant OH-PAH in cattle urine from all study sites, and naphthalene-containing-mothballs might have contributed significantly to the levels. There was no significant difference between urinary OH-PAHs concentrations in cattle from urban and rural sites except for 2-OHPhe and 4-OHPhe, and similar to urban areas, rural sites could also be polluted with PAHs. GMSG of 2-OHNap in cattle urine in Kokote (21.9 +/- 6.51 ng/mL; a rural area), was significantly higher compared to the other sites followed by Oforikrom (4.15 +/- 4.37 ng/mL; urban). The GMSG concentration (ng/mL) of the sum of OH-PAHs decreased in the order, Kokote (44.7) > Oforikrom (7.87) > Saboa (6.98) > Santasi (6.68) > and Twumasen Estate (5.23). The high concentrations of urinary 2-OHNap, 2-OHPhe, 3-OHPhe and 4-OHPhe in Kokote indicated high PAHs exposure to cattle in this area or different/specific source of PAHs exposure. GMSG of 2-OHNap was significantly higher in male cattle compared to females while 1-9-OHPhe was significantly higher in females. (C) 2016 Published by Elsevier Ltd.
  • Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 134 1 - 7 2016年11月 [査読有り]
     研究論文(学術雑誌) 
    Roof rats (Rattus rattus) live mainly in human habitats. Heavy use of rodenticides, such as warfarin, has led to the development of drug resistance, making pest control difficult. There have been many reports regarding mutations of vitamin K epoxide reductase (VKOR), the target enzyme of warfarin, in resistant rats. However, it has been suggested there are other mechanisms of warfarin resistance. To confirm these possibilities, closed colonies of warfarin-susceptible roof rats (S) and resistant rats from Tokyo (R) were established, and the pharmacokinetics/ pharmacodynamics of warfarin in rats from both colonies was investigated. R rats had low levels of warfarin in serum and high clearance activity. These rats can rapidly metabolize warfarin by hydroxylation. The levels of accumulation in the organs were lower than those of S rats. R rats administered warfarin showed high expression levels of CYP2B, 2C, and 3A, which play roles in warfarin hydroxylation, and may explain the high clearance ability of R rats. The mechanism of warfarin resistance in roof rats from Tokyo involved not only mutation of VKOR but also high clearance ability due to high levels of CYP2B, 2C and 3A expression possibly induced by warfarin. (C) 2016 Elsevier Inc. All rights reserved.
  • S. M. M. Nakayama, Y. Ikenaka, A. Hayami, H. Mizukawa, W. S. Darwish, K. P. Watanabe, Y. K. Kawai, M. Ishizuka
    JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 39 5 478 - 487 2016年10月 [査読有り]
     研究論文(学術雑誌) 
    Research on drug metabolism and pharmacokinetics in large animal species including the horse is scarce because of the challenges in conducting in vivo studies. The metabolic reactions catalyzed by cytochrome P450s (CYPs) are central to drug pharmacokinetics. This study elucidated the characteristics of equine CYPs using diazepam (DZP) as a model compound as this drug is widely used as an anesthetic and sedative in horses, and is principally metabolized by CYPs. Diazepam metabolic activities were measured in vitro using horse and rat liver microsomes to clarify the species differences in enzyme kinetic parameters of each metabolite (temazepam [TMZ], nordiazepam [NDZ], p-hydroxydiazepam [p-OH-DZP], and oxazepam [OXZ]). In both species microsomes, TMZ was the major metabolite, but the formation rate of p-OH-DZP was significantly less in the horse. Inhibition assays with a CYP-specific inhibitors and antibody suggested that CYP3A was the main enzyme responsible for DZP metabolism in horse. Four recombinant equine CYP3A isoforms expressed in Cos-7 cells showed that CYP3A96, CYP3A94, and CYP3A89 were important for TMZ formation, whereas CYP3A97 exhibited more limited activity. Phylogenetic analysis suggested diversification of CYP3As in each mammalian order. Further study is needed to elucidate functional characteristics of each equine CYP3A isoform for effective use of diazepam in horses.
  • Abdallah Fikry A. Mahmoud, Yoshinori Ikenaka, Yared B. Yohannes, Wageh S. Darwish, Elsaid A. Eldaly, Alaa Eldin M. A. Morshdy, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    CHEMOSPHERE 144 1365 - 1371 2016年02月 [査読有り]
     研究論文(学術雑誌) 
    Food consumption is an important route of human exposure to organochlorine pesticides (OCPs). In order to assess the potential human health risks associated with OCPs, edible cattle tissues (liver, kidney and tongue) were collected from three slaughter houses in Mansoura, Zagazig and Ismailia cities, Egypt. Levels of 22 OCPs such as hexachlorocyclohexanes (HCHs), dichlorodiphenyltrichloroethanes (DDTs), aldrin, dieldrin and endrin (Drins), chlordanes (CHLs), heptachlors (HPTs) and hexachlorobenzene (HCB) residues were investigated. Among the investigated OCPs, HCHs represented the most dominant group with high proportions of gamma-HCH isomer (53-91% of total HCHs). Mansoura city had the highest OCPs contamination load ranged from 0.1 to 2827 ng g(-1) lw (lipid weight). Surprisingly, tongue samples collected from Mansoura showed the highest concentration of HCHs (448 ng g(-1) lw) in comparison to liver (152 ng g(-1) lw) and kidney (266 ng g(-1) lw). Generally, contamination pattern of OCPs was in the order of HCHs > Drins > CHLs > DDTs congruent to HCB and HPTs. Estimated daily intakes (EDIs) through dietary consumption of cattle tissues were lower than the recommended acceptable daily intakes (ADIs) established by FAO/WHO. However, the hazard ratios (HRs) based on cancer risk were greater than 1.0 for HCHs based on the average and 95th centile concentrations, indicating carcinogenic effects to consumers through cattle tissues consumption. (C) 2015 Elsevier Ltd. All rights reserved.
  • Darwish WS, Ikenaka Y, Morshdy AE, Eldesoky KI, Nakayama S, Mizukawa H, Ishizuka M
    The Journal of veterinary medical science 78 2 351 - 354 2016年02月 [査読有り]
     研究論文(学術雑誌) 
    The aim of this study was to estimate total carotenoids, beta-carotene and retinol concentrations in the livers and muscles of some ungulates (cattle, buffalo, sheep, goats and horses) in comparison with the Wistar rats as a control. Cattle and horses had the highest contents of total carotenoids and beta-carotene. Unexpectedly, sheep was the highest accumulator of retinol with a mean concentration of 203 +/- 23.34 mu g/g, while the least accumulator was buffalo, having a mean value of 58.28 +/- 13.77 mu g/g. Livers had higher contents of the examined phytochemicals than muscles. Consumption of these tissues may provide human with some needs from these important phytochemicals, though ingestion of livers, especially that of the sheep, is not advisable for the pregnant women.
  • Hokuto Nakata, Shouta M. M. Nakayama, John Yabe, Allan Liazambi, Hazuki Mizukawa, Wageh Sobhy Darwish, Yoshinori Ikenaka, Mayumi Ishizuka
    ENVIRONMENTAL POLLUTION 208 Pt B 395 - 403 2016年01月 [査読有り]
     研究論文(学術雑誌) 
    Stable Pb isotope ratios (Pb-IRs) have been recognized as an efficient tool for identifying sources. This study carried out at Kabwe mining area, Zambia, to elucidate the presence or absence of Pb isotope fractionation in goat and chicken, to evaluate the reliability of identifying Pb pollution sources via analysis of Pb-IRs, and to assess whether a threshold for blood Pb levels (Pb-B) for biological fractionation was present The variation of Pb-IRs in goat decreased with an increase in Pb-B and were fixed at certain values close to those of the dominant source of Pb exposure at Pb-B > 5 mu g/dL. However, chickens did not show a clear relationship for Pb-IRs against Pb-B, or a fractionation threshold. Given these, the biological fractionation of Pb isotopes should not occur in chickens but in goats, and the threshold for triggering biological fractionation is at around 5 mu g/dL of Pb-B in goats. (C) 2015 Elsevier Ltd. All rights reserved.
  • Darwish WS, Ikenaka Y, Nakayama SM, Mizukawa H, Ishizuka M
    Journal of food science 81 1 T275 - T281 2016年01月 [査読有り]
     研究論文(学術雑誌) 
    Lead (Pb) is an environmental pollutant that can get entry into human body through contaminated foods, drinks, and inhaled air leading to severe biological consequences, and has been responsible for many deaths worldwide. The objectives of this study were 1st to investigate the modulatory effects of environmentally relevant concentrations of Pb on AhR gene battery, which is controlling xenobiotics metabolism. 2nd, trials to reduce Pb-induced adverse effects were done using some phytochemicals like beta-carotene or ascorbic acid. Human hepatoma (HepG2) cell lines were exposed to a wide range of Pb concentrations varying from physiological to toxic levels (0 to 10 mg/L) for 24 h. High Pb concentrations (1 to 10 mg/L) significantly reduced phase I (CYP1A1 and 1A2) and phase II (UGT1A6 and NQO1) xenobiotic metabolizing enzyme mRNA expression in a mechanistic manner through the AhR regulation pathway. Additionally, these Pb concentrations induced oxidative stress in HepG2 cells in terms of production of reactive oxygen species (ROS) and induced heme oxygenase-1 mRNA expression in a concentration-dependent phenomenon. Coexposure of HepG2 cells to physiological concentrations of some micronutrients, like beta-carotene (10 mu M) or ascorbic acid (0.1 mM), along with Pb (1 mg/L) for 24 h significantly reduced the levels of ROS production and recovered AhR mRNA expression into the normal levels. Thus, consumption of foods rich in these micronutrients may help to reduce the adverse effects of lead in areas with high levels of pollution.
  • Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Hisato Iwata, Jean Yoo, Akira Kubota, Miyuki Yamamoto, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Tatsuya Kunisue, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 50 1 444 - 452 2016年01月 [査読有り]
     研究論文(学術雑誌) 
    There are growing concerns about the increase in hyperthyroidism in pet cats due to exposure to organo-halogen contaminants and their hydroxylated metabolites. This study investigated the blood contaminants polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) and their hydroxylated and methoxylated derivatives (OH-PCBs, OH-PBDEs, and MeO-PBDEs), in pet dogs and cats. We also measured the residue levels of these compounds in commercially available pet foods. Chemical analyses of PCBs and OH-PCBs showed that the OH-PCB levels were 1 to 2 orders of magnitude lower in cat and dog food products than in their blood, suggesting that the origin of OH-PCBs in pet dogs and cats is PCBs ingested with their food. The major congeners of OH-/MeO-PBDEs identified in both pet food products and blood were natural products (60H-/MeO-BDE47 and 2'OH-/MeO-BDE68) from marine organisms. In particular, higher concentrations of 60H-BDE47 than 2'OH-BDE68 and two MeO-PBDE congeners were observed in the cat blood, although MeO-BDEs were dominant in cat foods, suggesting the efficient biotransformation of 60H-BDE47 from 6MeO-BDE47 in cats. We performed in vitro demethylation experiments to confirm the biotransformation of MeO-PBDEs to OH-PBDEs using liver microsomes. The results showed that 6MeO-BDE47 and 2'MeO-BDE68 were demethylated to 60H-BDE47 and 2'OH-BDE68 in both animals, whereas no hydroxylated metabolite from BDE47 was detected. The present study suggests that pet cats are exposed to MeO-PBDEs through cat food products containing fish flavors and that the OH-PBDEs in cat blood are derived from the CYP-dependent demethylation of naturally occurring MeO-PBDE congeners, not from the hydroxylation of PBDEs.
  • Jean Yoo, Masashi Hirano, Hazuki Mizukawa, Kei Nomiyama, Tetsuro Agusa, Eun-Young Kim, Shinsuke Tanabe, Hisato Iwata
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 49 24 14588 - 14596 2015年12月 [査読有り]
     研究論文(学術雑誌) 
    The aim of this study was to understand the cytochrome P450 (CYP)-dependent metabolic pathway and potency of polychlorinated biphenyls (PCBs) in the Baikal seal (Pusa sibirica). In vitro metabolism of 62 PCB congener mixtures was investigated by using liver microsomes of this species. A decreased ratio of over 20% was observed for CB3, CB4, CB8, CB15, CB19, CB22, CB37, CB54, CB77, and CB105, suggesting the preferential metabolism of low-chlorinated PCBs by CYPs. The highly activated metabolic pathways in Baikal seals that were predicted from the decreased PCBs and detected hydroxylated PCBs (OH-PCBs) were CB22 to 4'OH-CB20 and CB77 to 4'OH-CB79. The total amount of OH-PCBs detected as identified and unidentified congeners accounted for only a 3.8 +/- 1.7 mol % of loaded PCBs, indicating many unknown PCB metabolic pathways. To explore factors involved in CYP-dependent PCB metabolism, we examined the relationships among the structural and physicochemical properties of PCBs, the in silica PCB-CYP docking parameters, and the in vitro PCB decreased ratios by principal component analysis. Statistical analysis showed that the decreased PCB ratio was at least partly accounted for by the substituted chlorine number of PCBs and the distance from the CI-unsubstituted carbon of docked PCBs to the heme Fe in CYP2A and 2B.
  • Jemima Tiwaa Mario, Kazutoshi Fujioka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Yoshiko Aoyama, Mayumi Ishizuka, Kumiko Taira
    PLOS ONE 10 11 e0142172  2015年11月 [査読有り]
     研究論文(学術雑誌) 
    Neonicotinoid insecticides are nicotinic acetylcholine receptor agonists used worldwide. Their environmental health effects including neurotoxicity are of concern. We previously determined a metabolite of acetamiprid, N-desmethyl-acetamiprid in the urine of a patient, who exhibited some typical symptoms including neurological findings. We sought to investigate the association between urinary N-desmethyl-acetamiprid and the symptoms by a prevalence case-control study. Spot urine samples were collected from 35 symptomatic patients of unknown origin and 50 non-symptomatic volunteers (non-symptomatic group, NSG, 4-87 year-old). Patients with recent memory loss, finger tremor, and more than five of six symptoms (headache, general fatigue, palpitation/chest pain, abdominal pain, muscle pain/weakness/spasm, and cough) were in the typical symptomatic group (TSG, n = 19, 5-69 year-old); the rest were in the atypical symptomatic group (ASG, n = 16, 5-78 year-old). N-desmethyl-acetamiprid and six neonicotinoids in the urine were quantified by liquid chromatography-tandem mass spectrometry. The detection of N-desmethyl-acetamiprid was the most frequent and highest in TSG (47.4%, 6.0 ppb (frequency, maximum)), followed by in ASG (12.5%, 4.4 ppb) and in NSG (6.0%, 2.2 ppb), however acetamiprid was not detected. Thiamethoxam was detected in TSG (31.6%, 1.4 ppb), in ASG (6.3%, 1.9 ppb), but not in NSG. Nitenpyram was detected in TSG (10.5%, 1.2 ppb), in ASG (6.3%, not quantified) and in NSG (2.0%, not quantified). Clothianidin was only detected in ASG (6.3%, not quantified), and in NSG (2.0%, 1.6 ppb). Thiacloprid was detected in ASG (6.3%, 0.1 ppb). The cases in TSG with detection of N-desmethyl-acetamiprid and thiamethoxam were aged 5 to 62 years and 13 to 62 years, respectively. Detection of N-desmethyl-acetamiprid was associated with increased prevalence of the symptoms (odds ratio: 14, 95% confidence interval: 3.5-57). Urinary N-desmethyl-acetamiprid can be used as a biomarker for environmental exposure to acetamiprid. Further multi-centered clinical research in larger patients groups with more metabolites analysis is needed.
  • Wageh Sobhy Darwish, Yoshinori Ikenaka, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    JAPANESE JOURNAL OF VETERINARY RESEARCH 63 4 173 - 182 2015年11月 [査読有り]
     研究論文(学術雑誌) 
    Cooking of meat usually aims in producing microbiologically safe food suitable for human consumption. However, doing so at such high temperatures may produce some cooking toxicants or mutagens. The objectives of this study were to investigate the mutagenicity of modelled-heat-treated meat after different cooking methods (boiling, pan-frying and charcoal grilling) using Ames Salmonella typhimurium mutagenicity assay. In addition, the content of benzo[a]pyrene (B[a]P) in the meat extracts prepared under different cooking methods were measured using HPLC. In a trial to investigate the causes behind the mutagenicity of different meat extracts, HepG2 cell line was exposed to different modelled-heat-treated meat extracts. mRNA expression levels of various phase I and II xenobiotic metabolizing enzymes (XMEs) were examined using real time PCR. The results obtained declared that pan-fried and charcoal grilled-meat extracts significantly induced production of histidine+ revertants in the Ames mutagenicity assay. Grilled-meat extracts had the highest residual concentrations of B[a]P followed by pan-fried-meat, boiled meat and raw meat extracts, respectively. Induction of XMEs especially CYP1A1, CYP1A2 and NQO1 may contribute to the mutagenic ability of these extracts. It is highly advisable to control cooking temperature, time and method in order to reduce cooked-meat mutagens.
  • Kensuke P. Watanabe, Minami Kawata, Yoshinori Ikenaka, Shouta M. M. Nakayama, Chihiro Ishii, Wageh Sobhi Darwish, Aksorn Saengtienchai, Hazuki Mizukawa, Mayumi Ishizuka
    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 34 10 2328 - 2334 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    Coumarin-derivative anticoagulant rodenticides used for rodent control are posing a serious risk to wild bird populations. For warfarin, a classic coumarin derivative, chickens have a high median lethal dose (LD50), whereas mammalian species generally have much lower LD50. Large interspecies differences in sensitivity to warfarin are to be expected. The authors previously reported substantial differences in warfarin metabolism among avian species; however, the actual in vivo pharmacokinetics have yet to be elucidated, even in the chicken. In the present study, the authors sought to provide an in-depth characterization of warfarin metabolism in birds using in vivo and in vitro approaches. A kinetic analysis of warfarin metabolism was performed using liver microsomes of 4 avian species, and the metabolic abilities of the chicken and crow were much higher in comparison with those of the mallard and ostrich. Analysis of in vivo metabolites from chickens showed that excretions predominantly consisted of 4-hydroxywarfarin, which was consistent with the in vitro results. Pharmacokinetic analysis suggested that chickens have an unexpectedly long half-life despite showing high metabolic ability in vitro. The results suggest that the half-life of warfarin in other bird species could be longer than that in the chicken and that warfarin metabolism may not be a critical determinant of species differences with respect to warfarin sensitivity. Environ Toxicol Chem 2015;34:2328-2334. (c) 2015 SETAC
  • Hokuto Nakata, Shouta M. M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Chihiro Ishii, Yared B. Yohannes, Satoru Konnai, Wageh Sobhy Darwish, Mayumi Ishizuka
    ENVIRONMENTAL POLLUTION 205 8 - 15 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    Nairobi city in Kenya produces 2000 tons/day of garbage, and most of it is dumped onto the Dandora dumping site, home to a quarter-million residents. This study was conducted (1) to assess the contamination levels of nine metals and a metalloid (arsenic) in the blood of pigs, goats, sheep and cattle from Dandora, and (2) to identify a possible source of lead (Pb) pollution. Cadmium (Cd, 0.17-435 mu g/kg, dry-wt) and Pb (90-2710 mu g/kg) levels in blood were generally high, suggesting human exposure to Cd through livestock consumption and Pb poisoning among pigs (2600 mu g/kg) and cattle (354 mu g/kg). Results of Pb isotope ratios indicated that the major exposure route might differ among species. Our results also suggested a possibility that the residents in Dandora have been exposed to the metals through livestock consumption. (C) 2015 Elsevier Ltd. All rights reserved.
  • Aksorn Saengtienchai, Yoshinori Ikenaka, Wageh Sobhy Darwish, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    JOURNAL OF VETERINARY MEDICAL SCIENCE 77 10 1261 - 1267 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    Pyrene (PY) is a polycyclic aromatic hydrocarbon (PAH) that is often used as a biomarker for human and wildlife exposure to PAHs. As the metabolites of PAHs, similar to their parent compounds, pose public health risks, it is necessary to study their characteristics and tissue-specific distribution. The present study was performed to experimentally characterize PY metabolites and analyze the tissue-specific distribution of the conjugated metabolites after oral administration of PY to rats. PY metabolites, such as pyrenediol-disulfate (PYdiol-diS), pyrenediol-sulfate (PYdiol-S), pyrene-1-sufate (PYOS), pyrene-l-glucuronide (PYOG) and 1-hydroxypyrene (PYOH), were detected in rat urine. Although glucuronide conjugate was the predominant metabolite, the metabolite composition varied among tissues. Interestingly, the proportion of PYOH was high in the large intestine. Furthermore, PYOH was the only PY metabolite detected in feces.
  • Mayu Kakehi, Yoshinori Ikenaka, Shouta M. M. Nakayama, Yusuke K. Kawai, Kensuke P. Watanabe, Hazuki Mizukawa, Kei Nomiyama, Shinsuke Tanabe, Mayumi Ishizuka
    TOXICOLOGICAL SCIENCES 147 2 360 - 369 2015年10月 [査読有り]
     研究論文(学術雑誌) 
    There are various interspecies differences in xenobiotic-metabolizing enzymes. It is known that cats show slow glucuronidation of drugs such as acetaminophen and strong side effects due to the UGT1A6 pseudogene. Recently, the UGT1A6 pseudogene was found in the Northern elephant seal and Otariidae was suggested to be UGT1A6-deficient. From the results of measurements of uridine diphosphate-glucuronosyltransferase (UGT) activity using liver microsomes, the Steller sea lion, Northern fur seal, and Caspian seal showed UGT activity toward 1-hydroxypyrene and acetaminophen as low as in cats, which was significantly lower than in rat and dog. Furthermore, UGT1A6 pseudogenes were found in Steller sea lion and Northern fur seal, and all Otariidae species were suggested to have the UGT1A6 pseudogene. The UGT1 family genes appear to have undergone birth-and-death evolution based on a phylogenetic and synteny analysis of the UGT1 family in mammals including Carnivora. UGT1A2-1A5 and UGT1A7-1A10 are paralogous genes to UGT1A1 and UGTA6, respectively, and their numbers were lower in cat, ferret and Pacific walrus than in human, rat, and dog. Felidae and Pinnipedia, which are less exposed to natural xenobiotics such as plant-derived toxins due to their carnivorous diet, have experienced fewer gene duplications of xenobiotic-metabolizing UGT genes, and even possess UGT1A6 pseudogenes. Artificial environmental pollutants and drugs conjugated by UGT are increasing dramatically, and their elimination to the environment can be of great consequence to cat and Pinnipedia species, whose low xenobiotic glucuronidation capacity makes them highly sensitive to these compounds.
  • Nesta Bortey-Sam, Shouta M M Nakayama, Osei Akoto, Yoshinori Ikenaka, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    International journal of environmental research and public health 12 9 11448 - 65 2015年09月 [査読有り]
     
    Heavy metals and a metalloid in agricultural soils in 19 communities in Tarkwa were analyzed to assess the potential ecological risk. A total of 147 soil samples were collected in June, 2012 and analyzed for As, Cd, Co, Cr, Cu, Hg, Ni, Pb and Zn. Mean concentrations (mg/kg dw) of heavy metals in the communities decreased in order of Zn (39) ˃ Cr (21) ˃ Pb (7.2) ˃ Cu (6.2) ˃ As (4.4) ˃ Ni (3.7) ˃ Co (1.8) ˃ Hg (0.32) ˃ Cd (0.050). Correlations among heavy metals and soil properties indicated that soil organic matter could have substantial influence on the total contents of these metals in soil. From the results, integrated pollution (C(deg)) in some communities such as, Wangarakrom (11), Badukrom (13) and T-Tamso (17) indicated high pollution with toxic metals, especially from As and Hg. Potential ecological risk (RI) indices indicated low (Mile 7) to high risks (Wangarakrom; Badukrom) of metals. Based on pollution coefficient (C(i)(f)), C(deg), monomial ecological risk (E(i)(r)) and RI, the investigated soils fall within low to high contamination and risk of heavy metals to the ecological system especially plants, soil invertebrates and/or mammalian wildlife. This represented moderate potential ecological risk in the study area, and mining activities have played a significant role.
  • Nesta Bortey-Sam, Shouta M. M. Nakayama, Osei Akoto, Yoshinori Ikenaka, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 12 9 11448 - 11465 2015年09月 [査読有り]
     研究論文(学術雑誌) 
    Heavy metals and a metalloid in agricultural soils in 19 communities in Tarkwa were analyzed to assess the potential ecological risk. A total of 147 soil samples were collected in June, 2012 and analyzed for As, Cd, Co, Cr, Cu, Hg, Ni, Pb and Zn. Mean concentrations (mg/kg dw) of heavy metals in the communities decreased in order of Zn (39) ? Cr (21) ? Pb (7.2) ? Cu (6.2) ? As (4.4) ? Ni (3.7) ? Co (1.8) ? Hg (0.32) ? Cd (0.050). Correlations among heavy metals and soil properties indicated that soil organic matter could have substantial influence on the total contents of these metals in soil. From the results, integrated pollution (C-deg) in some communities such as, Wangarakrom (11), Badukrom (13) and T-Tamso (17) indicated high pollution with toxic metals, especially from As and Hg. Potential ecological risk (RI) indices indicated low (Mile 7) to high risks (Wangarakrom; Badukrom) of metals. Based on pollution coefficient (C-f(i)), C-deg, monomial ecological risk (E-r(i)) and RI, the investigated soils fall within low to high contamination and risk of heavy metals to the ecological system especially plants, soil invertebrates and/or mammalian wildlife. This represented moderate potential ecological risk in the study area, and mining activities have played a significant role.
  • Nesta Bortey-Sam, Shouta M. M. Nakayama, Osei Akoto, Yoshinori Ikenaka, Julius N. Fobil, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 12 8 8811 - 8827 2015年08月 [査読有り]
     研究論文(学術雑誌) 
    This study was carried out to assess the extent of heavy metals and metalloid accumulation from agricultural soils to foodstuffs (viz, M. esculenta (cassava) and Musa paradisiaca (plantain)) around thirteen neighboring communities within Tarkwa, Ghana; and to estimate the human health risk associated with consumption of these foodstuffs. Concentrations of As, Cd, Co, Cr, Cu, Ni, Pb, and Zn were measured with an inductively coupled plasma-mass spectrometer and mercury analysis was done using a mercury analyzer. From the results, 30% of cassava samples collected, contained higher concentrations of Pb when compared to Codex Alimentarius Commission standard values. Bioconcentration factor indicated that Ni had higher capacity of absorption into food crops from soil than the other heavy metals. For both children and adults, the target hazard quotient (THQ) of Pb in cassava in communities such as Techiman, Wangarakrom, Samahu, and Tebe (only children) were greater than 1, which is defined as an acceptable risk value. This indicated that residents could be exposed to significant health risks associated with cassava consumption.
  • Nesta Bortey-Sam, Shouta M M Nakayama, Osei Akoto, Yoshinori Ikenaka, Julius N Fobil, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    International journal of environmental research and public health 12 8 8811 - 27 2015年07月 [査読有り]
     
    This study was carried out to assess the extent of heavy metals and metalloid accumulation from agricultural soils to foodstuffs (viz, M. esculenta (cassava) and Musa paradisiaca (plantain)) around thirteen neighboring communities within Tarkwa, Ghana; and to estimate the human health risk associated with consumption of these foodstuffs. Concentrations of As, Cd, Co, Cr, Cu, Ni, Pb, and Zn were measured with an inductively coupled plasma-mass spectrometer and mercury analysis was done using a mercury analyzer. From the results, 30% of cassava samples collected, contained higher concentrations of Pb when compared to Codex Alimentarius Commission standard values. Bioconcentration factor indicated that Ni had higher capacity of absorption into food crops from soil than the other heavy metals. For both children and adults, the target hazard quotient (THQ) of Pb in cassava in communities such as Techiman, Wangarakrom, Samahu, and Tebe (only children) were greater than 1, which is defined as an acceptable risk value. This indicated that residents could be exposed to significant health risks associated with cassava consumption.
  • Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M. M. Nakayama, Yared Beyene Yohannes, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 22 13 9658 - 9667 2015年07月 [査読有り]
     研究論文(学術雑誌) 
    Airborne particulate samples were collected on quartz filters to determine the concentrations, sources and health risks of polycyclic aromatic hydrocarbons (PAHs) in air in Kumasi, Ghana. A total of 32 air samples were collected in Kwame Nkrumah University of Science and Technology (KNUST) campus (pristine site) and city centre (CC). Samples were extracted with 1:2v/v acetone/hexane mixture prior to GC-MS analyses. The sum of concentrations of 17 PAHs in air ranged from 0.51 to 16 (KNUST) and 19-38 ng/m(3) (CC). The concentration of benzo[a]pyrene, BaP, ranged from below detection limit to 0.08 ng/m(3) (KNUST) and 1.6 to 5.6 ng/m(3) (CC). Chemical mass balance model showed that PAHs in air in Kumasi were mainly from fuel combustion. The total BaP equivalent concentration (BaPeq) in CC was 18 times higher compared to KNUST; based on the European Legislation and Swedish and UK Standards for BaP in air, CC could be classified as highly polluted. Estimated carcinogenicity of PAHs in terms of BaPeq indicated that BaP was the principal PAH contributor in CC (70 %). Health risk to adults and children associated with PAH inhalation was assessed by taking into account the lifetime average daily dose and corresponding incremental lifetime cancer risk (ILCR). The ILCR was within the acceptable range (10(-6) to 10(-4)), indicating low health risk to residents.
  • Nesta Bortey-Sam, Shouta M. M. Nakayama, Yoshinori Ikenaka, Osei Akoto, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    ENVIRONMENTAL MONITORING AND ASSESSMENT 187 7 397  2015年07月 [査読有り]
     研究論文(学術雑誌) 
    Concentrations of heavy metals and metalloid in borehole drinking water from 18 communities in Tarkwa, Ghana, were measured to assess the health risk associated with its consumption. Mean concentrations of heavy metals (mu g/L) exceeded recommended values in some communities. If we take into consideration the additive effect of heavy metals and metalloid, then oral hazard index (HI) results raise concerns about the noncarcinogenic adverse health effects of drinking groundwater in Huniso. According to the US Environmental Protection Agency's (USEPA) guidelines, HI values indicating noncarcinogenic health risk for adults and children in Huniso were 0.781 (low risk) and 1.08 (medium risk), respectively. The cancer risk due to cadmium (Cd) exposure in adults and children in the sampled communities was very low. However, the average risk values of arsenic (As) for adults and children through drinking borehole water in the communities indicated medium cancer risk, but high cancer risk in some communities such as Samahu and Mile 7. Based on the USEPA assessment, the average cancer risk values of As for adults (3.65E-05) and children (5.08E-05) indicated three (adults) and five (children) cases of neoplasm in a hundred thousand inhabitants. The results of this study showed that residents in Tarkwa who use and drink water from boreholes could be at serious risk from exposure to these heavy metals and metalloid.
  • Yoshinori Ikenaka, Shouta M. M. Nakayama, Mami Oguri, Aksorn Saengtienchai, Hazuki Mizukawa, Jun Kobayashi, Wageh Sobhy Darwish, Mayumi Ishizuka
    ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 39 3 1148 - 1153 2015年05月 [査読有り]
     研究論文(学術雑誌) 
    Red gourami (Colisa labiosa) have previously been shown to have low levels of pyrene-metabolizing activity. In this study, other pharmacokinetic factors of pyrene in C. labiosa were compared to those in Japanese medaka (Oryzias latipes). Results indicated that the two species labiosa absorbed pyrene in similar amounts. However, excretion of pyrene metabolites from C. labiosa over an 8-day period was lower than those from O. latipes. These findings show that C. labiosa has low ability to metabolize pyrene and to excrete pyrene and its metabolites from the body, and is therefore considered an accumulator of these chemicals. C. labiosa has unique characteristics with regard to pyrene pharmacokinetics. Knowledge about interspecies differences in pharmacokinetics is crucial in determining the endangered species to xenobiotic exposure. (C) 2015 Elsevier B.V. All rights reserved.
  • K. P. Watanabe, Y. K. Kawai, S. M. M. Nakayama, Y. Ikenaka, H. Mizukawa, N. Takaesu, M. Ito, S. -I. Ikushiro, T. Sakaki, M. Ishizuka
    JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 38 2 190 - 195 2015年04月 [査読有り]
     研究論文(学術雑誌) 
    Large interspecies differences in avian xenobiotic metabolism have been revealed by microsome-based studies, but specific enzyme isoforms in different bird species have not yet been compared. We have previously shown that CYP2C23 genes are the most induced CYP isoforms in chicken liver. In this study, we collected partial CYP2C23a gene sequences from eight avian species (ostrich, blue-eared pheasant, snowy owl, great-horned owl, Chilean flamingo, peregrin falcon, Humboldt penguin, and black-crowned night heron) selected to cover the whole avian lineage: Paleognathae, Galloanserae, and Neoaves. Genetic analysis showed that CYP2C23 genes of Galloanserae species (chicken and blue-eared pheasant) had unique characteristics. We found some duplicated genes (CYP2C23a and CYP2C23b) and two missing amino acid residues in Galloanserae compared to the other two lineages. The genes have lower homology than in other avian lineages, which suggests Galloanserae-specific rapid evolutionary changes. These genetic features suggested that the Galloanserae are not the most representative avian species, considering that the Neoaves comprise more than 95% of birds. Moreover, we succeeded in synthesizing an antipeptide polyclonal antibody against the region of CYP2C23 protein conserved in avians. However, comparative quantitation of CYP2C23 proteins in livers from six species showed that expression levels of these proteins differed no more than fourfold. Further study is needed to clarify the function of avian CYP2C23 proteins.
  • Hazuki Mizukawa, Kei Nomiyama, Tatsuya Kunisue, Michio X. Watanabe, Annamalai Subramanian, Hisato Iwata, Mayumi Ishizuka, Shinsuke Tanabe
    ENVIRONMENTAL RESEARCH 138 255 - 263 2015年04月 [査読有り]
     研究論文(学術雑誌) 
    The concentrations of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and their hydroxylated metabolites (OH-PCBs and OH-PBDEs) were measured in the blood of Eurasian wild pigs (Sus scrofa) from a municipal waste open dumping site (DS) and a reference site (RS) in South India. We showed that contamination with OH-PCBs was higher in female pigs from the DS than in all other adult pigs. The highest OH-PCB concentrations were found in piglets from the DS. Moreover, the hepatic expression levels of CYP1A and CYP2B were higher in piglets than in their dam, implying metabolism of PCBs by cytochrome P450 (CYP) enzymes. The OH-PCB congener profiles differed according to sex and collection sites, possibly because of variations in the expression levels of phase I and phase II enzymes among individual pigs, differences in the exposure sources, and maternal transfer of parent PCBs. The hepatic CYP1A expression levels were positively correlated with the blood concentrations of 4OH-CB107, 4OH-CB162, and 4OH-CB187, implying CYP1A-dependent formation of these OH-PCBs in the pig liver. We found no significant correlations between the blood concentrations of OH-PCBs and thyroid hormones (THs); however, the thyroxin (T-4) levels were lower in pigs from the DS than in pigs from the RS. Our limited dataset suggest that induced CYP enzymes accelerate the metabolism of xenobiotics and endogenous molecules in pigs. Thus, besides parental compounds, the risk of hydroxylated metabolites entering wildlife and humans living in and around municipal open waste dumping sites should be considered. (C) 2015 Elsevier Inc. All rights reserved.
  • Nesta Bortey-Sam, Shouta M. M. Nakayama, Yoshinori Ikenaka, Osei Akoto, Elvis Baidoo, Yared Beyene Yohannes, Hazuki Mizukawa, Mayumi Ishizuka
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 111 160 - 167 2015年01月 [査読有り]
     研究論文(学術雑誌) 
    Heavy metal and metalloid contamination in food resulting from mining is of major concern due to the potential risk involved. Food consumption is the most likely route of human exposure to metals. This study was therefore to assess metals in different organs and different animal species near gold mines used for human consumption (free-range chicken, goat and sheep) in Tarkwa, Ghana, and to estimate the daily intake and health risk. The concentrations of Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb were measured with an inductively coupled plasma-mass spectrometer and Hg analysis was done using the mercury analyzer. Principal component analysis of the results showed a clear separation between chicken, grouped on one side, and the ruminants clustered on another side in both offal and muscle. Interestingly, As, Cd, Hg, Mn and Pb made one cluster in the offal of chicken. Chicken muscle also showed similar distribution with As, Hg and Pb clustered together. The daily intake of metals (mu g/kg body weight/day) were in the following ranges; As [0.002 (kidneys of goat and sheep)-0.19 (chicken gizzard)], Cd [0.003 (chicken muscle)-0.55 (chicken liver)], Hg [0.002 (goat muscle)-0.29 (chicken liver)], Pb [0.01 (muscles and kidneys of goat and sheep)-0.96 (chicken gizzard)] and Mn [0.13 (goat kidney)-8.92 (sheep liver)]. From the results, daily intakes of As, Cd, Hg, Pb and Mn in these food animals were low compared to the provisional tolerable daily intake guidelines. The THQs although less than one, indicated that contributions of chicken gizzard and liver to toxic metal exposure in adults and especially children could be significant. (C) 2014 Published by Elsevier Inc.
  • Nesta Bortey-Sam, Yoshinori Ikenaka, Shouta M. M. Nakayama, Osei Akoto, Yared Beyene Yohannes, Elvis Baidoo, Hazuki Mizukawa, Mayumi Ishizuka
    SCIENCE OF THE TOTAL ENVIRONMENT 496 471 - 478 2014年10月 [査読有り]
     研究論文(学術雑誌) 
    As a developing country, the economic and population growth rates in Ghana over the past few years have seen a tremendous increase. The growing rate of industrialization is gradually leading to contamination and deterioration of the environment and pollution is likely to reach disturbing levels. Surface soil samples were collected randomly from 36 communities in the Kumasi Metropolis, Ghana, to determine the concentrations, distribution, sources and toxic potential of emission of polycyclic aromatic hydrocarbons (PAHs) since no such comprehensive study had been conducted. The mean concentration of total PAHs in the surface soils in each community ranged from 14.78 at Ahinsan to 2084 ng/g dry weight at Adum with an average of 442.5 +/- 527.2 ng/g dry weight. Diagnostic ratios and chemical mass balance models of the results showed that PAHs in surface soil samples from the study area were mainly from fuel combustion. Carcinogenic potency of PAH load from the city centre was approximately 150 times higher as compared to a pristine site, Kwame Nkrumah University of Science and Technology's Botanical Garden. BaP, a human carcinogen, contributed 70% of the total PAHs toxicity level from the city centre of Kumasi. (C) 2014 Elsevier B.V. All rights reserved.
  • Aksorn Saengtienchai, Yoshinori Ikenaka, Shouta M. M. Nakayama, Hazuki Mizukawa, Mayu Kakehi, Nesta Bortey-Sam, Wageh Sobhy Darwish, Toshio Tsubota, Masanori Terasaki, Amnart Poapolathep, Mayumi Ishizuka
    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 33 9 2062 - 2069 2014年09月 [査読有り]
     研究論文(学術雑誌) 
    Interspecific differences in xenobiotic metabolism are a key to determining relative sensitivities of animals to xenobiotics. However, information on domesticated livestock, companion animals, and captive and free-ranging wildlife is incomplete. The present study evaluated interspecific differences in phase II conjugation using pyrene as a nondestructive biomarker of polycyclic aromatic hydrocarbon (PAH) exposure. Polycyclic aromatic hydrocarbons and their metabolites have carcinogenic and endocrine-disrupting effects in humans and wildlife and can have serious consequences. The authors collected urine from 16 mammalian species and analyzed pyrene metabolites. Interspecific differences in urinary pyrene metabolites, especially in the concentration and composition of phase II conjugated metabolites, were apparent. Glucuronide conjugates are dominant metabolites in the urine of many species, including deer, cattle, pigs, horses, and humans. However, they could not be detected in ferret urine even though the gene for ferret Uridine 5'-diphospho-glucuronosyltransferase (UDP-glucuronosyltransferase, UGT) 1A6 is not a pseudogene. Sulfate conjugates were detected mainly in the urine of cats, ferrets, and rabbits. Interestingly, sulfate conjugates were detected in pig urine. Although pigs are known to have limited aryl sulfotransferase activity, the present study demonstrated that pig liver was active in 1-hydroxypyrene sulfation. The findings have some application for biomonitoring environmental pollution. (c) 2014 SETAC
  • Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M. M. Nakayama, Kentaro Q. Sakamoto, Shoichi Fujita, Mayumi Ishizuka
    JAPANESE JOURNAL OF VETERINARY RESEARCH 62 3 107 - 115 2014年08月 [査読有り]
     研究論文(学術雑誌) 
    Contamination levels of coplanar polychlorinated biphenyls (Co-PCBs), polycyclic aromatic hydrocarbons (PAHs), and dichlorodiphenyltrichloroethane (DDTs) were measured in the entire body of the large Japanese field mouse (Apodemus speciosus) collected from Hokkaido (Ishikari and Rankoshi) and Aomori prefecture (Takko) in Japan. Higher concentrations of PCBs including Co-PCBs, were observed in the mice collected from Ishikari than those from Rankoshi. The concentration of PAHs in the soil from Ishikari was also higher than that in the other sampling sites. The findings suggest that Ishikari is the most polluted area, probably because of human activities, depending on the population distribution. However, the observed contaminant levels were extremely lower compared to those in previous studies. The ratio of testis weight to body weight (TW/BW) was the lowest in the mice collected from Ishikari, which is the area contaminated with PAHs and p,p'-dichlorodiphenyldichloroethylene (DDE). However, the serum testosterone levels of mice from the Ishikari area were higher than those from the non-contaminated other areas although no significant differences. Previous studies have shown that a low-level exposure to dioxin related compounds (DRCs) disturbances in sexual function, resulting in the production of testosterone. This study showed that POPs exposure is one of the possibility of the high testosterone concentration in the mice of the Ishikari area in addition to a cause of biological and environmental factors such as habitat density, age, temperatures and/or food riches.
  • Chihiro Ishii, Yoshinori Ikenaka, Shouta M. M. Nakayama, Yuya Suzuki, Yutaka Watanuki, Yuji Watanabe, Yared Beyene Yohannes, Hazuki Mizukawa, Mayumi Ishizuka
    JAPANESE JOURNAL OF VETERINARY RESEARCH 62 3 143 - 149 2014年08月 [査読有り]
     研究論文(学術雑誌) 
    Teuri Island, Hokkaido in Japan is an important place for seabirds breeding. We measured the concentrations of heavy metals (Hg, Cd, Cr, Co, Ni, Cu, Zn, and Pb) and a metalloid (As) in rhinoceros auklet (Cerorhinca monocerata) (n = 7), thick-billed murre (Uria lomvia) (n = 2), spectacled guillemot (Cepphus carbo) (n = 6), slaty-backed gull (Larus schistisagus) (n = 15), jungle crow (Corvus macrorhynchos) (n = 3), Japanese anchovy (Engraulis japonica) (n = 6) and Atka mackerel (Pleurogrammus azonus) (n = 2). Spectacled guillemot had high As concentrations, with its source being their feeding habitat. Concentration of Hg in kidney of jungle crow was higher than other seabird species at Teuri.
  • Kei Nomiyama, Chika Kanbara, Mari Ochiai, Akifumi Eguchi, Hazuki Mizukawa, Tomohiko Isobe, Takashi Matsuishi, Tadasu K. Yamada, Shinsuke Tanabe
    MARINE ENVIRONMENTAL RESEARCH 93 15 - 22 2014年02月 [査読有り]
     研究論文(学術雑誌) 
    Information on accumulation of halogenated phenolic contaminants in the blood of marine mammal is limited. The present study, we determined the residue levels and patterns of chlorinated and brominated phenolic contaminants (OH-PCBs, OH-PBDEs and bromophenols) in the blood collected from pinnipeds (northern fur seal, spotted seal, Steller sea lion and ribbon seal) and small cetaceans (harbor porpoise and Dall's porpoise) from Japanese coastal waters. Concentrations of PCBs and OH-PCBs found in pinnipeds were the same as in small cetaceans living in the same coastal area. However, significantly lower concentrations of brominated compounds (PBDEs, MeO-PBDEs, OH-PBDEs) were found in the blood of pinnipeds than the levels found in cetacean species which live same area (p < 0.05). This difference of accumulation pattern suggested pinnipeds have an enhanced capability to degrade organobromine compounds relative to cetaceans. (C) 2013 Elsevier Ltd. All rights reserved.
  • Akifumi Eguchi, Kei Nomiyama, Mari Ochiai, Hazuki Mizukawa, Yasuko Nagano, Katsuhiro Nakagawa, Kouki Tanaka, Haruhiko Miyagawa, Shinsuke Tanabe
    TALANTA 118 253 - 261 2014年01月 [査読有り]
     研究論文(学術雑誌) 
    In this study, we developed a comprehensive, highly sensitive, and robust method for determining 53 congeners of three to eight chlorinated OH-PCBs in liver and brain samples by using isotope dilution gas chromatography (GC) coupled with electron capture negative ionization mass spectrometry (ECNI-MS). These results were compared with those from GC coupled with electron ionization high-resolution mass spectrometry (El-HRMS). Clean-up procedures for analysis of OH-PCBs homologs in liver and brain samples involve a pretreatment step consisting of acetonitrile partition and 5% hydrated silica-gel chromatography before derivatization. Recovery rates of tri- and tetra-chlorinated OH-PCBs in the acetonitrile partition method followed by the 5% hydrated silica-gel column (82% and 91%) were higher than conventional sulfuric acid treatment (2.0% and 3.5%). The method detection limits of OH-PCBs for each matrix obtained by GC/ECNI-MS and GC/EI-HRMS were 0.58-2.6 pg g(-1) and 0.36-1.6 pg g(-1) wet wt, respectively. Recovery rates of OH-PCB congeners in spike tests using sample matrices (10 and 50 pg) were 64.7-117% (CV: 4.7-14%) and 70.4-120% (CV: 2.3-12%), respectively. This analytical method may enable the simultaneous detection of various OH-PCBs from complex tissue matrices. Furthermore, this method allows more comprehensive assessment of the biological effects of OH-PCB exposure on critical organs. (C) 2013 Elsevier B.V. All rights reserved.
  • Mari Ochiai, Kei Nomiyama, Tomohiko Isobe, Hazuki Mizukawa, Tadasu K. Yamada, Yuko Tajima, Takashi Matsuishi, Masao Amano, Shinsuke Tanabe
    CHEMOSPHERE 92 7 803 - 810 2013年08月 [査読有り]
     研究論文(学術雑誌) 
    The present study investigated polychlorinated biphenyls (PCBs) and hydroxylated metabolites of PCBs (OH-PCBs) in blood from three porpoise species: finless porpoises (Neophocaena phocaenoides), harbor porpoises (Phocoena phocoena), and Dall's porpoises (Phocoenoides dalli). The porpoises were found stranded or were bycaught along the Japanese coast. Concentrations of OH-PCB were the highest in Dall's porpoises (58 pg g(-1) wet wt), second highest in finless porpoises (20 pg g(-1) wet wt), and lowest in harbor porpoises (8.3 pg g(-1) wet wt). The concentrations in Dall's porpoises were significantly higher than the concentrations in finless porpoises and harbor porpoises (p < 0.05 and p < 0.01, respectively). There was a positive correlation between PCB and OH-PCB concentrations (r = 0.67, p < 0.001), suggesting the possible concentration-dependent induction of CYP enzymes. The three porpoise species may have exceptionally low metabolic capacities compared with other marine and terrestrial mammals, because low OH-PCB/PCB concentration ratios were found, which were 0.0016 for Dall's porpoises, 0.0013 for harbor porpoises, and 0.00058 for finless porpoises. Distinct differences in the OH-PCB congener patterns were observed for the three species, even though they are taxonomically closely related. (c) 2013 Elsevier Ltd. All rights reserved.
  • Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Shuuji Yachimori, Terutake Hayashi, Yutaka Tashiro, Yasuko Nagano, Shinsuke Tanabe
    ENVIRONMENTAL POLLUTION 174 28 - 37 2013年03月 [査読有り]
     研究論文(学術雑誌) 
    Residue levels and patterns of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), their hydroxylated metabolites (OH-PCBs, OH-PBDEs), and methoxylated PBDEs (MeO-PBDEs) in the blood of various terrestrial mammals in Japan, including cats, raccoon dogs, dogs, masked palm civets, foxes, raccoons, badgers, and mongooses were determined. Tri- through penta-chlorinated OH-PCBs were predominant in cat blood, whereas hexa- through octa-chlorinated OH-PCBs were found in other species. High proportion of BDE209 was found in all species, suggesting exposure to municipal waste and soil containing higher levels of deca-BDE products. 6OH-/MeO-BDE47 and 2'OH-/MeO-BDE68 were dominant in all terrestrial mammals. This is first report on the detection of OH-/MeO-PBDEs in the blood of terrestrial mammals. High concentrations of OH-/MeO-PBDEs were found in cats, suggesting the intake of these compounds from seafood. Cats exhibited higher accumulation and specific patterns of OH-PCBs, OH-PBDEs, and MeO-PBDEs, they may be at a high risk from these metabolites. (C) 2012 Elsevier Ltd. All rights reserved.
  • Kei Nomiyama, Akifumi Eguchi, Hazuki Mizukawa, Mari Ochiai, Satoko Murata, Masayuki Someya, Tomohiko Isobe, Tadasu K. Yamada, Shinsuke Tanabe
    ENVIRONMENTAL POLLUTION 159 12 3364 - 3373 2011年12月 [査読有り]
     研究論文(学術雑誌) 
    We determined the residue levels and patterns of hydroxylated polybrominated diphenyl ethers (OH-PBDEs), and related compounds, such as PBDEs, methoxylated PBDEs (MeO-PBDEs), and bromophenols (BPhs) in the blood of eleven cetacean species stranded along the Japanese coasts. The dominant OH- and MeO-PBDE isomers found in all cetaceans were 6OH-BDE47 and 6MeO-BDE47. Additionally, 2,4,6-triBPh was dominant isomer in all cetaceans. In contrast, specific differences in the distribution of para- and meta- OH-PBDE isomers and some BPhs (potential PBDEs metabolites) were found among the cetaceans. Residue levels of Sigma MeO-PBDEs and 6OH-BDE47 + 2'OH-BDE68, and 2,4,6-triBPh and 6OH-BDE47 + 2'OH-BDE68 showed a significant positive correlation. These results may suggest that the large percentages of OH-PBDEs, MeO-PBDEs and 2,4,6-triBPh might share common source (i.e. biosynthesis by marine organisms), or metabolic pathway in cetacean species. Significant correlations were found between the concentrations of BDE99 and 2,4,5-triBPh. This result suggested that 2,4,5-triBPh in cetaceans could be a metabolite of BDE99. (C) 2011 Elsevier Ltd. All rights reserved.
  • Kei Nomiyama, Yukiko Uchiyama, Satoko Horiuchi, Akifumi Eguchi, Hazuki Mizukawa, Sawako Horai Hirata, Ryota Shinohara, Shinsuke Tanabe
    CHEMOSPHERE 85 3 315 - 321 2011年10月 [査読有り]
     研究論文(学術雑誌) 
    Information on accumulation of polychlorinated biphenyl metabolites (OH-PCBs) and hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in the blood of marine fish is limited. The present study, we determined the residue levels and patterns of PCBs, OH-PCBs, PBDEs, OH-PBDEs and methoxylated PBDEs (MeO-PBDEs) in the blood collected from scalloped hammerhead shark (Sphyrna lewini) and Japanese amberjack (Seriola quinqueradiata), species of predatory fish at Japanese coastal waters. The predominant homologues found in Japanese amberjacks were mono- and di-chlorinated OH-PCBs, and scalloped hammerhead sharks were octa-chlorinated OH-PCBs. The predominant OH-PCB isomers were lower-chlorinated OH-PCBs such as 6OH-CB2 and 2'OH-CB9 in Japanese amberjacks. This result suggests that exposure of Japanese amberjacks to lower-chlorinated OH-PCBs might be from the ambient aquatic environment. In scalloped hammerhead sharks, 4,4'diOH-CB202, 4OH-CB201 and 4OH-CB146 were the predominant isomers accounting for approximately 60% of the total OH-PCBs. The predominant MeO-PBDE isomers were 6MeO-BDE47 followed by 2'MeO-BDE68 in both species. As for OH-PBDE isomers, 6OH-BDE47 was predominant followed by 2'OH-BDE68 in Japanese amberjacks and scalloped hammerhead sharks. Residue levels of Sigma MeO-PBDEs and Sigma OH-PBDEs showed a significant positive correlation (p = 0.029). This result suggests that MeO-PBDEs and OH-PBDEs share a common source or a metabolic pathway in fishes. Characteristic differences found in the profiles of OH-PCBs and OH-PBDEs in Japanese amberjack and scalloped hammerhead shark show the need for further studies on the differences in exposure profiles, metabolic capacities and toxic effects in fish. (C) 2011 Elsevier Ltd. All rights reserved.
  • Organohalogen Contaminants and their Hydoxylated Metabolites in the Blood of Japanese Terrestrial Mammals.
    Mizukawa H, Nomiyama K, Nakatsu S, Yachimori S, Hayashi T, Tashiro Y, Tanabe S
    Organohalogen Compounds 73 1207 - 1210 2011年 [査読有り]
     
    Proceedings
  • Kurunthachalam Kannan, Shin Takahashi, Naohiro Fujiwara, Hazuki Mizukawa, Shinsuke Tanabe
    ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 58 4 901 - 907 2010年05月 [査読有り]
     研究論文(学術雑誌) 
    Organotin compounds (OTs) have been used in a wide variety of consumer products. Despite this, very few studies have reported the occurrence of OTs in house dust or exposure of humans to OTs through the ingestion of house dust. In the present study, concentrations of monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), monooctyltin (MOT), dioctyltin (DOT), trioctyltin (TOT), diphenyltin (DPT), and triphenyltin (TPT) were measured in dust collected from 24 houses in Albany, New York, USA. In addition, a few household products, such as wallpaper, floor tile, vinyl window blinds, and handbags were analyzed for the presence of OTs. Organotins were found in all of the house dust samples analyzed, and total OT concentrations varied from 390 to 28,000 ng/g (mean +/- A SD: 6700 +/- A 6200; median: 5000). Relative abundances of OTs in house dust were in the order MBT > MOT > DBT > DOT > TBT. TOT, DPT, and TPT were not found in any of the samples at concentrations above their corresponding detection limits. MBT accounted for, on average, 51% of the total OT concentrations. Mean concentrations of total OTs found in house dust samples from our study were two to five times higher than concentrations that have been reported for dust samples from several European countries. Calculations indicate that dust ingestion by children account for, on average, 15-18% of the tolerable daily intake proposed by the World Health Organization (WHO). The estimated rates of OT intake by children via dust ingestion were, on average, eightfold higher than the intake rates calculated for adults. Household products, such as wallpaper, contained total OT concentrations as high as 780,000 ng/g.
  • Kei Nomiyama, Satoko Murata, Tatsuya Kunisue, Tadasu K. Yamada, Hazuki Mizukawa, Shin Takahashi, Shinsuke Tanabe
    ENVIRONMENTAL SCIENCE & TECHNOLOGY 44 10 3732 - 3738 2010年05月 [査読有り]
     研究論文(学術雑誌) 
    In this study, we determined the residue levels and patterns of polychlorinated biphenyls (PCBs) and hydroxylated PCBs (OH-PCBs) in the blood from eight species of toothed whales and three species of baleen whales stranded along the Japanese coast during 1999-2007. Penta- through hepta-chlorinated PCB congeners were the dominant homologue groups in all cetaceans. In contrast, specific differences in the distribution of dominant OH-PCB isomers and homologues were found among the cetacean species. In five species of toothed whales (melon-headed whale, Stejneger's beaked whale, Pacific white-sided dolphin, Blainville's beaked whale, and killer whale), the predominant homologues were OH-penta-PCBs followed by OH-tetra-PCBs and OH-tri-PCBs. The predominant homologues of finless porpoise and beluga whale were OH-penta-PCBs followed by OH-hexa-PCBs and OH-tri-PCBs. The predominant OH-PCB isomers were para-OH-PCBs such as 4'OH-CB26, 4'OH-CB25/4'OH-CB26/4OH-CB31, 4OH-CB70, 4'OH-CB72, 4'OH-CB97, 4'OH-CB101/4'OH-CB120, and 4OH-CB107/4'OH-CB108 in toothed whales. In three baleen whales (common minke whale, Bryde's whale, and humpback whale) and in sperm whale (which is a toothed whale), OH-octa-PCB (4OH-CB202) was the predominant homologue group accounting for 40-80% of the total OH-PCB concentrations. The differences in concentrations and profiles of OH-PCBs may suggest species-specific diets, metabolic capability, and the transthyretin (TTR) binding specificity. These results reveal that the accumulation profiles of OH-PCBs in cetacean blood are entirely different from the profiles found in pinnipeds, polar bear, and humans.

講演・口頭発表等

  • ネコ用ウェットフードにおける有機ハロゲン化合物の汚染実態とリスク評価  [通常講演]
    佐藤楓夏, 島崎真琴, 水川葉月, 国末達也, 田辺信介, 野見山桂
    環境化学オンライン研究発表会2020 2020年09月 口頭発表(一般)
  • Species variations in cytochrome P450-mediated metabolism of neonicotinoids among cats, dogs, rats, and humans  [通常講演]
    Kraisiri Khidkhan, Yoshinori Ikenaka, Takahiro Ichise, Shouta M, M. Nakayama, Hazuki Mizukawa, Kei Nomiyama, Hisato Iwata, Koji Arizono, Keisuke Takahashi, Keisuke Kato, Mayumi Ishizuka
    環境化学オンライン研究発表会2020 2020年09月 口頭発表(一般)
  • 松山平野の河川におけるフィプロニルとその分解物の汚染実態解明と生態リスク評価  [通常講演]
    Hoa Thi Thuy Dung, 水川葉月, 高橋真
    環境化学オンライン研究発表会2020 2020年09月 口頭発表(一般)
  • 野生鳥類における残留性有機汚染物質の蓄積と経年変化  [通常講演]
    渡辺桃加, 水川葉月, 高橋真
    環境化学オンライン研究発表会2020 2020年09月 口頭発表(一般)
  • Species differences in cytochrome P450-mediated metabolism of polychlorinated biphenyls between cats and dogs  [招待講演]
    Khidkhan K, Mizukawa H, Shimasaki M, Nomiyama K, Ikenaka Y, Nakayama SMM, Iwata H, Ishizuka M
    International Symposium on Chemical Hazard in Wildlife 2020年02月 口頭発表(招待・特別)
  • Comprehensive analysis on halogenated phenolic compounds in wildlife and companion animals by the exploitation of the Environmental Specimen Bank (es-BANK) in Ehime University  [招待講演]
    Mizukawa H, Nomiyama K, Tanabe S
    International Symposium on Chemical Hazard in Wildlife 2020年02月 口頭発表(招待・特別)
  • ペット動物における有機ハロゲン化合物の汚染実態解明と曝露源の推定  [通常講演]
    水川葉月, 野見山桂
    2019年室内環境学会学術大会 2019年12月 口頭発表(一般) 沖縄県市町村自治会館、那覇市
  • ペット動物から見る室内化学物質汚染の実態解明とリスク評価  [通常講演]
    水川 葉月, 野見山 桂, 池中 良徳, 中山 翔太, 石塚 真由美, 高橋 真
    第22回環境ホルモン学会研究発表会 2019年12月 口頭発表(一般) 東京大学弥生講堂、東京都
  • 松山平野の河川水におけるフィプロニルとその分解物の汚染実態解明  [通常講演]
    Hoa Thi Thuy Dung, 水川 葉月, 高橋 真
    第22回環境ホルモン学会研究発表会 2019年12月 ポスター発表 東京大学弥生講堂、東京都
  • Investigation of metabolic capacities of organohalogen compounds and risk assessment in cats and dogs  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Hisato Iwata, Kraisiri Khidkhan, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta Nakayama, Shinsuke Tanabe
    3rd International Caparica Conference on Pollutant Toxic Ions and Molecules 2019 (PTIM2019) 2019年11月 口頭発表(一般) ALDEIA DOS CAPUCHOS GOLF & SPA、リスボン、ポルトガル
  • 体内動態および行動毒性試験から見た動物用医薬品フィプロニルの影響評価  [通常講演]
    鈴木智裕, 池中良徳, 中山翔太, 水川葉月, 一瀬貴大, 佐々木東, 笹岡一慶, 石塚真由美
    第162回日本獣医学会学術集会 2019年09月 口頭発表(一般) つくば国際会議場
  • Comparative metabolism of PCBs by cytochrome P450 in cat and dog  [通常講演]
    Kraisiri KHIDKHAN, Hazuki MIZUKAWA, Makoto SHIMASAKI, Kei NOMIYAMA, Yoshinori IKENAKA, Shouta M.M. NAKAYAMA, Hisato IWATA, Mayumi ISHIZUKA
    SaSSOH 2019 2019年09月 北海道大学獣医学部、札幌市
  • Elucidation of indoor pollution and risk assessment~Can pets be human sentinel animals? ~  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Yoshinori Ikenaka, Kraisiri Khidkhan, Shouta Nakayama, Mayumi Ishizuka
    3rd Chemical Hazard Symposium 2019年09月 口頭発表(一般) 北海道大学札幌キャンパス
  • Contaminations of Organohalogen Compounds in Pet Cats, Cat Food and House Dust from Thailand  [通常講演]
    Makoto Shimasaki, Hazuki Mizukawa, Kohki Takaguchi, Aksorn Saengtienchai, Araya Ngamchirttakul, Disdanai Pencharee, Kraisiri Khidkhan, Mayumi Ishizuka, Yoshinori Ikenaka, Shota M.M. Nakayama, Tatsuya Kunisue, Shinsuke Tanabe, Kei Nomiyama
    3rd Chemical Hazard Symposium 2019年09月 口頭発表(一般) 北海道大学札幌キャンパス
  • Temporal trends and congener profiles of PCBs in a dated sediment core from Beppu Bay, Japan  [通常講演]
    Daichi AONO, Hoang Q. ANH, Isao WATANABE, Keidai TOMIOKA, Hazuki MIZUKAWA, Michinobu KUWAE, Shin TAKAHASHI
    3rd Chemical Hazard Symposium 2019年09月 口頭発表(一般) 北海道大学札幌キャンパス
  • Concentrations and congener patterns of organohalogen compounds in cat food  [通常講演]
    Makoto Shimasaki, Hazuki Mizukawa, Aksorn Saengtienchai, Khidkhan Kraisiri, Yoshinori Ikenaka, Shota MM Nakayama, Mayumi Ishizuka, Kei Nomiyama
    39th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2019 2019年08月 ポスター発表 ICC Kyoto, Kyoto 
    Program, 1-PS-271
  • Effects related to thyroid hormones disruption by organohalogen compounds in the pet's cat (Felis catus) serum using a metabolomics approach  [通常講演]
    Kei Nomiyama, Akifumi Eguchi, Hazuki Mizukawa, Yasuo Yamamoto, Hiroyuki Nishikawa, Mitsuyoshi Takiguchi, Shouta M.M. Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka, Shinsuke Tanabe
    39th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2019 2019年08月 ポスター発表 ICC Kyoto, Kyoto 
    Program, 2-PS-272
  • PCBs effects on comprehensive gene expression in cats and dogs  [通常講演]
    Hazuki Mizukawa, Hisato Iwata, Hoa Thanh Ngyuen, Kraisiri Khidkhan, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    39th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2019 2019年08月 口頭発表(一般) ICC Kyoto, Kyoto 
    Program, 4C2-PM2-01
  • Contamination status of organohalogen compounds in pet cats, cat food and house dust from Thailand  [通常講演]
    Makoto Shimasaki, Hazuki Mizukawa, Aksorn Saengtienchai, Kei Nomiyama
    39th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2019 2019年08月 口頭発表(一般) ICC Kyoto, Kyoto 
    Program, 4C2-PM2-02
  • Plastic debris moves chemical additives to seabird tissue  [通常講演]
    Kosuke Tanaka, Yutaka Watanuki, Hideshige Takada, Mayumi Ishizuka, Rei Yamashita, Mami Kazama, Nagako Hiki, Kaoruko Mizukawa, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta MM Nakayama
    39th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2019 2019年08月 ポスター発表 ICC Kyoto, Kyoto 
    Program, 3-PS-219
  • 摂食プラスチックから海鳥への化学物質の移行の検証  [通常講演]
    田中厚資, 綿貫豊, 高田秀重, 石塚真由美, 山下麗, 水川薫子, 水川葉月, 池中良徳, 中山翔太
    第28回環境化学討論会 2019年06月 ポスター発表 埼玉会館、さいたま市
  • デカブロモジフェニルエーテル(BDE209)の長期曝露がイエネコへ及ぼす影響  [通常講演]
    田中啓介, 野見山桂, 水川葉月, 髙口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第28回環境化学討論会 2019年06月 口頭発表(一般) 埼玉会館、さいたま市
  • PCBs 曝露によるネコ肝トランスクリプトームへの影響  [通常講演]
    水川葉月, Hoa Thanh Ngyuen, 岩田久人, 野見山桂, 池中良徳, 中山翔太, 横山望, 市居修, 滝口満喜, Kraisiri Khidkhan, 田辺信介, 石塚真由美
    第28回環境化学討論会 2019年06月 口頭発表(一般) 埼玉会館、さいたま市
  • Contamination Status of Organohalogen Compounds in Pet Cats, Cat Food and House Dust from Thailand  [通常講演]
    Makoto Shimasaki, Hazuki Mizukawa, Kohki Takaguchi, Aksorn Saengtienchai, Araya Ngamchirttakul, Disdanai Pencharee, Kraisiri Khidkhan, Mayumi Ishizuka, Yoshinori Ikenaka, Shota M.M. Nakayama, Tatsuya Kunisue, Shinsuke Tanabe, Kei Nomiyama
    第28回環境化学討論会 2019年06月 口頭発表(一般) 埼玉会館、さいたま市
  • LC-MS/MS を用いたイヌ血清中ステロイドホルモンの分析法の確立と副腎疾患モデルを用いた実測  [通常講演]
    白尾大樹, 池中 良徳, 一瀬 貴大, 中山 翔太, 佐々木東, 永田 矩之, 水川葉月, 滝口満善, 石塚 真由美
    第28回環境化学討論会 2019年06月 口頭発表(一般) 埼玉会館、さいたま市
  • Comparison of effects on comprehensive gene expression by PCBs exposure in Cats and Dogs  [通常講演]
    Hazuki Mizukawa, Hisato Iwata, Hoa Thanh Ngyuen, Saya Tamura, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kei Nomiyama, Mayumi Ishizuka
    2nd Chemical Hazard Symposium 2018年12月 口頭発表(一般) Ehime University
  • Monitoring of blood lead levels in dogs from lead pollution area, Kabwe, Zambia  [通常講演]
    Haruya Toyomaki, Shouta M.M, Nakayama, John Yabe, Yared B. Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    2nd Chemical Hazard Symposium 2018年12月 口頭発表(一般) Ehime University
  • Characterization of CYP genes related-PCBs exposure in cats  [通常講演]
    Kraisiri Khidkhan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M, M. Nakayama, Kei Nomiyama, Wageh Sobhy Darwish, Osamu Ichii, Nozomu Yokoyama, Mitsuyoshi Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    2nd Chemical Hazard Symposium 2018年12月 口頭発表(一般) Ehime University
  • Uncertainties Associated with Neonicotinoid Insecticide`s Safety Thresholds and the Potential Ramifications on Human Health: A Case Study of Ghana  [通常講演]
    Collins NIMAKO, Yoshinori IKENAKA, Osei AKOTO, Nesta BORTEY-SAM, Shouta M.M. NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    2nd Chemical Hazard Symposium 2018年12月 口頭発表(一般) Ehime University
  • Effects of PCB exposure on thyroid hormone levels in serum of dogs and cats.  [通常講演]
    Kohki Takaguchi, Hiroyuki Nishikawa, Hazuki Mizukawa, Rumi Tanoue, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shouta M, M. Nakayama, Yoshinori Ikenaka, Tatsuya Kunisue, Mayumi Ishizuka, Shinsuke Tanabe, Hisato Iwata, Kei Nomiyama
    SETAC North America 39th Annual Meeting 2018年11月 ポスター発表 Sacramento Convention Center, CA, USA
  • Avian interspecific differences in rodenticide sensitivity: amino acid sequence and mRNA expression ratio of VKORC1 and VKORC1L1  [通常講演]
    Shouta M.M. NAKAYAMA, Ayuko MORITA, Yoshinori IKENAKA, Yusuke K. KAWAI, Kazuki TAKEDA, Hazuki MIZUKAWA, Chihiro ISHII, Keisuke SAITO, Yukiko WATANABE, Masaki ITO, Natsuo Ohsawa, Mayumi ISHIZUKA
    SETAC North America 39th Annual Meeting 2018年11月 口頭発表(一般) Sacramento Convention Center, CA, USA
  • Blood Lead (Pb) Levels and Stable Isotope Ratio among Children, Infants and Mothers in Kabwe, Zambia.  [通常講演]
    Shouta MM Nakayama, John Yabe, Haruya Toyomaki, Kaampwe Muzandu, Yared B Yohannes, Hokuto Nakata, Yoshinori Ikenaka, Hazuki Mizukawa, Takeshi Kuritani, Mitsuhiro Nakagawa, Mayumi Ishizuka
    SETAC North America 39th Annual Meeting 2018年11月 ポスター発表 Sacramento Convention Center, CA, USA
  • Avian interspecific differences in VKOR activity and inhibition: Amino acid sequence and mRNA expression ratio of VKORC1 and VKORC1L1.  [通常講演]
    Shouta M.M. NAKAYAMA, Ayuko MORITA, Yoshinori IKENAKA, Yusuke K. KAWAI, Kazuki TAKEDA, Hazuki MIZUKAWA, Chihiro ISHII, Keisuke SAITO, Yukiko WATANABE, Masaki ITO, Natsuo Ohsawa, Mayumi ISHIZUKA
    2018 International Meeting on 22nd MDO and 33rd JSSX 2018年10月 ポスター発表 Ishikawa Ongakudo Hall, Kanazawa
  • Inter-species differences of phase II xenobiotics metabolism enzymes in carnivorans.  [通常講演]
    Takamitsu Kondo, Yoshinori Ikenaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Yoko Mitani, Kei Nomiyama, Shinsuke Tanabe, Mayumi Ishizuka
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 口頭発表(一般) Hokkaido University
  • Vegetation status and suppression of Pb accumulation in wild lizards around Pb-Zn mine in Kabwe, Zambia.  [通常講演]
    Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Yared Beyene, Andrew Kataba, Golden Zyambo, Yoshitaka Uchida, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 ポスター発表 Hokkaido University
  • Does dog’s behavior affect the blood lead levels in domestic dogs around lead mining area, Kabwe, Zambia?  [通常講演]
    Haruya Toyomaki, John Yabe, Shouta M.M. Nakayama, Yared Beyene Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Mayumi Ishizuka
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 口頭発表(一般) Hokkaido University
  • Effects of zinc co-administration with lead (Pb) on Pb biodistribution in tissues of Sprague Dawley (SD) rats.  [通常講演]
    Andrew Kataba, Shouta M.M Nakayama, Yared B. Yohannes, Haruya Toyomaki, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 ポスター発表 Hokkaido University
  • DNA Methylation Biomarkers in Environmental Lead-Exposed Children from Kabwe, Zambia.  [通常講演]
    Yared Beyene Yohannea, Shouta M.M, Nakayama, John Yabe, Toyomaki Haruya, Kaampwe Muzandu, Hokuto Nakata, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 ポスター発表 Hokkaido University
  • Evaluation of Exposure and Toxicological Implications of Neonicotinoid Insecticides among the residents of Kumasi, Ghana.  [通常講演]
    Collins NIMAKO, Yoshinori IKENAKA, Osei AKOTO, Nesta BORTEY-SAM, Shouta M.M.NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 ポスター発表 Hokkaido University
  • Charcterization of CYP expression in cat-related PCBs exposure.  [通常講演]
    Kraisiri Khidkan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M, M. Nakayama, Wageh Sobhy Darwish, Kei Nomiyama, Mitsuyoshi Takiguchi, Nozomu Yokoyama, Osamu Ichii, Shinsuke Tanabe, Mayumi Ishizuka
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 ポスター発表 Hokkaido University
  • Assessment of vitamin K epoxide reductase by in vitro kinetic analysis & in silico docking simulation for evaluation of rodenticide sensitivity.  [通常講演]
    Kazuki TAKEDA, Yoshinori IKENAKA, Kazuyuki D. TANAKA, Shouta M.M. NAKAYAMA, Tsutomu TANIKAWA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    The 6th Sapporo Summer Symposium for One Health (6th SaSSOH) 2018年09月 ポスター発表 Hokkaido University
  • Evaluation of Neonicotinoid Insecticide Exposure and Toxicity among some residents of Kumasi, Ghana.  [通常講演]
    Collins NIMAKO, Yoshinori IKENAKA, Osei AKOTO, Nesta BORTEY-SAM, Shouta M.M. NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    Society for Environmental Toxicology and Chemistry, Asia-Pacifi 2018 (SETAC-AP2018) 2018年09月 ポスター発表 EXCO, Daegu, South Korea
  • グルクロン酸転移酵素UGT1A6と哺乳類の食性の関係  [通常講演]
    佐野花澄, 池中良徳, 近藤誉充, 川合佑典, 中山翔太, 水川葉月, 石塚真由美
    第161回日本獣医学会学術集会 2018年09月 口頭発表(一般) つくば国際会議場
  • オジロワシを中心とした鳥類の食性による腸内細菌叢変動と影響の解析  [通常講演]
    山田尚輝, 中山翔太, 水川葉月, 小笠原浩平, 渡邊有希子, 齊藤慶輔, 伊藤真輝, 大澤夏生, 竹中康進, 小松謙之, 千葉茜, 内田義崇, 池中良徳, 石塚真由美
    第161回日本獣医学会学術集会 2018年09月 口頭発表(一般) つくば国際会議場
  • 誘導体化液体クロマトグラフィー質量分析法(LC/MS法)によるエクイリン・エクイレニンを中心とするステロイドホルモン多成分一斉分析メソッドの開発及びその応用  [通常講演]
    三津橋嵩史, 池中良徳, 一瀬貴大, Aksorn Saengtienchai, 中山翔太, 水川葉月, 河合正人, 南保泰雄, 石塚真由美
    第161回日本獣医学会学術集会 2018年09月 口頭発表(一般) つくば国際会議場
  • Contamination status and global comparison of organohalogen compounds and their related compounds in the pet cats from Thailand.  [通常講演]
    Makoto Shimasaki, Kei Nomiyama, Hazuki Mizukawa, Aksorn Saengtienchai, Araya Ngamchirttakul, Disdanai Pencharee, Yoshinori Ikenaka, Shouta MM Nakayama, Tatsuya Kunisue, Shinsuke Tanabe
    38th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2018 2018年08月 ポスター発表 ICE Krakow Congress Centre, Krakow, Poland
  • Pilot study on the toxicological assessment of organohalogen compounds in pet cat (Felis catus) serum using metabolomics approach.  [通常講演]
    Kei Nomiyama, Akifumi Eguchi, HazukiMizukawa, Yasuo Yamamoto, Hiroyuki Nishikawa, Mitsuyoshi Takiguchi, Shouta MM Nakayama, Yoshinori Ikenaka, Mayumi Isizuka, Shinsuke Tanabe
    38th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2018 2018年08月 口頭発表(一般) ICE Krakow Congress Centre, Krakow, Poland
  • Effects of Polychlorinated Biphenyls (PCBs) in Cats: Exhaustive Gene Expression Analysis Approach.  [通常講演]
    Hazuki Mizukawa, Kraisiri Khidkhan, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Sainnoxoi Tsend-ayush, Shinsuke Tanabe, Mayumi Ishizuka
    38th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2018 2018年08月 口頭発表(一般) ICE Krakow Congress Centre, Krakow, Poland
  • Tissue distribution and CYP expression related-PCBs exposure in cats.  [通常講演]
    Kraisiri Khidkan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M, M. Nakayama, Wageh Sobhy Darwish, Kei Nomiyama, Mitsuyoshi Takiguchi, Nozomu Yokoyama, Osamu Ichii, Shinsuke Tanabe, Mayumi Ishizuka
    38th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : DIOXIN 2018 2018年08月 口頭発表(一般) ICE Krakow Congress Centre, Krakow, Poland
  • The estimation of potential PCBs toxicity in cats by evaluating CYP450 expressions.  [通常講演]
    Kraisiri Khidkan, Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M, M. Nakayama, Kei Nomiyama, Mitsuyoshi Takiguchi, Nozomu Yokoyama, Osamu Ichii, Shinsuke Tanabe, Mayumi Ishizuka
    38th International Symposium on Halogenated Persistent Organic Pollutants (POPs) : Pre- Dioxin 2018 Students’ Symposium 2018年08月 ポスター発表 Jagiellonian University, Krak?w, Poland
  • Organohalogens metabolic capacities and their toxicological risk in cats  [招待講演]
    Hazuki Mizukawa
    MiSSE (Mixture assessment of endocrine disrupting compounds) Project Final Conference2018 2018年08月 口頭発表(招待・特別) Stockholm, Sweden
  • Importance of Vegetation in Reducing Pb Accumulation in Wild Lizards around a Pb-Zn Mine in Kabwe, Zambia.  [通常講演]
    Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Yared Beyene, Andrew Kataba, Golden Zyambo, Yoshitaka Uchida, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting. 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • Monitoring of Dog’s Behavior using GPS and their blood lead levels around lead mining area.  [通常講演]
    Haruya Toyomaki, John Yabe, Shouta M.M. Nakayama, Yared Beyene Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting. 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • Relationship between blood lead levels in infants and mothers around lead mining area, Kabwe, Zambia.  [通常講演]
    Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Shouta M.M. Nakayama, Yared Beyene Yohannes, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Takeshi Kuritani, Mitsuhiro Nakagawa, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting. 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • Ameliorative effect of Moringa Oleifera on Pb-induced toxicity in rat  [通常講演]
    Hokuto Nakata, Andrew Kataba, Haruya Toyomaki, Rio Doya, Shouta M.M, Nakayama, John Yabe, Kaampwe Muzandu, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • The trend of elevated blood lead levels in Kabwe residents. 2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting  [通常講演]
    Hokuto Nakata, John Yabe, Shouta M.M. Nakayama, Kaampwe Muzandu, Yared Beyene Yohannes, Haruya Toyomaki, Hazuki Mizukawa, Yoshinori Ikenaka, Russel Dowling, Jack Caravanos, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • Effects of zinc (Zn) supplementation on lead (Pb) accumulation in tissues of Sprague Dawley (SD) rats.  [通常講演]
    Andrew Kataba, Shouta M.M Nakayama, Yared B. Yohannes, Haruya Toyomaki, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • Gene Polymorphism and DNA Methylation Patterns of Lead Biomarker Gene in Environmental Lead Exposed Children from Kabwe, Zambia.  [通常講演]
    Yared Beyene Yohannes, Shouta M.M, Nakayama, John Yabe, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Hokuto Nakata, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) reveals distribution of Pb in mice organs  [通常講演]
    Shouta M.M. Nakayama, Masao Togao, Yoshinori Ikenaka, Hazuki Mizukawa, Yoshiki Makino, Ayano Kubota, Takehisa Matsukawa, Kazuhito Yokoyama, Takafumi Hirata, Mayumi Ishizuka
    2nd International KAMPAI Symposium & JST Mid-term Evaluation Meeting 2018年08月 ポスター発表 InterContinental Lusaka, Lusaka, Zambia
  • 国内に生息する野生鳥類における鉛曝露状況の解明  [通常講演]
    石井 千尋, 池中 良徳, 中山 翔太, 齊藤 慶輔, 渡辺 有希子, 小笠原 浩平, 水川 葉月, 石塚 真由美
    第24回日本野生動物医学会大会 2018年08月 ポスター発表 大阪府立大学 りんくうキャンパス
  • 鰭脚類を中心とした食肉目の薬物代謝酵素である硫酸転移酵素の種差解明  [通常講演]
    近藤 誉充, 池中 良徳, 中山 翔太, 水川 葉月, 三谷 曜子, 田辺 信介, 野見山 桂, 石塚 真由美
    第24回日本野生動物医学会大会 2018年08月 ポスター発表 大阪府立大学 りんくうキャンパス
  • 殺鼠剤に弱い野生動物種を探る:標的分子ビタミンKエポキシド還元酵素(VKOR)の鳥類における特徴および種差  [通常講演]
    中山翔太, 森田鮎子, 池中良徳, 石井千尋, 水川葉月, 武田一貴, 川合佑典, 齊藤慶輔, 渡邊有希子, 大澤夏生, 伊藤真輝, 石塚真由美
    第24回日本野生動物医学会大会 2018年08月 ポスター発表 大阪府立大学 りんくうキャンパス
  • PCR法による鳥類の雌雄判定:孵化前雌雄判定の試みとその課題  [通常講演]
    中山翔太, 池中良徳, 石井千尋, 水川葉月, 川合佑典, 平野なぎ沙, 齊藤慶輔, 渡邊有希子, 大澤夏生, 伊藤真輝, 中村亮平, 石塚真由美
    第24回日本野生動物医学会大会 2018年08月 ポスター発表 大阪府立大学 りんくうキャンパス
  • 食肉目動物での第II 相抱合酵素の遺伝的性状および酵素学的性状の解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美
    第45回日本毒性学会学術年会 2018年07月 口頭発表(一般) 大阪国際会議場(グランキューブ大阪)、大阪
  • スーパーラットは何が「スーパー」なのか? 〜東京の殺鼠剤抵抗性クマネズミにおける抵抗性獲得機序の探索〜  [通常講演]
    武田 一貴, 池中 良徳, 田中 和之, 中山 翔太, 谷川 力, 水川 葉月, 石塚 真由美
    第45回日本毒性学会学術年会 2018年07月 ポスター発表 大阪国際会議場(グランキューブ大阪)、大阪
  • ザンビア共和国カブウェ鉱床地域における鉛汚染問題:10年間の研究結果と今後の課題  [通常講演]
    中山翔太, John Yabe, 池中良徳, Kaampwe Muzandu, Kennedy Choongo, 中田北斗, 豊巻治也, Yared Beyene, Andrew Kataba, Golden Zyambo, 栗谷豪, 中川光弘, 水川葉月, 石塚真由美
    第45回日本毒性学会学術年会 2018年07月 口頭発表(一般) 大阪国際会議場(グランキューブ大阪)、大阪
  • ポリ塩化ビフェニル曝露によるイヌ・ネコの甲状腺ホルモン恒常性への影響  [通常講演]
    髙口倖暉, 野見山桂, 西川博之, 水川葉月, 田上瑠美, 横山望, 市居修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 岩田久人, 国末達也, 田辺信介
    第45回日本毒性学会学術年会 2018年07月 ポスター発表 大阪国際会議場(グランキューブ大阪)、大阪
  • KAbwe Mine Pollution Amelioration Initiative ~KAMPAI~  [通常講演]
    Shouta M.M. NAKAYAMA, Yoshinori IKENAKA, Hokuto NAKATA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    2018(平成30)年度 海外学術調査フォーラム 2018年06月 ポスター発表 東京外国語大学アジア・アフリカ言語文化研究所
  • Utility evaluation for stable Lead isotope ratios for source identification and verifying biological fractionation of lead isotopes in goats and chickens  [招待講演]
    Hokuto Nakata, Shouta M.M, Nakayama, John Yabe, Allan Liazambi, Hazuki Mizukawa, Wageh Sobhy Darwish, Yoshinori Ikenaka, Mayumi Ishizuka
    7th Zambia Water Forum and Exhibition 2018年06月 口頭発表(招待・特別) Government Complex, Lusaka, Zambia
  • Distribution of Pb in mice organs using bioimaging by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)  [招待講演]
    Shouta M.M. Nakayama, Masao Togao, Yoshinori Ikenaka, Hazuki Mizukawa, Yoshiki Makino, Ayano Kubota, Takehisa Matsukawa, Kazuhito Yokoyama, Takafumi Hirata, Mayumi Ishizuka
    7th Zambia Water Forum and Exhibition 2018年06月 口頭発表(招待・特別) Government Complex, Lusaka, Zambia
  • Trace Elemental Profiles of Serum and Cerebrospinal Fluid in Dogs with Idiopathic Epilepsy  [通常講演]
    Kazuyoshi Sasaoka, Hiroshi Ohta, Hazuki Mizukawa, Shouta Nakayama, Noboru Sasaki, Keitaro Morishita, Yoshinori Ikenaka, Mayumi Ishizuka, Mitsuyoshi Takiguchi
    2018 ACVIM Forum (American College of Veterinary Internal Medicine) 2018年06月 ポスター発表 Seattle, Washington, USA
  • The trend of elevated blood lead levels in mining area, Kabwe, Zambia  [通常講演]
    Hokuto Nakata, Shouta M.M, Nakayama, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Haruya Toyomaki, Andrew Kataba, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Russell Dowling, Jack Caravanos, Mayumi Ishizuka
    日本アフリカ学会第55回学術大会 2018年05月 口頭発表(一般) 北海道大学学術交流会館
  • Analysis of the amino acid metabolome in p,p’-DDT exposed chickens  [通常講演]
    Lesa A Thompson, Isabel M Vincent, Yoshinori Ikenaka, Stefan K Weidt, Wageh S Darwish, Shouta MM Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • マラリアベクターコントロールにおけるDDTの使用実態とリスク評価  [通常講演]
    池中良徳, リサ トンプソン, ダルウィッシュ ワギ, ヨハネス ヤレド, ファンファーレン ヨハン, スミッツ ニコ, ヴェペナー ヴィクター, 中山翔太, 水川葉月, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • ネオニコチノイド系殺虫剤のヒト健康影響評価問題点の整理と今後の研究課題  [通常講演]
    池中良徳, 一瀬貴大, ニマコ コリンズ, 市川剛, 水川葉月, 中山翔太, 有薗幸司, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • 誘導体化LC/MS/MS法による血漿中のステロイドホルモンおよびその抱合体代謝物35種類の一斉分析法の開発とその応用  [通常講演]
    一瀬貴大, 池中良徳, Aksorn Saengtienchai, 中山翔太, 水川葉月, 石塚真由美
    第27回環境化学討論会 2018年05月 ポスター発表 沖縄県市町村自治会館、那覇市、沖縄県
  • 野生哺乳類における硫酸転移酵素の動物種差解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • タイ王国におけるペットネコ血清およびペットフード中有機ハロゲン化合物と類縁化合物の汚染実態  [通常講演]
    島崎真琴, 野見山桂, 水川葉月, Aksorn Saengtienchai, Araya Ngamchirttakul, Disdanai Pencharee, 石塚真由美, 池中良徳, 中山翔太, 国末達也, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • 血清中メタボローム解析によるペットネコ(Felis catus)の有機ハロゲン化合物毒性影響評価  [通常講演]
    野見山桂, 江口哲史, 水川葉月, 山本恭央, 西川博之, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • ネコのin vivo BDE209長期曝露試験による体内動態の解明および甲状腺ホルモンへの影響評価  [通常講演]
    田中啓介, 野見山桂, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • 有機ハロゲン化合物曝露によるネコの生化学マーカーの変化および甲状腺機能に及ぼす影響評価  [通常講演]
    水川葉月, Kraisiri Khidkhan, 高口倖暉, 横山望, 市居修, 滝口満喜, 笹岡一慶, 池中良徳, 中山翔太, 野見山桂, 田辺信介, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • Characteristics of CYP450 expression in cat exposed PCBs: Estimating the potential toxicity  [通常講演]
    Kraisiri Khidkhan, Hazuki Mizukawa, Kei Nomiyama, Yoshinori Ikenaka, Shota M, M. Nakayama, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Hiroyuki Nishikawa, Keisuke Tanaka, Kohki Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • HPLC-APCI-MSを用いたビタミンKの網羅的検出とin vitroビタミンKエポキシド還元酵素活性の評価  [通常講演]
    武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • 鉛鉱床地域における野生トカゲ亜目の生体内金属類濃度に影響を与える環境要因の解析  [通常講演]
    銅谷理緒, 中山翔太, 中田北斗, 豊巻治也, Yabe John, Muzandu Kaampwe, Beyene Yared, Kataba Andrew, yambo Golden, 水川 葉月, 池中 良徳, 石塚 真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • GPS装置を用いた鉛鉱床地域のイヌの行動のモニタリングと鉛曝露の関係性  [通常講演]
    豊巻治也, Yabe John, 中山翔太, Beyene Yared, 水川葉月, 池中良徳, 中田北斗, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • Utility of Pb isotopes for source identification and species differences of Pb isotope biological fractionation  [通常講演]
    Hokuto Nakata, Shouta M.M, Nakayama, John Yabe, Allan Liazambi, Hazuki Mizukawa, Wageh S. Darwish, Yoshinori Ikenaka, Mayumi Ishizuka
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • Evaluation of Neonicotinoid Insecticide exposure in humans living in the Kumasi city, Ghana  [通常講演]
    Collins Nimako, Yoshinori Ikenaka, Osei Akoto, Nesta Bortey-Sam, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • Lead (Pb) accumulation in tissues of Sprague Dawley (SD) rats following sub chronic Lead-Zinc exposure  [通常講演]
    Andrew Kataba, Shouta Nakayama, Yared B. Yohannes, Haruya Toyomaki, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • Blood Lead Levels Among the General Population in Kabwe, Zambia  [通常講演]
    Yared B. Yohannes, Shouta Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Kaampwe Muzandu, Andrew Kataba, Yoshinori Ikenaka, Hazuki Mizukawa, Russell Dowling, Jack Caravanos, Mayumi Ishizuka
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • 非侵襲的に採取可能な乳幼児の糞便における鉛安定同位体比を用いた鉛暴露経路の推定  [通常講演]
    中山翔太, 豊巻治也, John Yabe, Kaampwe Muzandu, 中田北斗, 栗谷豪, 中川光弘, 水川葉月, 池中良徳, 石塚真由美
    第27回環境化学討論会 2018年05月 口頭発表(一般) 沖縄県市町村自治会館、那覇市、沖縄県
  • 放射能高濃度汚染地区を水源とする河川による水田汚染の状況  [通常講演]
    仲井邦彦, 上野大介, 水川葉月, 長坂洋光, 水谷太, 苣木洋一, 相場俊樹, 大葉隆, 龍田希, 西浜柚季子
    第88回日本衛生学会総会 2018年03月 ポスター発表 東京工科大学蒲田キャンパス、東京
  • クロミンククジラ未成熟雌のヒゲ板安定同位体比を用いた摂餌履歴推定  [通常講演]
    打田茉由果, 鈴木一平, 石塚真由美, 池中良徳, 中山翔太, 水川葉月, 田村 力, 坂東武治, 小西健志, 三谷曜子
    平成30年度日本水産学会春季大会 2018年03月 口頭発表(一般) 東京海洋大学品川キャンパス、東京
  • Novel Mechanism of Rodenticide (warfarin) Resistance of Wild Rats in Tokyo ~ Enhanced Pentose Phosphate Pathway Causes Rapid Metabolism of Warfarin ~  [通常講演]
    Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    Society of Toxicology’s 57th Annual Meeting and ToxExpo 2018年03月 ポスター発表 Henry B. Gonzalez Convention Center, San Antonio, Texas, USA
  • Lead levels in eggs in Kabwe, Zambia: implication of human exposure  [通常講演]
    Haruya Toyomaki, John Yabe, Shouta M.M. Nakayama, Yared B. Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Takeshi Kuritani, Mitsuhiro Nakagawa, Mayumi Ishizuka
    Society of Toxicology’s 57th Annual Meeting and ToxExpo 2018年03月 ポスター発表 Henry B. Gonzalez Convention Center, San Antonio, Texas, USA
  • Migratory seabirds are responsible for mercury biotransport from marine to terrestrial ecosystems  [通常講演]
    Akiko Shoji, K. H. Elliott, S. Aris-Brosou, Hazuki Mizukawa, Shouta Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka, T. Kuwae, K. Watanabe, Yutaka Watanuki
    The 45th Pacific Seabird Group Annual Meeting 2018年02月 口頭発表(一般) Autonomous University of Baja California Sur, La Paz, Baja California Sur, Mexico
  • ザンビア・カブエ鉱床における鉛汚染問題とその解決に向けた取り組み-KAbwe Mine Pollution Amelioration Initiative (KAMPAI Project)  [通常講演]
    中山翔太, 水川葉月, 池中良徳, 石塚真由美
    第3回 北大部局間横断シンポジウム 2018年01月 口頭発表(一般) 北海道大学医学部学友会館フラテホール、北海道
  • スーパーラットは高NADPH産生能を持つ〜東京の殺鼠剤抵抗性ラットにおける抵抗性獲得機序の解明〜  [通常講演]
    武田一貴, 池中良徳, 田中和之, 谷川力, 中山翔太, 水川葉月, 石塚真由美
    第3回 北大部局間横断シンポジウム 2018年01月 ポスター発表 北海道大学医学部学友会館フラテホール、北海道
  • Evaluation of indoor residual spraying DDT for mosquito control: Tissue distribution and gene expression profiling in rat tissues  [通常講演]
    Emmanuel Temiotan, Ogbomida, Alex Ajeh Enuneku, Yoshinori Ikenaka, Yared Beyene Yohannes, Wageh Sobhy Darwish, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    環境ホルモン学会第20回研究発表会 2017年12月 ポスター発表 神戸大学、神戸市、兵庫県
  • 幼児および新生児におけるネオニコチノイド系殺虫剤の曝露実態  [通常講演]
    池中良徳, 宮原裕一, 一瀬貴大, 中山翔太, 水川葉月, 市川剛, 遠山千春, 石塚真由美
    環境ホルモン学会第20回研究発表会 2017年12月 口頭発表(一般) 神戸大学、神戸市、兵庫県
  • 東京のワルファリン抵抗性ラットの抵抗性獲得機序の解明:ペントースリン酸経路の亢進を通したワルファリン代謝の活性化 Investigation of mechanism of warfarin resistance of wild rats in Tokyo ~ enhanced pentose phosphate pathway causes rapid metabolism of warfarin ~  [通常講演]
    Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    2017年度生命科学系学会合同年次大会(ConBio2017) 2017年12月 口頭発表(一般) 神戸ポートアイランド、神戸市、兵庫県
  • ポリ塩化ビフェニル(PCBs)がネコの甲状腺機能に及ぼす影響  [通常講演]
    水川葉月, 高居名菜, 野見山桂, 高口倖暉, 横山望, 市居修, 滝口満喜, 池中良徳, 中山翔太, 石塚真由美
    環境ホルモン学会第20回研究発表会 2017年12月 ポスター発表 神戸大学、神戸市、兵庫県
  • Reliability of stable lead isotope analysis for lead source identification and verifying biological fractionation of lead isotopes in goats and chicken  [通常講演]
    Hokuto Nakata, Shouta M.M, Nakayama, John Yabe, Allan Liazambi, Hazuki Mizukawa, Wageh S Darwish, Yoshinori Ikenaka, Mayumi Ishizuka
    1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century 2017年11月 ポスター発表 Hokkaido University, Sapporo, Japan
  • Extent of lead exposure in mothers and infants living around a “closed” lead mining area, Kabwe, Zambia  [通常講演]
    Haruya Toyomaki, John Yabe, Shouta M.M. Nakayama, Yared B. Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Russell Dowling, Jack Caravanos, Mayumi Ishizuka
    1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century 2017年11月 口頭発表(一般) Hokkaido University, Sapporo, Japan
  • Current trends of blood lead levels in Kabwe, Zambia  [通常講演]
    Yared Beyene Yohannes, John Yabe, Shota M.M. Nakayama, Haruya Toyomaki, Hokuto Nakata, Kaampwe Muzandu, Andrew Kataba, Golden Zyambo, Hazuki Mizukawa, Yoshinori Ikenaka, Russell Dowling, Jack Caravanos, Mayumi Ishizuka
    1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century 2017年11月 口頭発表(一般) Hokkaido University, Sapporo, Japan
  • Local distribution of Pb in mice using bioimaging by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)  [通常講演]
    Shouta MM Nakayama, Masao Togao, Yoshinori Ikenaka, Hazuki Mizukawa, Yoshiki Makino, Ayano Kubota, Takehisa Matsukawa, Kazuhito Yokoyama, Takafumi Hirata, Mayumi Ishizuka
    1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century 2017年11月 ポスター発表 Hokkaido University, Sapporo, Japan
  • Elevated Blood Lead Levels among Children, Infants and Mothers in Kabwe, Zambia  [通常講演]
    Yared Beyene Yohannes, John Yabe, Shouta M.M. Nakayama, Haruya Totomaki, Kaampwe Muzandu, Yoshinori Ikenaka, Hazuki Mizukawa, Russell Dowling, Jack Caravanos, Hokuto Nakata, Mayumi Ishizuka
    SETAC North America 38th Annual Meeting 2017年11月 口頭発表(一般) Minneapolis Convention Center, Minneapolis, Minnesota, USA
  • Biochemical Alterations due to PCBs and PBDEs Exposures in Cats  [通常講演]
    Kraisiri Khidkhan, Hazuki Mizukawa, Kei Nomiyama, Yoshinori Ikenaka, Shouta M, M. Nakayama, Nozomu Yokoyama, Kazuyoshi Sasaoka, Osamu Ichii, Mitsuyoshi Takiguchi, Hiroyuki Nishikawa, Keisuke Tanaka, Kohki Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    SETAC North America 38th Annual Meeting 2017年11月 ポスター発表 Minneapolis Convention Center, Minneapolis, Minnesota, USA
  • Evaluation of utility for stable lead isotope ratios to identify lead sources and verifying biological fractionation of lead isotopes in goats and chickens  [通常講演]
    Hokuto Nakata, Shouta M.M, Nakayama, John Yabe, Allan Liazambi, Hazuki Mizukawa, Wageh Sobhy Darwish, Yoshinori Ikenaka, Mayumi Ishizuka
    SETAC North America 38th Annual Meeting 2017年11月 ポスター発表 Minneapolis Convention Center, Minneapolis, Minnesota, USA
  • Discovery of renal biomarkers in birds by transcriptome analysis and glycomics analysis following kidney injury in chicken model  [通常講演]
    Chihiro Ishii, Yoshinori Ikenaka, Yusuke, K. Kawai, Osamu Ichii, Shouta. M, M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    The 10th International Meeting of the Asian Society of Conservation Medicine (ASCM)、“One Health in Asia Pacific” 2017年10月 ポスター発表 Kuching、Sarawak、Malaysia
  • Effect of DDTs on gallinacins in the avian immune system  [通常講演]
    Lesa Thompson, Yoshinori Ikenaka, Yared Yohannes、Johan van Vuren、Victor Wepener, Nico Smit, Atnafu Assefa, Wageh Sobhy Darwish, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    The 10th International Meeting of the Asian Society of Conservation Medicine (ASCM)、“One Health in Asia Pacific” 2017年10月 口頭発表(一般) Kuching、Sarawak、Malaysia
  • Indoor exposure for polychlorinated biphenyls (PCBs) in domestic cats ~Pilot study of Mongolian pet cat~  [通常講演]
    Hazuki Mizukawa, Yoshinori Ikenaka、Tsend-ayush Sainnoxoi, Kohki Takaguchi, Kei Nomiyama, Shouta M.M. Nakayama, Mayumi Ishizuka
    The 2nd Symposium on the Improvement of Diagnosis and Prevention of some Diseases Caused by Environmental Change 2017年10月 口頭発表(一般) Ulaanbaatar、Mongolia
  • 全国規模での鳥類への鉛曝露:曝露指標として骨の利用の可能性  [通常講演]
    石井千尋, 中山翔太, 池中良徳, Kataba Andrew, 齊藤慶輔, 渡辺有希子, 槇納好岐, 松川岳久, 久保田章乃, 横山和仁, 水川葉月, 平田岳史, 石塚真由美
    第23回日本野生動物医学会大会 2017年09月 口頭発表(一般) 日本獣医生命科学大学、武蔵野市、東京都
  • エストロジェン類高感度分析法の開発と野生動物におけるスクリーニング  [通常講演]
    新屋 惣, 池中良徳, 中山翔太, 石井千尋, 水川葉月, 伊藤真輝, 高江洲昇, 大澤夏生, 綿貫 豊, 石塚 真由美
    第23回日本野生動物医学会大会 2017年09月 ポスター発表 日本獣医生命科学大学、武蔵野市、東京都 
    最優秀賞
  • 東京由来殺鼠剤抵抗性ラットを用いた新規抵抗性獲得機序の解明 ~ペントースリン酸経路の亢進による殺鼠剤代謝能の活性化~  [通常講演]
    武田一貴, 池中良徳, 田中和之, 谷川力, 中山翔太, 水川葉月, 石塚真由美
    第160回日本獣医学会学術年会 2017年09月 口頭発表(一般) 鹿児島大学、鹿児島市、鹿児島県
  • 鳥類におけるグルクロン酸抱合能の種差の解明と化学物質感受性の推定  [通常講演]
    新屋 惣, 池中良徳, 中山翔太, 水川葉月, 伊藤真輝, 高江洲昇, 齊藤 慶輔, 渡邊 有希子, 石塚 真由美
    日本鳥学会2017年度大会 2017年09月 ポスター発表 筑波大学、茨城県
  • 鳥類医学の発展に向けた新規腎障害マーカーの探索  [通常講演]
    石井千尋, 池中良徳, 川合佑典, 市居修, 中山翔太, 西村紳一郎, 大橋哲, 田中誠一, 齊藤慶輔, 渡邊有希子, 水川葉月, 石塚真由美
    日本鳥学会2017年度大会 2017年09月 ポスター発表 筑波大学、茨城県
  • 海鳥を介した汚染物質輸送  [通常講演]
    庄子晶子, K. Elliott, S. Aris-Brosou, 水川葉月, 中山翔太, 池中良徳, 石塚真由美, 桑江朝比呂, 渡辺謙太, 綿貫豊
    日本鳥学会2017年度大会 2017年09月 口頭発表(一般) つくば大学、つくば市、茨城県
  • Heavy metal residues in some ready-to-eat meat products marketed in Egypt  [通常講演]
    Wageh Sobhy Darwish, Yoshinori Ikenaka, Shouta M.M, Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    9th International Toxicology Symposium in Nigeria 2017年09月 口頭発表(一般) University of Benin、Benin、Nigeria
  • UDP-glucuronosyltransferase (UGT) 2B genetic feature and activities in Carnivores  [通常講演]
    Takamitsu Kondo, Yoshinori Ikenaka, Shouta M.M. Nakayama, Yusuke, K. Kawai, Hazuki Mizukawa, Yoko Mitani, Kei Nomyama, Shinsuke Tanabe, Mayumi Ishizuka
    9th International Toxicology Symposium in Nigeria 2017年09月 口頭発表(一般) University of Benin、Benin、Nigeria
  • The relationship of Blood Lead Levels between mothers and infants in Kabwe、 Zambia  [通常講演]
    Haruya Toyomaki, John Yabe, Shouta M.M. Nakayama, Yared, B. Yohannes, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Hokuto Nakata, Russell Dowling, Jack Caravanos, Mayumi Ishizuka
    9th International Toxicology Symposium in Nigeria 2017年09月 口頭発表(一般) University of Benin、Benin、Nigeria
  • Wild rat’s bio-distribution analysis for pesticide DDT from South Africa  [通常講演]
    Tetsuro Ogawa, Yoshinori Ikenaka, Yared B Yohannes, Lesa Thompson、Johan, van Vuren、Victor Wepener, Nico J Smit, Wynand Vlok、Shouta M.M, Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    9th International Toxicology Symposium in Nigeria 2017年09月 ポスター発表 University of Benin、Benin、Nigeria
  • Cats and dogs as sentinels for mercury exposure  [通常講演]
    Rafael BARROS, Hugo VILHENA, Hugo BRANCAL, Eg?dia GUERREIRO, Hazuki MIZUKAWA、Ana, C.A. SOUSA, M. Ramiro, PASTORINHO
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 ポスター発表 Hokkaido University、Sapporo、Japan
  • “First、 do no harm” ? balancing the benefits of malaria control against the toxic effects of DDT use  [通常講演]
    Lesa THOMPSON, Yoshinori IKENAKA, Yared YOHANNES、Atnafu ASSEFA, Johan VAN VUREN、Victor WEPENER, Nico SMIT, Wyna, d, VLOK、Wageh Sobhy, DARWISH, Shouta NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 口頭発表(一般) Hokkaido University、Sapporo、Japan
  • Drug metabolism enzymes in Carnivora species  [通常講演]
    Takamitsu Kondo, Yoshinori Ikenaka, Shouta M.M. Nakayama, Yusuke, K. Kawai, Hazuki Mizukawa, Yoko Mitani, Kei Nomyama, Shinsuke Tanabe, Mayumi Ishizuka
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 ポスター発表 Hokkaido University、Sapporo、Japan
  • Discovery of renal biomarker in birds by transcriptome analysis of kidney tissues and glycomics analysis of plasma following renal damage in chicken model  [通常講演]
    Chihiro Ishii, Yoshinori Ikenaka, Yusuke, K. Kawai, Osamu Ichii, Shouta. M.M, Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 ポスター発表 Hokkaido University、Sapporo、Japan
  • Blood Lead Levels in chickens and dogs around lead mining area、 Kabwe、 Zambia  [通常講演]
    Haruya Toyomaki, Shouta M.M. Nakayama, Yared, B. Yohannes, John Yabe, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 ポスター発表 Hokkaido University、Sapporo、Japan
  • Effects of Organohalogen Compounds on Serum Biochemical Profiles in Cats  [通常講演]
    Kraisiri Khidkhan, Hazuki Mizukawa, Kei Nomiyama, Yoshinori Ikenaka, Shota M.M, Nakayama, Nozomu Yokoyama, Kazuyoshi Sasaoka, Osamu Ichii, Mitsuyoshi Takiguchi, Hiroyuki Nishikawa, Keisuke Tanaka, Kohki Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 ポスター発表 Hokkaido University、Sapporo、Japan
  • Nobel investigation of mechanism of rodenticide resistance ~ enhanced pentose phosphate pathway causes rapid metabolism of warfarin ~  [通常講演]
    Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki, D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    The 5th Sapporo Summer Seminar for One Health (SaSSOH) 2017年09月 口頭発表(一般) Hokkaido University、Sapporo、Japan
  • Short-term or long-term in vivo exposure to organohalogen compounds in cats: Elucidate the metabolic capacities and effects on thyroid function  [通常講演]
    Hazuki Mizukawa, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kei Nomiyama, Misaki Maehara, Aksorn Saengtienchai, Tsend-ayush Sainnoxoi, Kraisiri Khidkhan, Nozomu Yokoyama, Kazuyoshi Sasaoka, Osamu Ichii, Mitsuyoshi Takiguchi, Khoki Takaguchi, Hiroki Nishikawa, Nana Takai, Shinsuke Tanabe, Mayumi Ishizuka
    9th International Toxicology Symposium in Nigeria 2017年09月 口頭発表(一般) University of Benin、 Benin、 Nigeria
  • Identification for the biotransformation of BDE209 by in vivo toxicokinetic analysis in Felis catus  [通常講演]
    Keisuke Tanaka, Kei Nomiyama, Hazuki Mizukawa, Kohki Takaguchi, Rumi Tanoue, Aksorn Saengtienchai、Tsend-ayush Sainnoxoi, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Shouta Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka, Tatsuya Kunisue, Shinsuke Tanabe
    37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 ポスター発表 Sheraton Wall Centre、Vancouver、Canada
  • Tissue Distribution of polychlorinated biphenyls and their metabolites in dog and cat  [通常講演]
    Kohki Takaguchi, Kei Nomiyama, Yasuko Nagano, Hazuki Mizukawa, Susumu Nakatsu, Tatsuya Kunisue, Shinsuke Tanabe
    37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 口頭発表(一般) Sheraton Wall Centre、Vancouver、Canada
  • DecaBDE might be associated with inhibition of thyroid hormone levels in serum of Japanese cats  [通常講演]
    Kei Nomiyama, Yasuo Yamamoto, Hazuki Mizukawa, Mitsuyoshi Takiguchi, Akifumi Eguchi, Shouta M. Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka, Tatsuya Kunisue, Shinsuke Tanabe
    37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 ポスター発表 Sheraton Wall Centre、Vancouver、Canada
  • in vivo exposure to PCBs in cats: Analysis of metabolic capacities and effects on the thyroid hormone homeostasis  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Hiroyuki Nishikawa, Misaki Maehara, Nana Takai, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kohki Takaguchi, Shinsuke Tanabe, Mayumi Ishizuka
    37th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2017) 2017年08月 口頭発表(一般) Sheraton Wall Centre、Vancouver、Canada
  • 陸棲哺乳類の血中に残留する臭素化フェノール類の曝露源解明と感受性の種差  [招待講演]
    水川葉月
    第44回日本毒性学会学術年会シンポジウム「ケミカルエコロジーと毒性学」 2017年07月 口頭発表(招待・特別) パシフィコ横浜、神奈川
  • 幼児のネオニコチノイド系農薬の曝露評価に関する研究  [通常講演]
    池中良徳, 宮原裕一, 一瀬貴大, 八木橋美緒, 中山翔太, 水川葉月, 平 久美子, 有薗幸司, 高橋圭介, 加藤恵介, 遠山千春, 石塚真由美
    第44回日本毒性学会学術年会 2017年07月 口頭発表(一般) パシフィコ横浜、横浜市、神奈川県
  • in vitro酵素活性試験による東京由来ワルファリン抵抗性クマネズミのワルファリン代謝能の解明  [通常講演]
    武田一貴, 池中良徳, 田中和之, 谷川 力, 中山翔太, 水川葉月, 石塚真由美, 肝灌流試験
    第44回日本毒性学会学術年会 2017年07月 口頭発表(一般) パシフィコ横浜、横浜市、神奈川県 
    優秀研究発表賞
  • Studies on polycyclic aromatic hydrocarbons formation in heat-treated meat  [通常講演]
    Wageh SD, Ikenaka Y, Nakayama SMM, Mizukawa H, Ishizuka M
    第44回日本毒性学会学術年会 2017年07月 口頭発表(一般) パシフィコ横浜、横浜市、神奈川県
  • ポリ塩化ビフェニル(PCBs)のin vivo暴露によるネコの異物代謝解明と甲状腺ホルモンへの影響評価  [通常講演]
    水川葉月, 前原美咲, 横山望, 市居修, 滝口満喜, 野見山桂, 西川博之, 池中良徳, 中山翔太, 高口倖暉, 田辺信介, 石塚真由美
    第44回日本毒性学会学術年会 2017年07月 ポスター発表 パシフィコ横浜、横浜市、神奈川県
  • Utility evaluation for stable Pb isotopes analysis using potential sentinel animals to identify Pb pollution sources  [通常講演]
    Hokuto Nakata, Shouta M.M, Nakayama、John Yabe、Allan Liazambi, Hazuki Mizukawa, Wageh Sobhy Darwish, Yoshinori Ikenaka, Mayumi Ishizuka
    International Conference on Geology, Mining, Mineral and Groundwater Resources of the Sub-Saharan Africa 2017年07月 口頭発表(一般) Livingstone、Zambia
  • In situ肝灌流試験によるワルファリン抵抗性クマネズミでのワルファリン代謝能の解明  [通常講演]
    武田一貴, 池中良徳, 田中和之, 谷川 力, 中山翔太, 水川葉月, 石塚真由美
    第26回環境化学討論会 2017年06月 ポスター発表 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • A Pilot Study on Exposure of Lead (Pb) and Its Blood Level Burden in Children from Kabwe、 Zambia: Implication to the General Population.  [通常講演]
    Yohannes YB, Nakayama SMM, Toyomaki H, Yabe J, Muzandu K, Kataba A, Ikenaka Y, Mizukawa H, Dowling R, Caravanos J, Ishizuka M
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • 幼児における尿中ネオニコチノイド濃度とその曝露実態の解明  [通常講演]
    池中良徳, 宮原裕一, 一瀬貴大, 八木橋美緒, 中山翔太, 水川葉月, 遠山千春, 石塚真由美
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • ヒト生体試料を用いたネオニコチノイドおよびネオニコチノイド代謝物の分析方法  [通常講演]
    一瀬貴大, 池中良徳, 藤岡一俊, 平 久美子, 中山翔太, 水川葉月, 八木橋美緒, 有薗幸司, 加藤恵介, 高橋圭介, 石塚真由美
    第26回環境化学討論会 2017年06月 ポスター発表 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • Blood Lead Levels in chickens and dogs around lead mining area、 Kabwe、 Zambia.  [通常講演]
    Toyomaki H, Nakayama SMM, Yohannes YB, Yabe J, Muzandu K, Mizukawa H, Ikenaka Y, Ishizuka M
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)の生体内変化  [通常講演]
    田中啓介, 野見山桂, 水川葉月, 口倖暉, 田上瑠美, Aksorn Saengtienchai、Tsend-ayush Sainnoxoi, 横山望, 市居修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • PCBs曝露がイヌ・ネコの甲状腺ホルモン恒常性へ及ぼす影響  [通常講演]
    髙口倖暉, 野見山桂, 西川博之, 水川葉月, 田上瑠美, 芳之内結加, 横山 望, 市居 修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 岩田久人, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • 血清中メタボローム解析によるイエネコのPCBs毒性影響評価  [通常講演]
    野見山桂, 西川博之, 水川葉月, 髙口倖暉, Tsend-ayush Sainnoxoi, 横山 望, 市居 修, 滝口満喜, 石塚真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • 高次栄養段階生物への化学物質の感受性の種差  [通常講演]
    石塚真由美, 中山翔太, 水川葉月, 池中良徳
    環境ホルモン学会2017年度第31回講演会 2017年06月 口頭発表(一般) 東京大学、東京都
  • ポリ塩化ビフェニル(PCBs)暴露によるネコ甲状腺機能への影響評価〜甲状腺ホルモンの脱ヨード化と代謝能の解析〜  [通常講演]
    水川葉月, 高居名菜, 横山望, 市居修, 滝口満喜, 野見山桂, 高口倖暉, 西川博之, 池中良徳, 中山翔太, Tsend-ayush Sainnoxoi, 田辺信介, 石塚真由美
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • イエネコのin vivo長期曝露試験:デカブロモジフェニルエーテル(BDE209)は甲状腺機能亢進症を引き起こすのか?  [通常講演]
    水川葉月, 野見山桂, 池中良徳, 中山翔太, Aksorn Saengtienchai、Tsend-ayush Sainnoxoi, 横山望, 笹岡一慶, 市居修, Kraisiri Khidkhan, 高居名菜, 滝口満喜, 田辺信介, 石塚真由美
    第26回環境化学討論会 2017年06月 口頭発表(一般) 静岡県コンベンションアーツセンター「グランシップ」、静岡市、静岡県
  • Inveatigation of biochemical changes in cat exposed organohalogen compounds  [通常講演]
    Kraisiri Khidkhan, Hazuki Mizukawa, Kei Nomiyama, Yoshinori Ikenaka, Shota M. M. Nakayama, Nozomu Yokoyama, Kazuyoshi Sasaoka, Osamu Ichii, Mitsuyoshi Takiguchi, Hiroyuki Nishikawa, Keisuke Tanaka, Kohki Takiguchi, Shinsuke Tanabe, Mayumi Ishizuka
    The 19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO 19) 2017年06月 ポスター発表 Matsuyama University、Matsuyama、Ehime
  • UDP-glucuronosyltransferase (UGT) among Pinnipedia and other carnivores  [通常講演]
    Takamitsu Kondo, Yoshinori Ikenaka, Shouta M.M. Nakayama, Yusuke, K. Kawai, Hazuki Mizukawa, Yoko Mitani, Kei Nomiyama, Shinsuke Tanabe, Mayumi Ishizuka
    The 19th International Symposium on Pollutant Responses in Marine Organisms (PRIMO 19) 2017年06月 口頭発表(一般) Matsuyama University、Matsuyama、Ehime
  • 代謝酵素の動物種差  [招待講演]
    石塚真由美, 中山翔太, 水川葉月, 池中良徳
    日本薬学会第137年会シンポジウム 2017年 口頭発表(招待・特別) 仙台国際センター、仙台市
  • In situ肝灌流モデルによる殺鼠剤抵抗性野生クマネズミにおけるワルファリン代謝能の解明  [通常講演]
    武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川 力, 水川葉月, 石塚真由美
    第39回日本分子生物学会年会 2016年11月 ポスター発表 パシフィコ横浜、神奈川
  • Cross omics analysis for PCBs toxicity in the dog brain -Effect of mitochondria function-.  [通常講演]
    Kohki Takaguchi, Kei Nomiyama、Lauan, Maria Claret, Momoko Kusaki, Jean Yoo, Hazuki Mizukawa, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
    The 7th SETAC World Congress/37th SETAC North America Annual Meeting 2016年11月 ポスター発表 Rosen Shingle Creek、Orlando、FL、USA
  • Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats and dogs: effects on thyroid hormones homeostasis.  [通常講演]
    Kei Nomiyama, Yasuo Yamamoto, Hazuki Mizukawa, Mitsuyoshi Takiguchi, Akifumi Eguchi, Shouta M.M. Nakayama, Yoshinori Ikenaka, Mayumi Ishizuka, Tatsuya. Kunisue, Shinsuke Tanabe
    The 7th SETAC World Congress/37th SETAC North America Annual Meeting 2016年11月 ポスター発表 Rosen Shingle Creek、Orlando、FL、USA
  • Monitoring of blood lead levels (BLLs) in dogs and chickens from lead pollution area, Kabwe, Zambia  [通常講演]
    Haruya Toyomaki, Shouta M.M. Nakayama, Yared, B. Yohannes, John Yabe, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    International Doctoral Symposium on Sustainable Management of Resources and Environment in the 21st Century 2016年11月 ポスター発表 Hokkaido University、Hokkaido、Japan
  • Characteristic for biotransformation of polychrolinated biphenyls in cats.  [通常講演]
    Hazuki Mizukawa, Misaki Maehara, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Kei Nomiyama, Hiroyuki Nishikawa, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kohki Takaguchi, Shinsuke Tanabe, Mayumi Ishizuka
    The 7th SETAC World Congress/37th SETAC North America Annual Meeting 2016年11月 ポスター発表 Rosen Shingle Creek、 Orlando、USA
  • Disruption of mitochondrial functions by PCB exposure in the dog brain: Toxicological assessment using cross omics.  [通常講演]
    Kohki Takaguchi, Kei Nomiyama, Akifumi Eguchi, Lauan, Maria Claret, Momoko Kusaki, Jean Yoo, Hazuki Mizukawa, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
    The 9th International PCB Workshop 2016年10月 ポスター発表 Kobe International Conference Center、Kobe、Japan
  • Toxicological assessment of PCBs and OH-PCBs in the brain of dogs using metabolomics approach.  [通常講演]
    Kei Nomiyama, Kohki Takaguchi, Akifumi Eguchi, Hazuki Mizukawa, Jean Yoo, Saya Tamura, Tomoko Oshihoi, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
    The 9th International PCB Workshop 2016年10月 口頭発表(一般) Kobe International Conference Center、Kobe、Japan
  • Genetic effects of the pesticide DDT on birds: lessons learned from a field study in South Africa  [通常講演]
    Lesa Thompson, Yoshinori Ikenaka, Yared Yohannes, Toshio Tsubota, Johan, van Vuren、Victor Wepener, Nico Smit, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    The 9th International Meeting of the Asian Society of Conservation Medicine (ASCM) “”One Health in Asia-Pacific”" 2016年10月 口頭発表(一般) Taipei Zoo、Taipei、Taiwan
  • In vivo analysis of PCB metabolic capacities and effects on the thyroid hormone in cats.  [通常講演]
    Hazuki Mizukawa, Misaki Maehara, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Kei Nomiyama, Hiroyuki Nishikawa, Kohki Takaguchi, Aksorn Saengtienchai, Tsend-ayush Sainnoxoi, Yoshinori Ikenaka, Shouta M.M. Nakayama, Shinsuke Tanabe, Mayumi Ishizuka
    The 9th International PCB Workshop 2016年10月 口頭発表(一般) Kobe International Conference Center、 Kobe、 Japan
  • 犬と猫の慢性腎臓病における尿細管周囲毛細血管の変化  [通常講演]
    中村麗菜, 矢吹 映, 市居 修, 水川葉月, 横山 望, 大和 修
    第159回日本獣医学会学術集会 2016年09月 口頭発表(一般) 日本大学生物資源科学部、藤沢市、神奈川県
  • 家畜・家禽の鉛安定同位体生体内分別の種差および歩哨動物としての有用性評価  [通常講演]
    栂尾正雄, 中山翔太, John Yabe, 池中良徳, 中田北斗, 石井千尋, 水川葉月, Yared B. Yohannes, Aksorn Saengtienchai, 石塚真由美
    第159回日本獣医学会学術集会 2016年09月 口頭発表(一般) 日本大学生物資源科学部、藤沢市、神奈川県
  • 野生動物及び実験動物におけるグルクロン酸抱合酵素の動物種差解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, Aksorn Saengtienchai, Yared Beyene, 坂本健太郎, 和田昭彦, 服部 薫, 三谷曜子, 田辺信介, 野見山 桂, 石塚真由美
    第159回日本獣医学会学術集会 2016年09月 口頭発表(一般) 日本大学生物資源科学部、藤沢市、神奈川県
  • 鳥類におけるグルクロン酸抱合酵素による薬物代謝  [通常講演]
    新屋 惣, 池中良徳, 中山翔太, 石井千尋, 水川葉月, 齊藤慶輔, 渡邉有希子, 伊藤真輝, 高江洲昇, 坂本健太郎, 石塚真由美
    第22回日本野生動物医学会 2016年09月 口頭発表(一般) 宮崎市民プラザ、宮崎
  • 鳥類における新規腎障害マーカーの探索  [通常講演]
    石井千尋, 池中良徳, 市居修, 中山翔太, 西村紳一郎, 大橋 哲, 田中誠一, 齊藤慶輔, 渡邊有希子, 水川葉月, 石塚真由美
    第22回日本野生動物医学会 2016年09月 ポスター発表 宮崎市民プラザ、宮崎
  • UGT2Bサブファミリーに着目した食肉目におけるグルクロン酸抱合能の動物種差解明  [通常講演]
    近藤誉充, 池中良徳, 中山翔太, 水川葉月, Aksorn Saengtienchai, Yared Beyene, 坂本健太郎, 和田昭彦, 服部薫, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美
    平成28年度内外環境応答・代謝酵素研究会 2016年09月 口頭発表(一般) 静岡県立大学薬学部、静岡市、静岡県
  • 野生げっ歯類の殺鼠剤の抵抗性メカニズムと非対象動物に対する毒性メカニズム  [通常講演]
    石塚真由美, 中山翔太, 水川葉月, 池中良徳
    日本哺乳類学会2016年度大会「企画シンポジウム:野生動物が関わる問題にどのように対応するか?〜基礎研究を応用した解決への取り組み〜」 2016年09月 口頭発表(一般) 筑波大学、つくば市、茨城県
  • Playing “”chicken”" ? a xenobiotic dilemma  [通常講演]
    Lesa THOMPSON, Yoshinori IKENAKA, Yared YOHANNES、Atnafu ASSEFA, Johan VAN VUREN、Victor WEPENER, Nico SMIT, Wynand VLOK, Shouta NAKAYAMA, Hazuki MIZUKAWA, Mayumi ISHIZUKA
    The 4th Sapporo Summer Seminar for One Health (SaSSOH) 2016年09月 ポスター発表 Sapporo、Japan
  • The effect of environmental pollutants to pet animals ~Level and effects of Persistent organic pollutants (POPs) to domestic cat~.  [通常講演]
    Tsend-ayush Sainnokhoi, Hazuki Mizukawa, Misaki Maehara, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Kei Nomiyama, Aksorn Saengtienchai, Shinsuke Tanabe, Yoshinori Ikenaka, Shouta M.M. Nakayama, Mayumi Ishizuka
    The 4th Sapporo Summer Seminar for One Health (SaSSOH) 2016年09月 ポスター発表 Sapporo、Japan
  • A glycomics approach to renal biomarkers in birds.  [通常講演]
    Chihiro Ishii, Yoshinori Ikenaka, Osamu Ichii, Shouta M.M. Nakayama, Hazuki Mizukawa, Shin-Ichiro Nishimura, Tetsu Ohashi, Masakazu Tanaka, Keisuke Saito, Yukiko Watanabe, Mayumi Ishizuka
    The 4th Sapporo Summer Seminar for One Health (SaSSOH) 2016年09月 ポスター発表 Sapporo、Japan
  • Lead exposure on human samples in African countries and lead pollution in Kabwe, Zambia.  [通常講演]
    Haruya Toyomaki, Shouta M.M. Nakayama, Yared, B. Yohannes, John Yabe, Kaampwe Muzandu, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    The 4th Sapporo Summer Seminar for One Health (SaSSOH) 2016年09月 ポスター発表 Sapporo、Japan
  • Investigation of Warfarin Metabolic Ability of Warfarin Resistant Roof Rats by in situ Liver Perfusion  [通常講演]
    Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki, D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    The 4th Sapporo Summer Seminar for One Health (SaSSOH) 2016年09月 ポスター発表 Sapporo、Japan
  • Pharmacokinetic analysis of warfarin resistant roof rats (Rattus rattus) in Tokyo  [通常講演]
    Kazuki Takeda, Yoshinori Ikenaka, Tsutomu Tanikawa, Kazuyuki, D. Tanaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 ポスター発表 Giza、Egypt
  • Is there species difference of biological variation of lead (Pb) isotopic composition between livestock and poultry?  [通常講演]
    Masao Togao, Shouta M.M, Nakayama、John Yabe, Hokuto Nakata, Yoshinori Ikenaka, Chihiro Ishii, Yared B. Yohannes, Hazuki Mizukawa, Wageh Sobhy Darwish、Aksorn Saengtienchai, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 ポスター発表 Giza、Egypt
  • Pb levels and isotope ratio profiles in kidneys of Japanese wild rats  [通常講演]
    Shouta M.M, Nakayama, Hokuto Nakata, Balazs Oroszlany, Yoshinori Ikenaka, Hazuki Mizukawa, Kazuyuki Tanaka, Tsunehito Harunari, Tsutomu Tanikawa, Yared, B. Yohannes, Aksorn Saengtienchai, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 ポスター発表 Giza、Egypt
  • Cytochrome P450 expression in the rabbit、8th International Toxicology Symposium in Africa  [通常講演]
    Walaa Fathy Saad, Eldin、Wageh, S. Darwish, Yoshinori Ikenaka, Shouta M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 ポスター発表 Giza、Egypt
  • Creating a model of renal damage in chicken and glycomic approach to identify novel biomarkers for kidney injury in birds  [通常講演]
    Chihiro Ishii, Yoshinori Ikenaka, Osamu Ichii, Shouta M.M. Nakayama, Shin-Ichiro Nishimura, Tetsu Ohashi, Masakazu Tanaka, Keisuke Saito, Yukiko Watanabe, Hazuki Mizukawa, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 口頭発表(一般) Giza、Egypt
  • Lead exposure on human samples in African countries: a mini review  [通常講演]
    Haruya Toyomaki, Shouta M.M. Nakayama, Yared, B. Yohannes, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 口頭発表(一般) Giza、Egypt
  • Metabolic capacities of polychlorinated biphenyls (PCBs) in cats and dogs  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Shinsuke Tanabe, Misaki Maehara, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama, Mayumi Ishizuka
    8th International Toxicology Symposium in Africa 2016年08月 ポスター発表 Giza、 Egypt
  • トビケラウオッチ(第2報)水生昆虫を用いた河川環境の放射性セシウムモニタリング  [通常講演]
    上野大介, 大坪栄二郎, 染谷孝, 水川葉月, 稲波修, 長坂洋光, 藤野毅, 渡邉泉, 大葉隆, 龍田希, 仲井邦彦
    第5回環境放射能除染研究発表会 2016年07月 ポスター発表 とうほう・みんなの文化センター(福島県文化センター)、福島
  • クロスオミックスによるイヌ脳の PCBs 毒性評価(Cross omics analysis for PCBs toxicity in the dog brain)  [通常講演]
    髙口倖暉, 野見山桂, Lauan Maria Claret, Yoo Jean, 水川葉月, 岩田久人, 国末達也, 田辺信介
    日本プロテオーム学会2016年大会 2016年07月 ポスター発表 北里大学、東京
  • Human health risk due to polycyclic aromatic hydrocarbon (PAHs) exposure in Kumasi、 Ghana; and excretion of PAH metabolites in cattle urine from urban and rural sites.  [通常講演]
    Bortey-Sam N, Ikenaka Y, Nakayama SMM, Saengtienchai A, Mizukawa H, Ishizuka M
    第30回北海道薬物作用談話会 2016年07月 口頭発表(一般) 北海道大学、札幌
  • KAMPAI -Team 2、PAST、PRESENT and FUTURE-  [通常講演]
    Shouta M.M, Nakayama、John Yabe, Yared Beyene, Haruya Toyomaki, Kaampwe Muzandu, Kennedy Choongo, Hazuki Mizukawa, Yoshinori Ikenaka, Mayumi Ishizuka
    Kick-Off Symposium for the “The Project for Visualization of impact of chronic/latent chemical hazard and Geo-Ecological Remediation in Zambia” 2016年07月 口頭発表(一般) CRESTA Golfview Hotel、Lusaka、Zambia
  • N-glycans as potential?novel biomarkers for kidney injury in avian species.  [通常講演]
    Chihiro Ishii, Yoshinori Ikenaka, Osamu Ichii, Shouta M.M. Nakayama, Hazuki Mizukawa, Shin-Ichiro Nishimura, Tetsu Ohashi, Masakazu Tanaka, Keisuke Saito, Yukiko Watanabe, Mayumi Ishizuka
    65th International?Conference of the Wildlife Disease Association 2016年07月 ポスター発表 Greek Peak Mountain Resort、Cortland、New York、USA
  • トビケラウォッチ 水生昆虫をもちいた河川の環境放射能モニタリング(第 3 報)  [通常講演]
    大坪栄二郎, 上野大介, 染谷 孝, 水川葉月, 稲波 修, 長坂洋光, 藤野 毅, 渡邉 泉, 大葉 隆, 龍田 希, 仲井邦彦
    第25回環境化学討論会 2016年06月 ポスター発表 朱鷺メッセ、新潟
  • メタボロミクスアプローチを用いたイヌ脳へのOH-PCBs毒性機序の解明  [通常講演]
    野見山 桂, 高口倖暉, 江口哲史, 水川葉月, Yoo Jean, 田村沙弥, 忍穂井智子, 岩田久人, 国末達也, 田辺信介
    第25回環境化学討論会 2016年06月 口頭発表(一般) 朱鷺メッセ、新潟
  • クロスオミックスによるイヌ脳へのPCBs毒性評価:ミトコンドリア機能へ及ぼす影響解析  [通常講演]
    高口倖暉, 野見山 桂, Lauan Maria Claret, 草木桃子, Yoo Jean, 水川葉月, 岩田久人, 国末達也, 田辺信介
    第25回環境化学討論会 2016年06月 口頭発表(一般) 朱鷺メッセ、新潟
  • 有機ハロゲン化合物によるコンパニオンアニマルの汚染実態と甲状腺ホルモンへの影響評価  [通常講演]
    野見山 桂, 山本恭央, 水川葉月, 江口哲史, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介
    第25回環境化学討論会 2016年06月 口頭発表(一般) 朱鷺メッセ、新潟
  • ヒト尿中のネオニコチノイドおよびその代謝産物の分析法の確立とヒトへの曝露評価  [通常講演]
    池中良徳, 藤岡一俊, 平 久美子, 中山翔太, 水川葉月, 一瀬貴大, Jemima Tiwaa Marfo, 有薗幸司, 加藤恵介, 石坂真澄, 馬場浩司, 川上智規, 石塚真由美
    第25回環境化学討論会 2016年06月 口頭発表(一般) 朱鷺メッセ、新潟
  • メタボローム解析を用いたPCBsによるイエネコの毒性影響評価  [通常講演]
    西川博之, 野見山桂, 水川葉月, 横山望, 市居修, 滝口満喜, 石塚真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介
    第25回環境化学討論会 2016年06月 口頭発表(一般) 朱鷺メッセ、新潟
  • UGTの動物種差  [通常講演]
    石塚真由美, 中山翔太, 水川葉月, 池中良徳
    第43回日本毒性学会学術年会 2016年06月 口頭発表(一般) ウインクあいち、愛知
  • ハリネズミは化学物質高感受性種? African hedgehog (Atalerix albiventris)で観察された低薬物代謝能  [通常講演]
    アクソルン サエンティエンチャイ, 池中良徳, ネスタ サム, ウスマ ジャムナーク, 水川葉月, 中山翔太, 石塚真由美
    第43回日本毒性学会学術年会 2016年06月 ポスター発表 ウインクあいち、愛知
  • 東京由来ワルファリン抵抗性クマネズミクローズドコロニーでのワルファリン体内動態の解明  [通常講演]
    武田一貴, 池中良徳, 田中和之, 谷川力, 中山翔太, 水川葉月, 石塚真由美
    第43回日本毒性学会学術年会 2016年06月 口頭発表(一般) ウインクあいち、愛知
  • 脂肪組織における芳香族炭化水素受容体(AhR)の機能解明  [通常講演]
    本平航大, 池中良徳, ワギ ダルウィシュ, 中山翔太, 水川葉月, 石塚真由美
    第43回日本毒性学会学術年会 2016年06月 口頭発表(一般) ウインクあいち、愛知
  • ネコにおけるポリ塩化ビフェニル(PCBs)の異物代謝機構解明と甲状腺ホルモンへの影響評価  [通常講演]
    水川葉月, 前原美咲, 横山望, 市居修, 滝口満喜, 野見山桂, 西川博之, 池中良徳, 中山翔太, 高口倖暉, 田辺信介, 石塚真由美
    第25回環境化学討論会 2016年06月 口頭発表(一般) 朱鷺メッセ、新潟
  • ポリ塩化ビフェニル(PCBs)のin vivo暴露によるネコの異物代謝解明と甲状腺ホルモンへの影響評価  [通常講演]
    水川葉月, 前原 美咲, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 西川 博之, 池中 良徳, 中山 翔太, 高口 倖暉, 田辺 信介, 石塚 真由美
    第43回日本毒性学会学術年会 2016年06月 口頭発表(一般) ウインクあいち、愛知
  • Plasma essential trace element concentrations in dogs with chronic enteropathy  [通常講演]
    Nozomu Yokoyama, Hiroshi Ohta, Hazuki Mizukawa, Yumiko Kagawa, Shouta Nakayama, Noboru Sasaki, Keitaro Morishita, Kensuke Nakamura, Yoshinori Ikenaka, Mayumi Ishizuka, Mitsuyoshi Takiguchi
    American College of Veterinary Internal Medicine Forum 2016 2016年06月 口頭発表(一般) Colorado convention center、Colorado、U.S.A
  • Contamination of eggs from free-ranging chickens in a malaria-endemic region using DDT as vector control  [通常講演]
    Lesa Thompson, Yoshinori Ikenaka, Yared Yohannes、Johan van Vuren、Victor Wepener, Nico Smit、Wynand Vlok, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    International Society for Environmental Epidemiology-International Society of Exposure Sciences Asia Chapter (ISEE-ISES AC) 2016 2016年06月 口頭発表(一般) Hokkaido University、Sapporo、Japan
  • Human health risk due to polycyclic aromatic hydrocarbon (PAHs) exposure in Kumasi, Ghana and excretion of PAH metabolites in cattle urine from urban and rural sites  [通常講演]
    Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M.M. Nakayama, Kwadwo, A. Asante, Christian Obirikorang, Elvis Baidoo, Aksorn Saengtienchai, Hazuki Mizukawa, Mayumi Ishizuka
    SETAC Europe 26th Annual Meeting 2016年05月 ポスター発表 La Cite Nantes Congress Center、Nantes、France
  • Risk versus benefits of controlling vector-borne diseases using insecticides: a case example in South Africa  [通常講演]
    Lesa Thompson, Yoshinori Ikenaka, Yared Yohannes、Johan van Vuren、Victor Wepener, Nico Smit, Wynand Vlok, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    2nd International Conference on One Medicine One Science (iCOMOS) 2016年04月 ポスター発表 University of Minnesota、Minneapolis、Minnesota、USA
  • 非実験動物における化学物質代謝能の特徴と種差  [招待講演]
    池中良徳, 筧麻友, 水川葉月, 中山翔太, 石塚真由美
    日本薬学会第136年会(横浜) 2016年03月 口頭発表(一般) パシフィコ横浜、神奈川
  • CONTAMINATION STATUS OF ORGANOHALOGEN COMPOUNDS AND THEIR HYDROXYLATED METABOLITES IN PET CATS AND DOGS: EFFECTS ON THYROID HORMONES HOMEOSTASIS  [通常講演]
    Yasuo Yamamoto, Kei Nomiyama, Hazuki Mizukawa, Mitsuyoshi Takiguchi, Mayumi Ishizuka, Tatsuya Kunisue, Sinsuke Tanabe
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • CADDISFLY WATCH ? A MONITORING APPROACH FOR RADIOACTIVE CESIUM IN RIVER ENVIRONMENT OF FUKUSHIMA USING CADDISFLY LARVAE  [通常講演]
    Daisuke Ueno, Hazuki Mizukawa, Osamu Inanami, Takeshi Fujino, Takashi Ohba, Izumi Watanabe, Chiharu Tohyama, Hiromitsu Nagasaka, Takashi Someya, Nozomi Tatsuta, Kunihiko Nakai
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • HEPATIC TRANSCRIPTOME ANALYSIS TO ASSESS THE EFFECTS OF POLYCHLORINATED BIPHENYLS ON BEAGLE DOGS  [通常講演]
    Saya Tamura, Jean Yoo, Hazuki Mizukawa, Tetsuro Agusa, Masashi Hirano, Akifumi Eguchi, Kei Nomiyama, Shinsuke Tanabe, Eun-Young Kim, Hisato Iwata
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • PROTEOMIC ANALYSIS FOR PCBs TOXICITY IN THE DOG BRAIN  [通常講演]
    Kohki Takaguchi, Kei Nomiyama、Lauan, Maria Claret, Momoko Kusaki, Jean Yoo, Hazui Mizukawa, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • TOXICOLOGICAL ASSESSMENT OF PCBS AND HYDROXY-METABOLITES (OH-PCBs) IN THE BRAIN OF DOGS USING METABOLOMICS APPROACH  [通常講演]
    Kei Nomiyama, Akifumi Eguchi, Hazuki Mizukawa, Jean Yoo, Kohki Takaguchi, Yasuo Yamamoto, Tomoko Oshihoi, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • TOXICOLOGICAL ASSESSMENT OF PCBS AND OH-PCBS IN CAT USING METABOLOMICS APPROACH  [通常講演]
    Hiroyuki Nishikawa, Kei Nomiyama, Akifumi Eguchi, Hazuki Mizukawa, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M, M. Nakayama, Shinsuke Tanabe
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • BIOTRANSFORMATION POTENCIES OF POLYCHLORINATED BIPHENYLS IN CATS  [通常講演]
    Hazuki Mizukawa, Misaki Maehara, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Kei Nomiyama, Yoshinori Ikenaka, Shouta M.M.Nakayama, Shinsuke Tanabe, Mayumi Ishizuka
    International Symposium on Environmental Chemistry and Toxicology ? To Accelerate a Global Network of Environmental Researchers 2016年03月 ポスター発表 Ehime University, Ehime, Japan
  • 犬の慢性腸症における血中微量元素プロファイルの解析  [通常講演]
    横山 望, 大田 寛, 水川葉月, 賀川由美子, 中山翔太, 佐々木 東, 森下啓太郎, 中村健介, 池中良徳, 石塚真由美, 滝口満喜
    第12回日本内科学アカデミー学術集会 2016年02月 口頭発表(一般) パシフィコ横浜、神奈川
  • 化学物質の汚染環境に生きる野生動物の適応戦略  [招待講演]
    石塚真由美, 中山翔太, 水川葉月, 池中良徳
    一般社団法人日本環境化学会25周年記念講演会:「環境化学の今、そして未来へ−環境化学からの提言−」 2016年 口頭発表(招待・特別) 発明会館地下ホール、東京都
  • ペット動物におけるPCB汚染実態と代謝能解析  [通常講演]
    水川葉月
    第6回PCB講演会(札幌) 2015年12月 口頭発表(一般) 北海道大学、北海道
  • Tracing migration of northern fur seals in Russian-Japanese waters using stable isotopes in whiskers  [通常講演]
    Ayako Yamada, Takanori Horimoto, Yoshinori Ikenaka, Shouta M, M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Yoko Mitani, Kazushi Miyashita
    21st Biennial Conference on Marine Mammals 2015年12月 ポスター発表 Hilton San Francisco Union Square、San Francisco、USA
  • Diet records in winter, migration and diving behavior during early summer of juvenile Steller sea lions in the asian stock  [通常講演]
    Keizo Ito、Nao Takahashi, Kaoru Hattori, Yoko Goto, Takuya Nakanowatari, Kay, I. Ohshima, Takanori Horimoto, Yoshinori Ikenaka, Shouta M, M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Kazushi Miyashita, Yoko Mitani
    21st Biennial Conference on Marine Mammals 2015年12月 ポスター発表 Hilton San Francisco Union Square、San Francisco、USA
  • The habitat use of northern fur seals Callorhinus ursinus around northern Japan during non-breeding season, defined from stable isotopes and trace elements in whiskers  [通常講演]
    Takanori Horimoto, Svetlana Artemyeva, Peter Permyakov, Mari Kobayashi, Kaoru Hattori, Hiromi Kasai, Hiroshige Tanaka, Yoshinori Ikenaka, Shouta M, M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Vladimir Burkanov, Russel Andrews, Yoko Mitani, Yasunori Sakurai
    21st Biennial Conference on Marine Mammals 2015年12月 ポスター発表 Hilton San Francisco Union Square、San Francisco、USA
  • Impact of anthropogenic and naturally occurring brominated compounds in veterinary field  [通常講演]
    Hazuki Mizukawa, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama, Kei Nomiyama, Misaki Maehara, Shinsuke Tanabe, Mayumi Ishizuka
    Leading Symposium on Chemical Hazard and Adaptability in Animals The Current Challenge of Young Researchers 2015年12月 口頭発表(一般) Hokkaido University、Hokkaido、Japan
  • Contamination status of organohalogen compounds and their hydroxylated metabolites in pet cats: effects on thyroid hormones homeostasis  [通常講演]
    Yasuo Yamamoto, Kei Nomiyama, Hazuki Mizukawa, Mitsuyoshi Takiguchi, Mayumi Ishizuka, Tatsuya Kunisue, Shinsuke Tanabe
    SETAC North America 36th Annual Meeting 2015年11月 ポスター発表 Salt Palace Convention Center、Salt Lake City、USA
  • Omics approach of proteomic and metabolomic analysis for PCBs toxicity in the dog brain  [通常講演]
    Kohki Takaguchi, Kei Nomiyama、Lauan, M. Claret, Momoko Kusaki, Jean Yoo, Hazuki Mizukawa, Hisato Iwata, Tatsuya Kunisue, Shinsuke Tanabe
    SETAC North America 36th Annual Meeting 2015年11月 ポスター発表 Salt Palace Convention Center、Salt Lake City、USA
  • Metabolism and biotransformation of organohalogen compounds in the liver microsomes of cats and dogs  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Nozomu Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama, Misaki Maehara, Shinsuke Tanabe, Mayumi Ishizuka
    SETAC North America 36th Annual Meeting 2015年11月 ポスター発表 Salt Palace Convention Center、Salt Lake City、USA
  • Phase II xenobiotic metabolizm in carnivora -UGT activity and genomestructure in Pinniped species-  [通常講演]
    Yoshinori Ikenaka, Mayu Kakehi, Shouta Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    7th SETAC Africa Conference 2015年10月 口頭発表(一般) Langebaan、South Africa
  • 抗血液凝固系殺鼠剤の標的分子であるビタミンKエポキシド還元酵素(VKOR)の鳥類における特徴及び種差  [通常講演]
    森田鮎子, 池中良徳, 中山翔太, 石井千尋, 水川葉月, 渡邉研右, 齊藤慶輔, 渡邊有希子, 伊藤真輝, 高江洲昇, 石塚真由美
    第158回日本獣医学会学術集会 2015年09月 口頭発表(一般) 北里大学獣医学部十和田キャンパス、青森
  • Exposure levels of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in wild rats in Kumasi, Ghana  [通常講演]
    Nesta Bortey-Sam, Yoshinori Ikenaka, Osei Akoto, Shouta M.M. Nakayama, Yared, Beyene Yohannes, Elvis Baidoo, Aksorn Saengtienchai, Hazuki Mizukawa, Mayumi Ishizuka
    7th International Toxicology Symposium in Africa 2015年08月 口頭発表(一般) Johannesburg、South Africa
  • Assessment of exposure to toxic elements (As, Pb, Cd and Hg), and DDTs in four species of birds from Ethiopia  [通常講演]
    Yared Beyene Yohannes, Yoshinori Ikenaka, Shouta MM. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    7th International Toxicology Symposium in Africa 2015年08月 口頭発表(一般) Johannesburg、South Africa
  • HCH’s in two fish species from a large floodplain pan within a subtropical conservation area  [通常講演]
    Clare Edwards, Yoshinori Ikenaka, Yared Beyene, Shouta MM. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Victor Wepener、Johan, H.J. van Vuren
    7th International Toxicology Symposium in Africa 2015年08月 口頭発表(一般) Johannesburg、South Africa
  • Metallothionein as a biomarker for chicken exposure to heavy metals  [通常講演]
    Wageh S. Darwish, Walaa F, Saad Eldin, Nesta Bortey-Sam, Yoshinori Ikenaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    7th International Toxicology Symposium in Africa 2015年08月 口頭発表(一般) Johannesburg、South Africa
  • Lead pollution in the children in Kabwe mining area、 Republic of Zambia- Source identification by stable isotope analysis  [通常講演]
    Shouta M M, Nakayama、John Yabe, Yoshinori Ikenaka, Yared Beyene Yohannes, Balazs Oroszlany, Nesta Bortey-Sam, Kaampwe Muzandu, Hazuki Mizukawa, Kenedy Choongo、Abel Kabalo、Aaron Mweene, Mayumi Ishizuka
    7th International Toxicology Symposium in Africa 2015年08月 口頭発表(一般) Johannesburg、South Africa
  • Vector-control pesticide DDT detected in free-ranging chickens from South Africa  [通常講演]
    Lesa A Thompson, Yoshinori Ikenaka, Yared B Yohannes, Johan van Vuren、Victor Wepener, Nico J Smit, Wynand Vlok, Shouta M M, Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    7th International Toxicology Symposium in Africa 2015年08月 口頭発表(一般) Johannesburg、South Africa
  • Caddisfly Watch ? An monitoring approach for radioactive cesium in river environment of Fukushima using the caddisfly larvae  [通常講演]
    Daisuke Ueno, Hazuki Mizukawa, Osamu Inanami, Takeshi. Fujino, Takashi Ohoba, Izumi Watanabe, Chiharu Tohyama, Hiromitsu Nagasaka, Nozomi Tatsuta, Kunihiko Nakai
    13th International Conference on the Biogeochemistry of Trace Elements、ICOBTE 2015 FUKUOKA 2015年07月 ポスター発表 Fukuoka International Congress Center、Fukuoka、Japan
  • Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species  [通常講演]
    Lesa A Thompson, Yoshinori Ikenaka, Johannes, J, van Vuren、Victor Wepener, Nico Smit、Wynand Vlok、Yared B Yohannes, Hazuki Mizukawa, Shouta M, M, Nakayama, Mayumi Ishizuka
    Vth International Wildlife Management Congress 2015 2015年07月 ポスター発表 Sapporo、Japan
  • Lead poisoning and source identification in raptors from Japan  [通常講演]
    Chihiro Ishii, Shouta M.M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yared, B. Yohannes, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Yasushi Masuda, Kentaro, Q. Sakamoto, Mayumi Ishizuka
    Vth International Wildlife Management Congress 2015 2015年07月 口頭発表(一般) Sapporo、Japan
  • 阿武隈川および阿賀川水系における淡水魚137Csの生態学的半減期の推定  [通常講演]
    仲井邦彦, 上野大介, 水川葉月, 藤野 毅, 川田 暁, 泉 茂彦, 渡遺 泉, 長坂洋光, 松村 徹, 龍田 希, 稲波 修
    第24回環境化学討論会 2015年06月 ポスター発表 札幌コンベンションセンター、北海道
  • ペットネコにおける有機ハロゲン化合物および水酸化代謝物の汚染実態と甲状腺ホルモン恒常性への影響  [通常講演]
    山本恭央, 野見山桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺信介
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • ポリ塩化ビフェニル(PCBs)曝露によるイヌ異物代謝酵素シトクロムP450発現への影響評価  [通常講演]
    田村沙弥, 劉 珍, 水川葉月, 阿草哲郎, 平野将司, 野見山 桂, 田辺信介, 金 恩英, 岩田久人
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • ポリ塩化ビフェニル(PCBs)を曝露したイヌの脳プロテオーム解析による毒性影響評価  [通常講演]
    高口倖暉, 野見山桂, Lauan Maria Claret, 草木桃子, Yoo Jean, 水川葉月, 岩田久人, 国末達也, 田辺信介
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • メタボローム解析によるイヌ脳中PCBs、 OH-PCBsの毒性影響評価、第24回環境化学討論会  [通常講演]
    野見山 桂, 江口哲史, 水川葉月, Yoo Jean, 高口倖暉, 山本泰央, 忍穂井 智子, 岩田久人, 国末達也, 田辺信介
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • Vector-controlling pesticides in free-ranging chickens in KwaZulu-Natal、 South Africa  [通常講演]
    Thompson LA、Ikenaka Y, an Vuren JJ, Wepener V, Smit N, Vlok W, Yohannes YB, Mizukawa H, Nakayama SMM, Ishizuka M
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • Concentrations of polycyclic aromatic hydrocarbon metabolites (OH-PAHs) in domestic animal in Kumasi、 Ghana  [通常講演]
    Bortey-Sam N, Ikenaka Y, Akoto O, Nakayama SMM, Marfo J, Mizukawa H, Ishizuka M
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • Nitrate content in Egyptian meat products and its modulatory effects on xenobiotic metabolizing enzymes  [通常講演]
    Wageh SD, Mohamed AH, Ikenaka Y, Nakayama SMM, Mizukawa H, Ishizuka M
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • 猛禽類における鉛中毒問題と骨における鉛分布の解明  [通常講演]
    石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 森田鮎子, 齊藤慶輔, 渡邊有希子, 神 和夫, 田辺信介, 野見山 桂, 林 光武, 増田 泰, 坂本健太郎, 石塚真由美
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • 野生齧歯類におけるワルファリン抵抗性獲得機序の解明  [通常講演]
    武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川 力, 水川葉月, 石塚真由美
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • 第 II 相抱合反応の種差:食肉目におけるグルクロン酸抱合酵素の進化と遺伝子構造  [通常講演]
    池中良徳, 筧 麻友, 中山翔太, 川合佑典, 渡邊研右, 水川葉月, 野見山 桂, 田辺信介, 石塚真由美
    第24回環境化学討論会 2015年06月 口頭発表(一般) 札幌コンベンションセンター、北海道
  • ウマにおけるシトクロムP450 の特性評価:ジアゼパム代謝を担うCYP3Aの重要性  [通常講演]
    水川葉月, 中山翔太, 池中良徳, 速水 秋, Wageh Sobhy Darwish, 渡邊研右, 川合佑典, 石塚真由美
    第24回環境化学討論会 2015年06月 ポスター発表 札幌コンベンションセンター、北海道
  • Lead (Pb) poisoning in children from townships around an extensive lead-zinc mine in Kabwe、 the Republic of Zambia  [通常講演]
    Nakayama SMM, Yabe J, Ikenaka Y, Beyene YB, Bortey-Sam N, Oroszlany B, Muzandu K, Choongo K, Mweene A, Kabalo A, Mizukawa H, Ishizuka M
    日本地球惑星科学連合2015年大会 2015年05月 口頭発表(一般) 幕張メッセ、千葉
  • トビケラウオッチによる環境放射能モニタリング(第2報)?ヒゲナガカワトビゲラを用いた広域調査  [通常講演]
    上野大介, 龍田 希, 水川葉月, 藤野 毅, 大葉 隆, 渡邉 泉, 遠山千春, 鍋師裕美, 長坂洋光, 仲井邦彦
    第85回日本衛生学会学術総会 2015年03月 ポスター発表 和歌山県民文化会館・ホテルアバローム紀の国、和歌山
  • 日本海北部に来遊するキタオットセイの安定同位体比と利用海域との関係  [通常講演]
    山田綾子, 堀本高矩, 石塚真由美, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 宮下和士
    平成27年度日本水産学会春季大会 2015年03月 口頭発表(一般) 東京海洋大学品川キャンパス、東京
  • 外来化学物質の代謝の種差  [招待講演]
    石塚真由美, 中山翔太, 水川葉月, 池中良徳
    第31回日本毒性病理学シンポジウム「毒性病理学と獣医病理学を繋ぐ比較の生物学−共通性と種差を知ろう」 2015年 口頭発表(招待・特別) タワーホール船堀、東京
  • シンポジウム「野生鳥類の化学物質汚染とその影響」鳥類で起こっているケミカルハザードとそのメカニズム  [招待講演]
    中山翔太, 水川葉月, 池中良徳, 石塚真由美
    第21回日本野生動物医学会大会 2015年 口頭発表(招待・特別) 酪農学園大学、北海道
  • 食肉目におけるグルクロン酸抱合酵素の遺伝子解析  [通常講演]
    筧 麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部 薫, 田辺信介, 野見山 桂, 石塚真由美
    第37回日本分子生物学会年会 2014年11月 ポスター発表 横浜パシフィコ、神奈川
  • Distribution of toxic metals in free-range chicken, goat and sheep in Ghana and other African countries and assessment of potential risks to human health through consumption  [通常講演]
    Nesta Bortey-Sam, Shouta M.M. Nakayama, Yoshinori Ikenaka, Yared Beyene Yohannes, Elvis Baidoo, Hazuki Mizukawa, Osei Akoto, Mayumi Ishizuka
    SETAC North America 35th Annual Meeting 2014年11月 ポスター発表 Vancouver Convention Centre、Vancouver、Canada
  • Mammalian interspecies differences in xenobiotic phase II reaction  [招待講演]
    Yoshinori Ikenaka, Saengtienchai Aksorn, Mayu Kakehi, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology 2014年09月 口頭発表(招待・特別) Conference Hall of the Kyoto International Foundation, Kyoto, Japan
  • アフリカのゴミ集積場における化学物質の汚染実態と生体への健康影響の評価:ナイロビのダンドラ地域における家畜の重金属汚染の例  [通常講演]
    中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, ヨハンネス ヤレド ベイヤネ, ヤベジョン, 今内 覚, 石塚真由美
    第157回日本獣医学会学術集会 2014年09月 口頭発表(一般) 北海道大学 高等教育推進機構、北海道
  • 日本における猛禽類の鉛中毒被害状況と中毒原因、鉛体内分布の解明  [通常講演]
    石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山 桂, 林 光武, 増田 泰, 坂本健太郎, 石塚真由美
    第157回日本獣医学会学術集会 2014年09月 口頭発表(一般) 北海道大学 高等教育推進機構、北海道
  • ニワトリにおけるDDTの汚染状況と臓器蓄積性の解析  [通常講演]
    伊藤玄悟, 池中良徳, Beyene Yared, 中山翔太, 水川葉月, Vuren Johannes, Wepener Victor, Smit Nico, ynand Vlok, 石塚真由美
    第157回日本獣医学会学術集会 2014年09月 口頭発表(一般) 北海道大学 高等教育推進機構、北海道
  • 鰭脚類においてグルクロン酸抱合酵素が担う異物代謝  [通常講演]
    筧 麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部 薫, 田辺信介, 野見山 桂, 石塚真由美
    第20回日本野生動物医学会つくば大会 2014年09月 ポスター発表 つくば国際会議場、茨城
  • Species differences of UDP-glucuronosyltransferases (UGTs) in Carnivores  [通常講演]
    Mayu Kakehi, Yoshinori Ikenaka, Shouta MM Nakayama, Hazuki Mizukawa, Kensuke, P, Watanabe, Kentaro Sakamoto, Akihiko Wada, Kaoru Hattori, Shinsuke Tanabe, Kei Nomiyama, Mayumi Ishizuka
    12th?International Symposium on Cytochrome P450 Biodiversity and Biotechnology 2014年09月 ポスター発表 Conference Hall of the Kyoto International Foundation、Kyoto、Japan
  • Contamination status and biotransformation of organohalogen compounds in cats and dogs  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Akira Kubota, Hisato Iwata, Yoshinori Ikenaka, Shouta M.M. Nakayama, Shinsuke Tanabe, Mayumi Ishizuka
    12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology 2014年09月 ポスター発表 Conference Hall of the Kyoto International Foundation、Kyoto、Japan
  • Preliminary survey of lead poisoning in raptors from Japan  [通常講演]
    Chihiro Ishii, Shouta M.M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yared, B. Yohannes, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Yasushi Masuda, Kentaro, Q. Sakamoto, Mayumi Ishizuka
    6th?International Toxicology Symposium in Africa 2014年08月 ポスター発表 Johannesburg、South Africa
  • Investigation of heavy metal contamination and assessment of impact on health in Dandora dumping site, Nairobi  [通常講演]
    Hokuto Nakata, Shouta M.M. Nakayama, Hazuki Mizukawa, Yoshinori Ikenaka, Chihiro Ishii, Yared Beyene Yohannes、John Yabe, Satoru Konnai, Mayumi Ishizuka
    6th?International Toxicology Symposium in Africa 2014年08月 ポスター発表 Johannesburg、South Africa
  • Human and ecological health risk assessment of pesticides used for vector control: Current situation and problems of DDT use in Africa  [通常講演]
    Yoshinori Ikenaka, Yared Beyene Yohannes、Lesa Thompson, Shouta M.M. Nakayama, Hazuki Mizukawa, Johannes, J. van Vuren, Victor Wepener, Nico J. Smit, Volk Wynand, Mayumi Ishizuka
    6th?International Toxicology Symposium in Africa 2014年08月 口頭発表(一般) Johannesburg、South Africa
  • Effects of international land-based use of vector-control pesticides on a marine ecosystem: organochlorine analysis of fish from Maputo Bay、 Mozambique  [通常講演]
    Lesa Thompson, Yoshinori Ikenaka, Yared Beyene Yohannes, Shouta M.M. Nakayama, Hazuki Mizukawa, Johannes, J. van Vuren, Victor Wepener, Nico J. Smit, Volk Wynand, Mayumi Ishizuka
    6th International Toxicology Symposium in Africa 2014年08月 口頭発表(一般) Johannesburg、South Africa
  • Distribution of heavy metals in organs of sheep and goat reared in Obuasi, a gold mining town in Ghana  [通常講演]
    Osei Akoto, Nesta Bortey-Sam, Shouta M.M. Nakayama, Yoshinori Ikenaka, Elvis Baidoo, Yared Beyene Yohannes, Hazuki Mizukawa, Mayumi Ishizuka
    6th International Toxicology Symposium in Africa 2014年08月 口頭発表(一般) Johannesburg、South Africa
  • Biomarkers of pollution in Synodontis zambesensis and Hydrocynus vittatus in the lower Pongola river and floodplain  [通常講演]
    Claire Michelle Edwards, Yoshinori Ikenaka, Yared Beyene Yohannes, Shouta M.M, Nakayama, Hazuki Mizukawa, Mayumi Ishizuka, Victor Wepener, Johan J, van Vuren
    6th?International Toxicology Symposium in Africa 2014年08月 口頭発表(一般) Johannesburg、South Africa
  • The impact of environmental stressors on biomarkers in Clarias gariepinus and Oreochromis mossambicus in Ndumo game reserve  [通常講演]
    Mary Tsono Mkhonto, Johan, J, van Vuren, Nico Smit, Yoshinori Ikenaka, Shouta M.M. Nakayama, Hazuki Mizukawa, Yared Beyene Yohannes, Mayumi Ishizuka
    6th?International Toxicology Symposium in Africa 2014年08月 口頭発表(一般) Johannesburg、South Africa
  • EFFECTS OF POLYCHLORINATED BIPHENYLS ON THE EXPRESSION OF CYTOCHROME P450S IN BEAGLE DOGS  [通常講演]
    Saya Tamura, Jean Yoo, Hazuki Mizukawa, Tetsuro Agusa, Masashi Hirano, Kei Nomiyama, Shinsuke Tanabe, Eun-young Kim, Hisato Iwata
    34th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2014) 2014年08月 ポスター発表 Madrid、Spain
  • Contamination status of organohalogen compounds and their metabolites in the blood of pet dogs and cats  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Miyuki Yamamoto, Yoshinori Ikenaka, Shouta M.M. Nakayama, Shinsuke Tanabe, Mayumi Ishizuka
    6th?International Toxicology Symposium in Africa 2014年08月 口頭発表(一般) Johannesburg、South Africa
  • 日本に生息する猛禽類の鉛汚染状況と鉛安定同位体比を指標とした汚染源の解明  [通常講演]
    石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山 桂, 林光 武, 増田 泰, 坂本健太郎, 石塚真由美
    第41回日本毒性学会学術年会 2014年07月 口頭発表(一般) 神戸コンベンションセンター、兵庫
  • ケニアの大規模ゴミ集積場・ダンドラ地域における家畜の化学物質汚染の実態と住民の健康影響評価  [通常講演]
    中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, ヨハンネス ヤレド ベイヤネ, 今内 覚, 石塚真由美
    第41回日本毒性学会学術年会 2014年07月 口頭発表(一般) 神戸コンベンションセンター、兵庫
  • 鰭脚類におけるグルクロン酸抱合酵素の解析  [通常講演]
    筧 麻友, 池中良徳, 中山翔太, 水川葉月, 渡邊研右, 坂本健太郎, 和田昭彦, 服部 薫, 田辺信介, 野見山 桂, 石塚真由美
    第41回日本毒性学会学術年会 2014年07月 口頭発表(一般) 神戸コンベンションセンター、兵庫
  • 鰭脚類を中心とした食肉目において異物代謝を担うグルクロン酸抱合酵素の特徴  [通常講演]
    筧 麻友, 中山翔太, 水川葉月, 池中良徳, 渡邊研右, 坂本健太郎, 和田昭彦, 服部 薫, 田辺信介, 野見山 桂, 石塚真由美
    第41回日本毒性学会学術年会 2014年07月 ポスター発表 神戸コンベンションセンター、兵庫
  • トビケラウオッチ 水生昆虫を用いた河川環境の放射性セシウムモニタリング  [通常講演]
    上野大介, 染谷 孝, 長坂洋光, 楢崎幸範, 稲波 修, 水川葉月, 藤野 毅, 渡邉 泉, 木村 俊介, 小坪 遊, 龍田 希, 仲井邦彦
    第3回環境放射能除染研究発表会 2014年07月 ポスター発表 郡山市民文化センター、福島
  • 食肉目におけるグルクロン酸抱合酵素の解析および種差  [通常講演]
    筧 麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部 薫, 田辺信介, 野見山 桂, 石塚真由美
    第28回北海道薬物作用談話会 2014年07月 口頭発表(一般) 北海道大学、札幌
  • ペット動物における有機ハロゲン化合物の汚染実態解明と水酸化代謝物の動態解析  [通常講演]
    水川葉月, 野見山桂, 中津賞, 池中良徳, 中山翔太, 田辺信介, 石塚真由美
    第28回北海道薬物作用談話会 2014年07月 口頭発表(一般) 北海道大学、札幌
  • Caddisfly Watch Project, bioassessment of radioactive cesium contamination in streams after the Fukushima Daiichi nuclear power station accident using Stenopsyche (Trichoptera: Stenopsychidae)  [通常講演]
    Takeshi Fujino, Daisuke Ueno, Osamu Inanami, Hazuki Mizukawa, Izumi Watanabe, Hiromitsu Nagasaka, Nozomi Tatsuta, Kunihiko Nakai
    The 2nd Symposium of Benthological Society of Asia (BSA) 2014年06月 口頭発表(一般) Busan、Republic of Korea
  • ‐トビケラウォッチ- 被災地における水生昆虫をもちいた放射能モニタリング  [通常講演]
    平野剛史, 上野大介, 染谷 孝, 長坂洋光, 楢崎幸範, 稲波 修, 水川葉月, 藤野 毅, 渡邉 泉, 龍田 希, 仲井邦彦
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • ベクターコントロールのための薬剤散布の生態系およびヒト健康に与える影響評価  [通常講演]
    池中良徳, Yared Yohannes, 中山翔太, 水川葉月, van Vuren Johannes, Wepener Victor, Smit Nico, ynand Vlok, 石塚真由美
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • アフリカのゴミ集積場における化学物質の汚染実態と生体への健康影響の評価:ナイロビのダンドラ地域における重金属汚染の例  [通常講演]
    中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, ヨハンネス ヤレド ベイヤネ, 今内覚, 石塚真由美
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • 日本に生息する猛禽類における鉛汚染状況の解析  [通常講演]
    石井千尋, 池中良徳, 中山翔太, 水川葉月, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • イヌ・ネコにおける微量元素レベルの特性とその暴露起源の探索  [通常講演]
    寳來佐和子, 中北有里子, 水川葉月, 野見山 桂, 渡邉 泉, 田辺信介, 岡本芳晴, 国末達也
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • イヌ・ネコにおけるPCBsおよび水酸化代謝物の脳移行  [通常講演]
    高口倖暉, 野見山 桂, 長野靖子, 水川葉月, 落合真理, 中津 賞, 田辺信介
    第24回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • イヌ・ネコに残留するPCBsの代謝・排泄能とその種間比較  [通常講演]
    野見山 桂, 長野靖子, 水川葉月, 中津 賞, 田辺信介
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • ペットネコにおける有機ハロゲン化合物および水酸化代謝物の汚染実態と曝露源の推定  [通常講演]
    山本恭央, 野見山 桂, 水川葉月, 長野靖子, 田辺信介
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • Quantification of Neonicotinoids in human urine using liquid chromatography/tandem mass spectrometry.  [通常講演]
    Marfo JT, Ikenaka Y, Nakayama SMM, Mizukawa H, Taira K, Fujioka K, Aoyama Y, Akoto O, Ishizuka M
    23rd Symposium on Environmental Chemistry 2014年05月 口頭発表(一般) Kyoto University、 Kyoto、 Japan
  • Residue levels and health risk assessment of organochlorine pesticides (OCPs) in domesticated animals from Egypt.  [通常講演]
    Abdallah FAM, Elsaid AE, Alaa EMAM, Ikenaka Y, Nakayama SMM, Mizukawa H, Yohannes YB, Waleed RG, Mohamed TAbbasy, Ishizuka M
    23rd Symposium on Environmental Chemistry 2014年05月 口頭発表(一般) Kyoto University、 Kyoto、 Japan
  • Health risk assessment of atmospheric polycyclic aromatic hydrocarbons in Kumasi-Ghana.  [通常講演]
    Bortey-Sam N, Ikenaka Y, Nakayama SMM, Akoto O, Yohannes YB, Mizukawa H, Ishizuka M
    23rd Symposium on Environmental Chemistry 2014年05月 口頭発表(一般) Kyoto University、 Kyoto、 Japan
  • GC-ECNI/MS による脳・肝臓中水酸化 PCBsの一斉分析法開発  [通常講演]
    江口哲史, 野見山 桂, 落合真理, 水川葉月, 長野靖子, 神原千佳, 中川勝博, 田中幸樹, 宮川治彦, 田辺信介
    第23回環境化学討論会 2014年05月 ポスター発表 京都大学、京都
  • トビケラウオッチ-水生昆虫を用いた河川環境の放射能調査  [通常講演]
    大坪栄二郎, 平野剛史, 上野大介, 染谷 孝, 長坂洋光, 楢崎幸範, 稲波 修, 水川葉月, 藤野 毅, 渡邉 泉, 亀田 豊, 龍田 希, 仲井邦彦
    平成26年度廃棄物資源循環学会九州支部・研究ポスター会 2014年05月 ポスター発表 福岡大学、福岡
  • ネコ科動物における有機ハロゲン化合物の代謝能解析  [通常講演]
    水川葉月, 池中良徳, 野見山桂, 田辺信介, 宇根有美, 伊藤真輝, 高江洲昇, 長野靖子, 中山翔太, 石塚真由美
    第23回環境化学討論会 2014年05月 口頭発表(一般) 京都大学、京都
  • Biotransformation of organohalogen compounds in the liver microsomes of cats and dogs  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Akira Kubota, Hisato Iwata, Yoshinori Ikenaka, Shinsuke Tanabe, Mayumi Ishizuka
    SOT 53rd Annual Meeting 2014年03月 ポスター発表 Phoenix、Arizona、USA
  • ペットのネコは化学物質のハイリスクアニマルか?  [招待講演]
    水川葉月
    第1回未来設計FS「化学的不均衡を乗り越えるために」研究会 2014年 口頭発表(招待・特別) 京都府
  • Analysis of anthropogenic and naturally occurring brominated phenols in the blood of Japanese pet animals  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Mayumi Ishizuka, Shinsuke Tanabe
    SETAC North America 34th Annual Meeting 2013年11月 ポスター発表 Nashville、Tennessee、USA
  • Characterization and interspecies diversity of xenobiotic metabolism: a study of phase-II conjugation reactions  [通常講演]
    Yoshinori Ikenaka, Aksorn Saengtienchai, Shouta M.M. Nakayama, Hazuki Mizukawa, Mayumi Ishizuka
    5th International Toxicology Symposium in Africa 2013年09月 口頭発表(一般) Kwame Nkrumah University of Science and Technology、Kumasi、Ghana
  • Residue levels and contamination status of organohalogen metabolites in the blood of pet dogs and cats  [通常講演]
    Hazuki Mizukawa, Kei Nomiyama, Susumu Nakatsu, Miyuki Yamamoto, Yoshinori Ikenaka, Mayumi Ishizuka, Shinsuke Tanabe
    1st Sapporo Summer Seminar for One Health (SaSSOH) 2013年09月 口頭発表(一般) Sapporo、Japan
  • イヌ・ネコ肝臓における PCBs 代謝物(OH-PCBs、MeSO2-PCBs)の蓄積特性と代謝能の種間比較  [通常講演]
    長野靖子, 野見山 桂, 水川葉月, 江口哲史, 中津 賞, 田辺信介
    第22回環境化学討論会 2013年07月 口頭発表(一般) 東京農工大学、東京
  • ニホンザルの血液・肝臓に残留する水酸化PCBsの蓄積特性  [通常講演]
    辻沢雄将, 野見山 桂, 水川葉月, 落合真理, 谷地森秀二, 葦田恵美子, 田辺信介
    第22回環境化学討論会 2013年07月 口頭発表(一般) 東京農工大学、東京
  • 野生げっ歯類におけるワルファリン抵抗性機構  [通常講演]
    池中良徳, 田中和之, 中山翔太, 春成常仁, 谷川力, 水川葉月, 石塚真由美
    第22回環境化学討論会 2013年07月 口頭発表(一般) 東京農工大学、東京
  • イヌ、ネコの肝ミクロソームを用いたPCBsおよびPBDEs代謝能の種間比較  [通常講演]
    水川葉月, 野見山桂, 中津賞, 久保田彰, 石塚真由美, 岩田久人, 田辺信介
    第22回環境化学討論会 2013年07月 口頭発表(一般) 東京農工大学、東京
  • 陸棲哺乳類の血中に残留するハロゲン化フェノール類の種間比較  [通常講演]
    水川葉月, 野見山 桂, 中津 賞, 谷地森秀二, 林 光武, 田代 豊, 長野靖子, 田辺信介, 石塚真由美
    平成25年度(2013年度) P450、UGT/SULT研究会 2013年06月 口頭発表(一般) フェニックス・シーガイア・リゾート、宮崎

MISC

  • 鈴木 智裕, 池中 良徳, 中山 翔太, 水川 葉月, 一瀬 貴大, 佐々木 東, 笹岡 一慶, 石塚 真由美 日本獣医学会学術集会講演要旨集 162回 501 -501 2019年08月
  • 白尾 大樹, 池中 良徳, 一瀬 貴大, 中山 翔太, 佐々木 東, 永田 矩之, 水川 葉月, 滝口 満喜, 石塚 真由美 日本獣医学会学術集会講演要旨集 162回 470 -470 2019年08月
  • 水川葉月, NGYUEN Hoa Thanh, 岩田久人, 野見山桂, 池中良徳, 中山翔太, 横山望, 市居修, 滝口満喜, KHIDKHAN Kraisiri, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.1C‐08 2019年06月
  • 田中厚資, 綿貫豊, 高田秀重, 石塚真由美, 山下麗, 水川薫子, 水川葉月, 池中良徳, 中山翔太 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.P‐031 2019年06月
  • 白尾大樹, 池中良徳, 一瀬貴大, 中山翔太, 佐々木東, 永田矩之, 水川葉月, 滝口満善, 石塚真由美 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.1A‐10 2019年06月
  • 田中啓介, 野見山桂, 水川葉月, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 28th ROMBUNNO.1C‐09 2019年06月
  • 打田茉由果, 石塚真由美, 池中良徳, 水川葉月, 中山翔太, 坂東武治, 田村力, 小西健志, 鈴木一平, 三谷曜子 日本水産学会大会講演要旨集 2019 162 2019年03月
  • W. Pheiffer, N. Bortey-Sam, Y. Ikenaka, Y. Ikenaka, S. M.M. Nakayama, H. Mizukawa, M. Ishizuka, N. J. Smit, R. Pieters African Journal of Aquatic Science 43 305 -312 2018年09月 
    © 2018, © 2018 NISC (Pty) Ltd. The concentrations of selected hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) were determined in the bile of the African sharptooth catfish Clarias gariepinus from impoundments in the urban impacted Klip River system in Soweto, South Africa. Fish were sampled from three impoundments (Lenasia, Fleurhof, and Orlando dams) during the early high-flow season (September/October) of 2013. Biliary OH-PAHs were analysed using a high-pressure liquid chromatograph coupled to a tandem mass spectrometer (HPLC-MS/MS). Seven of the thirteen targeted metabolites were present in the fish of Soweto. The ΣOH-PAHs ranged between 0.1 and 1 876 ng ml−1, with greatest ΣOH-PAH mean at Orlando (947 ng ml−1) followed by Fleurhof (371 ng ml−1). The most dominant metabolite in the sampling area was 2–,3–OH fluorene, ranging between not detected and 1 429 ng ml−1, with the greatest mean at Orlando (709 ng ml−1). PAH metabolites quantified in C. gariepinus most likely originated from the sediments. The hepatosomatic index of the C. gariepinus increased proportionally with the biliary OH-PAH concentrations. To the authors’ knowledge this data on biliary OH-PAH for fish is the first for South Africa.
  • 庄子晶子, ELLIOTT K, ARIS‐BROSOU S, 水川葉月, 中山翔太, 池中良徳, 石塚真由美, 桑江朝比呂, 渡辺謙太, 綿貫豊 日本鳥学会大会講演要旨集 2018 97 2018年09月
  • XAFSを用いたザンビア鉛汚染地域の生物及び環境試料における鉛の化学形態の解明
    豊巻治也, 中山翔太, 中田北斗, 藤森崇, 塩田憲司, Yabe John, Muzandu Kaampwe, Beyene Yared, 池中良徳, 水川葉月, 石塚真由美 第21回XAFS討論会 研究発表ペーパー・要旨(全国大会,その他学術会議) 2018年09月
  • 佐野花澄, 池中良徳, 近藤誉充, 川合佑典, 中山翔太, 水川葉月, 石塚真由美 日本獣医学会学術集会講演要旨集 161st 469 2018年08月
  • 山田尚輝, 中山翔太, 水川葉月, 小笠原浩平, 渡邊有希子, 齊藤慶輔, 伊藤真輝, 大澤夏生, 竹中康進, 小松謙之, 千葉茜, 内田義崇, 池中良徳, 石塚真由美 日本獣医学会学術集会講演要旨集 161st 469 2018年08月
  • 三津橋嵩史, 池中良徳, 池中良徳, 中山翔太, 水川葉月, 一瀬貴大, SAENGTIENCHAI Aksorn, 河合正人, 南保泰雄, 石塚真由美 日本獣医学会学術集会講演要旨集 161st 467 2018年08月
  • 武田 一貴, 池中 良徳, 田中 和之, 中山 翔太, 谷川 力, 水川 葉月, 石塚 真由美 日本毒性学会学術年会 45 (0) P -71 2018年08月 
    <p> 野生げっ歯類はレプトスピラやハンタウイルス感染症、ペストなど種々の人獣共通感染症を媒介するため殺鼠剤による駆除は不可欠である。しかしワルファリン(Wf)など殺鼠剤に対し抵抗性を有する個体群が殺鼠剤使用頻度の高い世界各地の都市に出現している。現在抵抗性ラットに有効な防除策は乏しく、抵抗性獲得機序の解明と新規防除策の提案は急務である。</p><p> これまでWf抵抗性の原因はWfの標的分子ビタミンKエポキシド還元酵素の遺伝子変異だと考えられてきたが、発表者が東京の殺鼠剤抵抗性ラットでWf肝灌流試験を実施した所、肝臓でのWf代謝が亢進していた。このため、抵抗性にWfの代謝・排泄も寄与することが示唆された。また、肝臓から薬物代謝酵素シトクロムP450(CYPs)を含む酵素画分を調製しWf代謝試験を実施すると、ミクロソームへNADPHを添加した所CYPsの酵素活性に大きな差は認められなかったが、S9画分へNADP+を添加するとCYPsによる代謝活性が抵抗性群で大きく上昇した。このため、肝灌流で確認された高代謝能はCYPs自体の変化ではなく、その活性に必要なNADPH産生能に起因するということが示唆された。また、実際に抵抗性ラットS9画分ではNADPHの産生が亢進していた。</p><p> このため、抵抗性ラットで確認された高NADPH産生能の責任分子を特定するため、肝臓でNADPH産生を主に担うペントースリン酸経路のNADPH産生酵素、グルコース6リン酸脱水素酵素(G6PD)について、シークエンス解析とウェスタンブロットによる発現量の定量を行った。しかしG6PD発現量自体には有意な差は認められず、アミノ酸配列においても変化は認められなかった。</p>
  • 中山 翔太, ジアンボ ゴルデン, 栗谷 豪, 中川 光弘, 水川 葉月, 石塚 真由美, ヤベ ジョン, 池中 良徳, ムザンド カアンプウェ, チョーンゴ ケネディ, 中田 北斗, 豊巻 治也, ベイエネ ヤレド, カタバ アンドリュー 日本毒性学会学術年会 45 (0) P -35 2018年08月 
    <p>発表者らは、2008年よりアフリカのザンビア共和国カブウェ市における鉛汚染問題に関わる研究を進めてきた。当該の鉛・亜鉛鉱床地域では、1900年頃から鉱床活動が行われているが、低効率の精錬技術による多くの不純物を含む鉱さいの排出および不適切な廃棄により、地質中の鉛やカドミウムが環境に拡散している。我々の先行研究により、家畜(ニワトリ、ヤギ、ウシ)およびイヌ、野生ラットなどの動物における血中・臓器中の高濃度の鉛蓄積を確認した。さらに、鉱床周辺に住む子供300名の血中鉛濃度は、対象のほぼ全員が研究当時の基準値である5 µg/dLを超過する深刻な状況であることを解明した。この研究結果を受けて、特に子供における治療や地域の環境汚染レベルの低減を目的としたプロジェクト(<u>KA</u>bwe <u>M</u>ine <u>P</u>ollution <u>A</u>melioration <u>I</u>nitiative ~KAMPAI Project~)を2016年より実施している。本発表では、より広範囲のエリアにおける汚染状況の解明や、鉛の暴露経路解明を目的としたプロジェクトの一部結果を発表する。</p><p>ザンビア大学、ザンビア国保健省および所属機関における研究倫理許可を取得し、鉱床付近およびそれ以外のエリアも対象とした約500世帯(父、母、子供)の合計1250名における血液・血漿・尿および子供の糞便試料を採取した。血中鉛濃度は、オンサイトで測定可能なLeadCareⅡを用いて、現地クリニックにおいて測定し、鉛汚染の広がっている範囲の概要を掴むことができた。予想に反して、鉱床から離れた地域でも高濃度の血中鉛が検出される対象者の存在も明らかになった。今後は、血液や糞便における金属濃度および鉛安定同位体比解析により鉛の人への暴露経路を解明し、環境改善手法を検討する。さらに、100年間以上にも及ぶ長期慢性暴露により、動物や人におけるゲノム・エピゲノム修飾、腸内細菌叢コミュニティ変化などの可能性を予想している。近年では、鉛の毒性発現には閾値を設定できないとされているが、長期慢性暴露に伴う毒性発現メカニズムを解明し、効果的な予防法・治療法開発に貢献したい。</p>
  • 近藤 誉充, 池中 良徳, 中山 翔太, 水川 葉月, 三谷 曜子, 野見山 桂, 田辺 信介, 石塚 真由美 日本毒性学会学術年会 45 (0) P -60 2018年08月 
    <p>アザラシやネコ科を含む食肉目動物は生態系高次栄養段階に位置し、食物連鎖を介した生物濃縮により残留性の高い有機化合物が高濃度で生体内に蓄積している。化学物質代謝酵素はこれら化学物質の解毒を担う酵素である。その中でも特に第II相抱合酵素はCytochrome P450等の代謝を受けた化学物質をさらに代謝する酵素であり、発がん性物質を含む多くの化学物質がP450の代謝を受けた後に代謝的活性化を示すため、第II相酵素は特に解毒に重要な酵素である。しかし多くの食肉目動物で第II相抱合酵素の情報は皆無であり、特に基本的情報である遺伝的性状や酵素活性の種差の情報が欠如している。そこで本研究は主要な第II相抱合酵素であるグルクロン酸転移酵素(UGT)および硫酸転移酵素(SULT)の遺伝子性状およびin vitro酵素活性の解明を目的とした。遺伝的性状解析では遺伝子データベースやシークエンス解析による遺伝子情報から系統解析、および遺伝子コード領域の種差を解明した。In vitro活性解析ではネコ、イヌ、ラット、および鰭脚類(カスピカイアザラシ、ゼニガタアザラシ、トド、キタオットセイ)の肝ミクロソームおよびサイトゾルを用いて種々の分子種特異的な基質(Lorazepam:UGT2B分子種, Estradiol:SULT1E1等)に対する酵素活性を測定した。UGTに関して、系統解析の結果から食肉目で特に重要と推定される2B31分子種の存在が明らかとなった。また、食肉目の中でもイヌでは3つのUGT2B31を持つのに対して、ネコ科動物では2B分子種が存在せず、鰭脚類でも1つの分子種しか持たないことが明らかとなった。さらにin vitro活性もイヌと比較してネコ及びアザラシ科で非常に低い活性が確認された。SULTに関しては、鰭脚類でエストロゲン代謝に重要なSULT1E1分子種が遺伝的に欠損しており、in vitro活性も低いことが解明された。これらの結果から食肉目動物の中でもとくに鰭脚類やネコ科動物では第II相抱合酵素による解毒能が弱く、種々の化学物質に対して感受性が強い可能性が示唆された。</p>
  • 中山翔太, 森田鮎子, 池中良徳, 石井千尋, 水川葉月, 武田一貴, 川合佑典, 齊藤慶輔, 渡邊有希子, 大澤夏生, 伊藤真輝, 石塚真由美 日本野生動物医学会大会・講演要旨集 24th 150 2018年08月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美 日本野生動物医学会大会・講演要旨集 24th 151 2018年08月
  • 石井千尋, 池中良徳, 池中良徳, 中山翔太, 齊藤慶輔, 渡辺有希子, 小笠原浩平, 水川葉月, 石塚真由美 日本野生動物医学会大会・講演要旨集 24th 114 2018年08月
  • 中山翔太, 池中良徳, 石井千尋, 水川葉月, 川合佑典, 平野なぎ沙, 齊藤慶輔, 渡邊有希子, 大澤夏生, 伊藤真輝, 中村亮平, 石塚真由美 日本野生動物医学会大会・講演要旨集 24th 138 2018年08月
  • 佐々木 東, 中山 翔太, 大田 寛, 大菅 辰幸, 森下 啓太郎, 水川 葉月, 池中 良徳, 石塚 真由美, 滝口 満喜 日本獣医学会学術集会講演要旨集 161回 429 -429 2018年08月
  • 中山翔太, YABE John, 池中良徳, MUZANDU Kaampwe, CHOONGO Kennedy, 中田北斗, 豊巻治也, BEYENE Yared, KATABA Andrew, ZYAMBO Golden, 栗谷豪, 中川光弘, 水川葉月, 石塚真由美 Journal of Toxicological Sciences 43 (Supplement) S226 2018年06月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 野見山桂, 田辺信介, 石塚真由美 Journal of Toxicological Sciences 43 (Supplement) S238 2018年06月
  • 武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 Journal of Toxicological Sciences 43 (Supplement) S244 2018年06月
  • 一瀬貴大, 池中良徳, 池中良徳, SAENGTIENCHAI Aksorn, 中山翔太, 水川葉月, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.P‐086 2018年05月
  • 池中良徳, THOMPSON Lesa, DARWISH Wageh, YOHANNES Yared, VAN VUREN Johan, SMIT Nico, WEPENER Victor, 中山翔太, 水川葉月, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐04 2018年05月
  • 豊巻治也, YABE John, 中山翔太, YARED Beyene, MUZANDU Kaampwe, 水川葉月, 池中良徳, 中田北斗, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.3C‐06(PA‐54) 2018年05月
  • 武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2B‐01(PA‐22) 2018年05月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐09(PA‐18) 2018年05月
  • 島崎真琴, 野見山桂, 水川葉月, SAENGTIENCHAI Aksorn, NGAMCHIRTTAKUL Araya, PENCHAREE Disdanai, 星野光希, 山本恭央, 高口倖暉, 石塚真由美, 池中良徳, 中山翔太, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐03(PA‐14) 2018年05月
  • 池中良徳, 一瀬貴大, NIMAKO Collins, 市川剛, 水川葉月, 中山翔太, 有薗幸司, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.1C‐16 2018年05月
  • 野見山桂, 江口哲史, 西川博之, 水川葉月, 山本恭央, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 田辺信介 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐11 2018年05月
  • 銅谷理緒, 中山翔太, 中田北斗, 豊巻治也, YABE John, MUZANDU Kaampwe, BEYENE Yared, KATABA Andrew, ZYAMBO Golden, 水川葉月, 池中良徳, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.3C‐05(PA‐53) 2018年05月
  • 中山翔太, 豊巻治也, YABE John, MUZANDU Kaampwe, 中田北斗, 栗谷豪, 中川光弘, 水川葉月, 池中良徳, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.3C‐08 2018年05月
  • 水川葉月, KHIDKHAN Kraisiri, 高口倖暉, 横山望, 市居修, 滝口満喜, 笹岡一慶, 池中良徳, 池中良徳, 中山翔太, TSEND‐AYUSH Sainnoxoi, 野見山桂, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.2A‐10 2018年05月
  • 田中啓介, 野見山桂, 水川葉月, 高口倖暉, 田上瑠美, 横山望, 市居修, 滝口満喜, 笹岡一慶, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 27th ROMBUNNO.1A‐03(PA‐13) 2018年05月
  • 打田茉由果, 鈴木一平, 石塚真由美, 池中良徳, 中山翔太, 水川葉月, 田村力, 坂東武治, 小西健志, 三谷曜子 日本水産学会大会講演要旨集 2018 44 2018年03月
  • 高口 倖暉, 中山 翔太, 池中 良徳, 石塚 真由美, 岩田 久人, 国末 達也, 田辺 信介, 野見山 桂, 西川 博之, 水川 葉月, 田上 瑠美, 草木 桃子, 横山 望, 市居 修, 滝口 満喜 日本毒性学会学術年会 45 (0) P -72 2018年 

    近年、ペット動物に対するポリ塩化ビフェニル(PCBs)および水酸化代謝物(OH-PCBs)の曝露による甲状腺ホルモン(THs)の恒常性への影響が注目されている。本研究ではPCBs曝露がペット動物のTHs恒常性に及ぼす影響を明らかにするため、イヌ・ネコのPCBs投与試験を実施し、PCBs曝露に伴う血清中THs濃度の変化を解析した。

    PCBs投与後から5日間、継続的に血清を採取し、THs濃度の変化を解析した結果、ネコ血清中THs濃度は、総THs、遊離型THsともに対照群と投与群の間に有意な変化は認められなかった。一方、イヌ血清中総THs濃度は投与群において、総L-サイロキシン(T4)と総3,5,3'-トリヨード-L-サイロニン(T3)の減少傾向がみられ、総PCBs濃度は総T4、総T3濃度と有意な負の相関を示した(p < 0.01)。一方、遊離型T4、遊離型T3濃度では、対照群と投与群の間に経時的な変化は認められなかったが、曝露後48、96時間目で有意に増加した(p < 0.05)。加えて、遊離型T4濃度は総OH-PCBs濃度と正の相関を示し(p < 0.01)、異性体別ではT4様構造の高塩素化OH-PCBsで同様の傾向が認められた。上記の結果および先行研究から、PCBs曝露によるイヌ血清中THsへの影響を推察した。PCBsがT4様構造のOH-PCBsへ代謝されてTHs輸送タンパクに競合結合し、結合できない遊離型T4が血中に増加することで、THsの臓器・組織への取り込み量が増加したと予測される。その結果、イヌ肝臓中のTHs濃度は増加し、PCBs曝露により肝臓中のAhR・CARが過剰に誘導されることで、UGTおよびSULTが誘導され、THsの抱合化を促進することで体外排泄量が増加し、血清中総T4、T3が減少したものと推察された。

  • 池中良徳, 宮原裕一, 一瀬貴大, 中山翔太, 水川葉月, 市川剛, 遠山千春, 石塚真由美 日本内分泌かく乱化学物質学会研究発表会要旨集 20th 44 2017年12月
  • 中山 翔太, 水川 葉月, 池中 良徳, 石塚 真由美 日本野生動物医学会誌 = Japanese journal of zoo and wildlife medicine 22 (4) 69 -72 2017年12月
  • 石塚真由美, 中山翔太, 水川葉月, 池中良徳 Endocrine Disrupter News Letter 20 (3) 4 2017年12月
  • 水川葉月, 高居名菜, 野見山桂, 高口倖暉, 横山望, 市居修, 滝口満喜, 池中良徳, 池中良徳, 中山翔太, 石塚真由美 日本内分泌かく乱化学物質学会研究発表会要旨集 20th 78 -78 2017年12月
  • 庄子晶子, ELLIOTT K, ARIS‐BROSOU S, 水川葉月, 中山翔太, 池中良徳, 石塚真由美, 桑江朝比呂, 渡辺謙太, 綿貫豊 日本鳥学会大会講演要旨集 2017 70 2017年09月
  • 新屋惣, 池中良徳, 中山翔太, 水川葉月, 伊藤真輝, 高江洲昇, 齊藤慶輔, 渡邉有希子, 石塚真由美 日本鳥学会大会講演要旨集 2017 105 2017年09月
  • 石井千尋, 池中良徳, 川合佑典, 市居修, 中山翔太, 水川葉月, 石塚真由美 日本鳥学会大会講演要旨集 2017 100 2017年09月
  • 武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 日本獣医学会学術集会講演要旨集 160th 504 2017年08月
  • 佐々木 東, 中山 翔太, 森下 啓太郎, 中村 健介, 大田 寛, 水川 葉月, 池中 良徳, 石塚 真由美, 滝口 満喜 日本獣医学会学術集会講演要旨集 160回 461 -461 2017年08月
  • 大原 優美子, 矢吹 映, 市居 修, 水川 葉月, 横山 望, 中村 麗菜 日本獣医学会学術集会講演要旨集 160回 473 -473 2017年08月
  • 池中良徳, 池中良徳, 宮原裕一, 一瀬貴大, 八木橋美緒, 中山翔太, 水川葉月, 遠山千春, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.3C‐03 2017年06月
  • 武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.P‐218 2017年06月
  • 一瀬貴大, 池中良徳, 池中良徳, 藤岡一俊, 平久美子, 中山翔太, 水川葉月, 八木橋美緒, 有薗幸司, 加藤恵介, 高橋圭介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.P‐093 2017年06月
  • 水川葉月, 高居名菜, 横山望, 市居修, 滝口満喜, 野見山桂, 高口倖暉, 西川博之, 池中良徳, 池中良徳, 中山翔太, SAINNOXOI Tsend‐ayush, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐01 2017年06月
  • 高口倖暉, 野見山桂, 西川博之, 水川葉月, 田上瑠美, 芳之内結加, 横山望, 市居修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 岩田久人, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐02 2017年06月
  • 水川葉月, 野見山桂, 池中良徳, 池中良徳, 中山翔太, SAENGTIENCHAI Aksorn, SAINNOXOI Tsendayush, 横山望, 笹岡一慶, 市居修, KHIDKHAN Kraisiri, 高居名菜, 滝口満喜, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐03 2017年06月
  • 田中啓介, 野見山桂, 水川葉月, 高口倖暉, 田上瑠美, SAENGTIENCHAI Aksorn, SAINNOXOI Tsend‐ayush, 横山望, 市居修, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐04 2017年06月
  • 野見山桂, 西川博之, 水川葉月, 高口倖暉, SAINNOXOI Tsend‐ayush, 横山望, 市居修, 滝口満喜, 石塚真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 26th ROMBUNNO.2A‐05 2017年06月
  • 武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 Journal of Toxicological Sciences 42 (Supplement) S219 2017年06月
  • 水川 葉月, 高居 名菜, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 高口 倖暉, 西川 博之, 池中 良徳, 中山 翔太, Sainnoxoi Tsend-ayush, 田辺 信介, 石塚 真由美 The Journal of Toxicological Sciences 42 (Suppl.) S236 -S236 2017年06月
  • 水川葉月, 野見山桂, 田辺信介, 池中良徳, 池中良徳, 中山翔太, 石塚真由美 Journal of Toxicological Sciences 42 (Supplement) S36 2017年06月
  • 打田茉由果, 伊藤慶造, 石塚真由美, 池中良徳, 中山翔太, 水川葉月, 坂東武治, 田村力, 小西健志, 鈴木一平, 三谷曜子 日本水産学会大会講演要旨集 2017 24 2017年03月
  • DARWISH Wageh Sobhy, IKENAKA Yoshinori, NAKAYAMA Shouta M.M, MIZUKAWA Hazuki, ISHIZUKA Mayumi 日本毒性学会学術年会 44 (0) O -15 2017年 
    Polycyclic aromatic hydrocarbons (PAHs) are wide spread as environmental pollutants, which can be generated during the preparation of food. Food is the main source of exposure to PAHs for people who do not smoke. Epidemiological studies indicate that diets rich in fruits and vegetables can be associated with lower risks of numerous diseases and cancers. However, the exact mechanisms behind these effects are still unclear. The objectives of this study were firstly to estimate PAHs contents in some heat-treated meat samples collected from different localities in Egypt. Secondly, to investigate the mutagenic activities for some of the formed PAHs. Finally, to investigate the protective effects and mechanism of action of some micronutrients against the adverse effects of PAHs. <br> Polycyclic aromatic hydrocarbons were measured in heat-treated meat collected from different localities in Egypt using HPLC. Mutagenic activities of some formed PAHs were examined using Ames Salmonella typhimurium mutagenicity assay. Human HepG2 cells were exposed to environmental-relevant concentrations of some formed PAHs. Modulation of xenobiotic metabolizing enzymes (XMEs) mRNA expressions was investigated using real-time qPCR.<br> The results of this study indicated the formation of different PAHs in heat-treated meat with variable concentrations. Some of the formed PAHs had clear mutagenic activities such as benzo[a]pyrene. Obtained results declare the protective effects of carotenoids like β-carotene and retinoids like retinol against B[a]P induced adverse effects. Modulation of XMEs is proposed to be a possible mechanism for this protection. In conclusion, consumption of β-carotene and retinol in the areas of high PAH pollution is recommended.
  • 池中 良徳, 加藤 恵介, 遠山 千春, 石塚 真由美, 宮原 裕一, 一瀬 貴大, 八木橋 美緒, 中山 翔太, 水川 葉月, 平 久美子, 有薗 幸司, 高橋 圭介 日本毒性学会学術年会 44 (0) O -20 2017年 
    ネオニコチノイド系殺虫剤は、哺乳類における体内蓄積性は短く、昆虫とヒトのニコチン受容体に対する親和性の違いから、ヒトに対する毒性は相対的に低いため、一定の基準以下であれば、日常生活においてその毒性は無視できると考えられている。しかし、日本では諸外国と比べ数倍~数十倍と果物や野菜、茶葉における食品残留基準値が高く設定されていること、また、記憶・学習などの脳機能に及ぼす影響をはじめ、発達神経毒性には不明な点が多いことなどから、健康に及ぼす懸念が払拭できていない。とりわけ、感受性が高いこどもたちや化学物質に過敏な人々の健康へのリスクを評価するためには、ネオニコチノイドが体内にどの程度取り込まれているかを把握することがまず必要である。そこで本調査では、長野県上田市の松くい虫防除が行われている地域の住民のうち、感受性が高いと考えられる小児(3歳~6歳)から尿を採取し、尿中のネオニコチノイドおよびその代謝物を測定することで、曝露評価を行う事を目的とした。当該調査では、松枯れ防止事業に用いる薬剤(エコワン3フロワブル、主要成分:Thiacloprid)の散布時期の前後に、46人の幼児から提供された尿試料中のネオニコチノイドとその代謝産物を測定した。また、同時に大気サンプルもエアーサンプラーを用いて採取し、分析に供した。分析した結果、Thiaclopridは検出頻度が30%程度であり、濃度は
  • 石井 千尋, 中山 翔太, 水川 葉月, 池中 良徳, 石塚 真由美 The Japanese journal of clinical toxicology = 中毒研究 : 日本中毒学会機関誌 30 (4) 363 -367 2017年
  • L. A. Thompson, Y. Ikenaka, Y. B. Yohannes, J. J. van Vuren, V. Wepener, N. J. Smit, W. S. Darwish, S. M. M. Nakayama, H. Mizukawa, M. Ishizuka FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 34 (11) 1959 -1969 2017年 
    Organochlorine pesticides such as dichlorodiphenyltrichloroethane (DDT) have been used in agriculture and for disease control purposes over many decades. Reports suggest that DDT exposure may result in a number of adverse effects in humans. In the KwaZulu-Natal Province of South Africa, DDT is sprayed annually in homes (indoor residual spraying) to control the mosquito vector of malaria. In the northern part of the Province, samples of free-range chicken meat (n=48) and eggs (n=13), and commercially produced chicken meat (n=6) and eggs (n=11), were collected and analysed. Of the free-range chicken meat samples, 94% (45/48) contained DDTs (sigma DDTs median 6.1ng/g wet weight [ww], maximum 79.1ng/g ww). Chicken egg contents were also contaminated (sigma DDTs in free-range eggs median 9544ng/g ww, maximum 96.666ng/g ww; and in commercial eggs median 1.3ng/g ww, maximum 4.6ng/g ww). The predominant DDT congener detected was p,p'-DDE in both free-range meat (>63%) and eggs (>66%), followed by p,p'-DDT and then p,p'-DDD. Based on estimated daily intake values, calculated human risk ratio (carcinogenic) values were >1 for DDTs detected in both free-range chicken products. Consumption of free-range eggs poses a particularly high health risk.
  • 石井千尋, 中山翔太, 池中良徳, ANDREW Kataba, 齊藤慶輔, 渡辺有希子, 槇納好岐, 松川岳久, 久保田章乃, 横山和仁, 水川葉月, 平田岳史, 石塚真由美 日本野生動物医学会大会・講演要旨集 23rd 81 2017年
  • 新屋惣, 池中良徳, 中山翔太, 石井千尋, 水川葉月, 伊藤真輝, 高江洲昇, 大澤夏生, 綿貫豊, 石塚真由美 日本野生動物医学会大会・講演要旨集 23rd 125 2017年
  • 石塚真由美, 中山翔太, 水川葉月, 池中良徳 日本薬学会年会要旨集(CD-ROM) 137th ROMBUNNO.S45‐4 2017年
  • 武田一貴, 池中良徳, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 日本生化学会大会(Web) 90th ROMBUNNO.3AT12‐14(3P‐0236) (WEB ONLY) 2017年
  • 水川 葉月, 中山 翔太, Tsend-ayush SAINNOXOI, 田辺 信介, 石塚 真由美, 高居 名菜, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 高口 倖暉, 西川 博之, 池中 良徳 日本毒性学会学術年会 44 (0) P -55 2017年 
    有機ハロゲン化合物の一種であるポリ塩化ビフェニル(PCBs)は甲状腺ホルモン(THs)の恒常性かく乱が懸念されており、実際、ヒトやげっ歯類においてはPCBs暴露により血中THs濃度の低下が認められている。ヒトの身近な動物であるネコは餌などを介してPCBsに暴露していると懸念され、THs恒常性に何らかの悪影響をもたらすことが予想される。本研究では、ネコの甲状腺機能に対するPCBsの影響を解析するため、in vivo投与試験したネコを用いて甲状腺機能への影響を解析した。血中のTHs濃度(総T4、総T3、遊離T4、遊離T3、リバースT3)を解析した結果、対照群と暴露群の間に有意な差は見られなかった。また、THsの恒常性に関与する遺伝子(甲状腺ホルモン受容体αおよびβ、脱ヨード酵素type1)の肝臓中発現量やTHs代謝に関わる酵素であるグルクロン酸抱合酵素(UGT1A1)の活性を調べたところ、対照群と暴露群の間に有意な差は見られなかった。
    本実験結果では、ネコにおいてPCBs短期暴露によるTHsへの影響が認められなかったが、日本のペットネコにおけるモニタリング調査では、血中のTHs濃度とPCBs濃度との間に負の相関が見られており、有機ハロゲン化合物によるTHsかく乱が懸念されている。また、本研究試料のメタボローム解析結果では酸化ストレスの発現、抗酸化物質、電子伝達物質の生産阻害などが示唆されており、PCBsや水酸化代謝物による内分泌撹乱作用が心配される。THsの恒常性は非常に複雑な機構のもと維持されていることが知られており、負のフィードバック機構に加え、THsの活性化や代謝、排泄の調整にも多くの遺伝子やトランスポーター、酵素など様々な要因が関わっていることから、ネコは長期的かつ慢性的に化学物質に暴露されることで、甲状腺機能などへの悪影響が現れると考えられ、内分泌撹乱作用の作用機序について解析を進めることが重要である。
  • 石塚真由美, 中山翔太, 水川葉月, 池中良徳 日本哺乳類学会大会プログラム・講演要旨集 2016 56 2016年09月
  • 石塚真由美, 中山翔太, 水川葉月, 池中良徳 日本獣医学会学術集会講演要旨集 159th 293 2016年08月
  • 近藤誉充, 池中良徳, 中山翔太, 水川葉月, SAENGTIENCHAI Aksorn, BEYENE Yared, 坂本健太郎, 和田昭彦, 服部薫, 三谷曜子, 田辺信介, 野見山桂, 石塚真由美 日本獣医学会学術集会講演要旨集 159th 493 2016年08月
  • 栂尾正雄, 中山翔太, YABE John, 池中良徳, 中田北斗, 石井千尋, 水川葉月, BEYENE Yared, SAENGTIENCHAI Aksorn, 石塚真由美 日本獣医学会学術集会講演要旨集 159th 494 2016年08月
  • 中村 麗菜, 矢吹 映, 市居 修, 水川 葉月, 横山 望, 大和 修 日本獣医学会学術集会講演要旨集 159回 462 -462 2016年08月
  • 池中良徳, 池中良徳, 藤岡一俊, 平久美子, 中山翔太, 水川葉月, 一瀬貴大, MARFO Jemima Tiwaa, 有薗幸司, 加藤恵介, 石坂真澄, 馬場浩司, 川上智規, 石塚真由美 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐07 2016年06月
  • 水川葉月, 前原美咲, 横山望, 市居修, 滝口満喜, 野見山桂, 西川博之, 池中良徳, 中山翔太, 高口倖暉, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐13 2016年06月
  • 西川博之, 野見山桂, 水川葉月, 横山望, 市居修, 滝口満喜, 石塚真由美, 池中良徳, 中山翔太, 江口哲史, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐12 2016年06月
  • 野見山桂, 山本恭央, 水川葉月, 江口哲史, 滝口満喜, 中山翔太, 池中良徳, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.1A‐14 2016年06月
  • 野見山桂, 高口倖暉, 江口哲史, 水川葉月, YOO Jean, 田村沙弥, 忍穂井智子, 岩田久人, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 25th ROMBUNNO.P‐030 2016年06月
  • 新屋惣, 池中良徳, 中山翔太, 石井千尋, 水川葉月, 齊藤慶輔, 渡邊有希子, 伊藤真輝, 高江洲昇, 石塚真由美 日本野生動物医学会大会・講演要旨集 22nd 97 2016年
  • 武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 日本分子生物学会年会プログラム・要旨集(Web) 39th ROMBUNNO.3LBA‐070 (WEB ONLY) 2016年
  • 池中良徳, 池中良徳, 筧麻友, 水川葉月, 中山翔太, 石塚真由美 日本薬学会年会要旨集(CD-ROM) 136th ROMBUNNO.S66-3 2016年
  • 武田 一貴, 池中 良徳, 田中 和之, 谷川 力, 中山 翔太, 水川 葉月, 石塚 真由美 日本毒性学会学術年会 43 (0) P -89 2016年 
    ワルファリンは抗凝血作用を示す化学物質であり、その薬効はビタミンK依存性血液凝固因子合成に不可欠なビタミンKエポキシド還元酵素 (VKOR)の阻害により発揮される。ワルファリンを含む種々の抗凝血薬は殺鼠剤として広く用いられているが、世界中の都市部でワルファリンに対し抵抗性を持つ野生齧歯類の棲息が確認されており、東京では棲息数の80%を占める地域すら存在する。これら野生齧歯類は重篤な人獣共通感染症を媒介するため、抵抗性獲得機序の解明は急務であるといえる。抵抗性の原因としてワルファリンの標的分子:VKORに遺伝子変異が生じ、それに伴いVKORのワルファリン感受性が低下する事が報告されている。しかしVKOR変異が生体レベルでどの程度抵抗性へ寄与するか不明な点も多くVKOR以外の抵抗性獲得機序が疑われている。そこでVKOR変異以外の要因を探索するため、筆者らは東京由来ワルファリン抵抗性野生クマネズミクローズドコロニーを確立し、その個体群を用いワルファリン経口投与後体内薬物動態解析を行った。その結果、抵抗性群ではワルファリン投与後のAUC(血漿中濃度-時間曲線下面積)、肝臓腎臓中蓄積濃度が感受性群に比べ有意に低く、一方で消失速度を示すクリアランスは有意に高かった。またワルファリンの代謝産物である水酸化ワルファリンの血漿中濃度は、抵抗性群において投与後1時間と非常に迅速に最高濃度に達し(感受性群では投与後6時間前後)、尿中への排泄量も水酸化体の一分子種(4’水酸化体)で有意に増加していた。このワルファリン代謝を担っているシトクロムP450分子種の肝ミクロソーム中タンパク発現量は抵抗性群では有意に高かった。<br>これらの結果は東京のワルファリン抵抗性個体群ではワルファリンの代謝・排泄能が亢進している事を示唆し、それによる迅速な解毒が抵抗性に寄与しているものと考えられる。
  • 石塚 真由美, 中山 翔太, 水川 葉月, 池中 良徳 日本毒性学会学術年会 43 (0) S16 -4 2016年 
    外来性化学物質代謝の第II相反応では、第I相反応の代謝物の極性をさらに増加させるグルクロン酸抱合(UGT)や硫酸抱合(SULT)、グルタチオン抱合酵素などの抱合反応が主要な代謝となっている。化学物質の代謝能力は、生物の化学物質に対する感受性を決定する重要なファクターである。我々はこれまでの研究から、魚類から哺乳類まで、P450および第II相反応の抱合に大きな動物種差があることを報告してきた。<br>哺乳類ではUGT1ファミリーは、主に内因性物質を代謝するビリルビングループ(1A1-1A5)と外因性物質を代謝するフェノールグループ(UGT1A6-1A10)に大別される。我々は、食肉目を中心とした哺乳類におけるUGT1ファミリーの遺伝子解析を行ったところ、鰭脚類は外因性物質であるアセトアミノフェン及び1-ヒドロキシピレンに対するUGT活性がネコと同程度に低く、中でもトドおよびキタオットセイではUGT1A6の偽遺伝子化が明らかになった。またUGT1ファミリーの系統解析及びシンテニー解析の結果、哺乳類のUGT1ファミリーは内因性物質代謝を担うUGT1A1相同遺伝子及び外因性物質代謝を担うUGT1A6相同遺伝子がそれぞれ遺伝子重複した後、動物種ごとに独自に遺伝子重複/欠損が起き、UGT1A2-1A5及びUGT1A7-1A10遺伝子が形成されたことが示唆された。一方、UGTと基質特異性が一部重複するSULTに関しては、これまで教科書的にブタで活性が欠損しているとされてきたが、ブタの肝臓ミクロソームのKm/Vmaxによる酵素効率の比較では他の動物種と大きな差はないことや、新たに硫酸抱合酵素が低活性である哺乳類を同定した<br>そこで、今回、動物が有する化学物質代謝の種差と毒性発現の違いを、UGTを中心に紹介したい。
  • 本平 航大, 池中 良徳, ワギ ダルウィシュ, 中山 翔太, 水川 葉月, 石塚 真由美 日本毒性学会学術年会 43 (0) P -84 2016年 
    【はじめに】AhR(芳香族炭化水素受容体)はシトクロムP450をはじめとした種々の薬物代謝酵素を誘導することで異物の代謝に関与する受容体として研究が進められてきた。近年、従来主要な役割であると考えられてきた異物の代謝に加え、成長因子、抗酸化因子、炎症物質などを誘導するといった新たな生体内での役割が注目を集めている。AhRは肝臓や肺といった異物代謝活性が高い組織で活性が高く、上記に挙げた新たなAhRの働きの研究がなされているのに対し、薬物の「蓄積」という特徴的な応答を示す脂肪組織における反応は未知の部分が多い。そこで本研究では脂肪組織におけるAhRの働きを探求するとともに他組織との比較を試みた。<br>【方法】脂肪組織としてヒト脂肪由来幹細胞、肝臓組織としてヒト肝癌由来細胞を用い、AhRのリガンドと考えられているβ-naphthoflavoneを含む複数の化学物質を暴露した。これらの暴露細胞から抽出、精製したcDNAを用いて比較リアルタイムPCR法により各組織における異物代謝酵素と成長因子をはじめとした異物代謝とは直積関与するわけではない遺伝子の発現量を暴露-被暴露間、肝-脂肪細胞間で比較した。<br>【結果と考察】AhRリガンド暴露の結果、ヒト脂肪由来幹細胞、ヒト肝癌細胞の何れの細胞においてもCYP1A1や成長因子である繊維芽細胞増殖因子(fgf2など)の発現量の増加が確認された。種々の遺伝子で脂肪由来幹細胞と肝癌細胞の間で反応性や遺伝子発現量に違いが確認された。この結果から一般的に肝臓で誘導が認められている遺伝子に関して、脂肪組織でも誘導されていることが明らかになった。また今回観察を行った繊維芽細胞増殖因子では複数のサブファミリーがAhRリガンドにより誘導されており、繊維芽細胞増殖因子の全てのサブファミリーがAhRの支配を受けていることが示唆された。本研究の結果、種々の遺伝子で脂肪由来幹細胞-肝癌細胞間に反応性や遺伝子発現量に差が見られ、これが脂肪組織におけるAhRの他組織における働きとの違いと推測出来た。
  • アクソルン サエンティエンチャイ, 池中 良徳, ネスタ サム, ウスマ ジャムナーク, 水川 葉月, 中山 翔太, 石塚 真由美 日本毒性学会学術年会 43 (0) P -237 2016年 
    African hedgehog, <i>Atalerix albiventris</i>, is a spiny mammal which becomes famous exotic pet in many counties. In order to elucidate xenobiotic metabolisms ability of hedgehogs, pyrene, one of the typical polycyclic aromatic hydrocarbon were exposed. From in vivo exposure study, pyrene was biotransformed to glucuronide and sulfate conjugates such as pyrene-1-glucuronide, pyrene-1-sulfate, and pyrenediol-sulfate and excreted to urine. Pyrene-1-glucuronide was main metabolite and limited excretion of sulfate conjugates were observed. Based on the results of in vivo exposure study, in vitro enzymatic kinetic experiments were performed by using various substrate and compare to rat and pig. Enzyme efficiency of cytochrome P450 (CYP) mediated Ethoxyresorufin <i>O</i>-deethylase activity, and warfarin 4'-, 6-, and 8-hydroxylation activity in hedgehogs were lower than that of rats. Furthermore, UDP-glucuronosyltransferase activities in hedgehogs were also lower <i>Km</i> than that of rats. Interestingly, the enzymatic efficiency of sulfation in hedgehogs was significantly lower than that of rats, whereas hedgehogs had similar sulfation ability as in pigs. Sulfation of hedgehogs may be limit activity in xenobiotic metabolism.
  • 水川 葉月, 高口 倖暉, 田辺 信介, 石塚 真由美, 前原 美咲, 横山 望, 市居 修, 滝口 満喜, 野見山 桂, 西川 博之, 池中 良徳, 中山 翔太 日本毒性学会学術年会 43 (0) P -6 2016年 
    ポリ塩化ビフェニル(PCBs)の水酸化代謝物であるOH-PCBsは、肝臓内で薬物代謝酵素より生成され、その後体外へ排泄される。しかしながら、一部の水酸化代謝物は甲状腺ホルモン(TH)と類似の構造をもつため、THの恒常性を撹乱することが危惧されている。これまでに、多様な陸棲哺乳類の血中OH-PCBsを分析したところ、種間でOH-PCBsの組成に差異が認められ、中でも、ネコのOH-PCBs残留パターンは他種と大きく異なることから、本種は特異な代謝機能を有することが示唆された。しかし、ネコの異物代謝能の研究は僅かであり、化学物質暴露による毒性影響も不明な点が多い。本研究では、ネコにおけるPCBs in vivo暴露試験を実施し、体内動態および代謝に関与する酵素活性や遺伝子を解析するとともに、化学物質の暴露評価に繋がる基盤的情報の収集を目的とした。
    コーン油に溶解した12異性体のPCBsを腹腔内投与し、経時採血した血清中PCBsおよびOH-PCBs濃度について同条件で実施したイヌのin vivo試験と比較した結果、異性体の残留パターンや体内動態にイヌとネコで種差が観察された。とくにネコでは低塩素化体の残留が顕著であった。また、代謝酵素活性および遺伝子解析の結果、PCBs暴露によりEROD、MROD、PROD活性は上昇するものの、第2相抱合酵素(UGTやSULT)活性は変化せず、PCBs暴露による抱合酵素活性への影響もみられなかった。また、CYP1A1およびCYP1A2遺伝子の発現量の上昇も認められた。
    本研究により、ネコのPCBs吸収・代謝・排泄能はイヌと異なることが示唆され、とくに低塩素化OH-PCBsの毒性リスクは高いことが予想された。低塩素化OH-PCBsは血中でTH輸送タンパクとの競合結合や、THの硫酸抱合排泄の阻害、TH起因性遺伝子の転写抑制などが報告されており、ネコの甲状腺機能障害が懸念される。
  • 石井千尋, 池中良徳, 市居修, 中山翔太, 西村紳一郎, 大橋哲, 田中誠一, 齊藤慶輔, 渡邊有希子, 水川葉月, 石塚真由美 日本野生動物医学会大会・講演要旨集 22nd 123 2016年
  • イヌ脳に及ぼすPCBsの影響 プロテオーム解析による評価
    高口 倖暉, 野見山 桂, Lauan Maria Claret, Yoo Jean, 水川 葉月, 岩田 久人, 国末 達也, 田辺 信介 環境ホルモン学会研究発表会要旨集 18回 106 -106 2015年12月
  • トランスクリプトーム解析によるPCBsを曝露したイヌへの影響評価
    田村 沙弥, 劉 珍, 水川 葉月, 阿草 哲郎, 平野 将司, 江口 哲史, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 環境ホルモン学会研究発表会要旨集 18回 116 -116 2015年12月
  • イヌ脳中PCBs、OH-PCBsの毒性影響評価 メタボロミクスアプローチ
    野見山 桂, 江口 哲史, 水川 葉月, Yoo Jean, 高口 倖暉, 山本 泰央, 国末 達也, 岩田 久人, 田辺 信介 環境ホルモン学会研究発表会要旨集 18回 117 -117 2015年12月
  • 山本恭央, 野見山桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺信介 日本内分泌かく乱化学物質学会研究発表会要旨集 18th 124 2015年12月
  • 森田鮎子, 池中良徳, 中山翔太, 石井千尋, 水川葉月, 渡邉研右, 齊藤慶輔, 渡邊有希子, 伊藤真輝, 高江洲昇, 石塚真由美 日本獣医学会学術集会講演要旨集 158th 433 2015年08月
  • 中山翔太, 水川葉月, 池中良徳, 石塚真由美 日本野生動物医学会大会・講演要旨集 21st 22 2015年07月
  • 山本恭央, 野見山桂, 水川葉月, 滝口満喜, 石塚真由美, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.1A‐20 2015年06月
  • 池中良徳, 筧麻友, 中山翔太, 川合佑典, 渡邊研右, 水川葉月, 野見山桂, 田辺信介, 石塚真由美 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.3A‐06 2015年06月
  • 武田一貴, 池中良徳, 田中和之, 中山翔太, 谷川力, 水川葉月, 石塚真由美 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.3A‐07 2015年06月
  • 水川葉月, 中山翔太, 池中良徳, 速水秋, DARWISH Wageh Sobhy, 渡邊研右, 川合佑典, 石塚真由美 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.P‐030 2015年06月
  • 石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 森田鮎子, 齊藤慶輔, 渡邊有希子, 神和夫, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.3D‐05 2015年06月
  • 野見山桂, 江口哲史, 水川葉月, YOO Jean, 高口倖暉, 山本泰央, 忍穂井智子, 岩田久人, 国末達也, 田辺信介 環境化学討論会要旨集(CD-ROM) 24th ROMBUNNO.1A‐06 2015年06月
  • 山田綾子, 堀本高矩, 石塚真由美, 池中良徳, 中山翔太, 水川葉月, 三谷曜子, 宮下和士 日本水産学会大会講演要旨集 2015 49 2015年03月
  • DARWISH Wageh Sobhy, IKENAKA Yoshinori, NAKAYAMA Shouta, MIZUKAWA Hazuki, ISHIZUKA Mayumi 日本毒性学会学術年会 42 (0) O -20 2015年 
    Benzo[a]pyeren (B[a]P) is one of the polycyclic aromatic hydrocarbons which is formed due to smoking of foods, incomplete combustion of woods, vehicle exhausts and cigarettes smokes. B[a]P gets entry into human and animal bodies mainly through their diets. B[a]P is confirmed to be pro-mutagenic and pro-carcinogenic to animals and humans in many studies. <br>Carotenoids such asβ-carotene and its metabolites like retinol and retinoic acids have a confirmed antioxidant activities through their radical scavenging roles. <br>The aim of this study, was firstly to estimate the B[a]P levels in fresh and heat treated meats. Additionally, B[a]P induced mutagenicity was studied using Salmonella mutagenicity assay. Moreover, B[a]P induced oxidative stress was examined using human hepatoma cell line (HepG2) cells after exposure to the relevant concentrations of B[a]P formed in heat-treated foods. In addition, the protective effects of carotenoids and retinoids against these harmful effects were also examined. The mechanisms behind these effects were also investigated.<br>The obtained results confirmed that carotenoids and retinoids have clear protective effects against B[a]P induced mutagenicity and oxidative stress, probably through their ability of induction of phase II and III enzymes and interference with the induction of phase I enzymes.
  • W. S. Darwish, W. S. Darwish, M. A. Hussein, K. I. El-Desoky, Y. Ikenaka, S. Nakayama, H. Mizukawa, M. Ishizuka International Food Research Journal 22 1719 -1726 2015年01月 
    Environmental pollution by heavy metals is a serious problem worldwide. This study aimed to investigate comparisons between concentrations of toxic metals (cadmium and lead) in the liver, kidneys, and muscle of Egyptian cattle and sheep. The effect of animal age on levels of toxic metal residues as well as results of a public health risk assessment is also reported. The results show that both cadmium and lead levels exceeded Food and Agriculture Organization and World Health Organization permissible limits in the liver and kidneys. These parts had higher concentrations of toxic metals compared with muscle in the two animal species examined. Age had a significant influence on toxic metal accumulation in both species. The hazard index indicates that consuming the livers and kidneys of these animals might pose a health risk.
  • 石塚真由美, 中山翔太, 水川葉月, 池中良徳 日本毒性病理学会講演要旨集 31st (JA)44,(EN)116 2015年
  • 池中 良徳, Aksorn SAENGTIENCHAI, 筧 麻友, Wageh Sobhy, DARWISH, 水川 葉月, 中山 翔太, 石塚 真由美 日本毒性学会学術年会 42 (0) S9 -3 2015年 
    化学物質に対する最も基本的な生体防御機構は異物代謝系である。一方、これら異物代謝系の動物種差が、各動物の化学物質に対する感受性に強く寄与することが知られている。各生物が持つ異物代謝系には大きな種差があることが報告されている。例えば、異物代謝・第II相抱合反応において、ネコではグルクロン酸抱合、イヌではアセチル抱合およびブタでは硫酸抱合遺伝子が欠損していることが獣医学領域の研究から明らかになっており、投薬時に注意を要する。しかし、これら異物代謝系の動物種差に関する知見は、一部の実験動物を除くとほとんどの生物種で十分に解明されていない。<br>我々の研究グループでは、野生動物を含む様々な動物の異物代謝・第II相抱合反応を解明するため、in vivo、in vitroレベルおよびゲノム情報を用いた評価法の構築を行った。その結果、グルクロン酸抱合活性が低いと言われているネコ科動物以外にも、フェレットやトド、北オットセイ等の野生動物でもその活性が低いことが明らかになってきた。一方、硫酸抱合活性が低いと言われてきたブタでは、その活性は代表的な実験動物であるラットと変化が無いことが明らかになった。
  • ポリ塩化ビフェニル(PCBs)曝露によるイヌ異物代謝酵素シトクロムP450への影響
    田村 沙弥, Yoo Jean, 水川 葉月, 阿草 哲郎, 平野 将司, 野見山 桂, 田辺 信介, 金 恩英, 岩田 久人 環境ホルモン学会研究発表会要旨集 17回 106 -106 2014年12月
  • 伊藤玄悟, 池内良徳, BEYENE Yared, 中山翔太, 水川葉月, VUREN Johannes, WEPENER Victor, SMIT Nico, WYNAND Vlok, 石塚真由美 日本獣医学会学術集会講演要旨集 157th 533 2014年08月
  • 中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, YARED YB, YABE John, 今内覚, 石塚真由美 日本獣医学会学術集会講演要旨集 157th 533 2014年08月
  • 石井千尋, 中山翔太, 池中良徳, 水川葉月, 中谷北斗, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 日本獣医学会学術集会講演要旨集 157th 533 2014年08月
  • 中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, YOHANNES Yared B, 今内覚, 石塚真由美 J Toxicol Sci 39 (Supplement) S336 -187 2014年07月 
    ケニア共和国の首都ナイロビ市内の大規模ゴミ集積場・ダンドラ地域は、世界第二位の巨大・高密度スラム街であり、地域内の子供の半数はWHO基準(100 μg/kg)以上の血中Pb濃度であることが報告されているが、家畜の汚染や生体への曝露源に関する報告はない。本研究では、ダンドラおよび対照区として同国・ナクル地域に生息するヤギ、ヒツジ、ブタおよびウシの血中金属類(10元素)濃度およびPb安定同位体比をICP-MSにより、PCBsおよび有機塩素系農薬(OCPs)濃度をGC-MSとGC-ECDにより測定した。<br> その結果、全てのサンプルでCr, Mn, Ni, Cu, Zn, As, Mo, Agの高濃度蓄積は認められず、PCBsおよびOCPsは検出限界以下であった。Pb濃度はナクルに比べてダンドラで高い蓄積傾向を示し、ヤギおよびヒツジでは有意差が認められた。ブタの平均Pb濃度はダンドラ(2600 μg/kg, dry wt:dw)がナクル(73 μg/kg, dw)の約35倍の高値を示した。ダンドラのウシからは、ヘム合成に関与するアミノレブリン酸脱水酵素活性が低下するとされる値(100μg/kg)と同程度のPb濃度(91 μg/kg以上)が検出され、血液毒性等の中毒症状の蔓延が示唆された。Cd濃度の地域差および種差は認められなかったが、総じて高値(570±460 ng/kg, dw)を示し、ウシの摂食によるヒトのCd曝露が示唆された。Pb安定同位体比は地域および動物種により異なる傾向を示し、地域内に複数の曝露源があること、動物種により主要な曝露源が異なることが示唆された。<br> 本研究より、両地域に生息する家畜には高濃度のPb, Cdが蓄積し、特にPb汚染はダンドラで深刻なレベルであり、その曝露源が複数存在することが示唆された。家畜と生活環境を共にするヒトへの同様の汚染も強く示唆された。
  • 石井千尋, 中山翔太, 池中良徳, 水川葉月, 中田北斗, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 J Toxicol Sci 39 (Supplement) S336 -188 2014年07月 
    【はじめに】北海道では、猛禽類において鉛弾摂取による鉛中毒が問題となっている。現在は鉛弾の使用規制が設けられているものの、未だに被害個体が発見されている。また、本州など他地域でも鉛中毒個体が発見されているが、北海道を除く地域における汚染実態調査は極僅かであり、鉛弾使用規制も不十分である。そこで、本研究では北海道、本州、四国において死亡した猛禽類の鉛濃度と鉛安定同位体比を測定し、鉛汚染状況と汚染源の解明を試みた。<br>【方法】試料は、1993年から2014年に北海道、本州、四国で死亡したイヌワシ、トビ、オオワシ、オオタカ、フクロウ、オジロワシ、オオハヤブサ、クマタカ、サシバ、ハイタカ、ツミ、チョウゲンボウの計49個体の肝臓を採取し、誘導結合プラズマ質量分析計で鉛濃度と鉛安定同位体比(<sup>207</sup>Pb/<sup>206</sup>Pb、<sup>208</sup>Pb/<sup>206</sup>Pb)を分析した。<br>【結果と考察】本研究の結果、1999‐2014年に北海道で死亡した4羽のオオワシにおいて、鉛中毒が疑われる値(11.0-29.5 mg/kg、湿重量)が検出された。北海道では2004年に大型獣の狩猟における鉛弾使用が禁止されているが、2013、2014年に採取されたオオワシにおいても高濃度の鉛蓄積が検出され、このうち1羽からはレントゲン撮影と剖検で胃内に鉛弾の破片が認められた。また岩手県で死亡したイヌワシからも高濃度の鉛蓄積(2.0 mg/kg)が認められた。さらに鉛安定同位体比を分析した結果、高濃度の鉛蓄積個体の鉛安定同位体比の値が同程度であった。このうち1羽から鉛弾が検出されたことを考慮すると、これらの高濃度蓄積個体の汚染源は鉛ライフル弾であると考えられた。<br> 上記の結果から、猛禽類の鉛弾摂取による中毒が未だに問題となっていることが明らかとなった。従って、北海道以外の地域における調査、また日本全国における鉛弾流通の完全禁止など規制強化の検討が必要であると考えられた。
  • 筧麻友, 中山翔太, 水川葉月, 池中良徳, 渡邊研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 J Toxicol Sci 39 (Supplement) S281 -78 2014年07月 
    【目的】グルクロン酸抱合酵素(UGT)は、生体外異物代謝の第Ⅱ相抱合反応を担い、各動物の化学物質感受性決定に関与することが報告されている。食肉目ネコ亜目(Feliformia)では環境化学物質や薬物等の代謝に関与するUGT1A6の偽遺伝子化が報告されており、この偽遺伝子化に伴いアセトアミノフェン等の薬物の毒性作用が強いことが知られている。一方、食肉目に属する鰭脚類(Pinnipedia)では、環境化学物質の高濃度蓄積が報告されているが、感受性に関与するUGTについての研究はほとんど行われていない。そこで、鰭脚類を中心とした食肉目において、肝臓でのUGT活性の測定と系統解析を行った。<br>【方法】食肉目に属するネコ(<i>Felis catus</i>)、イヌ(<i>Canis familiaris</i>)、鰭脚類であるトド(<i>Eumetopias jubatus</i>)、キタオットセイ(<i>Callorhinus ursinus</i>)、カスピカイアザラシ(<i>Phoca caspica</i>)及び対照としてラット(<i>Rattus norvegicus</i>)の肝臓ミクロソームを作成し、1-ヒドロキシピレン(UGT1A6、UGT1A7、UGT1A9)、アセトアミノフェン(UGT1A1、UGT1A6、UGT1A9)、セロトニン(UGT1A6)を基質としてUGT活性を測定した。また、NCBIのデータベースからUGT1A領域の系統解析およびシンテニー解析を行った。<br>【結果及び考察】1-ヒドロキシピレン、アセトアミノフェン、セロトニンに対するUGT抱合活性を測定した結果、ラットに比べ食肉目では極めて低い活性を示した。また、系統解析及びシンテニー解析より、解析した全ての食肉目において、UGT1A分子種は特徴的な2遺伝子であるUSP40 とMROH2の間に保存されていることが明らかになった。さらに、食肉目は齧歯目に比べUGT1A領域が短く、UGT1A分子種数が少ないことが確認された。以上の結果から、鰭脚類を含めた食肉目はUGTによる異物代謝能が低く、環境化学物質に対する感受性が高い可能性が考えられた。
  • 筧麻友, 池中良徳, 中山翔太, 水川葉月, 渡邊研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 J Toxicol Sci 39 (Supplement) S223 -9 2014年07月 
    【目的】グルクロン酸抱合酵素(UGT)は、異物代謝の第Ⅱ相抱合反応を担い、各動物の化学物質感受性決定に関与することが報告されている。食肉目ネコ亜目(<i>Feliformia</i>)では環境化学物質や薬物等の代謝に関与するUGT1A6の偽遺伝子化が報告されており、この偽遺伝子化によりアセトアミノフェン等の薬物の毒性作用が強く表れることが知られている。一方、食肉目に属する鰭脚類(<i>Pennipedia</i>)では、PCB、DDTなどの残留性有機汚染物質の高濃度蓄積が報告されているが、感受性に関与するUGTについての研究がほとんど行われていない。そこで、鰭脚類を中心とした食肉目において、UGTの機能解析及び系統解析を行い、種差を明らかにすることを目的とした。<br>【方法】食肉目に属するネコ(<i>Felis catus</i>)、イヌ(<i>Canis familiaris</i>)、鰭脚類であるトド(<i>Eumetopias jubatus</i>)、キタオットセイ(<i>Callorhinus ursinus</i>)、カスピカイアザラシ(<i>Phoca caspica</i>)及び対照としてラット(<i>Rattus norvegicus</i>)の肝臓ミクロソームを調整した。この肝臓ミクロソームを用いて、1-ヒドロキシピレンを基質としてUGT活性を測定した。さらに食肉目UGT1A遺伝子について、他の哺乳類と比較し、系統解析を行った。<br>【結果及び考察】1-ヒドロキシピレンを基質として用いたところ、イヌはラットと同程度のUGT活性を示したが、鰭脚類3種およびネコのUGT活性はラットの3分の1以下だった。また、UGT1A遺伝子は一般に2-10程度のホモログが近接して染色体上に存在しているが、系統解析及びシンテニー解析より、食肉目は齧歯目に比べUGT1A領域が短くUGT1A分子種数が少ないことが確認された。さらに、トドおよびキタオットセイでは、UGT1A6のエクソン1領域に2塩基挿入によるストップコドンが生じ、偽遺伝子化していることが明らかになった。以上の結果より、鰭脚類は、UGTによる異物代謝能が低く、環境化学物質に対する感受性が高い可能性が考えられた。
  • 水川葉月, 池中良徳, 野見山桂, 田辺信介, 宇根有美, 伊藤真輝, 高江洲昇, 長野靖子, 中山翔太, 石塚真由美 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3B-14 2014年05月
  • 石井千尋, 池中良徳, 中山翔太, 水川葉月, 齊藤慶輔, 渡邊有希子, 田辺信介, 野見山桂, 林光武, 増田泰, 坂本健太郎, 石塚真由美 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3D-03 2014年05月
  • 池中良徳, YOHANNES Yared Beyene, 中山翔太, 水川葉月, VAN VUREN Johannes J, WEPENER Victor, SMIT Nico J, WYNAND Vlok, 石塚真由美 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.2A-08 2014年05月
  • 中田北斗, 中山翔太, 水川葉月, 池中良徳, 石井千尋, YOHANNES Yared B, 今内覚, 石塚真由美 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3D-01 2014年05月
  • 江口哲史, 野見山桂, 落合真理, 水川葉月, 長野靖子, 神原千佳, 中川勝博, 田中幸樹, 宮川治彦, 田辺信介 環境化学討論会要旨集(CD-ROM) 23rd ROMBUNNO.3B-10 2014年05月
  • 筧麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 日本野生動物医学会大会・講演要旨集 20th 75 2014年
  • 筧麻友, 池中良徳, 中山翔太, 水川葉月, 渡邉研右, 坂本健太郎, 和田昭彦, 服部薫, 田辺信介, 野見山桂, 石塚真由美 日本分子生物学会年会プログラム・要旨集(Web) 37th 3P-0853 (WEB ONLY) 2014年
  • 水川葉月, 野見山桂, 中津賞, 久保田彰, 石塚真由美, 岩田久人, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-2D-7-3 2013年07月
  • 池中良徳, 田中和之, 中山翔太, 春成常仁, 谷川力, 水川葉月, 石塚真由美 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-2D-6-3 2013年07月
  • 長野靖子, 野見山桂, 水川葉月, 江口哲史, 中津賞, 田辺信介 環境化学討論会要旨集(CD-ROM) 22nd ROMBUNNO.3-2D-7-2 2013年07月
  • 野見山桂, 神原千佳, 水川葉月, 江口哲史, 落合真理, 磯部友彦, 山田格, 田辺信介 環境化学討論会要旨集(CD-ROM) 21st ROMBUNNO.1C-04 2012年07月
  • Accumulation Features of Organohalogen Metabolites in the Blood of Japanese Terrestrial Mammals.
    Mizukawa H, Nomiyama K, Nakatsu S, Yachimori S, Hayashi T, Tashiro Y, Yamamoto M, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 6 203 -210 2012年 [査読有り]
     
    Proceedings
  • Accumulation features of halogenated phenolic compounds in the blood of pinnipeds from Japanese coastal waters
    Nomiyama K, Kanbara C, Mizukawa H, Eguchi A, Ochiai M, Isobe T, Yamada T.K, Tanabe S Organohalogen Compounds 速報,短報,研究ノート等(学術雑誌) 74 931 -934 2012年 [査読有り]
     
    Proceedings
  • 野見山桂, 江口哲史, 村田仁子, 水川葉月, 染矢雅之, 磯部友彦, 山田格, 田辺信介 環境化学討論会要旨集(CD-ROM) 20th ROMBUNNO.2B-06 2011年07月
  • 神原千佳, 野見山桂, 水川葉月, 江口哲史, 磯部友彦, 山田格, 高橋真, 田辺信介 環境化学討論会要旨集(CD-ROM) 20th ROMBUNNO.P-053 2011年07月
  • 野見山桂, 江口哲史, 水川葉月, 磯部友彦, 山田格, 田辺信介 日本水環境学会シンポジウム講演集 13th 240 2010年09月
  • 野見山桂, 落合真理, 江口哲史, 水川葉月, 村田仁子, 山田格, 高橋真, 田辺信介 環境化学討論会講演要旨集 19th 248 -249 2010年06月
  • 落合真理, 野見山桂, 水川葉月, 江口哲史, 高橋真, 松石隆, 山田格, 田辺信介 環境化学討論会講演要旨集 19th 244 -245 2010年06月
  • Polychlorinated Biphenyls and Hydroxylated Polychlorinated Biphenyls in the Blood of Toothed and Baleen Whales Stranded along Japanese Coastal Waters.
    Nomiyama, K, Murata, S, Kunisue, T, Yamada, T.K, Mizukawa, H, Takahashi, S, Tanabe, S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 4 67 -74 2010年 [査読有り]
     
    Proceedings
  • Accumulation Features of Hydroxylated-PCBs (OH-PCBs) in the Blood of Pigs Collected from a Dumping Site for Municipal Wastes in India
    Mizukawa H, Nomiyama K, Kunisue T, Watanabe M.X, Subramanian A, Takahashi S, Tanabe S Interdisciplinary Studies on Environmental Chemistry 速報,短報,研究ノート等(学術雑誌) 4 175 -181 2010年 [査読有り]
     
    Proceedings

共同研究・競争的資金等の研究課題

  • ハウスダスト中化学物質プロファイル解明とペット動物を用いた健康リスク評価法の開発
    日本学術振興会:科学研究費助成事業 若手研究
    研究期間 : 2020年04月 -2022年03月 
    代表者 : 水川 葉月
  • mlAOPコンセプトによる化学物質感受性多様性評価と殺虫剤中毒解明に向けた応用
    日本学術振興会:科学研究費:基盤研究(A)
    研究期間 : 2018年04月 -2022年03月 
    代表者 : 池中 良徳
  • イオン性環境汚染物質がもたらす神経伝達物質への影響の理解と評価法の開発
    日本学術振興会:科学研究費助成事業 挑戦的研究(萌芽)
    研究期間 : 2019年06月 -2021年03月 
    代表者 : 野見山 桂, 水川 葉月, 池中 良徳
  • 高次の動物種における化学物質感受性の種差と適応進化の解明
    日本学術振興会:科学研究費:基盤研究(A)
    研究期間 : 2016年04月 -2020年03月 
    代表者 : 石塚 真由美
  • 野生動物iHep細胞の作成とトッププレデターのMetabolism評価法の開発
    日本学術振興会:科学研究費:挑戦的研究(萌芽)
    研究期間 : 2017年06月 -2019年03月 
    代表者 : 池中 良徳
  • オミックス解析によるイエネコの毒性発現機構の解明
    日本学術振興会:科学研究費:基盤研究(B)
    研究期間 : 2016年04月 -2019年03月 
    代表者 : 野見山 桂
  • 次世代型殺鼠剤開発を目的とした殺鼠剤抵抗性ラットの抵抗性獲得機序の解明
    日本学術振興会:科学研究費:挑戦的萌芽研究
    研究期間 : 2016年04月 -2018年03月 
    代表者 : 石塚 真由美
  • 農業用水を放射性物質高汚染地帯から流出する河川に依存する農地の汚染回避取水技術
    日本学術振興会:科学研究費:基盤研究(C)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 上野大介
  • ネコにおける有機ハロゲン化合物の代謝機構解明と甲状腺機能の影響評価
    日本学術振興会:科学研究費:若手研究(B)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 水川葉月
  • ベクターコントロールが及ぼす希少野生動物への影響評価と新規感受性評価法の開発
    日本学術振興会:科学研究費:基盤研究(B)
    研究期間 : 2015年04月 -2018年03月 
    代表者 : 池中良徳
  • 生体外異物代謝におけるグルクロン酸抱合の進化と細胞内デリバリーに対する機能解明
    日本学術振興会:科学研究費:挑戦的萌芽研究
    研究期間 : 2015年04月 -2017年03月 
    代表者 : 池中良徳
  • ベクターコントロールのための薬剤散布の生態系およびヒト健康に与える影響評価
    日本学術振興会:科学研究費:基盤研究(B)
    研究期間 : 2014年04月 -2017年03月 
    代表者 : 池中良徳
  • ペットのネコにおける有機ハロゲン代謝物の分析法開発と代謝機構解明およびリスク評価
    日本学術振興会:科学研究費:研究活動スタート支援
    研究期間 : 2013年08月 -2015年03月 
    代表者 : 水川葉月

愛媛大学教員活動実績

教育活動(B)

担当授業科目(B01)

  • 2019, 前期, 学部, 農学入門
  • 2019, 前期, 学部, 農学入門
  • 2019, 前期, 学部, 環境機器分析学


Copyright © MEDIA FUSION Co.,Ltd. All rights reserved.