大学院理工学研究科（工）
理工学専攻（電気電子工学）
English
更新日：2024/12/26
准教授
ｻｲﾄｳ　ﾀｶｼ
齋藤　卓

経歴

  1. 2010-2013大阪大学基礎工学研究科特任研究員
  2. 2013-2014愛媛大学医学系研究科研究員
  3. 2014/11-2019/10愛媛大学医学部附属病院助教
  4. 2019/11-2024/03愛媛大学大学院医学系研究科特任講師
  5. 2024/04-現在愛媛大学大学院理工学研究科准教授

学歴

  1. 筑波大学数理物質科学研究科博士前期課程修了2006
  2. 筑波大学数理物質科学研究科博士後期課程修了2009

学位

  1. 博士（理学）筑波大学2009/03
  2. 理学博士筑波大学

研究分野

  1. ライフサイエンス形態、構造顕微鏡技術・イメージング
  2. 情報通信生命、健康、医療情報学生体情報

研究キーワード

  1. メダカ
  2. シミュレーション
  3. 蛍光イメージング
  4. 画像解析
  5. バイオイメージング
  6. 数理生物学

共同研究・競争的資金等の研究課題

  1. 日本学術振興会科学研究費助成事業大規模高解像計測を実現する光シート顕微鏡による３次元デジタル病理解析技術の開発基盤研究(C)2024/04-2027/03競争的資金
  2. 【受託研究費】★創発的研究支援事業【齋藤卓】蛍光情報工学による高次元１分子計測テクノロジーの創出2024/04-2025/03競争的資金
  3. 公益財団法人 中谷医工計測振興財団中谷医工計測振興財団 令和４年度開発研究助成大規模高解像計測を実現する光シート顕微鏡を用いた浸潤性乳癌の３次元病理学の確立2023/04-2024/03競争的資金
  4. 【受託研究費】★創発的研究支援事業【齋藤卓】蛍光情報工学による高次元1分子計測テクノロジーの創出2023/04-2024/03競争的資金
  5. 公益財団法人 光科学技術研究振興財団光科学技術研究振興財団 令和４年度 研究助成多層化ライトシート顕微鏡の開発と生体内細胞移動過程の解明2023/01-2024/12競争的資金
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書籍等出版物

  1. ライトシート顕微鏡実践ガイド組織透明化＆ライブイメージングライブ長期観察のための2光子励起ライトシート顕微鏡羊土社2023/12/08978-4-7581-2268-9URL
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論文

  1. PKR associates with 4.1R to promote anchorage-independent growth of hepatocellular carcinoma and lead to poor prognosis.2024/11/13Yusuke Okujima Takao Watanabe Takeshi Ito Yasumichi Inoue Yutaka Kasai Yusuke Imai Yoshiko Nakamura Mitsuhito Koizumi Osamu Yoshida Yoshio Tokumoto Masashi Hirooka Masanori Abe Ryosuke Kawakami Takashi Saitou Takeshi Imamura Yoshinori Murakami Yoichi HiasaScientific reports14/ 1, 27768-27768研究論文（学術雑誌）10.1038/s41598-024-75142-5RNA-dependent protein kinase (PKR) may have a positive regulatory role in controlling tumor growth and progression in hepatocellular carcinoma (HCC). However, the downstream substrates and the molecular mechanism of PKR in the growth and progression of HCC have not been clarified. In this study, mass spectrometry analysis was performed with immunoprecipitated samples, and 4.1R was identified as a protein that binds to PKR. In transfected COS7 cells, an immunoprecipitation experiment showed that 4.1R binds to wild-type PKR, but not to a kinase-deficient mutant PKR, suggesting that PKR binds to 4.1R in a kinase activity-dependent manner. In HCC cell lines, HuH7 and HepG2, the expression level of 4.1R protein was shown to be regulated by protein expression and activation of PKR. Interestingly, high expression of 4.1R, as well as PKR, is associated with a worse prognosis in HCC. PKR increased HCC cell growth in both anchorage-dependent and anchorage-independent manners, whereas 4.1R was involved in HCC cell growth only in an anchorage-independent manner, not in an anchorage-dependent manner. The rescue experiment indicated that increased anchorage-independent growth of HCC cells by PKR might be caused by 4.1R. In conclusion, PKR associates with 4.1R and promotes anchorage-independent growth of HCC. The PKR-4.1R axis might be a new therapeutic target in HCC.
  2. Investigation of orientation behavior of nematic liquid crystals on UV-irradiated polyimide films2024/10/22Yuji Tsukamoto Hiroto Maeda Takashi Saitou Ryotaro Ozaki Masanori OZAKIJapanese Journal of Applied Physics研究論文（学術雑誌）10.35848/1347-4065/ad89c9URLURL_2IOP PublishingAbstract The orientation mechanism of liquid crystals (LCs) on surfaces remains unclear, despite several methods for controlling pretilt angles. This study investigates the relationship between the surface condition of polyimide films, whose pretilt angles can be controlled by UV dose, and LC orientation behavior. Absorbance at wavelength of 200 nm and 260 nm significantly decrease, while thickness reduces approximately 4 nm. A rubbing treatment further decreases the thickness by approximately 2 nm. Atomic force microscopy confirmed the change in molecular conformation by UV-irradiation and rubbing treatment. The dispersive and polar components of the surface free energy of UV-irradiated polyimide films are evaluated, it’s found that only the polar component changes with UV dose. Additionally, we confirm that the alignment of LCs transitions from homeotropic to planar with increased UV irradiation, demonstrating that pretilt angle distribution can be spatially controlled. These results contribute to establishing a photoalignment method for pretilt angle control.
  3. Enhanced light-sheet illumination by a multi-layered stair-step phase mask2024/10/01Takashi Saitou Takeshi ImamuraJapanese Journal of Applied Physics研究論文（学術雑誌）10.35848/1347-4065/ad83db<jats:title>Abstract</jats:title> <jats:p>Light-sheet microscopy is a valuable tool for live biological imaging, offering high speed, high spatial resolution, and low phototoxicity imaging. This study explores a multi-layered stair-step phase mask to further improve illumination of this microscopy. By inserting the mask into the illumination optics, we can modify the Gaussian beam to extend its length by approximately 1.6-fold, which increases the field of view in light-sheet microscopy while keeping resolution reduction small. We demonstrate this improvement through in vivo imaging of medaka fish embryos, highlighting the mask’s potential to enhance the visualization of multicellular organisms at the cellular level.</jats:p>
  4. Reinforced anti-myeloma therapy via dual-lymphoid activation mediated by a panel of antibodies armed with Bridging-BiTE2023/09/22Tatsuya Konishi Toshiki Ochi Masaki Maruta Kazushi Tanimoto Yukihiro Miyazaki Chika Iwamoto Takashi Saitou Takeshi Imamura Masaki Yasukawa Katsuto TakenakaBlood Journal研究論文（学術雑誌）10.1182/blood.2022019082URLAmerican Society of HematologyImmunotherapy using bispecific antibodies including bispecific T-cell engager (BiTE) has the potential to enhance the efficacy of treatment for relapsed/refractory multiple myeloma. However, myeloma may still recur after treatment due to downregulation of a target antigen and/or myeloma cell heterogeneity. To strengthen immunotherapy for myeloma while overcoming its characteristics, we have newly developed a BiTE-based modality, referred to as Bridging-BiTE (B-BiTE). B-BiTE was able to bind to both a human IgG-Fc domain and the CD3 molecule. Clinically available monoclonal antibodies (mAbs) were bound with B-BiTE prior to administration, and the mAb/B-BiTE complex induced antitumor T-cell responses successfully while preserving and supporting NK-cell reactivity, resulting in enhanced anti-myeloma effects via dual-lymphoid activation. In contrast, any unwanted off-target immune-cell reactivity mediated by mAb/B-BiTE complexes, or B-BiTE itself, appeared not to be observed in vitro and in vivo. Importantly, sequential immunotherapy using two different mAb/B-BiTE complexes appeared to circumvent myeloma cell antigen escape, and further augmented immune responses to myeloma relative to those induced by mAb/B-BiTE monotherapy or sequential therapy with two mAbs in the absence of B-BiTE. Therefore, this modality facilitates easy and prompt generation of a broad panel of bispecific antibodies that can induce deep and durable antitumor responses in the presence of clinically available mAbs, supporting further advancement of reinforced immunotherapy for multiple myeloma and other refractory hematological malignancies.
  5. Extended Depth of Focus Two-Photon Light-Sheet Microscopy for In Vivo Fluorescence Imaging of Large Multicellular Organisms at Cellular Resolution.2023/06/15Takashi Saitou Takeshi ImamuraInternational journal of molecular sciences24/ 12研究論文（学術雑誌）10.3390/ijms241210186Two-photon excitation in light-sheet microscopy advances applications to live imaging of multicellular organisms. In a previous study, we developed a two-photon Bessel beam light-sheet microscope with a nearly 1-mm field of view and less than 4-μm axial resolution, using a low magnification (10×), middle numerical aperture (NA 0.5) detection objective. In this study, we aimed to construct a light-sheet microscope with higher resolution imaging while maintaining the large field of view, using low magnification (16×) with a high NA 0.8 objective. To address potential illumination and detection mismatch, we investigated the use of a depth of focus (DOF) extension method. Specifically, we used a stair-step device composed of five-layer annular zones that extended DOF two-fold, enough to cover the light-sheet thickness. Resolution measurements using fluorescent beads showed that the reduction in resolutions was small. We then applied this system to in vivo imaging of medaka fish and found that image quality degradation at the distal site of the beam injection could be compensated. This demonstrates that the extended DOF system combined with wide-field two-photon light-sheet microscopy offers a simple and easy setup for live imaging application of large multicellular organism specimens with sub-cellular resolution.
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講演・口頭発表等

  1. 広視野２光子励起ライトシート顕微鏡の開発とライブイメージングへの応用第４６回日本分子生物学会年会2023/12/07口頭発表（招待・特別）
  2. 情報フォトニクスによる新しいバイオテクノロジー創出若手研究者リサーチユニット創生支援「情報フォトニクスによる次世代生命科学研究の創生」キックオフシンポジウム2023/03/20口頭発表（招待・特別）
  3. 先端イメージング技術を用いた医薬獣融合研究の探索医薬獣融合シンポジウム2023/02/21口頭発表（一般）
  4. 小型魚類をまるごと生きたまま観察できる２光子励起ライトシート顕微鏡光シートワークショップ2022/11/22
  5. Computational approach to development of non-linear optical microscopy and quantitative tumor imagingThe 81th Annual Meeting of the Japanese Cancer Association2022/09/30
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産業財産権

  1. 特許権焦点深度を伸長する顕微鏡用レンズの設計方法およびその製造方法特願2024-1542812024/09/06
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受賞

  1. 2022/10令和４年度愛媛大学学長賞
  2. 2022/10/04学長賞
  3. 2022/05令和３年度愛媛大学大学院医学系研究科長特別賞
  4. 2022/05/16愛媛大学大学院医学系研究科長特別賞Wide Field Light-Sheet Microscopy with Lens-axicon Controlled Two-photon Bessel Beam Illumination
  5. 2022/03第29回 源内奨励賞
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担当授業科目

  1. 2024電気電子工学実験Ⅱ
  2. 2024電気電子工学実験Ⅱ
  3. 2024バイオインフォマティクス特論
  4. 2024遺伝学
  5. 2024分子病態医学 講義・演習・実習
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メディア報道

  1. メダカ発生の様子を3日間まるごと撮影！生きている動物の中の細胞を見る技術～生体蛍光イメージング～インターネットメディアリケラボ2022/08/25
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担当経験のある科目

  1. 電気電子数学II愛媛大学工学部2024/04
  2. 生体医工学入門愛媛大学工学部2024/04
  3. 遺伝学愛媛大学医学部
  4. 基礎医学展望愛媛大学医学部
  5. 医科学研究愛媛大学医学部
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