地域協働推進機構
地域協働センター西条
English
更新日：2025/02/10
教授
ﾜﾀﾞ　ﾋﾛｼ
和田　博史

経歴

  1. 2004/11-2008/09カリフォルニア大学デイビス校ブドウ栽培醸造学部博士研究員
  2. 2008/10-2011/09農業・食品産業技術総合研究機構九州沖縄農業研究センター 水田作・園芸研究領域任期付研究員
  3. 2011/10-2013/09農業・食品産業技術総合研究機構九州沖縄農業研究センター 水田作・園芸研究領域主任研究員
  4. 2014/10-2018/09農業・食品産業技術総合研究機構九州沖縄農業研究センター 水田作研究領域主任研究員
  5. 2017/04-2018/02愛媛大学大学院農学研究科非常勤講師
  6. 2018/10-2020/03農業・食品産業技術総合研究機構九州沖縄農業研究センター 水田作研究領域上級研究員
  7. 2020/04-現在愛媛大学大学院農学研究科教授
  8. 2020/10/01愛媛大学植物工場研究センター教授（兼任）
  9. 2021/08-現在愛媛大学大学院農学研究科附属ハダカムギ開発研究センター教授（兼任）

学歴

  1. 愛媛大学1994/041998/03
  2. 愛媛大学大学院1998/042000/03
  3. 愛媛大学大学院連合農学研究科 博士課程2000/042004/09

学位

  1. 博士（農学）愛媛大学2004/09

教育・研究活動状況

植物生理学を専門にこれまでセルプレッシャープローブ・等圧式サイクロメーター等の植物水分生理計測器の改良と，１細胞代謝産物解析法（ピコリットル・プレッシャープローブ・エレクトロスプレーイオン化質量分析法，picoPPESI-MS）の開発を進めてきた．現在，picoPPESI-MS法の改良を進める一方，この細胞レベルのアプローチを水稲高温登熟障害（高温乾燥風に伴う乳白粒形成，高温に伴う背白粒発生・窒素追肥による白濁回避）を始めとする植物の環境ストレス応答の研究に応用することで，細胞生理学的な視点からメカニズムの解明を進めている．

研究分野

  1. 環境・農学作物生産科学
  2. 環境・農学園芸科学
  3. 環境・農学農業環境工学、農業情報工学

研究キーワード

  1. シングルセルオミクス
  2. セルプレッシャープローブ
  3. 質量分析
  4. 水分生理
  5. 植物生理
  6. 受粉
  7. 登熟
  8. 水稲
  9. ブドウ

共同研究・競争的資金等の研究課題

  1. 日本学術振興会科学研究費助成事業オンサイト１細胞分子計測による水稲高温不稔メカニズムの解明基盤研究(A)2022/04-2026/03競争的資金
  2. 日本学術振興会外国人研究者招へい事業オンサイト１細胞生体計測法による高等植物の受粉プロセスのメタボローム解析（日本学術振興会・外国人研究者招へい事業　外国人招へい研究者（短期），Prof. Rosa Erra-Balsells）外国人招へい研究者（短期），Prof. Rosa Erra-Balsells2025/04-2026/03競争的資金
  3. 科学技術振興機構　【受託研究費】遠隔での生育モニタリングによる密閉環境下の植物成長促進装置の検討A-STEPトライアウト2022/04-2023/03産学連携による資金
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書籍等出版物

  1. リンゴ「ふじ」のみつ入り果には揮発性成分の偏りと水ポテンシャル勾配が存在する田中福代，立木美保，和田博史農研機構 2021年研究成果情報2022/05URL
  2. みつ入りリンゴの不思議 ～果実内を流れる水の動き田中福代，和田博史果実日本，連載 果樹研究最前線 No. 300，日本園芸農業協同組合連合会，令和4年3月号2022/03/15
  3. 高温による背白粒発生と窒素追肥による抑制メカニズム和田博史最新農業技術，作物vol. 14，農文協2022/02/15
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論文

  1. Dynamics and stabilization mechanism of mitochondrial cristae morphofunction associated with turgor-driven cardiolipin biosynthesis under salt stress conditions.2022/07/01Nakata Keisuke,Hatakeyama Yuto,Erra-Balsells Rosa,Nonami Hiroshi,Wada HiroshiScientific reports12/ 1, 9727研究論文（学術雑誌）10.1038/s41598-022-14164-3Maintaining energy production efficiency is of vital importance to plants growing under changing environments. Cardiolipin localized in the inner mitochondrial membrane plays various important roles in mitochondrial function and its activity, although the regulation of mitochondrial morphology to various stress conditions remains obscure, particularly in the context of changes in cellular water relations and metabolisms. By combining single-cell metabolomics with transmission electron microscopy, we have investigated the adaptation mechanism in tomato trichome stalk cells at moderate salt stress to determine the kinetics of cellular parameters and metabolisms. We have found that turgor loss occurred just after the stress conditions, followed by the contrasting volumetric changes in mitochondria and cells, the accumulation of TCA cycle-related metabolites at osmotic adjustment, and a temporal increase in cardiolipin concentration, resulting in a reversible topological modification in the tubulo-vesicular cristae. Because all of these cellular events were dynamically observed in the same single-cells without causing any disturbance for redox states and cytoplasmic streaming, we conclude that turgor pressure might play a regulatory role in the mitochondrial morphological switch throughout the temporal activation of cardiolipin biosynthesis, which sustains mitochondrial respiration and energy conversion even under the salt stress conditions.
  2. Identification of growth‐related indicators affecting the appearance and protein content of rice grains2021/11/22Hiroshi Nakano Ryo Tanaka Senlin Guan Midori Okami Hiroshi Wada Makoto Hakata Hideki OhdanAgronomy Journal研究論文（学術雑誌）10.1002/agj2.20958URLWiley
  3. Direct evidence for dynamics of cell heterogeneity in watercored apples: turgor-associated metabolic modifications and within-fruit water potential gradient unveiled by single-cell analyses.2021/08/03Wada Hiroshi,Nakata Keisuke,Nonami Hiroshi,Erra-Balsells Rosa,Tatsuki Miho,Hatakeyama Yuto,Tanaka FukuyoHorticulture research8/ 1, 18710.1038/s41438-021-00603-1Watercore is a physiological disorder in apple (Malus × domestica Borkh.) fruits that appears as water-soaked tissues adjacent to the vascular core, although there is little information on what exactly occurs at cell level in the watercored apples, particularly from the viewpoint of cell water relations. By combining picolitre pressure-probe electrospray-ionization mass spectrometry (picoPPESI-MS) with freezing point osmometry and vapor pressure osmometry, changes in cell water status and metabolisms were spatially assayed in the same fruit. In the watercored fruit, total soluble solid was lower in the watercore region than the normal outer parenchyma region, but there was no spatial difference in the osmotic potentials determined with freezing point osmometry. Importantly, a disagreement between the osmotic potentials determined with two methods has been observed in the watercore region, indicating the presence of significant volatile compounds in the cellular fluids collected. In the watercored fruit, cell turgor varied across flesh, and a steeper water potential gradient has been established from the normal outer parenchyma region to the watercore region, retaining the potential to transport water to the watercore region. Site-specific analysis using picoPPESI-MS revealed that together with a reduction in turgor, remarkable metabolic modifications through fermentation have occurred at the border, inducing greater production of watercore-related volatile compounds, such as alcohols and esters, compared with other regions. Because alcohols including ethanol have low reflection coefficients, it is very likely that these molecules would have rapidly penetrated membranes to accumulate in apoplast to fill. In addition to the water potential gradient detected here, this would physically contribute to the appearance with high tissue transparency and changes in colour differences. Therefore, it is concluded that these spatial changes in cell water relations are closely associated with watercore symptoms as well as with metabolic alterations.
  4. Direct evidence for dynamics of cell heterogeneity in watercored apples: turgor-associated metabolic modifications and within-fruit water potential gradient unveiled by single-cell analyses2021/08/03Hiroshi Wada Keisuke Nakata Hiroshi Nonami Rosa Erra-Balsells Miho Tatsuki Yuto Hatakeyama Fukuyo TanakaHorticulture Research8/ 1研究論文（学術雑誌）10.1038/s41438-021-00603-1URLSpringer Science and Business Media LLC<title>Abstract</title>Watercore is a physiological disorder in apple (<italic>Malus</italic> <italic>×</italic> <italic>domestica</italic> Borkh.) fruits that appears as water-soaked tissues adjacent to the vascular core, although there is little information on what exactly occurs at cell level in the watercored apples, particularly from the viewpoint of cell water relations. By combining picolitre pressure-probe electrospray-ionization mass spectrometry (picoPPESI-MS) with freezing point osmometry and vapor pressure osmometry, changes in cell water status and metabolisms were spatially assayed in the same fruit. In the watercored fruit, total soluble solid was lower in the watercore region than the normal outer parenchyma region, but there was no spatial difference in the osmotic potentials determined with freezing point osmometry. Importantly, a disagreement between the osmotic potentials determined with two methods has been observed in the watercore region, indicating the presence of significant volatile compounds in the cellular fluids collected. In the watercored fruit, cell turgor varied across flesh, and a steeper water potential gradient has been established from the normal outer parenchyma region to the watercore region, retaining the potential to transport water to the watercore region. Site-specific analysis using picoPPESI-MS revealed that together with a reduction in turgor, remarkable metabolic modifications through fermentation have occurred at the border, inducing greater production of watercore-related volatile compounds, such as alcohols and esters, compared with other regions. Because alcohols including ethanol have low reflection coefficients, it is very likely that these molecules would have rapidly penetrated membranes to accumulate in apoplast to fill. In addition to the water potential gradient detected here, this would physically contribute to the appearance with high tissue transparency and changes in colour differences. Therefore, it is concluded that these spatial changes in cell water relations are closely associated with watercore symptoms as well as with metabolic alterations.
  5. Metabolic coordination of rice seed development to nighttime warming: in-situ determination of cellular redox states using picolitre pressure-probe electrospray-ionization mass spectrometry2021/05Fang-Yu Chang Yuto Hatakeyama Hiroshi Nonami Rosa Erra-Balsells Takuya Araki Hiroshi Nakano Hiroshi WadaEnvironmental and Experimental Botany188, 104515研究論文（学術雑誌）
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講演・口頭発表等

  1. 気候変動下の食料生産科学：細胞レベルの最先端アプローチ第4回愛媛の食農の未来とイノベーション シンポジウム20242024/12/14シンポジウム・ワークショップ パネル（指名）
  2. Metabolomics of lignin biosynthesis in developing xylem in Norway spruce: A trial for apoplastic solute analysis using centrifugation technique令和6年度愛媛大学農学研究科国際ワークショップ2024/12/11ポスター発表
  3. 水稲高温障害の発生要因と対策技術愛媛県補助事業水田農業競争力強化支援事業2024/12/05公開講演，セミナー，チュートリアル，講習，講義等
  4. 携帯型プレッシャーチャンバーを用いた圃場条件下におけるウンシュウミカンの樹体水分状態計測日本生物環境工学会2024年大阪大会2024/09/18口頭発表（一般）
  5. Identification of flavonoid compounds in rice stigma papillae using single-cell metabolomics日本生物環境工学会2024年大阪大会2024/09/18ポスター発表
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受賞

  1. 2021/05/20日本質量分析学会会誌賞Probe Electrospray Ionization (PESI) and Its Modified Versions: Dipping PESI (dPESI), Sheath-Flow PESI (sfPESI) and Adjustable sfPESI (ad-sfPESI)https://www.jstage.jst.go.jp/article/massspectrometry/9/1/9_A0092/_article
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担当授業科目

  1. 2024生物学入門
  2. 2024植物工場特論
  3. 2024植物工場特論
  4. 2024生物学入門
  5. 2024作物水分生理学
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メディア報道

  1. 学生も参加！次世代に繋げる！名人の栽培ノウハウをデータ化【株式会社アクト・ノード｜事業紹介インターネットメディアTRY ANGLE EHIME (トライアングル エヒメ）事務局TRY ANGLE EHIME（トライアングル エヒメ）公式HP2023/10/30
  2. インタビュー『蜜入りリンゴはどうやってできる!? 新たな代謝メカニズムを明らかにした独自の計測手法』インターネットメディアリケラボ（理系の理想のはたらき方を考える研究所）研究・論文（生物）インタビュー記事2023/03/03
  3. 塩ストレスに対する植物のエネルギー代謝の順化機構:１細胞スケールで見えてきた膨圧刺激によるカルジオリピン合成に同期したクリステの形態変化インターネットメディア愛媛大学プレスリリース2022/07/04
  4. 植物の環境応答の仕組みを細胞・分子レベルで明らかにする：水の流れ・代謝変化にフォーカスした1細胞生体計測法の改良と応用その他愛媛大学最先端研究紹介 infinity2021/12/24
  5. 蜜入りリンゴ謎解明：世界初、細胞レベルで幅広い成分が香り、味わいに　新聞・雑誌毎日新聞2021/11/17
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所属学協会

  1. 園芸学会
  2. 日本生物環境工学会
  3. 日本作物学会
  4. American Society of Plant Biologists
  5. Society for Experimental Biology
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委員歴

  1. 2023/04/01-現在愛媛県総合園芸振興審議会委員
  2. 2023/04/01-現在愛媛県科学技術振興会議農林水産評価専門部会委員
  3. 2022/04/01-現在日本作物学会英文誌 Plant Production Science 編集委員
  4. 2009/04/01-現在日本生物環境工学会英文誌 Environmental Control in Biology 編集委員
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